Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30983
(785 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g78900.1 68414.m09198 vacuolar ATP synthase catalytic subunit... 131 6e-31
At1g16820.1 68414.m02021 vacuolar ATP synthase catalytic subunit... 42 5e-04
At1g67870.1 68414.m07750 glycine-rich protein contains non-conse... 34 0.12
At1g74210.1 68414.m08595 glycerophosphoryl diester phosphodieste... 31 0.87
At1g48405.1 68414.m05407 hypothetical protein 31 1.1
At5g02600.2 68418.m00195 heavy-metal-associated domain-containin... 30 2.0
At5g02600.1 68418.m00196 heavy-metal-associated domain-containin... 30 2.0
At3g20970.1 68416.m02650 nitrogen fixation NifU-like family prot... 30 2.0
At5g23200.1 68418.m02713 expressed protein 28 8.1
At5g12850.1 68418.m01475 zinc finger (CCCH-type) family protein ... 28 8.1
At4g13310.2 68417.m02080 cytochrome P450 71A20, putative (CYP71A... 28 8.1
At4g13310.1 68417.m02081 cytochrome P450 71A20, putative (CYP71A... 28 8.1
>At1g78900.1 68414.m09198 vacuolar ATP synthase catalytic subunit A
/ V-ATPase A subunit / vacuolar proton pump alpha
subunit / V-ATPase 69 kDa subunit identical to SP|O23654
Vacuolar ATP synthase catalytic subunit A (EC 3.6.3.14)
(V-ATPase A subunit) (Vacuolar proton pump alpha
subunit) (V-ATPase 69 kDa subunit) {Arabidopsis
thaliana}
Length = 623
Score = 131 bits (316), Expect = 6e-31
Identities = 59/86 (68%), Positives = 73/86 (84%)
Frame = +1
Query: 1 VTAATLGIVQVFWGLDKKLAHGEHFPSINWLISYSKYMRALDDFYEKNYPEFVPLRTKVK 180
VT+ATL IVQVFWGLDKKLA +HFPS+NWLISYSKY AL+ FYEK P+F+ +RTK +
Sbjct: 424 VTSATLSIVQVFWGLDKKLAQRKHFPSVNWLISYSKYSTALESFYEKFDPDFINIRTKAR 483
Query: 181 EILQEEEDLSEIVQLVGKASLAETER 258
E+LQ E+DL+EIVQLVGK +LAE ++
Sbjct: 484 EVLQREDDLNEIVQLVGKDALAEGDK 509
Score = 104 bits (249), Expect = 8e-23
Identities = 46/97 (47%), Positives = 72/97 (74%), Gaps = 1/97 (1%)
Frame = +3
Query: 225 GRQGVPRGN*KITLEVAKLLKDDFLQQNSYSSYDRFCPFYKTVGMLKNIITFYDMSRHAV 404
G+ + G+ KITLE AKLL++D+L QN+++ YD+FCPFYK+V M++NII FY+++ AV
Sbjct: 500 GKDALAEGD-KITLETAKLLREDYLAQNAFTPYDKFCPFYKSVWMMRNIIHFYNLANQAV 558
Query: 405 ESTAQSD-NKVTWNVIRDAMGNVLYQLSSMKFKDPVK 512
E A D K+T+ +I+ +G++ Y+L S KF+DP +
Sbjct: 559 ERAAGMDGQKITYTLIKHRLGDLFYRLVSQKFEDPAE 595
>At1g16820.1 68414.m02021 vacuolar ATP synthase catalytic
subunit-related / V-ATPase-related / vacuolar proton
pump-related similar to Vacuolar ATP synthase catalytic
subunit A (V-ATPase A subunit) (Vacuolar proton pump
alpha subunit) (V-ATPase 69 kDa subunit) (SP:O23654)
[Arabidopsis thaliana]
Length = 93
Score = 41.9 bits (94), Expect = 5e-04
Identities = 16/37 (43%), Positives = 27/37 (72%)
Frame = +3
Query: 315 SSYDRFCPFYKTVGMLKNIITFYDMSRHAVESTAQSD 425
++YD+F P YK+V M++NII FY+++ A++ A D
Sbjct: 32 TNYDKFSPSYKSVWMMRNIIHFYNLANQAIDRAAGVD 68
>At1g67870.1 68414.m07750 glycine-rich protein contains
non-consensus GG donor splice site at exon2; modeled to
est match.
