Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV30826
         (660 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosacchar...    89   4e-19
SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog |Schi...    49   7e-07
SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S...    31   0.11 
SPAC12G12.11c |||DUF544 family protein|Schizosaccharomyces pombe...    27   1.8  
SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1 |Schizosacch...    27   2.4  
SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyc...    26   5.5  
SPAC11H11.04 |mam2||pheromone p-factor receptor|Schizosaccharomy...    25   9.7  

>SPBC354.02c |sec61||translocon alpha subunit
           Sec61|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 479

 Score = 89.4 bits (212), Expect = 4e-19
 Identities = 47/90 (52%), Positives = 57/90 (63%), Gaps = 7/90 (7%)
 Frame = +3

Query: 3   SGISLFIATNICETIVWKAFSPATVNTGRGTEFEGAVIALFHLLATRPDKVRALREAFYR 182
           SGISLFIAT  CE I WKAFSP T +   G +FEGAVI   +++ T  +K  AL +AF+R
Sbjct: 179 SGISLFIATINCENIFWKAFSPTTYHIANGVQFEGAVINFVYVMFTWDNKAAALYQAFFR 238

Query: 183 Q-------NLPNLMNLLATVLVFAIVIYFQ 251
                    LPNL N  AT+LVF +VIY Q
Sbjct: 239 SGLTSSQIQLPNLWNFFATLLVFGVVIYLQ 268



 Score = 85.4 bits (202), Expect = 6e-18
 Identities = 38/67 (56%), Positives = 51/67 (76%)
 Frame = +2

Query: 245 LPGLRVDLPIKSARYRGQYSSYPIKLFYTSNIPIILQSALVSNLYVISQMLAVKFSGNFL 424
           L   RV++PI+S ++RG  S++P+KL YTSN PI+LQSAL SNL+  S++L  +FS NFL
Sbjct: 267 LQDFRVEIPIRSQKFRGYRSTFPVKLLYTSNTPIMLQSALTSNLFFASRLLFNRFSSNFL 326

Query: 425 VNVLGVW 445
           V  LGVW
Sbjct: 327 VRFLGVW 333



 Score = 65.7 bits (153), Expect = 6e-12
 Identities = 28/50 (56%), Positives = 36/50 (72%)
 Frame = +1

Query: 508 APPESLAHIAHDPVHALLYVLFMLGSCAFFSKTWIDVSGSSAKDVAKQLK 657
           +PP S      DP+H L+YV F + +CA FSK WI+VSG+S +DVAKQLK
Sbjct: 346 SPPASFQDALIDPIHTLVYVFFTMFACALFSKLWIEVSGASPRDVAKQLK 395


>SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 475

 Score = 48.8 bits (111), Expect = 7e-07
 Identities = 21/48 (43%), Positives = 35/48 (72%)
 Frame = +2

Query: 254 LRVDLPIKSARYRGQYSSYPIKLFYTSNIPIILQSALVSNLYVISQML 397
           +R+D+PI+S+R RG   ++P+KL YTS IP+I   +++S+L V +  L
Sbjct: 265 IRIDVPIRSSRVRGVRQNFPLKLLYTSVIPLIYFYSILSHLLVFAYAL 312



 Score = 34.3 bits (75), Expect = 0.016
 Identities = 14/50 (28%), Positives = 25/50 (50%)
 Frame = +1

Query: 511 PPESLAHIAHDPVHALLYVLFMLGSCAFFSKTWIDVSGSSAKDVAKQLKE 660
           PP  L+     PVH ++Y + ++    +FS  W++ +    +DV    KE
Sbjct: 351 PPLGLSEALLHPVHTVIYTITLICITIYFSLLWMNATAGGPRDVLLFFKE 400


>SPBC29A10.10c |||tRNA-splicing endonuclease positive effector
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1944

 Score = 31.5 bits (68), Expect = 0.11
 Identities = 21/68 (30%), Positives = 32/68 (47%), Gaps = 3/68 (4%)
 Frame = +2

Query: 218 CSCLCYSDILPGLRVDLPIKS---ARYRGQYSSYPIKLFYTSNIPIILQSALVSNLYVIS 388
           CS +C  D LP  +VD+P KS       G  +++   L  T    +  Q+ LVSN     
Sbjct: 520 CSTVCLIDKLPPKKVDIPFKSDFENALNGFVATFSEMLIQTQCWHLSAQTQLVSNPLTFR 579

Query: 389 QMLAVKFS 412
            + ++ FS
Sbjct: 580 GIFSLVFS 587


>SPAC12G12.11c |||DUF544 family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 365

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 17/53 (32%), Positives = 24/53 (45%), Gaps = 2/53 (3%)
 Frame = +3

Query: 39  ETIVWKAFSPATVNTGRGTEFEGAVIALFHLLATRPD--KVRALREAFYRQNL 191
           E +VW+ F   TV TG G       I   ++L  R +  K RA  +  Y + L
Sbjct: 273 EDVVWETFDSQTVETGNGELCAANFIPAVYVLNQRKEEKKKRAKDDEQYAKRL 325


>SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1327

 Score = 27.1 bits (57), Expect = 2.4
 Identities = 10/25 (40%), Positives = 16/25 (64%)
 Frame = -3

Query: 175 KASRRARTLSGRVANRWNNAITAPS 101
           K  RR+R LS ++ N W+  + +PS
Sbjct: 542 KLFRRSRELSEKLCNNWSERVKSPS 566


>SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 987

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 9/18 (50%), Positives = 12/18 (66%)
 Frame = +3

Query: 603 DLDRCVWLLGQRCGETTE 656
           +LD+CVW L + C E  E
Sbjct: 378 ELDKCVWFLLKNCDEFIE 395


>SPAC11H11.04 |mam2||pheromone p-factor receptor|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 348

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 14/41 (34%), Positives = 23/41 (56%)
 Frame = +3

Query: 264 ICPSSLLVIVDSILHTQSNYSTLLIYPSFCSLLLSPIFMSS 386
           I P++   I+DS +HT   +S++       SL LS ++ SS
Sbjct: 259 IVPATF-TIIDSFIHTYDGFSSMTQCLLIISLPLSSLWASS 298


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,602,188
Number of Sequences: 5004
Number of extensions: 49157
Number of successful extensions: 132
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 123
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 131
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 299817502
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)