Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30938X
(549 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 142 4e-35
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 57 1e-09
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 57 1e-09
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 57 1e-09
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 56 3e-09
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 54 1e-08
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 50 2e-07
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 41 1e-04
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 40 2e-04
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 40 2e-04
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 33 0.028
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 31 0.085
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 29 0.34
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 27 1.4
SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pm... 27 2.4
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 26 3.2
SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+, L-... 25 5.6
SPAC767.01c |vps1|SPAC9G1.14c|dynamin family protein Vps1|Schizo... 25 5.6
SPMIT.06 |||mitochondrial DNA binding endonuclease|Schizosacchar... 25 5.6
SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr 1... 25 7.3
SPBP22H7.09c |mis15||kinetochore protein Mis15 |Schizosaccharomy... 25 9.7
SPBC4F6.11c |||asparagine synthase |Schizosaccharomyces pombe|ch... 25 9.7
SPBP16F5.06 |||ribosome biogenesis protein Nop6|Schizosaccharomy... 25 9.7
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 142 bits (343), Expect = 4e-35
Identities = 65/84 (77%), Positives = 68/84 (80%)
Frame = +1
Query: 256 IGHVDHGKTTLTAAITKVLSDLNLAQKKGYADIDNAPEEKARGITINVAHVEYQTEQRHY 435
IGHVDHGKTTLTAAITK LSDL A Y+ ID APEEKARGITI+ AHVEY+T RHY
Sbjct: 59 IGHVDHGKTTLTAAITKCLSDLGQASFMDYSQIDKAPEEKARGITISSAHVEYETANRHY 118
Query: 436 GHTDCPGHADYIKNMITGTAQMDG 507
H DCPGHADYIKNMITG A MDG
Sbjct: 119 AHVDCPGHADYIKNMITGAATMDG 142
Score = 34.7 bits (76), Expect = 0.009
Identities = 14/43 (32%), Positives = 23/43 (53%)
Frame = +2
Query: 125 QSQCANVRNTTVLSGLTPLSIILKRHYAEKQIFERTKPHCNVG 253
+ C + + SGL + R YA++++F R KPH N+G
Sbjct: 15 RKNCLRLNRISFASGLINRFTVPARTYADEKVFVRKKPHVNIG 57
Score = 25.0 bits (52), Expect = 7.3
Identities = 11/19 (57%), Positives = 14/19 (73%)
Frame = +3
Query: 492 STNGWCILVVAATDGVMPQ 548
+T I+VV+ATDG MPQ
Sbjct: 138 ATMDGAIIVVSATDGQMPQ 156
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 57.2 bits (132), Expect = 1e-09
Identities = 36/98 (36%), Positives = 51/98 (52%), Gaps = 15/98 (15%)
Frame = +1
Query: 256 IGHVDHGKTTLTAAIT---------------KVLSDLNLAQKKGYADIDNAPEEKARGIT 390
IGHVD GK+T T + K ++L K +D E+ RGIT
Sbjct: 13 IGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERERGIT 72
Query: 391 INVAHVEYQTEQRHYGHTDCPGHADYIKNMITGTAQMD 504
I++A +++T + + D PGH D+IKNMITGT+Q D
Sbjct: 73 IDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQAD 110
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 57.2 bits (132), Expect = 1e-09
