BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV30952X (473 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr 1|||... 30 0.16 SPBC1105.13c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 27 1.5 SPAC23H4.10c |thi4||thiamine-phosphate dipyrophosphorylase/hydro... 27 1.5 SPCC1739.01 ||SPCC1906.05|zf-CCCH type zinc finger protein|Schiz... 26 2.5 SPCC1259.03 |rpa12||DNA-directed RNA polymerase complex I subuni... 26 3.4 SPBC9B6.06 |mrpl10||mitochondrial ribosomal protein subunit L15|... 22 4.9 >SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 504 Score = 30.3 bits (65), Expect = 0.16 Identities = 18/55 (32%), Positives = 25/55 (45%), Gaps = 2/55 (3%) Frame = -2 Query: 463 HCLLPKQLHEHTD--AWSSCERCRAAMPCPSIGSNVLEPSLSSKMSTTTCNCNSR 305 H LL + + D W C A+ C +V + SLSS + T TCNC + Sbjct: 200 HRLLDRSFVDDNDHLRWCPAPDCEFAIEC-----HVTQASLSSVVPTVTCNCGKQ 249 >SPBC1105.13c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 142 Score = 27.1 bits (57), Expect = 1.5 Identities = 16/53 (30%), Positives = 23/53 (43%), Gaps = 2/53 (3%) Frame = -2 Query: 466 SHCLL--PKQLHEHTDAWSSCERCRAAMPCPSIGSNVLEPSLSSKMSTTTCNC 314 + C L PK H+H WS E C A+ G + L S+ + C+C Sbjct: 58 ARCFLHNPKISHQHHSFWSLIEGCLEAL---QFGLLAILQGLFSQFAWEACSC 107 >SPAC23H4.10c |thi4||thiamine-phosphate dipyrophosphorylase/hydroxyethylthiazole kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 518 Score = 27.1 bits (57), Expect = 1.5 Identities = 15/36 (41%), Positives = 19/36 (52%) Frame = -2 Query: 163 TPRETARPLDTMVKSPVFLTSTRGSLHLSKRPKTLL 56 TPRETA+ L ++ +P R SL PK LL Sbjct: 207 TPRETAKELRNLIATPPCFAQARSSL---TTPKDLL 239 >SPCC1739.01 ||SPCC1906.05|zf-CCCH type zinc finger protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 547 Score = 26.2 bits (55), Expect = 2.5 Identities = 22/73 (30%), Positives = 32/73 (43%), Gaps = 6/73 (8%) Frame = -2 Query: 469 LSHCL-----LPKQLHEHTDAWSSCERCRAAMPCPSIGSNVLEPSLSSK-MSTTTCNCNS 308 LSH L LP +T A + +++ +G+N + PSLSSK M N+ Sbjct: 90 LSHALPGNTNLPNGTSTNTMASMAANGGASSVASKQMGANQISPSLSSKTMKNPADKANN 149 Query: 307 RDRVVYGGNSADS 269 GN+A S Sbjct: 150 TTATDVRGNTATS 162 >SPCC1259.03 |rpa12||DNA-directed RNA polymerase complex I subunit Rpa12|Schizosaccharomyces pombe|chr 3|||Manual Length = 119 Score = 25.8 bits (54), Expect = 3.4 Identities = 9/34 (26%), Positives = 18/34 (52%) Frame = -2 Query: 442 LHEHTDAWSSCERCRAAMPCPSIGSNVLEPSLSS 341 L T W++C++C++ P + V+E S+ Sbjct: 17 LESTTAQWTTCDQCQSVYPSEQFANLVVETKSSA 50 >SPBC9B6.06 |mrpl10||mitochondrial ribosomal protein subunit L15|Schizosaccharomyces pombe|chr 2|||Manual Length = 220 Score = 22.2 bits (45), Expect(2) = 4.9 Identities = 7/9 (77%), Positives = 8/9 (88%) Frame = +1 Query: 277 RCCRRIQHG 303 RCCR I+HG Sbjct: 104 RCCRGIKHG 112 Score = 21.4 bits (43), Expect(2) = 4.9 Identities = 10/28 (35%), Positives = 15/28 (53%) Frame = +1 Query: 385 TASRPGSVHTTTRRLYARATVWAISSVR 468 TA PG + T ++ATV AI ++ Sbjct: 116 TADEPGLLKTAISIEVSKATVQAIQQIK 143 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,668,140 Number of Sequences: 5004 Number of extensions: 28276 Number of successful extensions: 82 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 80 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 82 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 182448900 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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