BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV40069 (752 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizos... 83 5e-17 SPBC17G9.04c |nup85||nucleoporin Nup85|Schizosaccharomyces pombe... 33 0.033 SPAC3C7.08c |elf1||AAA family ATPase ELf1|Schizosaccharomyces po... 30 0.41 SPAC14C4.02c |smc5|spr18|Smc5-6 complex SMC subunit Smc5 |Schizo... 29 0.94 SPAC1687.03c |rfc4||DNA replication factor C complex subunit Rfc... 28 1.2 SPBC530.04 |mod5||Tea1 anchoring protein Mod5|Schizosaccharomyce... 28 1.2 SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos... 28 1.2 SPAC20G4.03c |hri1||eIF2 alpha kinase Hri1|Schizosaccharomyces p... 28 1.6 SPAC14C4.13 |rad17||RFC related checkpoint protein Rad17|Schizos... 28 1.6 SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 27 2.2 SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schi... 27 2.2 SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar... 27 2.9 SPBC1706.03 |fzo1|SPBC839.01|mitochondrial fusion GTPase protein... 27 2.9 SPAC19A8.11c |||recombination protein Irc6 |Schizosaccharomyces ... 27 3.8 SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transpo... 27 3.8 SPAC23C4.02 |crn1||actin binding protein, coronin Crn1|Schizosac... 26 5.0 SPAC20G4.01 ||SPAC22F8.13|CCR4-Not complex subunit Caf16|Schizos... 26 6.6 SPBC14F5.06 |||iron-sulfur protein|Schizosaccharomyces pombe|chr... 26 6.6 SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||... 26 6.6 SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomy... 25 8.8 SPBC428.03c |pho4||thiamine-repressible acid phosphatase Pho4|Sc... 25 8.8 SPCC830.03 |||AAA family ATPase Grc3 |Schizosaccharomyces pombe|... 25 8.8 SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces... 25 8.8 SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch... 25 8.8 SPCC1322.06 |kap113||karyopherin Kap113|Schizosaccharomyces pomb... 25 8.8 >SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizosaccharomyces pombe|chr 1|||Manual Length = 1290 Score = 82.6 bits (195), Expect = 5e-17 Identities = 38/72 (52%), Positives = 52/72 (72%) Frame = +3 Query: 36 MAGVKSLAIRGIRSFGPEDTDEQRILFDSPLTLILGQNGCGKTTIIECLRYAITGQMPPG 215 M+ + ++I GIRSF ++ + I F SPLTLI+GQNG GKTTIIECL+YA TG +PP Sbjct: 1 MSCIDRMSIMGIRSF--DNRSRESIQFFSPLTLIVGQNGSGKTTIIECLKYATTGILPPN 58 Query: 216 SRNECFVHDAKV 251 ++ F+HD K+ Sbjct: 59 TKGGAFIHDPKI 70 Score = 63.3 bits (147), Expect = 4e-11 Identities = 27/74 (36%), Positives = 46/74 (62%) Frame = +1 Query: 508 CHQEDSSWPLDEGKKVKERFDEIFDADKYSDCLDRLKKIRKDYAQNLKLLEQEVAHLTEK 687 CHQE+S WPL E +K+RFDEIF++ +Y+ LD++K +++D +K+ + + H Sbjct: 155 CHQEESFWPLSEPANLKKRFDEIFESLRYAKALDQIKGLKRDQETQVKVDQATLTHYRSD 214 Query: 688 KPDLDQEKLNVVNS 729 K ++ +L V S Sbjct: 215 KERAEKIELRVHES 228 Score = 55.2 bits (127), Expect = 9e-09 Identities = 35/83 (42%), Positives = 53/83 (63%), Gaps = 1/83 (1%) Frame = +2 Query: 263 EVLGQVKLKIVNAKDKQLEVTRSMKVTALAKKKTKFQ-TLDSFLSTVDESGKTKDVSSRC 439 EVL QVKL N ++ TRS++++ KK T+ Q TLD L + ++ +T +S+RC Sbjct: 75 EVLAQVKLAFRNTNQVKMICTRSLQLSV--KKTTRQQKTLDGQLLILKDNERTT-ISNRC 