Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV31052
(682 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 144 5e-35
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 144 5e-35
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 144 5e-35
At4g10710.1 68417.m01751 transcriptional regulator-related simil... 31 0.71
At1g29760.1 68414.m03639 expressed protein 31 0.71
At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 29 2.8
At1g14210.1 68414.m01682 ribonuclease T2 family protein contains... 29 2.8
At4g38880.1 68417.m05506 amidophosphoribosyltransferase, putativ... 29 3.8
At3g56260.1 68416.m06252 expressed protein 28 5.0
At4g03090.1 68417.m00417 expressed protein 28 6.6
At5g63000.1 68418.m07904 expressed protein 27 8.7
At5g52440.1 68418.m06507 HCF106 protein identical to HCF106 [Ara... 27 8.7
At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 27 8.7
At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 27 8.7
At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 27 8.7
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 144 bits (349), Expect = 5e-35
Identities = 65/84 (77%), Positives = 73/84 (86%)
Frame = +3
Query: 3 GPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLK 182
G KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+
Sbjct: 4 GLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVIS 63
Query: 183 IVKQRLIKVDGKVRTDPTYPAGFM 254
I+ QR I+VDGKVRTD TYPAGFM
Sbjct: 64 ILMQRHIQVDGKVRTDKTYPAGFM 87
Score = 127 bits (307), Expect = 6e-30
Identities = 58/94 (61%), Positives = 70/94 (74%)
Frame = +2
Query: 254 DVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTI 433
DVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRTI
Sbjct: 88 DVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTI 147
Query: 434 RYPDPLIKVNDSIQLDIATTKIMDFSSLSPGTCV 535
RYPDPLIK ND+I+LD+ KI++F G V
Sbjct: 148 RYPDPLIKPNDTIKLDLEANKIVEFIKFDVGNVV 181
Score = 90.2 bits (214), Expect = 1e-18
Identities = 38/58 (65%), Positives = 48/58 (82%)
Frame = +1
Query: 508 LKFESGNLCMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGK 681
+KF+ GN+ M+TGGRN GRVG I +RE+H GSF+ +HI+DSTGH FATRL NV+ IGK
Sbjct: 173 IKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIGK 230
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 144 bits (349), Expect = 5e-35
Identities = 65/84 (77%), Positives = 73/84 (86%)
Frame = +3
Query: 3 GPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLK 182
G KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+
Sbjct: 4 GLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVIS 63
Query: 183 IVKQRLIKVDGKVRTDPTYPAGFM 254
I+ QR I+VDGKVRTD TYPAGFM
Sbjct: 64 ILMQRHIQVDGKVRTDKTYPAGFM 87
Score = 127 bits (306), Expect = 8e-30
Identities = 58/94 (61%), Positives = 70/94 (74%)
Frame = +2
Query: 254 DVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTI 433
DVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRTI
Sbjct: 88 DVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTI 147
Query: 434 RYPDPLIKVNDSIQLDIATTKIMDFSSLSPGTCV 535
RYPDPLIK ND+I+LD+ KI++F G V
Sbjct: 148 RYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVV 181
Score = 90.2 bits (214), Expect = 1e-18
Identities = 38/58 (65%), Positives = 48/58 (82%)
Frame = +1
Query: 508 LKFESGNLCMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGK 681
+KF+ GN+ M+TGGRN GRVG I +RE+H GSF+ +HI+DSTGH FATRL NV+ IGK
Sbjct: 173 IKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIGK 230
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 144 bits (349), Expect = 5e-35
Identities = 65/84 (77%), Positives = 73/84 (86%)
Frame = +3
Query: 3 GPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLK 182
G KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+
Sbjct: 4 GLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVIS 63
Query: 183 IVKQRLIKVDGKVRTDPTYPAGFM 254
I+ QR I+VDGKVRTD TYPAGFM
Sbjct: 64 ILMQRHIQVDGKVRTDKTYPAGFM 87
Score = 127 bits (306), Expect = 8e-30
Identities = 58/94 (61%), Positives = 70/94 (74%)
Frame = +2
Query: 254 DVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTI 433
DVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRTI
Sbjct: 88 DVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTI 147
Query: 434 RYPDPLIKVNDSIQLDIATTKIMDFSSLSPGTCV 535
RYPDPLIK ND+I+LD+ KI++F G V
Sbjct: 148 RYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVV 181
Score = 90.2 bits (214), Expect = 1e-18
Identities = 38/58 (65%), Positives = 48/58 (82%)
Frame = +1
Query: 508 LKFESGNLCMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGK 681
+KF+ GN+ M+TGGRN GRVG I +RE+H GSF+ +HI+DSTGH FATRL NV+ IGK
Sbjct: 173 IKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIGK 230
>At4g10710.1 68417.m01751 transcriptional regulator-related similar
to chromatin-specific transcription elongation factor
FACT 140 kDa subunit (GI:5499741) [Homo sapiens]
Length = 1074
Score = 31.1 bits (67), Expect = 0.71
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -1
Query: 118 KHSRNLWGPVDGLGAYTPPSLSNIHAL 38
KHS +LWG D L TPP+ ++ L
