Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30998
(449 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 44 4e-05
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 43 1e-04
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 43 1e-04
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 42 1e-04
At4g27670.1 68417.m03979 25.3 kDa small heat shock protein, chlo... 41 4e-04
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 40 0.001
At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein (HS... 39 0.002
At1g52560.1 68414.m05933 26.5 kDa class I small heat shock prote... 33 0.067
At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock pro... 29 1.1
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 29 1.5
At1g76180.1 68414.m08846 dehydrin (ERD14) identical to SP|P42763... 27 4.4
At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase... 27 4.4
At4g02400.1 68417.m00324 U3 ribonucleoprotein (Utp) family prote... 27 5.9
At4g33495.1 68417.m04757 expressed protein 27 7.7
At4g26260.1 68417.m03779 expressed protein similar to myo-inosit... 27 7.7
At2g38410.1 68415.m04718 VHS domain-containing protein / GAT dom... 27 7.7
At1g75540.1 68414.m08779 zinc finger (B-box type) family protein... 27 7.7
At1g51405.1 68414.m05786 myosin-related low similarity to nonmus... 27 7.7
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 44.4 bits (100), Expect = 4e-05
Identities = 25/85 (29%), Positives = 49/85 (57%), Gaps = 8/85 (9%)
Frame = +3
Query: 12 EEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPDTVESRLS 167
EE+ V+ DG ++ + G+ +EE+ D+ + R +FTRR+ LPEN + +++ +
Sbjct: 73 EEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKMEEIKASM- 131
Query: 168 SDGVLTVIAPRTPAATKNERAVPIT 242
+GVL+V P+ P +++ I+
Sbjct: 132 ENGVLSVTVPKVPEKKPEVKSIDIS 156
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 42.7 bits (96), Expect = 1e-04
Identities = 26/85 (30%), Positives = 48/85 (56%), Gaps = 8/85 (9%)
Frame = +3
Query: 12 EEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPDTVESRLS 167
EE+ V+ D V+ + G+ +EE+ D+ + R +F RR+ LPEN + V++ +
Sbjct: 75 EEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKMEEVKATM- 133
Query: 168 SDGVLTVIAPRTPAATKNERAVPIT 242
+GVLTV+ P+ P +++ I+
Sbjct: 134 ENGVLTVVVPKAPEKKPQVKSIDIS 158
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 42.7 bits (96), Expect = 1e-04
Identities = 29/84 (34%), Positives = 45/84 (53%), Gaps = 8/84 (9%)
Frame = +3
Query: 12 EEISVKTADGYVI-VEGKH----EERQDEHGYISR---QFTRRYALPENCNPDTVESRLS 167
EE+ V+ D V+ + G+ EE+QD + R QF+R++ LPEN D V++ +
Sbjct: 71 EEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMDQVKASM- 129
Query: 168 SDGVLTVIAPRTPAATKNERAVPI 239
+GVLTV P+ A K + I
Sbjct: 130 ENGVLTVTVPKVEEAKKKAQVKSI 153
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 42.3 bits (95), Expect = 1e-04
Identities = 27/85 (31%), Positives = 48/85 (56%), Gaps = 8/85 (9%)
Frame = +3
Query: 12 EEISVKTADGYVI-VEG-KHEERQDEHGYISR------QFTRRYALPENCNPDTVESRLS 167
EE+ V+ + V+ + G +H E++D++ R QFTRR+ LPEN D V++ +
Sbjct: 69 EEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMDQVKAAM- 127
Query: 168 SDGVLTVIAPRTPAATKNERAVPIT 242
+GVLTV P+ + +++ I+
Sbjct: 128 ENGVLTVTVPKAETKKADVKSIQIS 152
>At4g27670.1 68417.m03979 25.3 kDa small heat shock protein,
chloroplast precursor (HSP25.3-P) identical to small
heat shock protein, chloroplast precursor SP:P31170 from
[Arabidopsis thaliana]; identified in Scharf, K-D., et
