Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV30839
         (791 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po...   126   4e-30
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po...   120   3e-28
SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces pomb...    30   0.33 
SPAC17H9.06c |||conserved fungal protein|Schizosaccharomyces pom...    29   0.76 
SPAC5H10.09c |||3-methyl-2-oxobutanoatehydroxymethyltransferase|...    27   3.1  
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb...    27   4.1  
SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces p...    27   4.1  
SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces p...    26   5.4  
SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi...    26   5.4  
SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom...    26   7.1  
SPAC1420.01c ||SPAC56E4.08c|DUF1752 family protein|Schizosacchar...    25   9.4  
SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosacc...    25   9.4  

>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 270

 Score =  126 bits (304), Expect = 4e-30
 Identities = 59/84 (70%), Positives = 71/84 (84%)
 Frame = +1

Query: 511 ETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQA 690
           E R    + S + Y+ A EI+ A++ PTHPIRLGLALNFSVFYYEILNSPD+AC LAKQA
Sbjct: 141 EKRQHSADQSLEGYKAASEIATAELAPTHPIRLGLALNFSVFYYEILNSPDRACYLAKQA 200

Query: 691 FDDAIAELDTLNEDSYKDSTLIMQ 762
           FD+AI+ELD+L+E+SYKDSTLIMQ
Sbjct: 201 FDEAISELDSLSEESYKDSTLIMQ 224



 Score =  117 bits (282), Expect = 2e-27
 Identities = 54/86 (62%), Positives = 70/86 (81%), Gaps = 2/86 (2%)
 Frame = +2

Query: 254 DVVGARRSSWRVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIP 427
           +V+GARR+SWR++SSIEQK E  G+  + ++ KEYR K+E+EL  IC D+L +L+KHLIP
Sbjct: 53  NVIGARRASWRIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIP 112

Query: 428 KASNPESKVFYLKMKGDYYRYLAEVA 505
            A++ ESKVFY KMKGDYYRYLAE A
Sbjct: 113 NAASAESKVFYYKMKGDYYRYLAEFA 138



 Score = 59.3 bits (137), Expect = 6e-10
 Identities = 30/50 (60%), Positives = 35/50 (70%)
 Frame = +3

Query: 105 SVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYK 254
           +  +E+ V  AKLAEQAERY+ M   MK V  T  EL+ EERNLLSVAYK
Sbjct: 3   TTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYK 52


>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 270

 Score =  120 bits (288), Expect = 3e-28
 Identities = 55/84 (65%), Positives = 69/84 (82%), Gaps = 2/84 (2%)
 Frame = +2

Query: 254 DVVGARRSSWRVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIP 427
           +++GARR+SWR+ISSIEQK E  G+ R+  + KEYR K+E EL +IC+DVL +L+KHLIP
Sbjct: 52  NIIGARRASWRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIP 111

Query: 428 KASNPESKVFYLKMKGDYYRYLAE 499
            A+  ESKVFY KMKGDYYRYLAE
Sbjct: 112 AATTGESKVFYYKMKGDYYRYLAE 135



 Score =  113 bits (273), Expect = 2e-26
 Identities = 53/84 (63%), Positives = 68/84 (80%)
 Frame = +1

Query: 511 ETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQA 690
           E      + S +AY+ A +I+ A++ PT P+RLGLALNFSVFYYEIL+SP+ AC LAKQ 
Sbjct: 140 EVCKEAADSSLEAYKAASDIAVAELPPTDPMRLGLALNFSVFYYEILDSPESACHLAKQV 199

Query: 691 FDDAIAELDTLNEDSYKDSTLIMQ 762
           FD+AI+ELD+L+E+SYKDSTLIMQ
Sbjct: 200 FDEAISELDSLSEESYKDSTLIMQ 223



 Score = 58.0 bits (134), Expect = 1e-09
 Identities = 31/51 (60%), Positives = 37/51 (72%)
 Frame = +3

Query: 102 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYK 254
           MS  +E  V  AKLAEQAERY++M   MK+V  +  +LS EERNLLSVAYK
Sbjct: 1   MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERNLLSVAYK 51


>SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 605

 Score = 30.3 bits (65), Expect = 0.33
 Identities = 16/38 (42%), Positives = 23/38 (60%)
 Frame = -1

Query: 563 KASWYAFCESSTTECLVSLWPLLQGTCSNLLSF*GKTL 450
           +A W AF E+S +E  ++L  +L    S+LLSF   TL
Sbjct: 64  EAVWAAFSEASCSERKLALQGILNNCSSSLLSFASSTL 101


>SPAC17H9.06c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 601

 Score = 29.1 bits (62), Expect = 0.76
 Identities = 26/91 (28%), Positives = 42/91 (46%), Gaps = 9/91 (9%)
 Frame = +2