Sbjct: 44 KHSADLWGSADALAIATPPASDDLRYL 70
>At1g29760.1 68414.m03639 expressed protein
Length = 526
Score = 31.1 bits (67), Expect = 0.71
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -1
Query: 229 SVLTFPSTFMRRCFTIFRTSFPVKAYFRRFL 137
S+LTFP +R CF F F + RRFL
Sbjct: 195 SLLTFPPWLLRNCFLFFFDPFSTIRFGRRFL 225
>At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase,
putative / steroid 5-alpha-reductase, putative identical
to gi:1280611; contains a steroid 5-alpha reductase,
C-terminal domain
Length = 262
Score = 29.1 bits (62), Expect = 2.8
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -1
Query: 208 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 92
TF R C + P A +FL+ GKH+R WGP
Sbjct: 8 TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45
>At1g14210.1 68414.m01682 ribonuclease T2 family protein contains
similarity to RNase GI:7768564 from [Nicotiana tabacum];
contains Pfam profile PF00445: Ribonuclease T2 family
Length = 247
Score = 29.1 bits (62), Expect = 2.8
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +2
Query: 491 TKIMDFSSLSPGTCV*SREAVTWGVWAPSCPARD 592
T + D S +S C ++ WGVWA CP+ +
Sbjct: 79 TNLFDISKISDLVCQMEKKWTEWGVWA--CPSNE 110
>At4g38880.1 68417.m05506 amidophosphoribosyltransferase, putative /
glutamine phosphoribosylpyrophosphate amidotransferase,
putative / phosphoribosyldiphosphate 5-amidotransferase,
putative similar to amidophosphoribosyltransferase
[Arabidopsis thaliana] GI:469195; contains Pfam profiles
PF00156: Phosphoribosyl transferase domain, PF00310:
Glutamine amidotransferases class-II
Length = 532
Score = 28.7 bits (61), Expect = 3.8
Identities = 28/91 (30%), Positives = 40/91 (43%)
Frame = +1
Query: 394 ECSVLGNA*WPHHPLSRPTYQSQRFHPVRHCNYEDYGLLKFESGNLCMITGGRNLGRVGT 573
EC V+G P SR +Y + H ++H E G++ L ITG + V T
Sbjct: 76 ECGVVGIHGDPE--ASRLSYLA--LHALQHRGQEGAGIVAANQNGLESITGVGLVSDVFT 131
Query: 574 IVSRERHPGSFDIVHIKDSTGHTFATRLNNV 666
PG I H++ ST + A+ L NV
Sbjct: 132 ESKLNNLPGDIAIGHVRYST--SGASMLKNV 160
>At3g56260.1 68416.m06252 expressed protein
Length = 176
Score = 28.3 bits (60), Expect = 5.0
Identities = 21/73 (28%), Positives = 35/73 (47%), Gaps = 2/73 (2%)
Frame = +1
Query: 424 PHHPLSRPTYQSQRFHPVRHCNYEDYGLLKFESGNLCMITGGRNLGRVGTI--VSRERHP 597
P H L + ++ P+ + NYE+ L + LC GGR LG ++ V+R
Sbjct: 95 PPHSLKKRMSHDKK-SPLLYANYEEDELRSSPTSTLCCSNGGR-LGCSSSMGSVTRALFS 152
Query: 598 GSFDIVHIKDSTG 636
GS + + ++TG
Sbjct: 153 GSMEGMKRHETTG 165
>At4g03090.1 68417.m00417 expressed protein
Length = 877
Score = 27.9 bits (59), Expect = 6.6
Identities = 13/35 (37%), Positives = 21/35 (60%)
Frame = +3
Query: 168 NEVLKIVKQRLIKVDGKVRTDPTYPAGFMMLSQLR 272
+EVLK+++ L K P YP GF++L+ +R
Sbjct: 318 SEVLKLLRLGLSKAS-MATASPDYPMGFVLLNAMR 351
>At5g63000.1 68418.m07904 expressed protein
Length = 201
Score = 27.5 bits (58), Expect = 8.7
Identities = 18/48 (37%), Positives = 23/48 (47%)
Frame = -2
Query: 636 SRGVLDVHNVEGAGMSLAGHDGAHTPQVTASRDHTQVPGLKLEKSIIF 493
+RGV DV NV GAG + A G P A R + G L ++ F
Sbjct: 110 TRGVHDVFNVVGAGSATAAVFGLIMPGSLAWRARNVLLGSVLGATVCF 157
>At5g52440.1 68418.m06507 HCF106 protein identical to HCF106
[Arabidopsis thaliana] GI:4894914; contains Pfam profile
PF02416: mttA/Hcf106 family
Length = 260
Score = 27.5 bits (58), Expect = 8.7
Identities = 22/94 (23%), Positives = 38/94 (40%), Gaps = 1/94 (1%)
Frame = +2
Query: 386 GPKNVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTKIMDFSSLSPGTCV*SREAVTWGV 565
GPK + + + G+T+R P I+ + D +T + T + T V
Sbjct: 106 GPKGLAEVARNLGKTLRTFQPTIRELQDVSRDFKSTLEREIGLDDISTPNVYNQNRTNPV 165
Query: 566 W-APSCPARDIPAPSTLCTSRTPRDTPSPRG*TT 664
P P +P+ T+ P D+ SP+ T+
Sbjct: 166 QPPPPPPPPSVPSTEAPVTANDPNDSQSPKAYTS 199
>At4g08180.3 68417.m01353 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 27.5 bits (58), Expect = 8.7
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +3
Query: 141 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 239
N ++A+T NE+ +K+RL D ++R D Y
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 735
>At4g08180.2 68417.m01352 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 27.5 bits (58), Expect = 8.7
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +3
Query: 141 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 239
N ++A+T NE+ +K+RL D ++R D Y
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 735
>At4g08180.1 68417.m01351 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 814
Score = 27.5 bits (58), Expect = 8.7
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +3
Query: 141 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 239
N ++A+T NE+ +K+RL D ++R D Y
Sbjct: 704 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 736
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,724,548
Number of Sequences: 28952
Number of extensions: 375624
Number of successful extensions: 1122
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 1047
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1120
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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