Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV31000
(505 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g15810.1 68418.m01850 N2,N2-dimethylguanosine tRNA methyltran... 66 2e-11
At3g02320.1 68416.m00214 N2,N2-dimethylguanosine tRNA methyltran... 65 3e-11
At1g51890.1 68414.m05849 leucine-rich repeat protein kinase, put... 33 0.11
At5g25930.1 68418.m03081 leucine-rich repeat family protein / pr... 31 0.44
At4g17870.1 68417.m02664 expressed protein 28 3.1
At3g61850.2 68416.m06946 Dof zinc finger protein DAG1 / Dof affe... 28 4.1
At3g61850.1 68416.m06945 Dof zinc finger protein DAG1 / Dof affe... 28 4.1
At4g31430.2 68417.m04465 expressed protein 27 5.4
At4g31430.1 68417.m04464 expressed protein 27 5.4
At1g75210.1 68414.m08737 5' nucleotidase family protein contains... 27 5.4
At1g55610.1 68414.m06365 protein kinase family protein contains ... 27 5.4
At5g13710.1 68418.m01596 sterol 24-C-methyltransferase, putative... 27 9.5
>At5g15810.1 68418.m01850 N2,N2-dimethylguanosine tRNA
methyltransferase family protein similar to SP|Q9P804
N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC
2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase)
{Schizosaccharomyces pombe}; contains Pfam profile
PF02005: N2,N2-dimethylguanosine tRNA methyltransferase
Length = 691
Score = 65.7 bits (153), Expect = 2e-11
Identities = 35/88 (39%), Positives = 51/88 (57%), Gaps = 2/88 (2%)
Frame = +3
Query: 18 IMRSAIINAGYKVSYSHASKMSVKTNAPPQLMWDIIRTWEKTHPIKQSKLVSDPVTKHIL 197
+ RSA+INA Y+VS SH + + +KT+AP +++WDI+R W K HPIK + IL
Sbjct: 528 LFRSAVINAKYRVSGSHVNPLGIKTDAPMEIIWDIMRCWVKNHPIKPQS--PEHPGSVIL 585
Query: 198 SQDIKNNVDLS--VNELANPLAAVTAGF 275
S++ + D S V L+ A A F
Sbjct: 586 SKEPSHQADFSRHVGSLSKAQAKKVARF 613
>At3g02320.1 68416.m00214 N2,N2-dimethylguanosine tRNA
methyltransferase family protein similar to
N2,N2-dimethylguanosine tRNA methyltransferase [Homo
sapiens] GI:11066198; contains Pfam profile PF02005:
N2,N2-dimethylguanosine tRNA methyltransferase
Length = 599
Score = 64.9 bits (151), Expect = 3e-11
Identities = 33/88 (37%), Positives = 52/88 (59%), Gaps = 2/88 (2%)
Frame = +3
Query: 18 IMRSAIINAGYKVSYSHASKMSVKTNAPPQLMWDIIRTWEKTHPIKQSKLVSDPVTKHIL 197
+ RSA+INA Y++S +H + + +KT+AP +++WDI+R W K HPIK + IL
Sbjct: 440 MFRSAVINANYRISGTHVNPLGMKTDAPMEVIWDIMRCWVKNHPIKAQS--PEQPGSVIL 497
Query: 198 SQDIKNNVDLS--VNELANPLAAVTAGF 275
S++ + VD S + L+ A A F
Sbjct: 498 SKEPSHEVDFSRHIGSLSKAQAKKVARF 525
>At1g51890.1 68414.m05849 leucine-rich repeat protein kinase,
putative similar to light repressible receptor protein
kinase [Arabidopsis thaliana] gi|1321686|emb|CAA66376;
contains leucine rich repeat (LRR) domains,
Pfam:PF00560; contains protein kinase domain,
Pfam:PF00069
Length = 888
Score = 33.1 bits (72), Expect = 0.11
Identities = 29/126 (23%), Positives = 52/126 (41%), Gaps = 3/126 (2%)
Frame = +3
Query: 27 SAIINAGYKVSYSHASKMSVKTNAPPQLMWDIIRTWEKTHPIKQSKLVSDPVTKHILSQD 206
SA++N S S AP W+++ + S L S +T I
Sbjct: 364 SAMMNIKTIYGLSKRSSWQGDPCAPELYRWEVLTFGLSIRNLSGSNL-SGTITSDISKLT 422
Query: 207 IKNNVDLSVNELANPLAAVTAGFASRSILHRSGDLG---PEPQSISATTKCRSLLKIRTR 377
+DLS N+L+ + V + + ++++ SG+ P+++ +SL IR
Sbjct: 423 HLRELDLSNNDLSGDIPFVFSDMKNLTLINLSGNKNLNRSVPETLQKRIDNKSLTLIRDE 482
Query: 378 TGRKKT 395
TG+ T
Sbjct: 483 TGKNST 488
>At5g25930.1 68418.m03081 leucine-rich repeat family protein /
protein kinase family protein contains similarity to
Swiss-Prot:P47735 receptor-like protein kinase 5
precursor [Arabidopsis thaliana]; contains Pfam domains
PF00560: Leucine Rich Repeat and PF00069: Protein kinase
domain
Length = 1005
Score = 31.1 bits (67), Expect = 0.44
Identities = 25/80 (31%), Positives = 35/80 (43%), Gaps = 2/80 (2%)
Frame = +3
Query: 216 NVDLSVNELANPLAAVTAGFASRS--ILHRSGDLGPEPQSISATTKCRSLLKIRTRTGRK 389
+VDLSVN L + V G + + L +G G P+SISAT L TG
Sbjct: 239 HVDLSVNNLTGRIPDVLFGLKNLTEFYLFANGLTGEIPKSISATNLVFLDLSANNLTGSI 298
