Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30702
(661 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 122 5e-29
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 118 6e-28
SPBC11C11.09c |rpl502|rpl5-2, rpl5b|60S ribosomal protein L5|Sch... 29 0.78
SPAC3H5.12c |rpl501|rpl5-1, rpl5|60S ribosomal protein L5|Schizo... 29 0.78
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 3.2
SPBC11C11.08 |srp1||SR family protein Srp1|Schizosaccharomyces p... 26 4.2
SPCC126.01c ||SPCC576.18c|conserved fungal protein|Schizosacchar... 26 5.5
SPCC830.03 |||AAA family ATPase Grc3 |Schizosaccharomyces pombe|... 25 7.3
SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomy... 25 7.3
SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr... 25 7.3
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 122 bits (294), Expect = 5e-29
Identities = 62/110 (56%), Positives = 77/110 (70%)
Frame = +2
Query: 173 RRPPRLLADHLLHRTEGRDKRRRGQIELIRAYRSQVEKELRDICSDILGVLDKYLIPSSQ 352
RR + + + E R R Q LI+ YR ++E EL DIC D+L VL+K+LIP++
Sbjct: 57 RRASWRIISSIEQKEESRGNTR--QAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAAT 114
Query: 353 TEESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTEL 502
T ESKVFYYKMKGDY+RYLAEF G KEAA++SL AYKAASDIA+ EL
Sbjct: 115 TGESKVFYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAEL 164
Score = 111 bits (268), Expect = 7e-26
Identities = 56/76 (73%), Positives = 67/76 (88%)
Frame = +3
Query: 9 SEREDNVYKAKLADQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARRA 188
+ RE++VY AKLA+QAERY+EMVE MK VA N ++L+VEERNLLSVAYKN+IGARRA
Sbjct: 3 NSRENSVYLAKLAEQAERYEEMVENMKKVACSN---DKLSVEERNLLSVAYKNIIGARRA 59
Query: 189 SWRIISSIEQKEETKG 236
SWRIISSIEQKEE++G
Sbjct: 60 SWRIISSIEQKEESRG 75
Score = 83.4 bits (197), Expect = 3e-17
Identities = 38/52 (73%), Positives = 44/52 (84%)
Frame = +1
Query: 505 PPHPIRLGLALNFSVFYYEILNSPDRACRLGKAAFYDAIAELDTLSEESYKD 660
P P+RLGLALNFSVFYYEIL+SP+ AC L K F +AI+ELD+LSEESYKD
Sbjct: 166 PTDPMRLGLALNFSVFYYEILDSPESACHLAKQVFDEAISELDSLSEESYKD 217
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 118 bits (285), Expect = 6e-28
Identities = 55/91 (60%), Positives = 70/91 (76%)
Frame = +2
Query: 230 KRRRGQIELIRAYRSQVEKELRDICSDILGVLDKYLIPSSQTEESKVFYYKMKGDYHRYL 409
K Q+ELI+ YR ++E+EL IC DIL VL+K+LIP++ + ESKVFYYKMKGDY+RYL
Sbjct: 75 KGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVFYYKMKGDYYRYL 134
Query: 410 AEFATGNDRKEAAENSLVAYKAASDIAMTEL 502
AEFA G R+ +A+ SL YKAAS+IA EL
Sbjct: 135 AEFAVGEKRQHSADQSLEGYKAASEIATAEL 165
Score = 117 bits (281), Expect = 2e-27
Identities = 60/76 (78%), Positives = 67/76 (88%)
Frame = +3
Query: 9 SEREDNVYKAKLADQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARRA 188
+ RED VY AKLA+QAERY+ MVE MK+VAS +D ELTVEERNLLSVAYKNVIGARRA
Sbjct: 4 TSREDAVYLAKLAEQAERYEGMVENMKSVAS---TDQELTVEERNLLSVAYKNVIGARRA 60
Query: 189 SWRIISSIEQKEETKG 236
SWRI+SSIEQKEE+KG
Sbjct: 61 SWRIVSSIEQKEESKG 76
Score = 96.7 bits (230), Expect = 3e-21
Identities = 45/53 (84%), Positives = 48/53 (90%)
Frame = +1
Query: 502 APPHPIRLGLALNFSVFYYEILNSPDRACRLGKAAFYDAIAELDTLSEESYKD 660
AP HPIRLGLALNFSVFYYEILNSPDRAC L K AF +AI+ELD+LSEESYKD
Sbjct: 166 APTHPIRLGLALNFSVFYYEILNSPDRACYLAKQAFDEAISELDSLSEESYKD 218
>SPBC11C11.09c |rpl502|rpl5-2, rpl5b|60S ribosomal protein
