Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV40047
(876 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 56 3e-08
At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 56 3e-08
At1g32190.1 68414.m03959 expressed protein 31 1.3
At5g15630.1 68418.m01829 phytochelatin synthetase family protein... 30 1.8
At1g23360.3 68414.m02922 UbiE/COQ5 methyltransferase family prot... 29 4.1
At1g23360.2 68414.m02921 UbiE/COQ5 methyltransferase family prot... 29 4.1
At1g23360.1 68414.m02923 UbiE/COQ5 methyltransferase family prot... 29 4.1
At5g56240.1 68418.m07018 expressed protein 29 5.4
At2g33010.1 68415.m04045 ubiquitin-associated (UBA)/TS-N domain-... 28 7.1
At2g02760.1 68415.m00219 ubiquitin-conjugating enzyme 2 (UBC2) E... 28 7.1
At1g14400.2 68414.m01708 ubiquitin-conjugating enzyme 1 (UBC1) E... 28 7.1
At1g14400.1 68414.m01707 ubiquitin-conjugating enzyme 1 (UBC1) E... 28 7.1
At4g14096.1 68417.m02176 F-box family protein contains F-box dom... 28 9.4
>At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative similar to elongation factor 1B
gamma GI:3868758 from [Oryza sativa]
Length = 413
Score = 56.0 bits (129), Expect = 3e-08
Identities = 24/61 (39%), Positives = 37/61 (60%), Gaps = 3/61 (4%)
Frame = +1
Query: 340 DSMPKGTFNMDDFKRVYSNEDE---AKSIPYFWEKFDPENYSIWYAEYKYPEELAKVFMT 510
D +P +DD+KR+YSN +I FW+ +DPE YS+W+ +YKY +E F+T
Sbjct: 258 DLLPPSPMVLDDWKRLYSNTKSNFREVAIKGFWDMYDPEGYSLWFCDYKYNDENMVSFVT 317
Query: 511 V 513
+
Sbjct: 318 L 318
Score = 38.7 bits (86), Expect = 0.005
Identities = 20/63 (31%), Positives = 31/63 (49%)
Frame = +2
Query: 2 TLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQVSAVVGSLTLCAAPPTYD 181
TLAD++ L F V+ S+ +V+R+F T+ +QP+ V+G A P
Sbjct: 157 TLADIVTICNLNLGFATVMTKKFTSAFPHVERYFWTMVNQPEFKKVLGDAKQTEAVPPVP 216
Query: 182 PKK 190
KK
Sbjct: 217 TKK 219
Score = 38.7 bits (86), Expect = 0.005
Identities = 20/73 (27%), Positives = 38/73 (52%)
Frame = +3
Query: 513 NLITGMFQRLDKMRKQAFASVCLFGEDNNSTISGVWVWRGKELVFPLSSDLAVDYEFLRL 692
N + G QR+D RK +F + + G + + G+W++RG E+ + ++ Y+
Sbjct: 319 NKVGGFLQRMDLARKYSFGKMLICGSEGPFKVKGLWLFRGPEIPKFIMDEV---YDMELY 375
Query: 693 EETWILSSEETKK 731
E T + S+E +K
Sbjct: 376 EWTKVDISDEAQK 388
>At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative Similar to elongation factor
1-gamma (gb|EF1G_XENLA). ESTs
gb|T20564,gb|T45940,gb|T04527 come from this gene
Length = 414
Score = 56.0 bits (129), Expect = 3e-08
Identities = 24/61 (39%), Positives = 37/61 (60%), Gaps = 3/61 (4%)
Frame = +1
Query: 340 DSMPKGTFNMDDFKRVYSNEDE---AKSIPYFWEKFDPENYSIWYAEYKYPEELAKVFMT 510
D +P +DD+KR+YSN +I FW+ +DPE YS+W+ +YKY +E F+T
Sbjct: 259 DLLPPSPMVLDDWKRLYSNTKSNFREVAIKGFWDMYDPEGYSLWFCDYKYNDENMVSFVT 318
Query: 511 V 513
+
Sbjct: 319 L 319
Score = 39.5 bits (88), Expect = 0.003
Identities = 22/63 (34%), Positives = 31/63 (49%)
Frame = +2
Query: 2 TLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQVSAVVGSLTLCAAPPTYD 181
