Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV31025
(666 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g27380.1 68418.m03269 glutathione synthetase (GSH2) non-conse... 99 3e-21
At1g63540.1 68414.m07183 hydroxyproline-rich glycoprotein family... 33 0.23
At1g33770.1 68414.m04174 protein kinase family protein contains ... 31 0.69
At5g04050.1 68418.m00386 maturase-related contains similarity to... 28 4.9
At4g30490.1 68417.m04329 AFG1-like ATPase family protein contain... 28 4.9
At4g02110.1 68417.m00282 BRCT domain-containing protein contains... 28 4.9
At1g50030.1 68414.m05614 target of rapamycin protein (TOR) ident... 28 4.9
At4g03080.1 68417.m00416 kelch repeat-containing serine/threonin... 28 6.4
At3g50180.1 68416.m05486 hypothetical protein 28 6.4
At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / sta... 28 6.4
At2g03880.1 68415.m00350 pentatricopeptide (PPR) repeat-containi... 28 6.4
At1g65910.1 68414.m07479 no apical meristem (NAM) family protein... 28 6.4
At4g03550.1 68417.m00486 glycosyl transferase family 48 protein ... 27 8.5
At4g01120.1 68417.m00150 G-box binding factor 2 (GBF2) identical... 27 8.5
At3g55060.1 68416.m06115 expressed protein contains weak similar... 27 8.5
At2g31850.1 68415.m03889 expressed protein 27 8.5
>At5g27380.1 68418.m03269 glutathione synthetase (GSH2)
non-consensus AT donor splice site at exon 6, AC
acceptor splice site at exon 7; identical to
Swiss-Prot:P46416 glutathione synthetase, chloroplast
precursor (Glutathione synthase) (GSH synthetase)
(GSH-S) [Arabidopsis thaliana]
Length = 478
Score = 98.7 bits (235), Expect = 3e-21
Identities = 43/78 (55%), Positives = 58/78 (74%)
Frame = +1
Query: 1 ERELNDKKQLILDGHPVAVVYYRSGYEPAQYPTAREWDARLRVERSTAIKCPSIHYQLAG 180
E + + + LI+ G VAVVY+RSGY P +P+ EW+ARL +E S+A+KCPSI Y L G
Sbjct: 252 EGSVQEDETLIVGGQAVAVVYFRSGYTPNDHPSESEWNARLLIEESSAVKCPSIAYHLTG 311
Query: 181 TKKVQQALAGPGVLEKFM 234
+KK+QQ LA PGVLE+F+
Sbjct: 312 SKKIQQELAKPGVLERFL 329
Score = 78.2 bits (184), Expect = 5e-15
Identities = 40/92 (43%), Positives = 61/92 (66%), Gaps = 2/92 (2%)
Frame = +3
Query: 240 RDEHLRVRDIFTGLYSLDFDESGERAVEMALADAERFVLKPQREGGGNNVYGSEVRDALT 419
+++ ++R F GL+SLD E ++A+E FV+KPQREGGGNN+YG +VR+ L
Sbjct: 332 KEDIAKLRKCFAGLWSLDDSEIVKQAIEKP----GLFVMKPQREGGGNNIYGDDVRENLL 387
Query: 420 RMRHSRE--RAAYILMERILPPLVSGYMIRPG 509
R++ E AAYILM+RI P + + +++R G
Sbjct: 388 RLQKEGEEGNAAYILMQRIFPKVSNMFLVREG 419
Score = 53.2 bits (122), Expect = 1e-07
Identities = 20/42 (47%), Positives = 34/42 (80%)
Frame = +2
Query: 539 VSELGIFGVIIGTKEKIYCNRQVGHMLRTKLADANEGGVAAG 664
+SELG++G + +K+++ N Q G+++RTK+A ++EGGVAAG
Sbjct: 427 ISELGVYGAYLRSKDEVIVNEQSGYLMRTKIASSDEGGVAAG 468
>At1g63540.1 68414.m07183 hydroxyproline-rich glycoprotein family
protein
Length = 635
Score = 32.7 bits (71), Expect = 0.23
Identities = 19/44 (43%), Positives = 26/44 (59%)
Frame = -1
Query: 510 APALSCTLIPEAAISSPSVCTRPAPGCVACESTRPSLPNRTHYS 379
APA + P AA S P+V + PA GC AC +T S +R H++
Sbjct: 533 APASTIGSRPNAAFSPPTV-SPPAHGCTACGATSSSSASR-HFT 574