Query: 284 RVISSIEQKTEGSERKQQMAKEYRVKVEKE-LREICYDVLGL--LDKHL---IPKASNPE 445
           +V+SS  +  E S  +  +    R   E E L E+  +V  L  +D+     +P+  NP 
Sbjct: 263 KVLSSSSEDDESSSPEDLLKPIIRSTEEMENLNELEQEVQDLDPIDEGFEEKVPRFRNPS 322

Query: 446 SKVFYLK---MKGDYYRYLAEVATEKPDILL 529
            K FY K   ++   Y  L  + +EK D L+
Sbjct: 323 KKAFYEKLHSLRNRSYSKLESLTSEKSDTLI 353


>SPAC5H10.09c
           |||3-methyl-2-
           oxobutanoatehydroxymethyltransferase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 267

 Score = 27.1 bits (57), Expect = 3.1
 Identities = 13/35 (37%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
 Frame = +3

Query: 471 KEITTGTLQKWPQRNQTFCC-RGFTESIPRCF*NQ 572
           K+IT  TL++W   N+ F C   +  S  R F  Q
Sbjct: 4   KQITISTLRQWKLANKKFACITAYDASFSRLFAEQ 38


>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 1207

 Score = 26.6 bits (56), Expect = 4.1
 Identities = 15/41 (36%), Positives = 24/41 (58%), Gaps = 1/41 (2%)
 Frame = +1

Query: 511  ETRHSVVEDSQKAYQDAFEISKAKMQPTHP-IRLGLALNFS 630
            E R    ED+++A + ++  + A +QP H  I L LA+N S
Sbjct: 1059 ERRRLGEEDTKRALELSYYFASADLQPMHSIIALRLAINAS 1099


>SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 355

 Score = 26.6 bits (56), Expect = 4.1
 Identities = 21/69 (30%), Positives = 37/69 (53%), Gaps = 2/69 (2%)
 Frame = +2

Query: 302 EQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKM-KGD 478
           E      E+ Q++A+ Y+V  + +LRE  YD LG +    +P A   ++  F+  +  GD
Sbjct: 43  ENPEAAREKFQKLAEAYQVLSDPKLRE-KYDKLGKVG--AVPDAGFEDAFEFFKNLFGGD 99

Query: 479 YYR-YLAEV 502
            +R Y+ E+
Sbjct: 100 SFRDYVGEL 108


>SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 517

 Score = 26.2 bits (55), Expect = 5.4
 Identities = 8/40 (20%), Positives = 20/40 (50%)
 Frame = -2

Query: 727 RSMCPVRRWHRRKPVWRVDTPCLANLISHNKRLRNLTPDP 608
           R + P+ +W R++ +W +  P    + +   ++  + P P
Sbjct: 120 RDVTPINQWKRKRSLWDIKPPGYELVTADQAKMSGVFPLP 159


>SPBC839.10 |usp107|snu71|U1 snRNP-associated protein
           Usp107|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 695

 Score = 26.2 bits (55), Expect = 5.4
 Identities = 13/42 (30%), Positives = 22/42 (52%)
 Frame = +2

Query: 305 QKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 430
           +K    +    + KEY  K +++LR+   D+  LL KH I +
Sbjct: 328 EKQRSKDEYASLYKEYTRKEQEKLRKQNDDLQNLLSKHRISR 369


>SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1067

 Score = 25.8 bits (54), Expect = 7.1
 Identities = 16/46 (34%), Positives = 21/46 (45%)
 Frame = +1

Query: 7   EKLILCLNLLVQIHFSPSDKGISELVLFHRPRCPSTRKNWCNVPNW 144
           EKL+ C       HF    +G+ +  L    RC +T    CNVP W
Sbjct: 683 EKLVACTT-----HFY---EGVRKSALSSLWRCATTYYKVCNVPQW 720


>SPAC1420.01c ||SPAC56E4.08c|DUF1752 family
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 580

 Score = 25.4 bits (53), Expect = 9.4
 Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
 Frame = +1

Query: 49  FSPSDK-GISELVLFHRPRCPSTRKNWCNVPNWPNKLSDMTT 171
           FSP +K  + +L LFH  + PS+++    V N  +  SD +T
Sbjct: 162 FSPPEKPSMKDLALFHGNKSPSSKETIPKVSN--SNSSDTST 201


>SPBC6B1.04 |mde4||monopolin-like complex subunit
           Mde4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 421

 Score = 25.4 bits (53), Expect = 9.4
 Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 2/43 (4%)
 Frame = +2

Query: 323 ERKQQMAKEYRVKVEKELREICYDV--LGLLDKHLIPKASNPE 445
           E++Q  A +YR+KVE+   +I   V  +  L+  L  + SNPE
Sbjct: 85  EQEQNEANDYRLKVERLEHKISDYVQEINSLNSQLQIQKSNPE 127


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,242,614
Number of Sequences: 5004
Number of extensions: 66041
Number of successful extensions: 232
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 220
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 230
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 385381248
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)