Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30808
(806 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 159 3e-40
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 158 1e-39
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 100 2e-22
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 73 6e-14
SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe... 28 1.4
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 28 1.8
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.2
SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce... 26 7.2
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 9.6
SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces po... 25 9.6
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 159 bits (387), Expect = 3e-40
Identities = 78/176 (44%), Positives = 100/176 (56%)
Frame = +1
Query: 220 FQHFLQRDRSWQHVPRAVFVDLEPTVVDEVRTGTYRQLFHPXQLITGKEDAANNYARGHY 399
F F + VPR+++VDLEP V+D+VRTG Y+ LFHP Q++TGKEDA+NNYARGHY
Sbjct: 53 FGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHY 112
Query: 400 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTALGSLPY*WSVSPLTTARSLNWX 579
T+GKE++D VL+RIR++AD C+GLQGFL+FHSFGGGT G + + N
Sbjct: 113 TVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQ 172
Query: 580 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXWSTMKPSMNICRRNLDIERPTY 747
+ +ICRRNLDIERPTY
Sbjct: 173 FSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTY 228
Score = 125 bits (302), Expect = 6e-30
Identities = 60/99 (60%), Positives = 74/99 (74%)
Frame = +3
Query: 510 GSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVD 689
GSG +LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVD
Sbjct: 150 GSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVD 209
Query: 690 NEAIYEHLPA*SRH*APNLHQPESFIGQIVSSITASLRF 806
NEA Y+ P I Q+VSSITASLRF
Sbjct: 210 NEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRF 248
Score = 90.6 bits (215), Expect = 2e-19
Identities = 46/68 (67%), Positives = 48/68 (70%), Gaps = 5/68 (7%)
Frame = +2
Query: 77 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 241
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 242 TGAGSTYP 265
TG G P
Sbjct: 60 TGQGKFVP 67
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 158 bits (383), Expect = 1e-39
Identities = 67/100 (67%), Positives = 82/100 (82%)
Frame = +1
Query: 220 FQHFLQRDRSWQHVPRAVFVDLEPTVVDEVRTGTYRQLFHPXQLITGKEDAANNYARGHY 399
F F ++VPR+++VDLEP V+D+VRTG YR LFHP QLITGKEDA+NNYARGHY
Sbjct: 49 FSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHY 108
Query: 400 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTALG 519
T+GKE+VD V D+IR++AD C+GLQGFL+FHSFGGGT G
Sbjct: 109 TVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSG 148
Score = 123 bits (296), Expect = 3e-29
Identities = 59/99 (59%), Positives = 75/99 (75%)
Frame = +3
Query: 510 GSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVD 689
GSGF +LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVD
Sbjct: 146 GSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVD 205
Query: 690 NEAIYEHLPA*SRH*APNLHQPESFIGQIVSSITASLRF 806
NE+ Y+ P+ I Q+VSSITASLRF
Sbjct: 206 NESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRF 244
Score = 96.7 bits (230), Expect = 3e-21
Identities = 42/63 (66%), Positives = 46/63 (73%)
Frame = +2
Query: 77 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGS 256
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG G
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 257 TYP 265
P
Sbjct: 61 YVP 63
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 100 bits (240), Expect = 2e-22
Identities = 45/89 (50%), Positives = 60/89 (67%)
Frame = +1
Query: 253 QHVPRAVFVDLEPTVVDEVRTGTYRQLFHPXQLITGKEDAANNYARGHYTIGKEIVDLVL 432
++VPRAV VDLEP +D V++G + LF P +I G+ A N +A+GHYT G E+ D VL
Sbjct: 58 KYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVL 117
Query: 433 DRIRKLADQCTGLQGFLIFHSFGGGTALG 519
D +R+ A+ C LQGF + HS GGGT G
Sbjct: 118 DVVRREAEACDALQGFQLTHSLGGGTGSG 146
Score = 68.1 bits (159), Expect = 1e-12
Identities = 32/99 (32%), Positives = 55/99 (55%)