L5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 294
Score = 28.7 bits (61), Expect = 0.78
Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 3/64 (4%)
Frame = +1
Query: 445 CRELAGRVQGS--LRHRHDRTAPPHPIRLGLALNFSVFYYEILNSPDRA-CRLGKAAFYD 615
C+ ++ RV G L H H P + I+ GLA N++ Y L RA ++G A Y+
Sbjct: 62 CQIVSSRVNGDYVLAHAHSSELPRYGIKWGLA-NWTAAYATGLLVARRALAKVGLADKYE 120
Query: 616 AIAE 627
+ E
Sbjct: 121 GVTE 124
>SPAC3H5.12c |rpl501|rpl5-1, rpl5|60S ribosomal protein
L5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 294
Score = 28.7 bits (61), Expect = 0.78
Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 3/64 (4%)
Frame = +1
Query: 445 CRELAGRVQGS--LRHRHDRTAPPHPIRLGLALNFSVFYYEILNSPDRA-CRLGKAAFYD 615
C+ ++ RV G L H H P + I+ GLA N++ Y L RA ++G A Y+
Sbjct: 62 CQIVSSRVNGDYVLAHAHSSELPRYGIKWGLA-NWTAAYATGLLVARRALAKVGLADKYE 120
Query: 616 AIAE 627
+ E
Sbjct: 121 GVTE 124
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 3.2
Identities = 11/30 (36%), Positives = 21/30 (70%)
Frame = +2
Query: 197 DHLLHRTEGRDKRRRGQIELIRAYRSQVEK 286
D L +G + +RRG+++LI+ ++SQ+ K
Sbjct: 960 DGSLAELKGFEVKRRGELKLIKDFQSQIFK 989
>SPBC11C11.08 |srp1||SR family protein Srp1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 275
Score = 26.2 bits (55), Expect = 4.2
Identities = 12/29 (41%), Positives = 14/29 (48%)
Frame = +1
Query: 436 QGGCRELAGRVQGSLRHRHDRTAPPHPIR 522
+GG RE GRV G R+ PH R
Sbjct: 96 RGGRRERGGRVHGDSGRLRSRSPSPHEAR 124
>SPCC126.01c ||SPCC576.18c|conserved fungal
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 369
Score = 25.8 bits (54), Expect = 5.5
Identities = 14/53 (26%), Positives = 26/53 (49%)
Frame = +2
Query: 215 TEGRDKRRRGQIELIRAYRSQVEKELRDICSDILGVLDKYLIPSSQTEESKVF 373
T DK R + L + + E D+C+D+L + D + + + EE +V+
Sbjct: 240 TASADKTVR-EWSLYQGIHEETRMEHPDVCTDVLTLADGNIATACRDEEIRVW 291
>SPCC830.03 |||AAA family ATPase Grc3 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 736
Score = 25.4 bits (53), Expect = 7.3
Identities = 15/43 (34%), Positives = 20/43 (46%), Gaps = 2/43 (4%)
Frame = -2
Query: 126 SAHCRTRCVMLHSSSPPPFRHSAQPGQLTLLYT--HYLPFPTL 4
S+H + + H P F S QLTLL + YLP P +
Sbjct: 486 SSHREKKSIYQHKEYMPDFLSSRDEFQLTLLESTWQYLPDPNV 528
>SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 542
Score = 25.4 bits (53), Expect = 7.3
Identities = 17/59 (28%), Positives = 33/59 (55%), Gaps = 1/59 (1%)
Frame = +3
Query: 33 KAKLADQAERYDEMVEAMKNVASRNVSDNELTVEERN-LLSVAYKNVIGARRASWRIIS 206
+ K D+ E + + +++K+V S + +D E++ +E N LL + YK V ++W S
Sbjct: 12 QGKFNDKEEGFSNL-KSLKHV-SHSETDFEVSNDEDNQLLELGYKPVFKREFSTWATFS 68
>SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 661
Score = 25.4 bits (53), Expect = 7.3
Identities = 17/57 (29%), Positives = 28/57 (49%), Gaps = 4/57 (7%)
Frame = +3
Query: 27 VYKAKLADQAE----RYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARR 185
+YK KL E R DE+V MKN+ +N + +EL + ++N + + R
Sbjct: 202 IYKEKLRFSLESRKSRVDEIVNEMKNMCGKNPT-SELPTNYSDKTLTLWENELSSMR 257
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,097,475
Number of Sequences: 5004
Number of extensions: 37208
Number of successful extensions: 164
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 299817502
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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