al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 227
Score = 40.7 bits (91), Expect = 4e-04
Identities = 18/74 (24%), Positives = 38/74 (51%), Gaps = 3/74 (4%)
Frame = +3
Query: 6 SPEEISVKTADGYVIVEGKHEERQDEHGYISRQ---FTRRYALPENCNPDTVESRLSSDG 176
S E++ + D ++++G+ ++ + + R + R LP+NC D +++ L +G
Sbjct: 148 SKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVSSYGTRLQLPDNCEKDKIKAEL-KNG 206
Query: 177 VLTVIAPRTPAATK 218
VL + P+T K
Sbjct: 207 VLFITIPKTKVERK 220
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 39.5 bits (88), Expect = 0.001
Identities = 25/85 (29%), Positives = 47/85 (55%), Gaps = 8/85 (9%)
Frame = +3
Query: 12 EEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPDTVESRLS 167
EE+ V+ DG ++ + G+ +EE+ D + R +F RR+ LPEN + V++ +
Sbjct: 72 EEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVEEVKASM- 130
Query: 168 SDGVLTVIAPRTPAATKNERAVPIT 242
+GVL+V P+ + ++V I+
Sbjct: 131 ENGVLSVTVPKVQESKPEVKSVDIS 155
>At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein
(HSP17.6A-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]
Length = 155
Score = 38.7 bits (86), Expect = 0.002
Identities = 27/84 (32%), Positives = 43/84 (51%), Gaps = 8/84 (9%)
Frame = +3
Query: 12 EEISVKTADGYVI-VEGKH----EERQDEHGYISRQ---FTRRYALPENCNPDTVESRLS 167
EE+ V+ D V+ + G+ EE+QD + R F+R++ LPEN D V++ +
Sbjct: 69 EEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGGFSRKFRLPENVKMDQVKASM- 127
Query: 168 SDGVLTVIAPRTPAATKNERAVPI 239
+GVLTV P+ K + I
Sbjct: 128 ENGVLTVTVPKVETNKKKAQVKSI 151
>At1g52560.1 68414.m05933 26.5 kDa class I small heat shock
protein-like (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family; identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 232
Score = 33.5 bits (73), Expect = 0.067
Identities = 19/85 (22%), Positives = 43/85 (50%), Gaps = 9/85 (10%)
Frame = +3
Query: 12 EEISVKTADGYVIVEGKHEERQD-----EHGYISRQ----FTRRYALPENCNPDTVESRL 164
E++ + DG + ++G H+ ++ E Y S + + +LP++ + +++ L
Sbjct: 148 EDVKITVNDGILTIKGDHKAEEEKGSPEEDEYWSSKSYGYYNTSLSLPDDAKVEDIKAEL 207
Query: 165 SSDGVLTVIAPRTPAATKNERAVPI 239
+ GVL ++ PRT KN + + +
Sbjct: 208 KN-GVLNLVIPRTEKPKKNVQEISV 231
>At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock
protein (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family: identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 134
Score = 29.5 bits (63), Expect = 1.1
Identities = 18/63 (28%), Positives = 29/63 (46%)
Frame = +3
Query: 12 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTVI 191
EEI V+ D ++ + F R++ LPE+ + + + DGVLTVI
Sbjct: 49 EEIKVEIEDSIYLIIRTEATPMSPPDQPLKTFKRKFRLPESIDMIGISAGYE-DGVLTVI 107
Query: 192 APR 200
P+
Sbjct: 108 VPK 110
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 29.1 bits (62), Expect = 1.5
Identities = 21/77 (27%), Positives = 29/77 (37%)
Frame = +3
Query: 12 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTVI 191
+EI D Y EG EE ++ R + L E+C + VES D
Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDDDDDDDD 107
Query: 192 APRTPAATKNERAVPIT 242
R KN +PI+
Sbjct: 108 DDRHSRMLKNVTELPIS 124
>At1g76180.1 68414.m08846 dehydrin (ERD14) identical to SP|P42763
Dehydrin ERD14 {Arabidopsis thaliana}
Length = 185
Score = 27.5 bits (58), Expect = 4.4
Identities = 14/41 (34%), Positives = 19/41 (46%)
Frame = +2
Query: 188 NRSQDSGCHEERASCSHHSNRPVRKEIKEPTAEVETNETKQ 310
+RS S S R +KE K+PT EVE E ++
Sbjct: 75 HRSDSSSSSSSEEEGSDGEKRKKKKEKKKPTTEVEVKEEEK 115
