Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV30623
         (643 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAP8A3.09c |paa1||protein phosphatase regulatory subunit Paa1|S...    95   8e-21
SPAC1952.14c |mrpl25||mitochondrial ribosomal protein subunit L2...    29   0.75 
SPBP8B7.21 |ubp3||ubiquitin C-terminal hydrolase Ubp3|Schizosacc...    26   5.3  
SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos...    25   7.0  
SPBC1604.12 |||sequence orphan|Schizosaccharomyces pombe|chr 2||...    25   9.3  

>SPAP8A3.09c |paa1||protein phosphatase regulatory subunit
           Paa1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 590

 Score = 95.1 bits (226), Expect = 8e-21
 Identities = 50/93 (53%), Positives = 59/93 (63%), Gaps = 1/93 (1%)
 Frame = +1

Query: 1   AAVEETVVRDKAVASLRAVAEHHSPQALEEHFVPLVQRLAGGDWFTSRTSACGLFSVCYP 180
           AA EETVVRDKAV SL  V    S + LE++FVPLVQRL+  +WFTSR S+ GL+   Y 
Sbjct: 97  AATEETVVRDKAVDSLNKVCICLSQEQLEQYFVPLVQRLSTAEWFTSRASSAGLYCAAYS 156

Query: 181 RVSAP-VKAELRQHFRSLCQDDTPMVAALLPTN 276
           +   P VK  LRQ F  LC D+ PMV     TN
Sbjct: 157 QSENPAVKVSLRQSFSHLCHDEAPMVRRPAATN 189



 Score = 50.4 bits (115), Expect = 2e-07
 Identities = 28/86 (32%), Positives = 43/86 (50%), Gaps = 1/86 (1%)
 Frame = +3

Query: 255 RRAAAYKLGEFAKVVEIEYVKSDLIPIFVILAEDDQDSVR-XXXXXXXXXXXXXXTPEDM 431
           RR AA    +F  +V  +    + IP+F  L+ DDQDSVR               +  ++
Sbjct: 183 RRPAATNCAKFVFLVTKQEAIDEFIPLFNSLSNDDQDSVRLLSFDIMVSLAEVLKSDSEI 242

Query: 432 EQHVMPTVRALSGDASWRVRYMVADN 509
             +++  +R+   D+SWR RYMVA N
Sbjct: 243 RHYLLQPLRSFVSDSSWRTRYMVAAN 268



 Score = 32.3 bits (70), Expect = 0.061
 Identities = 21/85 (24%), Positives = 35/85 (41%)
 Frame = +3

Query: 255 RRAAAYKLGEFAKVVEIEYVKSDLIPIFVILAEDDQDSVRXXXXXXXXXXXXXXTPEDME 434
           R   A    + AKVV    +K +LI  FV+L +D +  VR                  + 
Sbjct: 262 RYMVAANFVKLAKVVGPSLIKDELIKPFVLLMKDTEQEVRRAIATQIPGFCELLDKRIVL 321

Query: 435 QHVMPTVRALSGDASWRVRYMVADN 509
           + ++P ++ L  D +  VR  +  N
Sbjct: 322 EEIIPVIQELINDPAQHVRAALGMN 346



 Score = 29.9 bits (64), Expect = 0.33
 Identities = 17/34 (50%), Positives = 19/34 (55%)
 Frame = +3

Query: 273 KLGEFAKVVEIEYVKSDLIPIFVILAEDDQDSVR 374
           KL    KVV IE +   L+P  V LAED Q  VR
Sbjct: 385 KLEVVNKVVGIELLSQSLLPAIVTLAEDKQWRVR 418


>SPAC1952.14c |mrpl25||mitochondrial ribosomal protein subunit
           L25|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 148

 Score = 28.7 bits (61), Expect = 0.75
 Identities = 16/58 (27%), Positives = 32/58 (55%), Gaps = 3/58 (5%)
 Frame = -2

Query: 369 RNLDHPLPKSQIWE*DRS*HIQSPQLSQTLQVCRQQRG---DHRSIILTEGAEMLTKF 205
           +NLD+ LP+   W+ D + HI    L+   ++  ++R    ++R + L+E  +  +KF
Sbjct: 72  QNLDNLLPQQMSWQKDSTRHILKGLLNPKGKISERKRDEILENRRLKLSESLKKTSKF 129


>SPBP8B7.21 |ubp3||ubiquitin C-terminal hydrolase
           Ubp3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 512

 Score = 25.8 bits (54), Expect = 5.3
 Identities = 12/30 (40%), Positives = 14/30 (46%)
 Frame = +1

Query: 46  LRAVAEHHSPQALEEHFVPLVQRLAGGDWF 135
           L AV  HH   A   H+   VQ+L    WF
Sbjct: 438 LNAVVYHHGTSASGGHYTVDVQQLDKSGWF 467


>SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 855

 Score = 25.4 bits (53), Expect = 7.0
 Identities = 13/33 (39%), Positives = 15/33 (45%)
 Frame = +3

Query: 54  RCRAPQPASTRGTLCATSTAPCWRRLVHFKNIC 152
           R  A  P  T  T C T T P WRR    + +C
Sbjct: 625 RANATNPTPTC-TNCQTRTTPLWRRSPDGQPLC 656


>SPBC1604.12 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 860

 Score = 25.0 bits (52), Expect = 9.3
 Identities = 10/27 (37%), Positives = 14/27 (51%)
 Frame = -2

Query: 414 IEPQQPRRLRQPADERNLDHPLPKSQI 334
           I+P  PR    P   R+  HP+P  Q+
Sbjct: 599 IDPSSPRDDSTPLSWRSHSHPIPPVQV 625


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,457,991
Number of Sequences: 5004
Number of extensions: 49250
Number of successful extensions: 138
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 287744314
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)