>At1g33770.1 68414.m04174 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 614
Score = 31.1 bits (67), Expect = 0.69
Identities = 16/54 (29%), Positives = 31/54 (57%)
Frame = +1
Query: 88 QYPTAREWDARLRVERSTAIKCPSIHYQLAGTKKVQQALAGPGVLEKFMGSGTS 249
+YP ++E DA++R E + K ++ ++ G + V++ L V +F+ SG S
Sbjct: 439 KYPPSKELDAKVRDEEAKRKKAEAVKWR--GHESVRRGLRDSKVTPEFIASGNS 490
>At5g04050.1 68418.m00386 maturase-related contains similarity to
maturase proteins from several species
Length = 567
Score = 28.3 bits (60), Expect = 4.9
Identities = 8/18 (44%), Positives = 13/18 (72%)
Frame = +1
Query: 313 EQWRWHWPMPRDSY*SLK 366
E+W+WHW + R + SL+
Sbjct: 451 ERWKWHWLLTRGDFLSLR 468
>At4g30490.1 68417.m04329 AFG1-like ATPase family protein contains
Pfam profile: PF03969 AFG1-like ATPase
Length = 497
Score = 28.3 bits (60), Expect = 4.9
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +1
Query: 292 TSTRAAREQWRWHWPMPRDSY*SLKG 369
TS +++R +W W MP+ SY +KG
Sbjct: 138 TSDKSSRSRWFWSRLMPQTSYSPVKG 163
>At4g02110.1 68417.m00282 BRCT domain-containing protein contains
Pfam domain, PF00533: BRCA1 C Terminus (BRCT) domain
Length = 1293
Score = 28.3 bits (60), Expect = 4.9
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +2
Query: 455 TDGEDIAASGIRVHDKAGAPVPPPLVDLVSEL 550
TDG +RVH + A +PPP L+ EL
Sbjct: 370 TDGSVSRKDSLRVHHNSEASIPPPSSLLLQEL 401
>At1g50030.1 68414.m05614 target of rapamycin protein (TOR) identical
to pTOR [Arabidopsis thaliana] GI:12002902; contains Pfam
profiles PF00454 Phosphatidylinositol 3- and 4-kinase,
PF02259 FAT domain, PF02260 FATC domain
Length = 2481
Score = 28.3 bits (60), Expect = 4.9
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = -2
Query: 311 LAALVEVQRVQSREYIPDSEVLVPEPMNFSSTPGPAR 201
L LV + R R+Y+P+ LV E + + PGP R
Sbjct: 936 LGTLVSIVRQHIRKYLPELLSLVSELWSSFTLPGPIR 972
>At4g03080.1 68417.m00416 kelch repeat-containing serine/threonine
phosphoesterase family protein contains Pfam profiles:
PF00149 calcineurin-like phosphoesterase, PF01344 kelch
motif
Length = 881
Score = 27.9 bits (59), Expect = 6.4
Identities = 14/28 (50%), Positives = 15/28 (53%)
Frame = +2
Query: 215 GCWRSSWAPGRAPPSPGYIHGTVLAGLR 298
G W + APG AP SP Y H V G R
Sbjct: 243 GQWEWTLAPGVAP-SPRYQHAAVFVGAR 269
>At3g50180.1 68416.m05486 hypothetical protein
Length = 588
Score = 27.9 bits (59), Expect = 6.4
Identities = 23/80 (28%), Positives = 32/80 (40%), Gaps = 3/80 (3%)
Frame = +1
Query: 91 YPTAREW--DARLRVERSTAIKCP-SIHYQLAGTKKVQQALAGPGVLEKFMGSGTSTSES 261
Y RE+ + R + E S + P SI Y+ + Q + PG+ E GSGT E
Sbjct: 96 YDKLREYVENVRRQNEESERVVLPKSISYEDQNLENQQDTRSKPGINEVVEGSGTKRLEW 155
Query: 262 GIYSRDCTRWTSTRAAREQW 321
I +D R W
Sbjct: 156 VISIKDKLEQAYRNDDRTSW 175
>At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / starch
branching enzyme class II (SBE2-1) nearly identical to
starch branching enzyme class II [Arabidopsis thaliana]
GI:619939
Length = 858
Score = 27.9 bits (59), Expect = 6.4
Identities = 16/41 (39%), Positives = 22/41 (53%)
Frame = +2
Query: 56 SFTTDQGTSRHSIPPQGNGTRDYASKDPRP*NVHRSTTNWR 178
S + DQ + IPP G+G R Y DP N HR+ ++R
