Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30994
(674 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 142 4e-35
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 142 4e-35
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 142 4e-35
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 79 6e-16
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 78 1e-15
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 48 9e-07
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 27 1.9
SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces po... 27 2.5
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 27 2.5
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 3.3
SPBC11B10.04c |mrps28||mitochondrial ribosomal protein subunit S... 26 4.3
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 5.7
SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase Cdc... 26 5.7
SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|c... 25 7.6
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 7.6
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 25 10.0
SPAC29B12.03 |spd1||ribonucleotide reductase |Schizosaccharomyce... 25 10.0
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 142 bits (344), Expect = 4e-35
Identities = 65/98 (66%), Positives = 76/98 (77%)
Frame = +3
Query: 228 PSCCCFRAHSGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIQALDAILPPARPTDK 407
P F SG+ GDNM+EP+T MPW++GWQ E K G GK L++A+D+I PPARPTDK
Sbjct: 183 PKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDK 242
Query: 408 LLRLLLQDVYKIGGIGTVPVGRVETGVLKPGTIVSLPP 521
LRL LQDVYKIGGIGTVPVGRVETGV+KPG IV+ P
Sbjct: 243 PLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAP 280
Score = 121 bits (292), Expect = 8e-29
Identities = 58/85 (68%), Positives = 70/85 (82%)
Frame = +1
Query: 1 ADSAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFE 180
AD A+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFE
Sbjct: 109 ADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFE 166
Query: 181 EIKKEVSSYITTIGYNPAAVAFVPI 255
EI KE S++I +G+NP V FVP+
Sbjct: 167 EIVKETSNFIKKVGFNPKTVPFVPV 191
Score = 95.9 bits (228), Expect = 5e-21
Identities = 42/54 (77%), Positives = 48/54 (88%)
Frame = +2
Query: 512 FAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNP 673
FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+P
Sbjct: 278 FAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDP 331
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 142 bits (344), Expect = 4e-35
Identities = 65/98 (66%), Positives = 76/98 (77%)
Frame = +3
Query: 228 PSCCCFRAHSGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIQALDAILPPARPTDK 407
P F SG+ GDNM+EP+T MPW++GWQ E K G GK L++A+D+I PPARPTDK
Sbjct: 183 PKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDK 242
Query: 408 LLRLLLQDVYKIGGIGTVPVGRVETGVLKPGTIVSLPP 521
LRL LQDVYKIGGIGTVPVGRVETGV+KPG IV+ P
Sbjct: 243 PLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAP 280
Score = 122 bits (293), Expect = 6e-29
Identities = 59/85 (69%), Positives = 70/85 (82%)
Frame = +1
Query: 1 ADSAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFE 180
AD AVLI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFE
Sbjct: 109 ADCAVLIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFE 166
Query: 181 EIKKEVSSYITTIGYNPAAVAFVPI 255
EI KE S++I +G+NP V FVP+
Sbjct: 167 EIVKETSNFIKKVGFNPKTVPFVPV 191
Score = 95.9 bits (228), Expect = 5e-21
Identities = 42/54 (77%), Positives = 48/54 (88%)
Frame = +2
Query: 512 FAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNP 673
FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+P