131 Query: 440 ADLDILMHEELGVSKAILNSVVF 508 A+LD + LGVSKA+L+ V+F Sbjct: 132 AELDSQVPLSLGVSKALLDYVIF 154 >SPBC17G9.04c |nup85||nucleoporin Nup85|Schizosaccharomyces pombe|chr 2|||Manual Length = 675 Score = 33.5 bits (73), Expect = 0.033 Identities = 21/60 (35%), Positives = 33/60 (55%), Gaps = 5/60 (8%) Frame = +1 Query: 553 VKERFDEIFDADKYSDCLDRLKKIR--KDYAQNLKLLEQEVA---HLTEKKPDLDQEKLN 717 +KE DE+ D +YS C + K+IR D+ + KL +A H+ +K D+D E L+ Sbjct: 245 LKETVDELIDILRYSPCTHQ-KRIRSVSDFERRWKLWRSRLANLRHVVKKHRDIDSEVLD 303 >SPAC3C7.08c |elf1||AAA family ATPase ELf1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1057 Score = 29.9 bits (64), Expect = 0.41 Identities = 12/35 (34%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +3 Query: 135 ILGQNGCGKTTIIECL-RYAITGQMPPGSRNECFV 236 ++G NGCGK+T++ + Y + P CFV Sbjct: 475 VVGHNGCGKSTLLRAIGDYKVENFPSPDEVKTCFV 509 >SPAC14C4.02c |smc5|spr18|Smc5-6 complex SMC subunit Smc5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1065 Score = 28.7 bits (61), Expect = 0.94 Identities = 13/25 (52%), Positives = 17/25 (68%), Gaps = 1/25 (4%) Frame = +3 Query: 111 LFDSP-LTLILGQNGCGKTTIIECL 182 LF P L LI+G NG GK+TI+ + Sbjct: 29 LFPGPYLNLIIGPNGTGKSTIVSAI 53 >SPAC1687.03c |rfc4||DNA replication factor C complex subunit Rfc4|Schizosaccharomyces pombe|chr 1|||Manual Length = 342 Score = 28.3 bits (60), Expect = 1.2 Identities = 13/26 (50%), Positives = 16/26 (61%) Frame = +3 Query: 123 PLTLILGQNGCGKTTIIECLRYAITG 200 P +I G G GKTT I CL +A+ G Sbjct: 55 PHLVISGMPGIGKTTSILCLAHALLG 80 >SPBC530.04 |mod5||Tea1 anchoring protein Mod5|Schizosaccharomyces pombe|chr 2|||Manual Length = 522 Score = 28.3 bits (60), Expect = 1.2 Identities = 20/72 (27%), Positives = 31/72 (43%), Gaps = 1/72 (1%) Frame = +1 Query: 517 EDSSWPLDEGKKVKERFDEIFDADKYSDCLD-RLKKIRKDYAQNLKLLEQEVAHLTEKKP 693 +DSS P + K + D L+ + ++ + AQ K + V+HL Sbjct: 177 QDSSRPGRYSYRTKSASPALIDTSTLDSRLNFTMGRLERSIAQLSKNTMRAVSHLENPPK 236 Query: 694 DLDQEKLNVVNS 729 D+ KLNV NS Sbjct: 237 DITLPKLNVKNS 248 >SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizosaccharomyces pombe|chr 1|||Manual Length = 647 Score = 28.3 bits (60), Expect = 1.2 Identities = 11/21 (52%), Positives = 14/21 (66%) Frame = +3 Query: 126 LTLILGQNGCGKTTIIECLRY 188 +T++LG GCGKTT I Y Sbjct: 43 ITIVLGHTGCGKTTQIPQFLY 63 >SPAC20G4.03c |hri1||eIF2 alpha kinase Hri1|Schizosaccharomyces pombe|chr 1|||Manual Length = 704 Score = 27.9 bits (59), Expect = 1.6 Identities = 20/69 (28%), Positives = 38/69 (55%), Gaps = 2/69 (2%) Frame = -3 Query: 414 LPLSSTVERKESRV*NFVFFLAKAVTFMDLVTSNCLSFALTIFNFT*PSTSVDHSLLHH- 238 LP+SST + K+SR + LAK +F+ +++ + + N PS +V + L+H Sbjct: 116 LPMSSTKDSKKSRYSSPDGVLAKTASFLSVLSDG--GYEDDVMNVK-PSVNVLSNRLNHL 172 Query: 237 -EQSIRSCY 214 +++ SC+ Sbjct: 173 PVEALESCF 181 >SPAC14C4.13 |rad17||RFC related checkpoint protein Rad17|Schizosaccharomyces pombe|chr 1|||Manual Length = 606 Score = 27.9 bits (59), Expect = 1.6 Identities = 11/22 (50%), Positives = 16/22 (72%) Frame = +3 Query: 117 DSPLTLILGQNGCGKTTIIECL 