TLAD+I L F V+ S +V+R+F TV +QP + V+G + A P
Sbjct: 157 TLADIITVCNLNLGFATVMTKKFTSEFPHVERYFWTVVNQPNFTKVLGDVKQTEAVPPIA 216
Query: 182 PKK 190
KK
Sbjct: 217 SKK 219
Score = 38.7 bits (86), Expect = 0.005
Identities = 20/73 (27%), Positives = 38/73 (52%)
Frame = +3
Query: 513 NLITGMFQRLDKMRKQAFASVCLFGEDNNSTISGVWVWRGKELVFPLSSDLAVDYEFLRL 692
N + G QR+D RK +F + + G + + G+W++RG E+ + ++ Y+
Sbjct: 320 NKVGGFLQRMDLARKYSFGKMLICGSEGPFKVKGLWLFRGPEIPKFIMDEV---YDMELY 376
Query: 693 EETWILSSEETKK 731
E T + S+E +K
Sbjct: 377 EWTKVDISDEAQK 389
>At1g32190.1 68414.m03959 expressed protein
Length = 422
Score = 30.7 bits (66), Expect = 1.3
Identities = 14/36 (38%), Positives = 16/36 (44%), Gaps = 1/36 (2%)
Frame = +1
Query: 550 CANRLSHPSACLVKTTIP-PSPECGCGAEKNSCSRC 654
C + L PS K P PS CGCG C +C
Sbjct: 296 CCSGLCRPSCSCPKPRCPKPSCSCGCGCGDCGCFKC 331
>At5g15630.1 68418.m01829 phytochelatin synthetase family protein /
COBRA cell expansion protein COBL4 similar to
phytochelatin synthetase [Hordeum vulgare subsp.
vulgare] GI:29570314; identified in Roudier, et al,
Plant Phys. (2002) 130:538-548 (PMID:12376623); contains
Pfam profile PF04833: Phytochelatin synthetase-like
conserved region
Length = 431
Score = 30.3 bits (65), Expect = 1.8
Identities = 15/32 (46%), Positives = 16/32 (50%), Gaps = 6/32 (18%)
Frame = +1
Query: 568 HPSACL-----VKTTIPPSPECGCGAE-KNSC 645
HPS C+ TI P P C CG E K SC
Sbjct: 214 HPSCCVSFSSFYNDTITPCPSCACGCENKKSC 245
>At1g23360.3 68414.m02922 UbiE/COQ5 methyltransferase family protein
similar to 2-hexaprenyl-1,4-naphthoquinone
methyltransferase GB:BAA25267 GI:2982680 from
[Micrococcus luteus]; contains Pfam profile PF01209:
methlytransferase, UbiE/COQ5 family
Length = 160
Score = 29.1 bits (62), Expect = 4.1
Identities = 16/57 (28%), Positives = 30/57 (52%), Gaps = 2/57 (3%)
Frame = +3
Query: 570 SVCLFGEDNNSTISGVWVWRGKELVFPLSS--DLAVDYEFLRLEETWILSSEETKKI 734
S+ F + N S + + W +V P+++ DLA +YE+L+ L+ EE + +
Sbjct: 78 SILDFNKSNQSVTTFMQGWMIDNVVVPVATVYDLAKEYEYLKYSINGYLTGEELETL 134
>At1g23360.2 68414.m02921 UbiE/COQ5 methyltransferase family protein
similar to 2-hexaprenyl-1,4-naphthoquinone
methyltransferase GB:BAA25267 GI:2982680 from
[Micrococcus luteus]; contains Pfam profile PF01209:
methlytransferase, UbiE/COQ5 family
Length = 160
Score = 29.1 bits (62), Expect = 4.1
Identities = 16/57 (28%), Positives = 30/57 (52%), Gaps = 2/57 (3%)
Frame = +3
Query: 570 SVCLFGEDNNSTISGVWVWRGKELVFPLSS--DLAVDYEFLRLEETWILSSEETKKI 734
S+ F + N S + + W +V P+++ DLA +YE+L+ L+ EE + +
Sbjct: 78 SILDFNKSNQSVTTFMQGWMIDNVVVPVATVYDLAKEYEYLKYSINGYLTGEELETL 134
>At1g23360.1 68414.m02923 UbiE/COQ5 methyltransferase family protein
similar to 2-hexaprenyl-1,4-naphthoquinone
methyltransferase GB:BAA25267 GI:2982680 from
[Micrococcus luteus]; contains Pfam profile PF01209:
methlytransferase, UbiE/COQ5 family
Length = 198
Score = 29.1 bits (62), Expect = 4.1
Identities = 16/57 (28%), Positives = 30/57 (52%), Gaps = 2/57 (3%)
