Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30639
(488 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g46430.2 68418.m05716 60S ribosomal protein L32 (RPL32B) 72 2e-13
At5g46430.1 68418.m05715 60S ribosomal protein L32 (RPL32B) 72 2e-13
At4g18100.1 68417.m02692 60S ribosomal protein L32 (RPL32A) ribo... 72 2e-13
At1g26570.1 68414.m03237 UDP-glucose 6-dehydrogenase, putative s... 29 1.3
At1g22880.1 68414.m02856 glycosyl hydrolase family 9 protein sim... 29 1.7
At4g07670.1 68417.m01203 protease-associated (PA) domain-contain... 28 2.9
At5g15430.1 68418.m01806 calmodulin-binding protein-related has ... 27 5.1
At4g18060.1 68417.m02687 SH3 domain-containing protein 3 (SH3P3)... 27 5.1
At2g37370.1 68415.m04583 hypothetical protein 27 5.1
At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam d... 27 6.8
At5g51950.1 68418.m06447 glucose-methanol-choline (GMC) oxidored... 27 6.8
At1g53350.1 68414.m06048 disease resistance protein (CC-NBS-LRR ... 27 6.8
At5g39320.1 68418.m04761 UDP-glucose 6-dehydrogenase, putative v... 27 9.0
>At5g46430.2 68418.m05716 60S ribosomal protein L32 (RPL32B)
Length = 133
Score = 72.1 bits (169), Expect = 2e-13
Identities = 30/43 (69%), Positives = 35/43 (81%)
Frame = +3
Query: 246 YAPNGFRKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLI 374
Y PNGF+K +VHN ELE+LMM NR YCAEIAH +S+KKRK I
Sbjct: 67 YLPNGFKKFIVHNTSELELLMMHNRTYCAEIAHNISTKKRKAI 109
Score = 56.4 bits (130), Expect = 1e-08
Identities = 37/91 (40%), Positives = 52/91 (57%), Gaps = 2/91 (2%)
Frame = +1
Query: 181 QRFKGQYLMPNIGYGSNKKTRHMLPMDSVRS*FTMLKSWKS**CKTGST--AQRSLTVSL 354
++FKG LMPN+GYGS+KKTRH LP + + S T A+ + +S
Sbjct: 45 RKFKGVTLMPNVGYGSDKKTRHYLP-NGFKKFIVHNTSELELLMMHNRTYCAEIAHNIST 103
Query: 355 RRSGS*FVERAQQLSIRVTNAAARLRSQENE 447
++ + VERA QL + V+N RLRSQE+E
Sbjct: 104 KKRKA-IVERASQLDVVVSNKLGRLRSQEDE 133
Score = 50.8 bits (116), Expect = 5e-07
Identities = 21/35 (60%), Positives = 29/35 (82%)
Frame = +2
Query: 77 IVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVR 181
+VKKR+ +FIR QSDR +K +WR+P+GID+RVR
Sbjct: 10 VVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVR 44
>At5g46430.1 68418.m05715 60S ribosomal protein L32 (RPL32B)
Length = 133
Score = 72.1 bits (169), Expect = 2e-13
Identities = 30/43 (69%), Positives = 35/43 (81%)
Frame = +3
Query: 246 YAPNGFRKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLI 374
Y PNGF+K +VHN ELE+LMM NR YCAEIAH +S+KKRK I
Sbjct: 67 YLPNGFKKFIVHNTSELELLMMHNRTYCAEIAHNISTKKRKAI 109
Score = 56.4 bits (130), Expect = 1e-08
Identities = 37/91 (40%), Positives = 52/91 (57%), Gaps = 2/91 (2%)
Frame = +1
Query: 181 QRFKGQYLMPNIGYGSNKKTRHMLPMDSVRS*FTMLKSWKS**CKTGST--AQRSLTVSL 354
++FKG LMPN+GYGS+KKTRH LP + + S T A+ + +S
Sbjct: 45 RKFKGVTLMPNVGYGSDKKTRHYLP-NGFKKFIVHNTSELELLMMHNRTYCAEIAHNIST 103
Query: 355 RRSGS*FVERAQQLSIRVTNAAARLRSQENE 447
++ + VERA QL + V+N RLRSQE+E
Sbjct: 104 KKRKA-IVERASQLDVVVSNKLGRLRSQEDE 133
Score = 50.8 bits (116), Expect = 5e-07
Identities = 21/35 (60%), Positives = 29/35 (82%)
Frame = +2
Query: 77 IVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVR 181
+VKKR+ +FIR QSDR +K +WR+P+GID+RVR
Sbjct: 10 VVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVR 44
>At4g18100.1 68417.m02692 60S ribosomal protein L32 (RPL32A)
ribosomal protein L32, human, PIR1:R5HU32
Length = 133
Score = 72.1 bits (169), Expect = 2e-13