Length = 279
Score = 33.9 bits (74), Expect = 0.12
Identities = 17/46 (36%), Positives = 23/46 (50%), Gaps = 6/46 (13%)
Frame = +2
Query: 350 GGHAEEHH--HVLRHVAARG----GVHGAVRQQGHVERHPRRHGQR 469
GGH +HH H ++H G G+HG Q GH +H HG +
Sbjct: 132 GGHGIQHHDIHGMQHQGGHGMQHQGMHGMQHQGGHGMQHQGGHGMQ 177
Score = 30.3 bits (65), Expect = 1.5
Identities = 17/46 (36%), Positives = 21/46 (45%), Gaps = 6/46 (13%)
Frame = +2
Query: 350 GGHAEEHH--HVLRHVAARG----GVHGAVRQQGHVERHPRRHGQR 469
GGH EH H ++H G G HG Q GH +H HG +
Sbjct: 188 GGHGMEHQGGHGMQHQDMHGMQHQGRHGMQHQGGHEMQHQGMHGMQ 233
Score = 29.5 bits (63), Expect = 2.6
Identities = 16/46 (34%), Positives = 21/46 (45%), Gaps = 6/46 (13%)
Frame = +2
Query: 350 GGHAEEHH--HVLRHVAARG----GVHGAVRQQGHVERHPRRHGQR 469
GGH +H H ++H G G HG Q GH +H HG +
Sbjct: 164 GGHGMQHQGGHGMQHQGMHGMQHQGGHGMEHQGGHGMQHQDMHGMQ 209
Score = 28.7 bits (61), Expect = 4.6
Identities = 14/40 (35%), Positives = 18/40 (45%)
Frame = +2
Query: 350 GGHAEEHHHVLRHVAARGGVHGAVRQQGHVERHPRRHGQR 469
GGH +H + H G HG Q GH +H HG +
Sbjct: 148 GGHGMQHQGM--HGMQHQGGHGMQHQGGHGMQHQGMHGMQ 185
>At1g74210.1 68414.m08595 glycerophosphoryl diester
phosphodiesterase family protein low similarity to
glycerophosphodiester phosphodiesterase [Borrelia
hermsii] GI:1399038; contains Pfam profile PF03009:
Glycerophosphoryl diester phosphodiesterase family
Length = 392
Score = 31.1 bits (67), Expect = 0.87
Identities = 17/31 (54%), Positives = 21/31 (67%)
Frame = -2
Query: 541 EIGLDLRFTVFTGSLNFMEESW*STLPMASR 449
EIG+D FT FTGSL+ +E W S LP S+
Sbjct: 344 EIGVDGLFTDFTGSLHNFQE-WTSPLPDTSK 373
>At1g48405.1 68414.m05407 hypothetical protein
Length = 297
Score = 30.7 bits (66), Expect = 1.1
Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 1/32 (3%)
Frame = +3
Query: 345 KTVGMLKNIITFYDMSRH-AVESTAQSDNKVT 437
KT+G+LK ++ DM + AVE TA +K+T
Sbjct: 145 KTIGLLKKLVMLLDMEKEVAVEETANLGHKLT 176
>At5g02600.2 68418.m00195 heavy-metal-associated domain-containing
protein low similarity to gi:3168840 copper homeostasis
factor; contains Pfam heavy-metal-associated domain
PF00403; predicted proteins, Arabidopsis thaliana
Length = 319
Score = 29.9 bits (64), Expect = 2.0
Identities = 18/62 (29%), Positives = 27/62 (43%)
Frame = +1
Query: 91 LISYSKYMRALDDFYEKNYPEFVPLRTKVKEILQEEEDLSEIVQLVGKASLAETERSRSR 270
+I+ SKY+ F EK P+F + +L + SE + ASL+ E
Sbjct: 158 IINASKYLSTSASFLEKKQPDFFEGFLDYEPVLSPDNPFSEPTKASPTASLSSLEDKDVS 217
Query: 271 SP 276
SP
Sbjct: 218 SP 219
>At5g02600.1 68418.m00196 heavy-metal-associated domain-containing
protein low similarity to gi:3168840 copper homeostasis
factor; contains Pfam heavy-metal-associated domain
PF00403; predicted proteins, Arabidopsis thaliana
Length = 319
Score = 29.9 bits (64), Expect = 2.0
Identities = 18/62 (29%), Positives = 27/62 (43%)
Frame = +1
Query: 91 LISYSKYMRALDDFYEKNYPEFVPLRTKVKEILQEEEDLSEIVQLVGKASLAETERSRSR 270
+I+ SKY+ F EK P+F + +L + SE + ASL+ E
Sbjct: 158 IINASKYLSTSASFLEKKQPDFFEGFLDYEPVLSPDNPFSEPTKASPTASLSSLEDKDVS 217
