Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV30786
         (653 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef...   134   1e-32
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef...   134   1e-32
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef...   134   1e-32
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa...    44   2e-05
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote...    32   0.083
SPBC691.01 |||palmitoyltransferase |Schizosaccharomyces pombe|ch...    31   0.15 
SPAP8A3.06 |||U2AF small subunit, U2AF-23|Schizosaccharomyces po...    26   4.1  
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos...    25   7.2  
SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2...    25   9.5  
SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc...    25   9.5  

>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
           Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 460

 Score =  134 bits (323), Expect = 1e-32
 Identities = 59/79 (74%), Positives = 67/79 (84%)
 Frame = +1

Query: 1   GYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRR 180
           G V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR
Sbjct: 321 GNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRR 380

Query: 181 TGKSTEVNPKSIKSGDAAI 237
           +GK  E +PK +KSGDA I
Sbjct: 381 SGKKIEESPKFVKSGDACI 399



 Score = 63.7 bits (148), Expect = 2e-11
 Identities = 28/34 (82%), Positives = 32/34 (94%)
 Frame = +3

Query: 255 KPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 356
           KP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 406 KPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439


>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
           Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 460

 Score =  134 bits (323), Expect = 1e-32
 Identities = 59/79 (74%), Positives = 67/79 (84%)
 Frame = +1

Query: 1   GYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRR 180
           G V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR
Sbjct: 321 GNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRR 380

Query: 181 TGKSTEVNPKSIKSGDAAI 237
           +GK  E +PK +KSGDA I
Sbjct: 381 SGKKIEESPKFVKSGDACI 399



 Score = 63.7 bits (148), Expect = 2e-11
 Identities = 28/34 (82%), Positives = 32/34 (94%)
 Frame = +3

Query: 255 KPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 356
           KP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 406 KPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439


>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
           Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 460

 Score =  134 bits (323), Expect = 1e-32
 Identities = 59/79 (74%), Positives = 67/79 (84%)
 Frame = +1

Query: 1   GYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRR 180
           G V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR
Sbjct: 321 GNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRR 380

Query: 181 TGKSTEVNPKSIKSGDAAI 237
           +GK  E +PK +KSGDA I
Sbjct: 381 SGKKIEESPKFVKSGDACI 399



 Score = 63.7 bits (148), Expect = 2e-11
 Identities = 28/34 (82%), Positives = 32/34 (94%)
 Frame = +3

Query: 255 KPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 356
           KP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 406 KPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439


>SPCC584.04 |sup35|erf3|translation release factor eRF3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 662

 Score = 44.0 bits (99), Expect = 2e-05
 Identities = 23/64 (35%), Positives = 34/64 (53%)
 Frame = +1

Query: 1   GYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRR 180
           GYV   +KN P      F AQ+ +L  P  ++ GY+ V+  HTA     FA++  K+D+ 
Sbjct: 544 GYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLDKT 602

Query: 181 TGKS 192
             KS
Sbjct: 603 NRKS 606



 Score = 39.9 bits (89), Expect = 3e-04
 Identities = 18/41 (43%), Positives = 29/41 (70%), Gaps = 1/41 (2%)
 Frame = +3

Query: 240 QLGTFKPLCVESFQEFPPLGRFAVRDMRQTVAVG-VIKAVN 359
           +L T  P+C+E F+++  +GRF +RD   TVAVG V+K ++
Sbjct: 622 ELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662


>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 592

 Score = 31.9 bits (69), Expect = 0.083
 Identities = 15/31 (48%), Positives = 20/31 (64%)
 Frame = +3

Query: 258 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 350
           PLC+   +E P LGRF +R    TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589


>SPBC691.01 |||palmitoyltransferase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 312

 Score = 31.1 bits (67), Expect = 0.15
 Identities = 14/36 (38%), Positives = 18/36 (50%)
 Frame = +1

Query: 448 FSYNCDTSFTERCFEGQKGYNSFLFYIFYKACNVTL 555
           F + C     + CF  QK +  FLFY F  AC V +
Sbjct: 139 FDHYCSFVGKDVCFSNQKFFYQFLFYGFSAACMVLI 174


>SPAP8A3.06 |||U2AF small subunit, U2AF-23|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 216

 Score = 26.2 bits (55), Expect = 4.1
 Identities = 12/34 (35%), Positives = 14/34 (41%)
 Frame = +2

Query: 239 STWYLQASMCRVLPGIPTPRSFCCP*HEADSCCR 340
           S WY Q  +   L  +   R  CC  HE   C R
Sbjct: 126 SRWYSQRPVYAELSPVTDFREACCRQHETSECQR 159


>SPAC631.01c |acp2||F-actin capping protein beta subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 268

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 11/25 (44%), Positives = 18/25 (72%)
 Frame = -1

Query: 236 MAASPDLMDFGLTSVDLPVRRSTFS 162
           ++ +PDL D  L+SVD P++ +T S
Sbjct: 27  LSVAPDLADVLLSSVDQPLKVNTCS 51


>SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 244

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 10/37 (27%), Positives = 19/37 (51%)
 Frame = -2

Query: 415 PLVAFSAALVTLPPPASLKLTALMTPTATVCLMSRTA 305
           P  + ++   T+PP  S+  T  + PT ++   + TA
Sbjct: 73  PSTSHNSTTTTVPPTTSMNTTTTVPPTTSLNTTTTTA 109


>SPBC18H10.04c |sce3|tif48|translation initiation factor
           eIF4B|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 388

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 13/41 (31%), Positives = 20/41 (48%)
 Frame = -2

Query: 388 VTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDST 266
           + L P +S  +    TP+AT    S+  + P GG    D+T
Sbjct: 244 LNLKPRSSSNVNTEATPSATTTTSSKPKRDPFGGAKPVDNT 284


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,547,994
Number of Sequences: 5004
Number of extensions: 49733
Number of successful extensions: 146
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 137
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 146
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)