BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV40099 (840 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g05010.1 68418.m00532 clathrin adaptor complexes medium subun... 108 4e-24 At5g13760.1 68418.m01604 expressed protein similar to unknown pr... 30 2.2 At5g24530.1 68418.m02897 oxidoreductase, 2OG-Fe(II) oxygenase fa... 29 5.1 At4g24550.2 68417.m03519 clathrin adaptor complexes medium subun... 28 6.7 At4g24550.1 68417.m03518 clathrin adaptor complexes medium subun... 28 6.7 At1g66330.2 68414.m07533 senescence-associated family protein si... 28 6.7 At1g66330.1 68414.m07532 senescence-associated family protein si... 28 6.7 At1g58050.1 68414.m06579 helicase domain-containing protein cont... 28 6.7 >At5g05010.1 68418.m00532 clathrin adaptor complexes medium subunit-related contains pfam profile: PF00928 adaptor complexes medium subunit family Length = 527 Score = 108 bits (260), Expect = 4e-24 Identities = 52/105 (49%), Positives = 74/105 (70%) Frame = +3 Query: 204 YICRNRVRQICLPATRQLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQA 383 YI VR + P L++LL+TTK SNILEDL TL L S++VPEY + L E + + Sbjct: 48 YIETENVRYVYQPI-EALFLLLVTTKQSNILEDLATLTLLSKLVPEYSMSLDEEGISRAS 106 Query: 384 FNLLFAFDEIVALGYRESVNLAQVRSFVEMDSHEEKIYQAVRGPK 518 F L+FAFDE+++LG++ESV +AQV+ + EM+SHEEK+++ V K Sbjct: 107 FELIFAFDEVISLGHKESVTVAQVKQYCEMESHEEKLHKLVMQSK 151 Score = 91.9 bits (218), Expect = 5e-19 Identities = 39/62 (62%), Positives = 54/62 (87%) Frame = +1 Query: 67 MVLIAATVCTKSGKALVSRQFVEMTKARIEGLLAAFPKLMTGGRQHTFVETESVRYVYQP 246 MV++AA + KSGK +VSR +V+M++ RIEGLLAAFPKL+ G+QHT++ETE+VRYVYQP Sbjct: 1 MVVLAAAIVVKSGKVIVSRHYVDMSRIRIEGLLAAFPKLVGMGKQHTYIETENVRYVYQP 60 Query: 247 LD 252 ++ Sbjct: 61 IE 62 >At5g13760.1 68418.m01604 expressed protein similar to unknown protein (gb AAF63775.1) Length = 569 Score = 29.9 bits (64), Expect = 2.2 Identities = 13/41 (31%), Positives = 23/41 (56%) Frame = +3 Query: 306 ETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAFDEIVALGY 428 +T +F+ + L T+ N+ F LLF F ++VA+G+ Sbjct: 118 QTRSIFTPTPQQTLASLNSTKYTNKFFLLLFIFHKVVAIGF 158 >At5g24530.1 68418.m02897 oxidoreductase, 2OG-Fe(II) oxygenase family protein similar to flavanone 3-hydroxylase [Persea americana][GI:727410]; contains PF03171 2OG-Fe(II) oxygenase superfamily domain Length = 341 Score = 28.7 bits (61), Expect = 5.1 Identities = 9/26 (34%), Positives = 16/26 (61%) Frame = -2 Query: 374 QDFSFCELYTIFWYHSAEESQCLQIF 297 +DF++ E Y FW + ++ CL+ F Sbjct: 313 KDFTYAEYYKKFWSRNLDQEHCLENF 338 >At4g24550.2 68417.m03519 clathrin adaptor complexes medium subunit family protein contains Pfam profile: PF00928 adaptor complexes medium subunit family Length = 451 Score = 28.3 bits (60), Expect = 6.7 Identities = 15/56 (26%), Positives = 32/56 (57%), Gaps = 1/56 (1%) Frame = +3 Query: 303 LETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAF-DEIVALGYRESVNLAQVRSFV 467 LE L+ +RV+ +Y L E + + F L++ DE++ GY ++ + ++S++ Sbjct: 83 LELLQRIARVIKDYLGVLNE-DSFRKNFVLVYELLDEVIDFGYVQTTSTEVLKSYI 137 >At4g24550.1 68417.m03518 clathrin adaptor complexes medium subunit family protein contains Pfam profile: PF00928 adaptor complexes medium subunit family Length = 380 Score = 28.3 bits (60), Expect = 6.7 Identities = 15/56 (26%), Positives = 32/56 (57%), Gaps = 1/56 (1%) Frame = +3 Query: 303 LETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAF-DEIVALGYRESVNLAQVRSFV 467 LE L+ +RV+ +Y L E + + F L++ DE++ GY ++ + ++S++ Sbjct: 83 LELLQRIARVIKDYLGVLNE-DSFRKNFVLVYELLDEVIDFGYVQTTSTEVLKSYI 137 >At1g66330.2 68414.m07533 senescence-associated family protein similar to senescence-associated protein (GI:12836895) [Ipomoea batatas] Length = 417 Score = 28.3 bits (60), Expect = 6.7 Identities = 14/31 (45%), Positives = 22/31 (70%) Frame = -3 Query: 367 SVSVSCTQYSGTTLLKSLNVSRSSRMLLAFV 275 +++VS + L+KS+N SRS R+LLA+V Sbjct: 2 ALNVSKVVPNSPILVKSVNASRSRRVLLAYV 32 >At1g66330.1 68414.m07532 senescence-associated family protein similar to senescence-associated protein (GI:12836895) [Ipomoea batatas] Length = 417 Score = 28.3 bits (60), Expect = 6.7 Identities = 14/31 (45%), Positives = 22/31 (70%) Frame = -3 Query: 367 SVSVSCTQYSGTTLLKSLNVSRSSRMLLAFV 275 +++VS + L+KS+N SRS R+LLA+V Sbjct: 2 ALNVSKVVPNSPILVKSVNASRSRRVLLAYV 32 >At1g58050.1 68414.m06579 helicase domain-containing protein contains similarity to SP|P24785 Dosage compensation regulator (Male-less protein) (No action potential protein) {Drosophila melanogaster}; contains Pfam profiles PF04408: Helicase associated domain (HA2), PF00271: Helicase conserved C-terminal domain Length = 1417 Score = 28.3 bits (60), Expect = 6.7 Identities = 8/23 (34%), Positives = 15/23 (65%) Frame = -1 Query: 663 WRRRDIVELPKPLPKXICERGGW 595 W++ + ++PK L +C+R GW Sbjct: 387 WKKGEAQKIPKALLHQLCQRSGW 409 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 18,388,928 Number of Sequences: 28952 Number of extensions: 383553 Number of successful extensions: 1074 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 1045 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1074 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1941125600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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