Identities = 36/98 (36%), Positives = 51/98 (52%), Gaps = 15/98 (15%)
Frame = +1
Query: 256 IGHVDHGKTTLTAAIT---------------KVLSDLNLAQKKGYADIDNAPEEKARGIT 390
IGHVD GK+T T + K ++L K +D E+ RGIT
Sbjct: 13 IGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERERGIT 72
Query: 391 INVAHVEYQTEQRHYGHTDCPGHADYIKNMITGTAQMD 504
I++A +++T + + D PGH D+IKNMITGT+Q D
Sbjct: 73 IDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQAD 110
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 57.2 bits (132), Expect = 1e-09
Identities = 36/98 (36%), Positives = 51/98 (52%), Gaps = 15/98 (15%)
Frame = +1
Query: 256 IGHVDHGKTTLTAAIT---------------KVLSDLNLAQKKGYADIDNAPEEKARGIT 390
IGHVD GK+T T + K ++L K +D E+ RGIT
Sbjct: 13 IGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERERGIT 72
Query: 391 INVAHVEYQTEQRHYGHTDCPGHADYIKNMITGTAQMD 504
I++A +++T + + D PGH D+IKNMITGT+Q D
Sbjct: 73 IDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQAD 110
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 56.0 bits (129), Expect = 3e-09
Identities = 36/97 (37%), Positives = 53/97 (54%), Gaps = 15/97 (15%)
Frame = +1
Query: 259 GHVDHGKTTLTAAITKVLSDLN--LAQK---------KG---YA-DIDNAPEEKARGITI 393
GHVD GK+T+ I L ++N QK KG YA +D EE+ARG+T+
Sbjct: 184 GHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTEEERARGVTM 243
Query: 394 NVAHVEYQTEQRHYGHTDCPGHADYIKNMITGTAQMD 504
+VA ++++++ Y D PGH D+I MI G + D
Sbjct: 244 DVASTTFESDKKIYEIGDAPGHRDFISGMIAGASSAD 280
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 54.4 bits (125), Expect = 1e-08
Identities = 33/98 (33%), Positives = 48/98 (48%), Gaps = 15/98 (15%)
Frame = +1
Query: 256 IGHVDHGKTTLTAAIT------------KVLSDLNLAQKKGYA---DIDNAPEEKARGIT 390
IGHVD GK+TL I K+ + A K+ + +D+ EE+ +G T
Sbjct: 244 IGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTSEEREKGKT 303
Query: 391 INVAHVEYQTEQRHYGHTDCPGHADYIKNMITGTAQMD 504
+ V ++TE R + D PGH Y+ NMI G +Q D
Sbjct: 304 VEVGRAYFETEHRRFSLLDAPGHKGYVTNMINGASQAD 341
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 50.0 bits (114), Expect = 2e-07
Identities = 32/89 (35%), Positives = 42/89 (47%), Gaps = 5/89 (5%)
Frame = +1
Query: 256 IGHVDHGKTTLTAAITKVLSDLNLAQKKGYAD-----IDNAPEEKARGITINVAHVEYQT 420
I H+D GKTTLT K+L G D +D P E+ RGITIN A + +
Sbjct: 34 IAHIDAGKTTLTE---KMLYYGGFTSHFGNVDTGDTVMDYLPAERQRGITINSAAISFTW 90
Query: 421 EQRHYGHTDCPGHADYIKNMITGTAQMDG 507
+ D PGHAD+ + A +DG
Sbjct: 91 RNQRINLIDTPGHADFTFEVERSVAVLDG 119
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 41.1 bits (92), Expect = 1e-04
Identities = 26/88 (29%), Positives = 42/88 (47%), Gaps = 4/88 (4%)
Frame = +1
Query: 256 IGHVDHGKTTLTAAITKVLSDLNLAQKKGYADIDNAPEEKARGITINV---AHVEYQTEQ 426
I H+DHGK+TL+ I K+ +N + +D E+ RGIT+ + + Y Q
Sbjct: 64 IAHIDHGKSTLSDCILKLTGVINEHNFRNQF-LDKLEVERRRGITVKAQTCSMIYYYHGQ 122
Query: 427 RH-YGHTDCPGHADYIKNMITGTAQMDG 507
+ D PGH D+ ++ A +G
Sbjct: 123 SYLLNLIDTPGHVDFRAEVMHSLAACEG 150
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 40.3 bits (90), Expect = 2e-04
Identities = 24/86 (27%), Positives = 40/86 (46%), Gaps = 3/86 (3%)