Sbjct: 139 SHSVDQEVGQRKIPPPGDGKRIY-DIDPML-NSHRNHLDYR 177
>At2g03880.1 68415.m00350 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 630
Score = 27.9 bits (59), Expect = 6.4
Identities = 13/57 (22%), Positives = 30/57 (52%)
Frame = -2
Query: 434 AVSHASQRVPHFRTVHIIPATFPLRLQYESLGIGQCHLHCSLAALVEVQRVQSREYI 264
++ H S+++ + +P +R++ G CH+ C LA+ +E++ + R+ I
Sbjct: 558 SLRHHSEKLALAFGLMTLPIEKVIRIRKNLRICGDCHVFCKLASKLEIRSIVIRDPI 614
>At1g65910.1 68414.m07479 no apical meristem (NAM) family protein
similar to jasmonic acid 2 GI:6175246 from [Lycopersicon
esculentum]; similar to NAC2 (GI:6456751) {Arabidopsis
thaliana}
Length = 631
Score = 27.9 bits (59), Expect = 6.4
Identities = 25/71 (35%), Positives = 34/71 (47%), Gaps = 5/71 (7%)
Frame = +2
Query: 467 DIAASGIRVHDKAGAPVPPPLVDLVSELGIFG--VIIGTKE---KIYCNRQVGHMLRTKL 631
DIA R+ ++ PVPP V+ ++ FG V T E KI Q H R L
Sbjct: 258 DIALECARLQNRMLPPVPPLYVEGLTHNEYFGNNVANDTDEMLSKIIALAQASHEPRNSL 317
Query: 632 ADANEGGVAAG 664
D+ +GG A+G
Sbjct: 318 -DSWDGGSASG 327
>At4g03550.1 68417.m00486 glycosyl transferase family 48 protein
contains Pfam profile: PF02364 1,3-beta-glucan synthase
Length = 1780
Score = 27.5 bits (58), Expect = 8.5
Identities = 9/28 (32%), Positives = 17/28 (60%)
Frame = +1
Query: 529 GRPRVGTRNIWGHYWYEREDLLQSSGRS 612
GR + W +WYE +D L+++G++
Sbjct: 1562 GRISTKSEQSWEKWWYEEQDHLRNTGKA 1589
>At4g01120.1 68417.m00150 G-box binding factor 2 (GBF2) identical to
G-box binding factor 2 (GBF2) SP:P42775 from
[Arabidopsis thaliana];contains Pfam profile: PF00170
bZIP transcription factor
Length = 360
Score = 27.5 bits (58), Expect = 8.5
Identities = 16/54 (29%), Positives = 23/54 (42%), Gaps = 1/54 (1%)
Frame = +2
Query: 98 PQGNGTRDYASKDPRP*NVHRSTTNWRERRRSNKPWP-VPGCWRSSWAPGRAPP 256
P N + + P NVH +W + P +P + S+ APG APP
Sbjct: 9 PTNNSDKPSQAAAPEQSNVHVYHHDWAAMQAYYGPRVGIPQYYNSNLAPGHAPP 62
>At3g55060.1 68416.m06115 expressed protein contains weak similarity
to intracellular protein transport protein USO1
(Swiss-Prot:P25386) [Saccharomyces cerevisiae];
expression supported by MPSS
Length = 896
Score = 27.5 bits (58), Expect = 8.5
Identities = 12/43 (27%), Positives = 24/43 (55%)
Frame = +3
Query: 210 SRGAGEVHGLRDEHLRVRDIFTGLYSLDFDESGERAVEMALAD 338
S+ G+ G E + ++D++ G + FD S + A ++LA+
Sbjct: 229 SQTHGKSKGSNHEPITIQDVYGGSLNRTFDSSSDIAANVSLAE 271
>At2g31850.1 68415.m03889 expressed protein
Length = 113
Score = 27.5 bits (58), Expect = 8.5
Identities = 16/38 (42%), Positives = 18/38 (47%)
Frame = +2
Query: 227 SSWAPGRAPPSPGYIHGTVLAGLRRERRESSGDGTGRC 340
SS P AP S T L GLR++ SG TG C
Sbjct: 38 SSAVPTPAPTSAPAFVATRLNGLRQDDAGLSGSSTGSC 75
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,307,622
Number of Sequences: 28952
Number of extensions: 407930
Number of successful extensions: 1339
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1245
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1333
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1403159472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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