Query: 390 KTMLKENIRIQVTMIHGKKL 449
+ ++QV + KL
Sbjct: 299 PVSIGNLTKLQVLNLFNNKL 318
>At4g17870.1 68417.m02664 expressed protein
Length = 191
Score = 28.3 bits (60), Expect = 3.1
Identities = 11/31 (35%), Positives = 20/31 (64%)
Frame = +3
Query: 63 SHASKMSVKTNAPPQLMWDIIRTWEKTHPIK 155
S +S + + +APP+L+W I+R ++K K
Sbjct: 29 SCSSLHAQRIHAPPELVWSIVRRFDKPQTYK 59
>At3g61850.2 68416.m06946 Dof zinc finger protein DAG1 / Dof
affecting germination 1 (DAG1) / transcription factor
BBFa (BBFA) identical to SP|Q43385 DOF zinc finger
protein DAG1 (Dof affecting germination 1)
(Transcription factor BBFa) (AtBBFa) (rolB domain B
factor a) {Arabidopsis thaliana}
Length = 284
Score = 27.9 bits (59), Expect = 4.1
Identities = 10/38 (26%), Positives = 20/38 (52%)
Frame = +2
Query: 53 SILFARIENVSEDERSTTIDVGHHQDVGENTPNQAEQA 166
S+ F + + + + + + HHQ +G NT N ++A
Sbjct: 197 SLGFPTMPDYKQSNNNLSFSIDHHQGIGHNTINSNQRA 234
>At3g61850.1 68416.m06945 Dof zinc finger protein DAG1 / Dof
affecting germination 1 (DAG1) / transcription factor
BBFa (BBFA) identical to SP|Q43385 DOF zinc finger
protein DAG1 (Dof affecting germination 1)
(Transcription factor BBFa) (AtBBFa) (rolB domain B
factor a) {Arabidopsis thaliana}
Length = 296
Score = 27.9 bits (59), Expect = 4.1
Identities = 10/38 (26%), Positives = 20/38 (52%)
Frame = +2
Query: 53 SILFARIENVSEDERSTTIDVGHHQDVGENTPNQAEQA 166
S+ F + + + + + + HHQ +G NT N ++A
Sbjct: 209 SLGFPTMPDYKQSNNNLSFSIDHHQGIGHNTINSNQRA 246
>At4g31430.2 68417.m04465 expressed protein
Length = 574
Score = 27.5 bits (58), Expect = 5.4
Identities = 10/41 (24%), Positives = 26/41 (63%)
Frame = +2
Query: 41 RWL*SILFARIENVSEDERSTTIDVGHHQDVGENTPNQAEQ 163
+++ S++F+ + SE++ ++ D+ +DV +N P+ E+
Sbjct: 68 KFISSVVFSDSSSSSEEDEDSSSDIDGDEDVEKNNPDFTEE 108
>At4g31430.1 68417.m04464 expressed protein
Length = 485
Score = 27.5 bits (58), Expect = 5.4
Identities = 10/41 (24%), Positives = 26/41 (63%)
Frame = +2
Query: 41 RWL*SILFARIENVSEDERSTTIDVGHHQDVGENTPNQAEQ 163
+++ S++F+ + SE++ ++ D+ +DV +N P+ E+
Sbjct: 68 KFISSVVFSDSSSSSEEDEDSSSDIDGDEDVEKNNPDFTEE 108
>At1g75210.1 68414.m08737 5' nucleotidase family protein contains
Pfam profile: PF05761 5' nucleotidase family
Length = 642
Score = 27.5 bits (58), Expect = 5.4
Identities = 21/81 (25%), Positives = 33/81 (40%), Gaps = 1/81 (1%)
Frame = +3
Query: 174 DPVTKHILSQDIKNNVDLSVNELANPLAAVTAGFASRSILHRSGDLGPEP-QSISATTKC 350
D T L + + LS +L+ PL+ + F+ RS H P P + TT
Sbjct: 36 DSYTPQCLFNSLTTTMRLSKRQLSLPLSLCFSTFSPRSRFHHFPGKYPAPRREFLHTTGT 95
Query: 351 RSLLKIRTRTGRKKTMLKENI 413
L + +K + L EN+
Sbjct: 96 SQLCFKSSMEDQKPSALSENV 116
>At1g55610.1 68414.m06365 protein kinase family protein contains
Prosite:PS00107: Protein kinases ATP-binding region
signature
Length = 1166
Score = 27.5 bits (58), Expect = 5.4
Identities = 14/32 (43%), Positives = 18/32 (56%), Gaps = 1/32 (3%)
Frame = -1
Query: 325 CGSGPRSPERCRIDLEAKPAVTAA-SGLASSF 233
CGS PR P RI + + TA +G+A SF
Sbjct: 755 CGSAPRRPITSRIHAKKQTVATAVIAGIAFSF 786
>At5g13710.1 68418.m01596 sterol 24-C-methyltransferase, putative
similar to SP:P25087 Sterol 24-C-methyltransferase,
Delta(24)-sterol C- methyltransferase, Saccharomyces
cerevisiae
Length = 336
Score = 26.6 bits (56), Expect = 9.5
Identities = 14/42 (33%), Positives = 20/42 (47%)
Frame = -2
Query: 333 ILTAALDPGPQNGAGLIWKRSLPSRRPVDWLAHSPTNRHYSL 208
+ T L+ Q G +IW++ L PV W N H+SL
Sbjct: 231 LTTKCLEALKQAGFEVIWEKDLAKDSPVPWYLPLDKN-HFSL 271
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,916,920
Number of Sequences: 28952
Number of extensions: 190948
Number of successful extensions: 623
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 603
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 623
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 898188928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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