Frame = +3
Query: 570 SVCLFGEDNNSTISGVWVWRGKELVFPLSS--DLAVDYEFLRLEETWILSSEETKKI 734
S+ F + N S + + W +V P+++ DLA +YE+L+ L+ EE + +
Sbjct: 116 SILDFNKSNQSVTTFMQGWMIDNVVVPVATVYDLAKEYEYLKYSINGYLTGEELETL 172
>At5g56240.1 68418.m07018 expressed protein
Length = 986
Score = 28.7 bits (61), Expect = 5.4
Identities = 21/82 (25%), Positives = 36/82 (43%)
Frame = +2
Query: 608 LRSVGVARKRTRVPAVV*FGSGLRVPTTGRNLDPFERGDQENFSKTTSSWERNRQKTGRK 787
L S RK+T + RVP + + + E + K+ +S++ ++ G+K
Sbjct: 457 LGSEVAGRKKTLFRSQSAQSRSFRVPQSEKEDESLEGVNINRLKKSVASFQEDKYPPGKK 516
Query: 788 FQPRARYFKWNSRQKFKPMFYP 853
F A + S +KF P F P
Sbjct: 517 FCSDASP-RGTSMRKFSPPFVP 537
>At2g33010.1 68415.m04045 ubiquitin-associated (UBA)/TS-N
domain-containing protein contains Pfam profile PF00627:
UBA/TS-N domain
Length = 649
Score = 28.3 bits (60), Expect = 7.1
Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = +2
Query: 386 STPTKTKLNLFLTSGRSLTPRTIPFGMPNT-NTLRNSL 496
+ PT L ++L G S+TP IPF T +RN++
Sbjct: 565 AAPTTITLTIYLDDGGSVTPVYIPFRSDQTVRDIRNAI 602
>At2g02760.1 68415.m00219 ubiquitin-conjugating enzyme 2 (UBC2) E2;
identical to gi:2689242, SP:P42745
Length = 152
Score = 28.3 bits (60), Expect = 7.1
Identities = 12/38 (31%), Positives = 16/38 (42%)
Frame = -3
Query: 643 TSSFPRHTHTPEMVELLSSPNRQTDAKACLRILSNLWN 530
+ +P T V + PN D CL IL N W+
Sbjct: 60 SEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWS 97
>At1g14400.2 68414.m01708 ubiquitin-conjugating enzyme 1 (UBC1) E2;
identical to gi:431259, SP:P25865
Length = 152
Score = 28.3 bits (60), Expect = 7.1
Identities = 12/38 (31%), Positives = 16/38 (42%)
Frame = -3
Query: 643 TSSFPRHTHTPEMVELLSSPNRQTDAKACLRILSNLWN 530
+ +P T V + PN D CL IL N W+
Sbjct: 60 SEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWS 97
>At1g14400.1 68414.m01707 ubiquitin-conjugating enzyme 1 (UBC1) E2;
identical to gi:431259, SP:P25865
Length = 152
Score = 28.3 bits (60), Expect = 7.1
Identities = 12/38 (31%), Positives = 16/38 (42%)
Frame = -3
Query: 643 TSSFPRHTHTPEMVELLSSPNRQTDAKACLRILSNLWN 530
+ +P T V + PN D CL IL N W+
Sbjct: 60 SEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWS 97
>At4g14096.1 68417.m02176 F-box family protein contains F-box domain
Pfam:PF00646
Length = 468
Score = 27.9 bits (59), Expect = 9.4
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +2
Query: 365 TWMISSVSTPTKTKLNLFLTSGRSLTPRTIPFGMPN 472
TW +SVS PT +L F T R P+++ PN
Sbjct: 199 TWDFASVSVPTLKRLT-FSTQVRDEFPKSVSIDTPN 233
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,901,336
Number of Sequences: 28952
Number of extensions: 441879
Number of successful extensions: 1415
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 1357
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1412
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 2058178400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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