182 +S L LI G +GCGK+T ++ L Sbjct: 104 ESRLLLICGPSGCGKSTAVQVL 125 >SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual Length = 4717 Score = 27.5 bits (58), Expect = 2.2 Identities = 11/26 (42%), Positives = 15/26 (57%) Frame = +3 Query: 105 RILFDSPLTLILGQNGCGKTTIIECL 182 R L L++G GCGKTT+ + L Sbjct: 1181 RCLLAKEPVLLVGDTGCGKTTVCQIL 1206 >SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3071 Score = 27.5 bits (58), Expect = 2.2 Identities = 11/17 (64%), Positives = 14/17 (82%) Frame = -2 Query: 652 TLSSVHNPFLFFLNDLS 602 T+ S HNPF+FF+N LS Sbjct: 2691 TVVSSHNPFVFFVNVLS 2707 >SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosaccharomyces pombe|chr 1|||Manual Length = 186 Score = 27.1 bits (57), Expect = 2.9 Identities = 11/27 (40%), Positives = 17/27 (62%) Frame = +3 Query: 132 LILGQNGCGKTTIIECLRYAITGQMPP 212 L+LG + GKTTI++CL ++ P Sbjct: 20 LLLGLDNAGKTTILKCLLNEDVNEVSP 46 >SPBC1706.03 |fzo1|SPBC839.01|mitochondrial fusion GTPase protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 758 Score = 27.1 bits (57), Expect = 2.9 Identities = 17/53 (32%), Positives = 25/53 (47%), Gaps = 2/53 (3%) Frame = +3 Query: 105 RILFDSPLTLILGQNGCGKTTIIECLRYA--ITGQMPPGSRNECFVHDAKVND 257 R+ S LI G GK+T+ L + + P + C VHDA++ND Sbjct: 149 RVEDTSSKILITGDLNAGKSTLCNALVHKDILPEDQQPCTEVFCEVHDAELND 201 >SPAC19A8.11c |||recombination protein Irc6 |Schizosaccharomyces pombe|chr 1|||Manual Length = 246 Score = 26.6 bits (56), Expect = 3.8 Identities = 13/37 (35%), Positives = 22/37 (59%) Frame = +3 Query: 132 LILGQNGCGKTTIIECLRYAITGQMPPGSRNECFVHD 242 L++G++ GK I+ A+TG +P G +E F +D Sbjct: 5 LVIGRSNSGKLDFIK----ALTGSLPKGLDDEAFNND 37 >SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transporter Bfr1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1530 Score = 26.6 bits (56), Expect = 3.8 Identities = 10/27 (37%), Positives = 17/27 (62%) Frame = +3 Query: 102 QRILFDSPLTLILGQNGCGKTTIIECL 182 Q + LT ++G++G GKTT++ L Sbjct: 905 QGFVVPGKLTALMGESGAGKTTLLNVL 931 >SPAC23C4.02 |crn1||actin binding protein, coronin Crn1|Schizosaccharomyces pombe|chr 1|||Manual Length = 601 Score = 26.2 bits (55), Expect = 5.0 Identities = 20/81 (24%), Positives = 34/81 (41%), Gaps = 7/81 (8%) Frame = +1 Query: 484 SHTKFCRICHQEDSSWPLDEGKKVKERFDEIFDADK-------YSDCLDRLKKIRKDYAQ 642 S K + + + PL E KKV+E+ DK +D R + K Y + Sbjct: 514 SEEKKLEVSDEAPKAAPLAESKKVEEKEPFYVSKDKKDISAVNLADLNKRFEGFEKRYEE 573 Query: 643 NLKLLEQEVAHLTEKKPDLDQ 705 L + + ++A L +K L + Sbjct: 574 ELAIRDWKIAQLEDKLAKLTE 594 >SPAC20G4.01 ||SPAC22F8.13|CCR4-Not complex subunit Caf16|Schizosaccharomyces pombe|chr 1|||Manual Length = 280 Score = 25.8 bits (54), Expect = 6.6 Identities = 9/18 (50%), Positives = 15/18 (83%) Frame = +3 Query: 129 TLILGQNGCGKTTIIECL 182 TL++G NG GK+T+++ L Sbjct: 33 TLLVGANGAGKSTLLKLL 50 >SPBC14F5.06 |||iron-sulfur protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 593 Score = 25.8 bits (54), Expect = 6.6 Identities = 9/17 (52%), Positives = 14/17 (82%) Frame = +3 Query: 117 DSPLTLILGQNGCGKTT 167 D+ + ++LG+NG GKTT