Frame = +3
Query: 510 GSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVD 689
GSG +LL+ ++ +Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +D
Sbjct: 144 GSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCID 203
Query: 690 NEAIYEHLPA*SRH*APNLHQPESFIGQIVSSITASLRF 806
NEA+ + +P+ + +++ +T S RF
Sbjct: 204 NEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRF 242
Score = 38.7 bits (86), Expect = 0.001
Identities = 20/63 (31%), Positives = 27/63 (42%)
Frame = +2
Query: 77 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGS 256
MRE + + GQ G Q+G A W EHG+ G T + N +F+E G
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 257 TYP 265
P
Sbjct: 59 YVP 61
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 72.5 bits (170), Expect = 6e-14
Identities = 37/99 (37%), Positives = 59/99 (59%), Gaps = 2/99 (2%)
Frame = +1
Query: 229 FLQRDRSWQHVPRAVFVDLEPTVVDEVRTGTYRQLFHPXQLITGKE--DAANNYARGHYT 402
F Q D + +++PRA+ +DLEP VV+ + + TY L++P ++ K A NN+A G Y+
Sbjct: 52 FYQSDDT-RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YS 109
Query: 403 IGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTALG 519
+ I + ++D I + AD L+GF + HS GGT G
Sbjct: 110 HAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSG 148
Score = 62.5 bits (145), Expect = 7e-11
Identities = 36/101 (35%), Positives = 56/101 (55%), Gaps = 2/101 (1%)
Frame = +3
Query: 510 GSGFTSLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMV 686
GSG S L+ERL+ Y KK ++++P Q VS VV+PYNS+L ++D ++
Sbjct: 146 GSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVL 205
Query: 687 DNEAIYEHLPA*SRH*A-PNLHQPESFIGQIVSSITASLRF 806
DN A+ H+ A H P HQ + ++S+ T +LR+
Sbjct: 206 DNAAL-AHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRY 245
Score = 48.8 bits (111), Expect = 9e-07
Identities = 24/55 (43%), Positives = 33/55 (60%)
Frame = +2
Query: 80 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSET 244
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQS 55
>SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 830
Score = 28.3 bits (60), Expect = 1.4
Identities = 14/46 (30%), Positives = 26/46 (56%)
Frame = -3
Query: 564 SCRSQRRDAPSIRK*TQSRTSTEGVEDQESLQTGTLVSELADSVQN 427
S + SIR ++SR+S+ G + Q S+Q+GT ++D ++
Sbjct: 98 SSQKSSNSRKSIRSQSRSRSSSVGSDSQASIQSGTNSEVVSDDEED 143
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 27.9 bits (59), Expect = 1.8
Identities = 16/37 (43%), Positives = 20/37 (54%), Gaps = 2/37 (5%)
Frame = -3
Query: 312 ADLINNSRFKIDEDS--TGYVLPAPVSLKKVLKESSP 208
AD+IN SR+ ID S T + P+SL KE P
Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQP 491
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.2
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 175 WPDAHRQDHRGWRRFFQHFLQR 240
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1067
Score = 25.8 bits (54), Expect = 7.2
Identities = 12/37 (32%), Positives = 23/37 (62%)
Frame = -3
Query: 279 DEDSTGYVLPAPVSLKKVLKESSPPPMVLSVGIWPSG 169
++D T Y+ P P+ + L +++PP +V+ +G P G
Sbjct: 834 EKDLTKYLGP-PIYTSQRLYDTTPPGVVMGLGWTPMG 869
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 9.6
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +3
Query: 66 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 188
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
>SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 399
Score = 25.4 bits (53), Expect = 9.6
Identities = 13/53 (24%), Positives = 24/53 (45%), Gaps = 2/53 (3%)
Frame = -3
Query: 330 LSVCASADLINNSRF--KIDEDSTGYVLPAPVSLKKVLKESSPPPMVLSVGIW 178
+ + S +++N+ + KID GY S K + E + P + G+W
Sbjct: 210 IELYGSTYIVSNTNYITKIDYSGRGYFRGTKNSFKATIFEKNEDPDYIVEGVW 262
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,346,100
Number of Sequences: 5004
Number of extensions: 70575
Number of successful extensions: 217
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 194
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 209
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 392429240
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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