Sbjct: 278 FAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDP 331
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 142 bits (344), Expect = 4e-35
Identities = 65/98 (66%), Positives = 76/98 (77%)
Frame = +3
Query: 228 PSCCCFRAHSGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIQALDAILPPARPTDK 407
P F SG+ GDNM+EP+T MPW++GWQ E K G GK L++A+D+I PPARPTDK
Sbjct: 183 PKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDK 242
Query: 408 LLRLLLQDVYKIGGIGTVPVGRVETGVLKPGTIVSLPP 521
LRL LQDVYKIGGIGTVPVGRVETGV+KPG IV+ P
Sbjct: 243 PLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAP 280
Score = 121 bits (292), Expect = 8e-29
Identities = 58/85 (68%), Positives = 70/85 (82%)
Frame = +1
Query: 1 ADSAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFE 180
AD A+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFE
Sbjct: 109 ADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFE 166
Query: 181 EIKKEVSSYITTIGYNPAAVAFVPI 255
EI KE S++I +G+NP V FVP+
Sbjct: 167 EIVKETSNFIKKVGFNPKTVPFVPV 191
Score = 95.9 bits (228), Expect = 5e-21
Identities = 42/54 (77%), Positives = 48/54 (88%)
Frame = +2
Query: 512 FAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNP 673
FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+P
Sbjct: 278 FAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDP 331
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 79.0 bits (186), Expect = 6e-16
Identities = 38/87 (43%), Positives = 57/87 (65%), Gaps = 2/87 (2%)
Frame = +1
Query: 1 ADSAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFE 180
AD VL+++A GEFEAG + GQTREHA+LA T G+ L+V +NKMD +SE R++
Sbjct: 340 ADIGVLVISARRGEFEAGFERGGQTREHAVLARTQGINHLVVVINKMDEPSVQWSEERYK 399
Query: 181 EIKKEVSSYITTI-GYNPAA-VAFVPI 255
E ++S ++ + GYN V ++P+
Sbjct: 400 ECVDKLSMFLRRVAGYNSKTDVKYMPV 426
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 77.8 bits (183), Expect = 1e-15
Identities = 41/86 (47%), Positives = 55/86 (63%), Gaps = 1/86 (1%)
Frame = +1
Query: 1 ADSAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFE 180
AD AVL+V + FE G +NGQTREHA L LG+ +++V VNK+D +SE RF+
Sbjct: 279 ADFAVLVVDSSQNNFERGFLENGQTREHAYLLRALGISEIVVSVNKLDLMS--WSEDRFQ 336
Query: 181 EIKKEVSSY-ITTIGYNPAAVAFVPI 255
EIK VS + I +G+ + V FVPI
Sbjct: 337 EIKNIVSDFLIKMVGFKTSNVHFVPI 362
Score = 40.7 bits (91), Expect = 2e-04
Identities = 22/46 (47%), Positives = 28/46 (60%)
Frame = +3
Query: 348 GKCLIQALDAILPPARPTDKLLRLLLQDVYKIGGIGTVPVGRVETG 485
G L+ ALD ++PP +P K LRL + DVY+ TV GRVE G
Sbjct: 384 GPTLLSALDQLVPPEKPYRKPLRLSIDDVYRSPRSVTV-TGRVEAG 428
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 48.4 bits (110), Expect = 9e-07
Identities = 27/68 (39%), Positives = 41/68 (60%)
Frame = +1
Query: 4 DSAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEE 183
D A+++V+A G+ QTREH LLA +GVKQ++V +NK+D EP E E
Sbjct: 141 DGAIIVVSATDGQMP-------QTREHLLLARQVGVKQIVVYINKVDMVEPDMIELVEME 193
Query: 184 IKKEVSSY 207
+++ +S Y
Sbjct: 194 MRELLSEY 201
Score = 46.4 bits (105), Expect = 4e-06
Identities = 25/65 (38%), Positives = 38/65 (58%), Gaps = 1/65 (1%)
Frame = +3
Query: 357 LIQALDAILP-PARPTDKLLRLLLQDVYKIGGIGTVPVGRVETGVLKPGTIVSLPPRTSL 533
L++A+D+ + P R TD + ++DV+ I G GTV GRVE G LK G + + S
Sbjct: 234 LMEAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSH 293
Query: 534 LKSNL 548
LK+ +
Sbjct: 294 LKTTV 298
Score = 35.1 bits (77), Expect = 0.009
Identities = 14/43 (32%), Positives = 27/43 (62%)
Frame = +2
Query: 521 ANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 649
+++ T V +EM + L AV GDN G ++++ ++L+RG +
Sbjct: 292 SHLKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMI 334
>SPAC18G6.05c |||translation elongation regulator Gcn1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2670