Frame = +1
Query: 256 IGHVDHGKTTLTAAITKVLSDLNLAQKKGYADIDNAPEEKARGITINVAHVEYQTEQRHY 435
I HVDHGK+TLT ++ + ++ A+ +D +E+ RG+TI + E
Sbjct: 25 IAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQERGVTIKSTAISLFAEMTDD 84
Query: 436 GHTDCPGHA---DYIKNMITGTAQMD 504
D A D++ N+I +D
Sbjct: 85 DMKDMKEPADGTDFLVNLIDSPGHVD 110
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 40.3 bits (90), Expect = 2e-04
Identities = 24/86 (27%), Positives = 40/86 (46%), Gaps = 3/86 (3%)
Frame = +1
Query: 256 IGHVDHGKTTLTAAITKVLSDLNLAQKKGYADIDNAPEEKARGITINVAHVEYQTEQRHY 435
I HVDHGK+TLT ++ + ++ A+ +D +E+ RG+TI + E
Sbjct: 25 IAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQERGVTIKSTAISLFAEMTDD 84
Query: 436 GHTDCPGHA---DYIKNMITGTAQMD 504
D A D++ N+I +D
Sbjct: 85 DMKDMKEPADGTDFLVNLIDSPGHVD 110
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 33.1 bits (72), Expect = 0.028
Identities = 34/113 (30%), Positives = 48/113 (42%), Gaps = 22/113 (19%)
Frame = +1
Query: 232 KAPLQCWQIGHVDHGKTTLTAAITKVLS-------DLNLAQKKGYAD--IDNAPEEKA-- 378
+A + IGHV HGK+T+ AI+ V + + N+ K GYA+ I E+
Sbjct: 21 QATINIGTIGHVAHGKSTVVKAISGVHTVRFKNELERNITIKLGYANAKIYKCSNEECPR 80
Query: 379 ----RGITINVA-HVEYQTEQ------RHYGHTDCPGHADYIKNMITGTAQMD 504
R + N H + RH DCPGH + M+ G A MD
Sbjct: 81 PGCYRSYSSNKEDHPPCEICNSPMNLVRHVSFVDCPGHDILMATMLNGAAVMD 133
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 31.5 bits (68), Expect = 0.085
Identities = 23/98 (23%), Positives = 41/98 (41%), Gaps = 12/98 (12%)
Frame = +1
Query: 256 IGHVDHGKTTLTAAITKVLSDLNLAQKKGYADIDNAPEEKARGITINVAHVEY------Q 417
+ HVDHGKTTL ++ ++ +D +E RGIT+ + + Q
Sbjct: 25 LAHVDHGKTTLADSLLASNGIISSKLAGTVRFLDFREDEITRGITMKSSAISLFFKVISQ 84
Query: 418 TEQRH------YGHTDCPGHADYIKNMITGTAQMDGVY 513
+++ D PGH D+ + + + DG +
Sbjct: 85 NDEKRVEKDYLINLIDSPGHVDFSSEVSSASRLCDGAF 122
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 29.5 bits (63), Expect = 0.34
Identities = 12/17 (70%), Positives = 13/17 (76%)
Frame = +1
Query: 256 IGHVDHGKTTLTAAITK 306
+GHVDHGKTTL A K
Sbjct: 177 MGHVDHGKTTLLDAFRK 193
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 27.5 bits (58), Expect = 1.4
Identities = 21/90 (23%), Positives = 37/90 (41%), Gaps = 6/90 (6%)
Frame = +1
Query: 259 GHVDHGKTTLTAAITKVLS-DLNLAQKKG--YADIDNAPEEKARGIT---INVAHVEYQT 420
GH+ HGK+ L + D +++ Y D E+ I + +A + +
Sbjct: 147 GHLHHGKSALLDLLVYYTHPDTKPPKRRSLRYTDTHYLERERVMSIKSTPLTLAVSDMKG 206
Query: 421 EQRHYGHTDCPGHADYIKNMITGTAQMDGV 510
+ + D PGH D++ + A DGV
Sbjct: 207 KTFAFQCIDTPGHVDFVDEVAAPMAISDGV 236
>SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pmc1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1292
Score = 26.6 bits (56), Expect = 2.4
Identities = 11/18 (61%), Positives = 12/18 (66%)
Frame = +1
Query: 445 DCPGHADYIKNMITGTAQ 498
D HADY K MITG A+
Sbjct: 715 DMHSHADYFKEMITGYAK 732
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 26.2 bits (55), Expect = 3.2
Identities = 18/52 (34%), Positives = 27/52 (51%), Gaps = 5/52 (9%)
Frame = +1
Query: 262 HVDHGKTTLTAAI---TKVLSDLNLAQKKGY--ADIDNAPEEKARGITINVA 402