>At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase
large subunit 2 (APL2) / ADP-glucose pyrophosphorylase
identical to SP|P55230
Length = 518
Score = 27.5 bits (58), Expect = 4.4
Identities = 15/59 (25%), Positives = 26/59 (44%)
Frame = +3
Query: 12 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTV 188
E+ K + +I+ G H R D ++ + + +C P ESR S G+L +
Sbjct: 198 EDAKTKNVEHVLILSGDHLYRMDYMNFVQKHIESNADITVSCLP-MDESRASDFGLLKI 255
>At4g02400.1 68417.m00324 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 858
Score = 27.1 bits (57), Expect = 5.9
Identities = 16/50 (32%), Positives = 21/50 (42%)
Frame = +3
Query: 27 KTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDG 176
+ D Y EG EE ++ R Y LPE+ + VES DG
Sbjct: 52 ENVDLYEYEEGVPEEESKKNNRYDRVDNYDYELPEDFEDENVESDDDEDG 101
>At4g33495.1 68417.m04757 expressed protein
Length = 409
Score = 26.6 bits (56), Expect = 7.7
Identities = 11/43 (25%), Positives = 24/43 (55%), Gaps = 7/43 (16%)
Frame = -1
Query: 377 RTRNANKINTRYTFLIHSDPRV-------IVLFRWSQLPQWAP 270
RT+ + + R++F+++ P I +++W +LP W+P
Sbjct: 231 RTKGIDAEDVRFSFVVNFPPGFKIGKYFRIAVWKWQRLPYWSP 273
>At4g26260.1 68417.m03779 expressed protein similar to myo-inositol
oxygenase [Sus scrofa] gi|17432544|gb|AAL39076
Length = 317
Score = 26.6 bits (56), Expect = 7.7
Identities = 16/46 (34%), Positives = 22/46 (47%)
Frame = -1
Query: 410 INTIKRSVSQARTRNANKINTRYTFLIHSDPRVIVLFRWSQLPQWA 273
I + +S R N+ T LIH +VI L ++ LPQWA
Sbjct: 126 IQHLLQSAEAIRKDYPNEDWLHLTALIHDLGKVITLPQFGGLPQWA 171
>At2g38410.1 68415.m04718 VHS domain-containing protein / GAT
domain-containing protein weak similarity to hepatocyte
growth factor-regulated tyrosine kinase substrate HRS
isoform 2 [Homo sapiens] GI:9022389; contains Pfam
profiles PF00790: VHS domain, PF03127: GAT domain
Length = 671
Score = 26.6 bits (56), Expect = 7.7
Identities = 15/67 (22%), Positives = 32/67 (47%)
Frame = +3
Query: 6 SPEEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLT 185
S I + G ++ ++EE +DE ++R+ ++ A +P ++ES ++ L
Sbjct: 356 SSSPIPATVSTGKSPIDEEYEEEEDEFAQLARRHSKPPA-SVTTDPTSLESHNAASNALA 414
Query: 186 VIAPRTP 206
+ P P
Sbjct: 415 LALPDPP 421
>At1g75540.1 68414.m08779 zinc finger (B-box type) family protein
similar to zinc finger protein GB:BAA33202 GI:3618312
from [Oryza sativa]
Length = 331
Score = 26.6 bits (56), Expect = 7.7
Identities = 13/51 (25%), Positives = 23/51 (45%)
Frame = +2
Query: 236 HHSNRPVRKEIKEPTAEVETNETKQ*LSDRCESKKCSVC*FCLHSSCVLVR 388
HH+N+ K ++ ++ T L D C+ KK + FC +L +
Sbjct: 33 HHANKLASKHLRFSLLYPSSSNTSSPLCDICQDKKALL--FCQQDRAILCK 81
>At1g51405.1 68414.m05786 myosin-related low similarity to nonmuscle
myosin heavy chain from Gallus gallus [GI:212449],
Xenopus laevis [GI:214624], Homo sapiens [GI:641958]
Length = 487
Score = 26.6 bits (56), Expect = 7.7
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = +2
Query: 212 HEERASCSHHSNRPVRKEIKEPTAEVE 292
HEE+ CS H +RK E AE E
Sbjct: 311 HEEKKDCSRHCKVVMRKIADEVRAEAE 337
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,551,137
Number of Sequences: 28952
Number of extensions: 133952
Number of successful extensions: 534
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 526
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 533
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 732537840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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