Identities = 31/43 (72%), Positives = 35/43 (81%)
Frame = +3
Query: 246 YAPNGFRKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLI 374
Y PNGF+K +VHN ELE+LMM NR YCAEIAH VS+KKRK I
Sbjct: 67 YLPNGFKKFVVHNTSELELLMMHNRTYCAEIAHNVSTKKRKAI 109
Score = 59.7 bits (138), Expect = 1e-09
Identities = 40/91 (43%), Positives = 53/91 (58%), Gaps = 2/91 (2%)
Frame = +1
Query: 181 QRFKGQYLMPNIGYGSNKKTRHMLPMDSVRS*FTMLKSWKS**CKTGST--AQRSLTVSL 354
++FKG LMPN+GYGS+KKTRH LP + + S T A+ + VS
Sbjct: 45 RKFKGVTLMPNVGYGSDKKTRHYLP-NGFKKFVVHNTSELELLMMHNRTYCAEIAHNVST 103
Query: 355 RRSGS*FVERAQQLSIRVTNAAARLRSQENE 447
++ + VERA QL + VTN ARLRSQE+E
Sbjct: 104 KKRKA-IVERASQLDVVVTNRLARLRSQEDE 133
Score = 50.8 bits (116), Expect = 5e-07
Identities = 21/35 (60%), Positives = 29/35 (82%)
Frame = +2
Query: 77 IVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVR 181
+VKKR+ +FIR QSDR +K +WR+P+GID+RVR
Sbjct: 10 VVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVR 44
>At1g26570.1 68414.m03237 UDP-glucose 6-dehydrogenase, putative
strong similarity to SP|Q96558 UDP-glucose
6-dehydrogenase (EC 1.1.1.22) (UDP-Glc dehydrogenase)
(UDP-GlcDH) (UDPGDH) {Glycine max}; contains Pfam
profiles PF03721: UDP-glucose/GDP-mannose dehydrogenase
family NAD binding domain, PF00984:
UDP-glucose/GDP-mannose dehydrogenase family central
domain, PF03720: UDP-glucose/GDP-mannose dehydrogenase
family UDP binding domain
Length = 481
Score = 29.5 bits (63), Expect = 1.3
Identities = 24/98 (24%), Positives = 42/98 (42%), Gaps = 3/98 (3%)
Frame = +3
Query: 111 INRIAMTNLRGIGVNLEVLTTESAAIQGSILDAQHRLRFQQED-PSYAPNGFRKVLVHNV 287
+N T G G ++ ESAA + + ++ ++ P K+L HN
Sbjct: 86 VNTPTKTQGLGAGKAADLTYWESAARMIADVSKSSKIVVEKSTVPVRTAEAIEKILTHNS 145
Query: 288 KELEILMMQNRKYCAE--IAHGVSSKKRKLIRGKSPAA 395
K +E ++ N ++ AE + + R LI G+ AA
Sbjct: 146 KGIEFQILSNPEFLAEGTAIKDLYNPDRVLIGGRDTAA 183
>At1g22880.1 68414.m02856 glycosyl hydrolase family 9 protein
similar to GB:AAB65156 and GB:AAA96135
Length = 484
Score = 29.1 bits (62), Expect = 1.7
Identities = 13/42 (30%), Positives = 20/42 (47%)
Frame = -1
Query: 443 FSWDRRRAAAFVTLMLSCWALSTNQLPLLRRDTVSDLCAVLP 318
FSWD + A A+V L N L ++ + +C +LP
Sbjct: 268 FSWDNKYAGAYVLLSRRAVLNKDNNFELYKQAAENFMCKILP 309
>At4g07670.1 68417.m01203 protease-associated (PA) domain-containing
protein similar to PF02225: PA domain; similar to
N-acetylated-alpha-linked acidic dipeptidase II
(NAALADase II) (SP:Q9Y3Q) {Homo sapiens};similar to
Glutamate carboxypeptidase II (Membrane glutamate
carboxypeptidase) (mGCP) (N-acetylated-alpha-linked
acidic dipeptidase I) (NAALADase I)
(Pteroylpoly-gamma-glutamate
carboxypeptidase)(Folylpoly-gamma-glutamate
carboxypeptidase) (FGCP) (Folate hydrolase 1)
(Prostate-specific membrane antigen homolog) (SP|O77564)
{Sus scrofa}
Length = 280
Score = 28.3 bits (60), Expect = 2.9
Identities = 10/32 (31%), Positives = 21/32 (65%)
Frame = +2
Query: 65 TGPTIVKKRTKRFIRHQSDRYDKLKRNWRKPR 160
+G ++ + +K +++H + R DKL++ KPR
Sbjct: 199 SGTAVLMEASKSYLQHIAQRLDKLQKRGWKPR 230
>At5g15430.1 68418.m01806 calmodulin-binding protein-related has
weak similarity to calmodulin-binding proteins
Length = 478
Score = 27.5 bits (58), Expect = 5.1
Identities = 17/53 (32%), Positives = 26/53 (49%)
Frame = +3
Query: 246 YAPNGFRKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLIRGKSPAAQHQ 404
Y G K +V + + L ++ AEIA S+ KRK + G S AA+ +