H+D GKTT T + T + D++ + K A +D E+ +GITI A
Sbjct: 67 HIDSGKTTFTERVLYYTGRIKDIHEVRGKDNVGAKMDFMELEREKGITIQSA 118
>SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+,
L-lysine forming] |Schizosaccharomyces pombe|chr
1|||Manual
Length = 368
Score = 25.4 bits (53), Expect = 5.6
Identities = 18/77 (23%), Positives = 31/77 (40%)
Frame = +1
Query: 136 CKCTKHNSPVRIDSIKYNTQETLCRKTNL*TYKAPLQCWQIGHVDHGKTTLTAAITKVLS 315
C T N+P+ I ++ + T PL+ + +DH T L ++ S
Sbjct: 280 CDTTNPNNPIPIYNVNTTFDHPTVEVKGV-TTPPPLE---VISIDHLPTLLPRESSEAFS 335
Query: 316 DLNLAQKKGYADIDNAP 366
+ + D+DNAP
Sbjct: 336 EALIPSLLALKDVDNAP 352
>SPAC767.01c |vps1|SPAC9G1.14c|dynamin family protein
Vps1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 678
Score = 25.4 bits (53), Expect = 5.6
Identities = 15/41 (36%), Positives = 20/41 (48%)
Frame = +1
Query: 364 PEEKARGITINVAHVEYQTEQRHYGHTDCPGHADYIKNMIT 486
PE K R IN A +YQ E G T ++ + N+IT
Sbjct: 311 PEIKVR---INAALAKYQAELHSLGDTPVGDNSSIVLNLIT 348
>SPMIT.06 |||mitochondrial DNA binding
endonuclease|Schizosaccharomyces pombe|chr
mitochondrial|||Manual
Length = 807
Score = 25.4 bits (53), Expect = 5.6
Identities = 14/51 (27%), Positives = 23/51 (45%)
Frame = +1
Query: 286 LTAAITKVLSDLNLAQKKGYADIDNAPEEKARGITINVAHVEYQTEQRHYG 438
+ A IT S + L I N+ +K + N++H + T RH+G
Sbjct: 529 ILAKITCFCSSIGLTVSPTKTKITNSYTDKILFLGTNISHSKNVTFSRHFG 579
>SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 881
Score = 25.0 bits (52), Expect = 7.3
Identities = 10/22 (45%), Positives = 11/22 (50%)
Frame = +1
Query: 400 AHVEYQTEQRHYGHTDCPGHAD 465
AH YQT+ HY T H D
Sbjct: 266 AHPIYQTQHSHYDETPTSHHPD 287
>SPBP22H7.09c |mis15||kinetochore protein Mis15 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 409
Score = 24.6 bits (51), Expect = 9.7
Identities = 12/35 (34%), Positives = 17/35 (48%)
Frame = +2
Query: 317 ISIWHRKKVMQILTMPPRRRLEVSLLMWLMSNTKP 421
I I H KK+ ++L PR L + W+ T P
Sbjct: 9 IRIKHDKKIQKLLNRFPRDFLVKLCVEWIQKQTYP 43
>SPBC4F6.11c |||asparagine synthase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 548
Score = 24.6 bits (51), Expect = 9.7
Identities = 9/15 (60%), Positives = 10/15 (66%)
Frame = +1
Query: 244 QCWQIGHVDHGKTTL 288
+ WQI H DH K TL
Sbjct: 82 EIWQINHSDHHKFTL 96
>SPBP16F5.06 |||ribosome biogenesis protein Nop6|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 478
Score = 24.6 bits (51), Expect = 9.7
Identities = 16/75 (21%), Positives = 29/75 (38%)
Frame = +2
Query: 128 SQCANVRNTTVLSGLTPLSIILKRHYAEKQIFERTKPHCNVGKSDMLITGRLHXXXXXLR 307
S+ +V N +++ TP+ I + Y + E C S+ G
Sbjct: 26 SRFGSVSNLEIINKSTPVGTIQRFAYLNFRTDEDKWQKCKSYLSNATFKGSKLRIEEARP 85
Query: 308 YYLISIWHRKKVMQI 352
YYL+ + KK+ +
Sbjct: 86 YYLVKLQQEKKIADL 100
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,266,958
Number of Sequences: 5004
Number of extensions: 45130
Number of successful extensions: 121
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 105
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 119
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 227943826
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -