BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV30623 (643 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAP8A3.09c |paa1||protein phosphatase regulatory subunit Paa1|S... 95 8e-21 SPAC1952.14c |mrpl25||mitochondrial ribosomal protein subunit L2... 29 0.75 SPBP8B7.21 |ubp3||ubiquitin C-terminal hydrolase Ubp3|Schizosacc... 26 5.3 SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos... 25 7.0 SPBC1604.12 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 25 9.3 >SPAP8A3.09c |paa1||protein phosphatase regulatory subunit Paa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 590 Score = 95.1 bits (226), Expect = 8e-21 Identities = 50/93 (53%), Positives = 59/93 (63%), Gaps = 1/93 (1%) Frame = +1 Query: 1 AAVEETVVRDKAVASLRAVAEHHSPQALEEHFVPLVQRLAGGDWFTSRTSACGLFSVCYP 180 AA EETVVRDKAV SL V S + LE++FVPLVQRL+ +WFTSR S+ GL+ Y Sbjct: 97 AATEETVVRDKAVDSLNKVCICLSQEQLEQYFVPLVQRLSTAEWFTSRASSAGLYCAAYS 156 Query: 181 RVSAP-VKAELRQHFRSLCQDDTPMVAALLPTN 276 + P VK LRQ F LC D+ PMV TN Sbjct: 157 QSENPAVKVSLRQSFSHLCHDEAPMVRRPAATN 189 Score = 50.4 bits (115), Expect = 2e-07 Identities = 28/86 (32%), Positives = 43/86 (50%), Gaps = 1/86 (1%) Frame = +3 Query: 255 RRAAAYKLGEFAKVVEIEYVKSDLIPIFVILAEDDQDSVR-XXXXXXXXXXXXXXTPEDM 431 RR AA +F +V + + IP+F L+ DDQDSVR + ++ Sbjct: 183 RRPAATNCAKFVFLVTKQEAIDEFIPLFNSLSNDDQDSVRLLSFDIMVSLAEVLKSDSEI 242 Query: 432 EQHVMPTVRALSGDASWRVRYMVADN 509 +++ +R+ D+SWR RYMVA N Sbjct: 243 RHYLLQPLRSFVSDSSWRTRYMVAAN 268 Score = 32.3 bits (70), Expect = 0.061 Identities = 21/85 (24%), Positives = 35/85 (41%) Frame = +3 Query: 255 RRAAAYKLGEFAKVVEIEYVKSDLIPIFVILAEDDQDSVRXXXXXXXXXXXXXXTPEDME 434 R A + AKVV +K +LI FV+L +D + VR + Sbjct: 262 RYMVAANFVKLAKVVGPSLIKDELIKPFVLLMKDTEQEVRRAIATQIPGFCELLDKRIVL 321 Query: 435 QHVMPTVRALSGDASWRVRYMVADN 509 + ++P ++ L D + VR + N Sbjct: 322 EEIIPVIQELINDPAQHVRAALGMN 346 Score = 29.9 bits (64), Expect = 0.33 Identities = 17/34 (50%), Positives = 19/34 (55%) Frame = +3 Query: 273 KLGEFAKVVEIEYVKSDLIPIFVILAEDDQDSVR 374 KL KVV IE + L+P V LAED Q VR Sbjct: 385 KLEVVNKVVGIELLSQSLLPAIVTLAEDKQWRVR 418 >SPAC1952.14c |mrpl25||mitochondrial ribosomal protein subunit L25|Schizosaccharomyces pombe|chr 1|||Manual Length = 148 Score = 28.7 bits (61), Expect = 0.75 Identities = 16/58 (27%), Positives = 32/58 (55%), Gaps = 3/58 (5%) Frame = -2 Query: 369 RNLDHPLPKSQIWE*DRS*HIQSPQLSQTLQVCRQQRG---DHRSIILTEGAEMLTKF 205 +NLD+ LP+ W+ D + HI L+ ++ ++R ++R + L+E + +KF Sbjct: 72 QNLDNLLPQQMSWQKDSTRHILKGLLNPKGKISERKRDEILENRRLKLSESLKKTSKF 129 >SPBP8B7.21 |ubp3||ubiquitin C-terminal hydrolase Ubp3|Schizosaccharomyces pombe|chr 2|||Manual Length = 512 Score = 25.8 bits (54), Expect = 5.3 Identities = 12/30 (40%), Positives = 14/30 (46%) Frame = +1 Query: 46 LRAVAEHHSPQALEEHFVPLVQRLAGGDWF 135 L AV HH A H+ VQ+L WF Sbjct: 438 LNAVVYHHGTSASGGHYTVDVQQLDKSGWF 467 >SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 855 Score = 25.4 bits (53), Expect = 7.0 Identities = 13/33 (39%), Positives = 15/33 (45%) Frame = +3 Query: 54 RCRAPQPASTRGTLCATSTAPCWRRLVHFKNIC 152 R A P T T C T T P WRR + +C Sbjct: 625 RANATNPTPTC-TNCQTRTTPLWRRSPDGQPLC 656 >SPBC1604.12 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 860 Score = 25.0 bits (52), Expect = 9.3 Identities = 10/27 (37%), Positives = 14/27 (51%) Frame = -2 Query: 414 IEPQQPRRLRQPADERNLDHPLPKSQI 334 I+P PR P R+ HP+P Q+ Sbjct: 599 IDPSSPRDDSTPLSWRSHSHPIPPVQV 625 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,457,991 Number of Sequences: 5004 Number of extensions: 49250 Number of successful extensions: 138 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 125 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 135 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 287744314 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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