Sbjct: 374 DAEIIVLLGENGTGKTT 390 >SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||Manual Length = 3971 Score = 25.8 bits (54), Expect = 6.6 Identities = 14/46 (30%), Positives = 27/46 (58%), Gaps = 1/46 (2%) Frame = -2 Query: 190 AYLKHSIIVVLPHPFCPNISVNGESNSMRCSSVSSGP-KLLIPLIA 56 +Y SII + P ++ VN + N++ +SVS+G +L +P ++ Sbjct: 52 SYSSTSIIHSVSSPVDCSLDVNNQINTINITSVSAGTNELFLPTLS 97 >SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomyces pombe|chr 1|||Manual Length = 754 Score = 25.4 bits (53), Expect = 8.8 Identities = 10/27 (37%), Positives = 19/27 (70%) Frame = +1 Query: 610 RLKKIRKDYAQNLKLLEQEVAHLTEKK 690 R KK++K+YAQ KL ++ ++++ K Sbjct: 727 RKKKLQKEYAQQEKLHKEYLSYIESNK 753 >SPBC428.03c |pho4||thiamine-repressible acid phosphatase Pho4|Schizosaccharomyces pombe|chr 2|||Manual Length = 463 Score = 25.4 bits (53), Expect = 8.8 Identities = 10/19 (52%), Positives = 14/19 (73%) Frame = +1 Query: 538 DEGKKVKERFDEIFDADKY 594 D G++V ER+ E+FD D Y Sbjct: 140 DLGRQVFERYYELFDTDVY 158 >SPCC830.03 |||AAA family ATPase Grc3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 736 Score = 25.4 bits (53), Expect = 8.8 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = +1 Query: 580 DADKYSDCLDRLKKIRKDYAQNLKLLEQEVAHL 678 D D Y C+ RL KDY N ++ ++E+ L Sbjct: 416 DPDHYIACVTRLFAEYKDYIFNQEISQKEIIPL 448 >SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1727 Score = 25.4 bits (53), Expect = 8.8 Identities = 18/72 (25%), Positives = 40/72 (55%), Gaps = 6/72 (8%) Frame = +1 Query: 547 KKVKERFDEIFDADKYSDCLDRLKKIRKDYAQNLKLLE---QEV---AHLTEKKPDLDQE 708 +K +E +++ + +++D L+K+R+DY + L+ +E +E+ A + E +P E Sbjct: 1056 RKTREDYEK--ELLRHADARSTLQKLREDYTKALEQVEDLNKEIALKAGINESQPFPISE 1113 Query: 709 KLNVVNSRVFFL 744 K + + V+ L Sbjct: 1114 KEDPLRQEVYVL 1125 >SPCC417.08 |tef3||translation elongation factor eEF3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1047 Score = 25.4 bits (53), Expect = 8.8 Identities = 13/36 (36%), Positives = 23/36 (63%) Frame = +3 Query: 135 ILGQNGCGKTTIIECLRYAITGQMPPGSRNECFVHD 242 ++G NG GK+T+I+ L TG++ P + E + H+ Sbjct: 705 VIGPNGAGKSTLIKVL----TGELLP-TVGEIYQHE 735 >SPCC1322.06 |kap113||karyopherin Kap113|Schizosaccharomyces pombe|chr 3|||Manual Length = 983 Score = 25.4 bits (53), Expect = 8.8 Identities = 18/61 (29%), Positives = 28/61 (45%), Gaps = 5/61 (8%) Frame = +1 Query: 577 FDADKYSDCLDRLKKIRKDYAQNLKLLEQEVAHLTEKKPDLDQ-----EKLNVVNSRVFF 741 FD YS CL + + + L Q +HL+ K ++ EKL +++ RVF Sbjct: 288 FDFSFYSPCLTKFPATIDYISLHYDFLGQISSHLSSYKEKFEESSKNFEKLVIMSLRVFI 347 Query: 742 L 744 L Sbjct: 348 L 348 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,225,536 Number of Sequences: 5004 Number of extensions: 69839 Number of successful extensions: 296 Number of sequences better than 10.0: 25 Number of HSP's better than 10.0 without gapping: 270 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 295 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 359287726 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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