BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV40070 (759 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC576.08c |rps2||40S ribosomal protein S2|Schizosaccharomyces ... 147 2e-36 SPAC23G3.08c |ubp7||ubiquitin C-terminal hydrolase Ubp7|Schizosa... 26 6.7 SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe... 26 6.7 SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S... 26 6.7 SPAPYUG7.03c |mid2||anillin homologue Mid2|Schizosaccharomyces p... 25 8.9 SPAC140.01 |sdh2||succinate dehydrogenase |Schizosaccharomyces p... 25 8.9 SPAC22E12.18 |||conserved fungal protein|Schizosaccharomyces pom... 25 8.9 SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosacchar... 25 8.9 >SPCC576.08c |rps2||40S ribosomal protein S2|Schizosaccharomyces pombe|chr 3|||Manual Length = 253 Score = 147 bits (356), Expect = 2e-36 Identities = 63/85 (74%), Positives = 78/85 (91%) Frame = +2 Query: 254 LDFFLGPSLNDEVLKIMPVQKQTRAGQRTRFKAFVAIGDNNGHIGLGVKCSKEVATAIRG 433 +D+FL P LNDEV+K++PVQKQTRAGQRTRFKAFV IGD++GH+GLG+KC+KEVATAIRG Sbjct: 68 VDYFL-PRLNDEVMKVVPVQKQTRAGQRTRFKAFVVIGDSDGHVGLGIKCAKEVATAIRG 126 Query: 434 AIILAKLSVLPVRRGYWGNKIGKPH 508 AII+ KLS++P+RRGYWG +G PH Sbjct: 127 AIIMGKLSIMPIRRGYWGTALGDPH 151 Score = 66.9 bits (156), Expect = 3e-12 Identities = 27/41 (65%), Positives = 37/41 (90%) Frame = +3 Query: 135 KEDQKEWVPVTKLGRLVREGKIDKLESIYLFSLPIKEFEIL 257 ++++KEWVPVTKLGRLV+ GKI +E IYL+SLPIKE++I+ Sbjct: 28 RDEEKEWVPVTKLGRLVKAGKIKSIEEIYLYSLPIKEYQIV 68 Score = 62.1 bits (144), Expect = 8e-11 Identities = 31/70 (44%), Positives = 42/70 (60%) Frame = +1 Query: 511 VPCKVTGKCGSVTIRLIPAPRGTGIVSAPIPKKLSFRWLVYRDATPQLVVQLAPWENLLK 690 VP KV+GKCGSVT+RL+PAPRG G+V+AP+ K+ + D Q N +K Sbjct: 153 VPVKVSGKCGSVTVRLVPAPRGAGLVAAPVTKRF-LQLAGIEDCYTQSRGSTKTLGNFVK 211 Query: 691 PTYAAIAKTY 720 +AA + TY Sbjct: 212 AAFAAASLTY 221 >SPAC23G3.08c |ubp7||ubiquitin C-terminal hydrolase Ubp7|Schizosaccharomyces pombe|chr 1|||Manual Length = 875 Score = 25.8 bits (54), Expect = 6.7 Identities = 15/44 (34%), Positives = 20/44 (45%) Frame = -1 Query: 642 SIPVHQPSERKLLRNWRRHNSSTTRGRNQPNCYRTTLAGDLARD 511 SIP+H PS R + R R H + +R P + D A D Sbjct: 554 SIPIHYPSSR-VSRRHRFHRALRSRFSRSPKKSSVKIVVDNAND 596 >SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe|chr 1|||Manual Length = 716 Score = 25.8 bits (54), Expect = 6.7 Identities = 19/51 (37%), Positives = 30/51 (58%) Frame = -2 Query: 299 SLEPHHSGTGRGRNQDLEFFDW*RKQVNALEFVDFSFANKTAEFGDRNPLF 147 +L PHH TG + + +D+ V++LE V + A +T E+ DRNPL+ Sbjct: 369 NLLPHHR-TGT-KYRAYPTYDFACPIVDSLEGV--THALRTTEYRDRNPLY 415 >SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 971 Score = 25.8 bits (54), Expect = 6.7 Identities = 10/22 (45%), Positives = 16/22 (72%) Frame = +1 Query: 601 PKKLSFRWLVYRDATPQLVVQL 666 P++ F+WL+ ATP+L+V L Sbjct: 67 PERSVFQWLIALTATPRLLVLL 88 >SPAPYUG7.03c |mid2||anillin homologue Mid2|Schizosaccharomyces pombe|chr 1|||Manual Length = 704 Score = 25.4 bits (53), Expect = 8.9 Identities = 14/45 (31%), Positives = 21/45 (46%) Frame = -3 Query: 460 NRQLSKDNSASNGSGDFLAALHTQTNMTVVVANGNKCLETCALSG 326 +R LS + GSG L N+T+ +A+G T +SG Sbjct: 373 SRNLSSSLQQTGGSGRLFVRLMEIRNLTIPLASGMTTRFTYTISG 417 >SPAC140.01 |sdh2||succinate dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 252 Score = 25.4 bits (53), Expect = 8.9 Identities = 10/31 (32%), Positives = 16/31 (51%) Frame = +3 Query: 492 RSESHTRPLQGHRQVWFCNNSADSCPSWYWN 584 +S + L G + C + SCPS++WN Sbjct: 151 QSRADRAKLDGLYECILCACCSTSCPSYWWN 181 >SPAC22E12.18 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 336 Score = 25.4 bits (53), Expect = 8.9 Identities = 13/32 (40%), Positives = 21/32 (65%) Frame = -3 Query: 274 RAEEEIKISNSLIGKENK*MLSSLSIFPSRTR 179 R+EE+I+++NSL+ K N + LSI R + Sbjct: 225 RSEEQIQLANSLLQKLNACKILFLSIKKRRIK 256 >SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 441 Score = 25.4 bits (53), Expect = 8.9 Identities = 15/36 (41%), Positives = 19/36 (52%) Frame = +2 Query: 575 VLELCLRQFLRSFLSDGWCTGMLHLSSWFNWHLGKI 682 V+ + F+ S LS WC LSSWFN L K+ Sbjct: 44 VISYAVLYFVNSLLS--WCM----LSSWFNSKLSKL 73 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,169,466 Number of Sequences: 5004 Number of extensions: 68843 Number of successful extensions: 174 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 166 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 173 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 363302114 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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