Query: 271 SP 276
SP
Sbjct: 218 SP 219
>At3g20970.1 68416.m02650 nitrogen fixation NifU-like family protein
simikar ti HIRA-interacting protein 5 (NIFU FAMILY)
(SP:Q9QZ23) {Mus musculus}; contains Pfam profile:
PF01106 NifU-like domain
Length = 283
Score = 29.9 bits (64), Expect = 2.0
Identities = 19/70 (27%), Positives = 31/70 (44%)
Frame = +1
Query: 19 GIVQVFWGLDKKLAHGEHFPSINWLISYSKYMRALDDFYEKNYPEFVPLRTKVKEILQEE 198
G+V+VF+G D ++W I + A+ DFY P F+ + +
Sbjct: 129 GVVRVFFGSD--FVTVTKSDDVSWDILKPEIFAAVMDFYSSGQPLFLDSQAAAAKDTAIS 186
Query: 199 EDLSEIVQLV 228
ED SE V ++
Sbjct: 187 EDDSETVAMI 196
>At5g23200.1 68418.m02713 expressed protein
Length = 399
Score = 27.9 bits (59), Expect = 8.1
Identities = 10/22 (45%), Positives = 17/22 (77%)
Frame = +1
Query: 193 EEEDLSEIVQLVGKASLAETER 258
EE D EI+++ GKA+L+E ++
Sbjct: 51 EETDAEEIIEMAGKATLSEQQK 72
>At5g12850.1 68418.m01475 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar) and Pfam domain,
PF00023: Ankyrin repeat
Length = 706
Score = 27.9 bits (59), Expect = 8.1
Identities = 17/43 (39%), Positives = 26/43 (60%)
Frame = +1
Query: 148 PEFVPLRTKVKEILQEEEDLSEIVQLVGKASLAETERSRSRSP 276
P LRT ++EIL+++E +SE + +SL + RS S SP
Sbjct: 166 PHAPGLRTILEEILKKDEIISEDLH-ASSSSLGSSFRSLSSSP 207
>At4g13310.2 68417.m02080 cytochrome P450 71A20, putative (CYP71A20)
Identical to Cytochrome P450 (SP:Q9T0K2) [Arabidopsis
thaliana]; similar to cytochrome P450 71A4, Solanum
melongena, PIR2:S36805
Length = 390
Score = 27.9 bits (59), Expect = 8.1
Identities = 25/83 (30%), Positives = 40/83 (48%), Gaps = 3/83 (3%)
Frame = +1
Query: 22 IVQVFWGLDKKLAHGEHFPSINWLISYSKYMRALD---DFYEKNYPEFVPLRTKVKEILQ 192
IV+ F L + GE+ PS++W+ +R LD + +K + EF L VKE
Sbjct: 206 IVRTFAALLGEFPVGEYIPSLSWI----DRIRGLDHKMEVVDKRFDEF--LERVVKE--H 257
Query: 193 EEEDLSEIVQLVGKASLAETERS 261
EE D LV K +++++
Sbjct: 258 EEADKETRSDLVDKLLTIQSDKT 280
>At4g13310.1 68417.m02081 cytochrome P450 71A20, putative (CYP71A20)
Identical to Cytochrome P450 (SP:Q9T0K2) [Arabidopsis
thaliana]; similar to cytochrome P450 71A4, Solanum
melongena, PIR2:S36805
Length = 497
Score = 27.9 bits (59), Expect = 8.1
Identities = 25/83 (30%), Positives = 40/83 (48%), Gaps = 3/83 (3%)
Frame = +1
Query: 22 IVQVFWGLDKKLAHGEHFPSINWLISYSKYMRALD---DFYEKNYPEFVPLRTKVKEILQ 192
IV+ F L + GE+ PS++W+ +R LD + +K + EF L VKE
Sbjct: 206 IVRTFAALLGEFPVGEYIPSLSWI----DRIRGLDHKMEVVDKRFDEF--LERVVKE--H 257
Query: 193 EEEDLSEIVQLVGKASLAETERS 261
EE D LV K +++++
Sbjct: 258 EEADKETRSDLVDKLLTIQSDKT 280
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,120,644
Number of Sequences: 28952
Number of extensions: 246542
Number of successful extensions: 844
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 814
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 840
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1765546400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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