Score = 27.5 bits (58), Expect = 1.9
Identities = 22/58 (37%), Positives = 27/58 (46%), Gaps = 2/58 (3%)
Frame = -1
Query: 380 DSIESLNEAFSVSFAFFTLHLPSLEPR--HFG*RLQHVVSVPSRMGTKATAAGL*PIV 213
D+ + L EA V F HLPS +PR L V+S PS A A L P+V
Sbjct: 1294 DANDRLREATVVLFGTVAQHLPSNDPRLAVVMDSLLSVLSTPSESVQLAVAVCLPPLV 1351
>SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 361
Score = 27.1 bits (57), Expect = 2.5
Identities = 13/37 (35%), Positives = 17/37 (45%)
Frame = -3
Query: 597 TLSPGTASWRASWCISTDLTSVVMFAGAKTQWYLVST 487
T PGT W SW L + V K ++YL +T
Sbjct: 101 TYRPGTQHWPFSWLFPGSLPASVSNRYIKLEYYLEAT 137
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 27.1 bits (57), Expect = 2.5
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = +1
Query: 226 NPAAVAFVPILDGTETTCWSLQPKCLGSRDGRWSVKKAKLTENASFK 366
+PA + V LD + W++QP C + DG A L++NA K
Sbjct: 139 SPAQITDVLQLDKLKDRLWNVQPTCALTGDGLLE-GLAWLSQNAKLK 184
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 3.3
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -2
Query: 634 QFLDGHVLYVETYIVSRYSFLESFV 560
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPBC11B10.04c |mrps28||mitochondrial ribosomal protein subunit
S28|Schizosaccharomyces pombe|chr 2|||Manual
Length = 288
Score = 26.2 bits (55), Expect = 4.3
Identities = 13/34 (38%), Positives = 17/34 (50%), Gaps = 2/34 (5%)
Frame = -3
Query: 528 MFAGAKTQWYLVSTHQFQLC--RRARYQYHRFCI 433
+F GA W L ST+ Q C + Q+H CI
Sbjct: 3 LFEGAFQPWKLASTNLMQQCLLLNKKSQFHTTCI 36
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.8 bits (54), Expect = 5.7
Identities = 10/39 (25%), Positives = 22/39 (56%)
Frame = -2
Query: 658 VTSNITTTQFLDGHVLYVETYIVSRYSFLESFVVHLHRF 542
+T ++ + L H+ +++ SR +FL ++H+H F
Sbjct: 1012 LTQSLQSFSHLSNHIEVLDSTRQSRLTFLCHLILHMHGF 1050
>SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase
Cdc7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1062
Score = 25.8 bits (54), Expect = 5.7
Identities = 14/43 (32%), Positives = 24/43 (55%)
Frame = +1
Query: 283 SLQPKCLGSRDGRWSVKKAKLTENASFKLSMLSCHLPAPLISS 411
SL+ K +GS+D S + L+E+ S K ++ H PL+ +
Sbjct: 528 SLRDKNIGSKDTTVSQIASILSEDLSLKREIIQAHGILPLLET 570
>SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 367
Score = 25.4 bits (53), Expect = 7.6
Identities = 9/30 (30%), Positives = 18/30 (60%)
Frame = +2
Query: 161 TVSPDLRKSRRKSPHTSRRLATTQLLLLSC 250
+ +PD+ + PHT +R +T + L++ C
Sbjct: 145 STNPDVLYMSSQHPHTQQRYSTLKRLMVRC 174
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 7.6
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -2
Query: 490 NTPVSTLPTGTVPIPPIL 437
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 25.0 bits (52), Expect = 10.0
Identities = 12/47 (25%), Positives = 22/47 (46%)
Frame = +1
Query: 70 QTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYI 210
QT EH + +K +I+ NK+D +E ++ I K + +
Sbjct: 149 QTSEHLAAIEIMQLKHIIILQNKVDLIRESAAEEHYQSILKFIKGTV 195
>SPAC29B12.03 |spd1||ribonucleotide reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 124
Score = 25.0 bits (52), Expect = 10.0
Identities = 9/40 (22%), Positives = 17/40 (42%)
Frame = +1
Query: 112 KQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYITTIGYNP 231
K + G +T P Y+ P + + + ++ T Y P
Sbjct: 42 KSISTGYKSKQTTFPAYNPPLYNTVSENIALKNTAFSYEP 81
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,989,626
Number of Sequences: 5004
Number of extensions: 65614
Number of successful extensions: 230
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 209
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 226
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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