Sbjct: 159 YVSRGRAKEIVEQKRRVTALKLKAVAQTAEIALRRSTVKRKKMNGGSKAAEQK 211
>At4g18060.1 68417.m02687 SH3 domain-containing protein 3 (SH3P3)
nearly identical to SH3 domain-containing protein 3
[Arabidopsis thaliana] GI:16974680; contains Pfam
profile PF00018: SH3 domain
Length = 351
Score = 27.5 bits (58), Expect = 5.1
Identities = 12/39 (30%), Positives = 22/39 (56%)
Frame = +3
Query: 117 RIAMTNLRGIGVNLEVLTTESAAIQGSILDAQHRLRFQQ 233
++A ++ + N+ VL E+ A ++ QHRL FQ+
Sbjct: 194 QLAEAKMQELKANMAVLGKEATAALAAVESQQHRLTFQR 232
>At2g37370.1 68415.m04583 hypothetical protein
Length = 697
Score = 27.5 bits (58), Expect = 5.1
Identities = 18/69 (26%), Positives = 32/69 (46%), Gaps = 1/69 (1%)
Frame = +3
Query: 99 DLSGINRIAMTNLRGIGVNLEVLTTESAAIQGSILDAQHRLRFQQEDPSYAPNGFRKVLV 278
++ + + L+ + + E L E + SI A+ RLR QE+ N ++L+
Sbjct: 344 EVGQLEKDVAAELKKLEILKEDLEAELKRVNTSITSARARLRNAQEEREQFDNASNEILM 403
Query: 279 H-NVKELEI 302
H KE E+
Sbjct: 404 HLKSKEEEL 412
>At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam domain
PF04931: DNA polymerase V
Length = 1306
Score = 27.1 bits (57), Expect = 6.8
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = +3
Query: 267 KVLVHNVKELEILMMQNRKYCAEIAHGVSSKK 362
K LV N+ E ++ Q RK+C I VSS K
Sbjct: 1242 KELVGNMPEAKVRRAQVRKFCGRIFQMVSSLK 1273
>At5g51950.1 68418.m06447 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 586
Score = 27.1 bits (57), Expect = 6.8
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = -3
Query: 255 WEHMTGLLVGTVTDVGHQVLTLESLRTL 172
W + G VG V D ++VL ++SLR +
Sbjct: 517 WHYHGGCQVGRVVDKNYRVLGIDSLRVI 544
>At1g53350.1 68414.m06048 disease resistance protein (CC-NBS-LRR
class), putative domain signature CC-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 906
Score = 27.1 bits (57), Expect = 6.8
Identities = 17/57 (29%), Positives = 31/57 (54%), Gaps = 1/57 (1%)
Frame = +3
Query: 258 GFRKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLIRG-KSPAAQHQSDECGRS 425
G K + V+ L ++ RK+ ++I G++ + ++I G +S QH +D GRS
Sbjct: 86 GKEKGIKKQVRTLACFLVDRRKFASDI-EGITKRISEVIVGMQSLGIQHIADGGGRS 141
>At5g39320.1 68418.m04761 UDP-glucose 6-dehydrogenase, putative very
strong similarity to SP|Q96558 UDP-glucose
6-dehydrogenase (EC 1.1.1.22) (UDP-Glc dehydrogenase)
(UDP-GlcDH) (UDPGDH) {Glycine max}; contains Pfam
profiles PF03721: UDP-glucose/GDP-mannose dehydrogenase
family NAD binding domain, PF00984:
UDP-glucose/GDP-mannose dehydrogenase family central
domain, PF03720: UDP-glucose/GDP-mannose dehydrogenase
family UDP binding domain
Length = 480
Score = 26.6 bits (56), Expect = 9.0
Identities = 17/76 (22%), Positives = 34/76 (44%), Gaps = 1/76 (1%)
Frame = +3
Query: 111 INRIAMTNLRGIGVNLEVLTTESAAIQGSILDAQHRLRFQQED-PSYAPNGFRKVLVHNV 287
+N T G G ++ ESAA + + ++ ++ P K+L+HN
Sbjct: 86 VNTPTKTTGLGAGKAADLTYWESAARMIADVSVSDKIVVEKSTVPVKTAEAIEKILMHNS 145
Query: 288 KELEILMMQNRKYCAE 335
K ++ ++ N ++ AE
Sbjct: 146 KGIKFQILSNPEFLAE 161
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,042,749
Number of Sequences: 28952
Number of extensions: 195376
Number of successful extensions: 650
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 629
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 650
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 848837888
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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