Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30592
(645 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 68 1e-12
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 64 2e-11
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 60 2e-10
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 44 2e-05
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 42 1e-04
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 40 3e-04
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 35 0.012
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 31 0.19
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 27 2.3
SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 27 2.3
SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst... 25 7.1
SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyce... 25 9.3
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 68.1 bits (159), Expect = 1e-12
Identities = 28/62 (45%), Positives = 46/62 (74%)
Frame = +1
Query: 313 DFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHL 492
+F++G + FDKD G I ELR++L++LGEKLS++E+++LL+G G +NY +FV +
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKDGMVNYHDFVQM 137
Query: 493 IM 498
I+
Sbjct: 138 IL 139
Score = 45.6 bits (103), Expect = 6e-06
Identities = 19/37 (51%), Positives = 26/37 (70%)
Frame = +2
Query: 98 FQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 208
+++AF LFD G G+I IGD LRA GQNPT +++
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI 44
Score = 27.5 bits (58), Expect = 1.8
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +2
Query: 95 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 208
EF + FQ+FD G I V ++ L +LG+ + ++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEM 115
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 64.1 bits (149), Expect = 2e-11
Identities = 30/71 (42%), Positives = 44/71 (61%), Gaps = 1/71 (1%)
Frame = +1
Query: 286 KARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLL-QGQEDSQG 462
K + D + E + FDKDGNG+I+ EL H+L++LGE+LS +EV ++ + D G
Sbjct: 77 KMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDG 136
Query: 463 NINYENFVHLI 495
INYE F +I
Sbjct: 137 VINYEEFSRVI 147
Score = 48.8 bits (111), Expect = 7e-07
Identities = 20/45 (44%), Positives = 34/45 (75%)
Frame = +2
Query: 77 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVK 211
+++Q+AEF+EAF LFD DG I ++G +R+LGQ+PT ++++
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQ 51
Score = 33.9 bits (74), Expect = 0.020
Identities = 17/62 (27%), Positives = 36/62 (58%), Gaps = 1/62 (1%)
Frame = +1
Query: 313 DFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLL-QGQEDSQGNINYENFVH 489
+F E FD+D +G I+S EL ++ +LG+ + E++ ++ + D G I++ F+
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLT 72
Query: 490 LI 495
++
Sbjct: 73 MM 74
Score = 33.5 bits (73), Expect = 0.027
Identities = 15/42 (35%), Positives = 26/42 (61%)
Frame = +2
Query: 83 DQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 208
D E +EAF++FD G+G I V ++ L +LG+ ++ +V
Sbjct: 82 DNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEV 123
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 60.5 bits (140), Expect = 2e-10
Identities = 28/73 (38%), Positives = 47/73 (64%), Gaps = 1/73 (1%)
Frame = +1
Query: 283 SKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQ-GQEDSQ 459
+K R ++ ++I+ R FDKD +G+I +A+ + TLGEKLSD+EV+ ++Q +
Sbjct: 69 NKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNS 128
Query: 460 GNINYENFVHLIM 498
G+ +Y +FV IM
Sbjct: 129 GSFDYYDFVQRIM 141
Score = 41.9 bits (94), Expect = 8e-05
Identities = 20/51 (39%), Positives = 32/51 (62%)
Frame = +2
Query: 77 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHL 229
S++Q E +EAF L+D DG I + +G LR+LG N T++++ K + L
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL 54
Score = 33.5 bits (73), Expect = 0.027
Identities = 15/53 (28%), Positives = 28/53 (52%)
Frame = +2
Query: 95 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISF 253
E+ +AF++FD G I A+ D ++ LG+ ++++V+ P SF
Sbjct: 79 EYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSF 131
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 43.6 bits (98), Expect = 2e-05
Identities = 26/68 (38%), Positives = 38/68 (55%)
Frame = -1
Query: 429 LYLIITELLSESREQVSQFRRRDEPIAIFVKMAQTLNKVISSVATACFRYGLVNWQKHPK 250
L+L+ L++ +Q + R + IAIF K + +K I SVA R+ L QK PK
Sbjct: 1377 LHLLFASLVAHKFDQPQHAQTRTKIIAIFFKDLYSPHKEIYSVAIDALRHVLSQNQKLPK 1436
Query: 249 DILSSGLR 226
++L SGLR
Sbjct: 1437 ELLQSGLR 1444
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 41.5 bits (93), Expect = 1e-04
Identities = 22/62 (35%), Positives = 33/62 (53%)
Frame = +1
Query: 310 NDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVH 489
ND +E FD +G I + +R LS++G+++ EVE +L+ S G YE FV
Sbjct: 117 NDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESILRSY-TSHGVFYYEKFVD 175
Query: 490 LI 495
I
Sbjct: 176 AI 177
Score = 33.9 bits (74), Expect = 0.020
Identities = 17/56 (30%), Positives = 26/56 (46%)
Frame = +2
Query: 68 AGYSEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKP 235
A + Q+ E +EAF L D GDG I + L +L Q+ +E + + P
Sbjct: 40 AQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESINP 95
Score = 29.9 bits (64), Expect = 0.33
Identities = 11/40 (27%), Positives = 23/40 (57%)
Frame = +1
Query: 322 EGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQ 441
E DKDG+G I +++ +L++L + S+D + + +
Sbjct: 52 EAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFE 91
Score = 29.5 bits (63), Expect = 0.43
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +2
Query: 104 EAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVK 211
EAF FD GKI ++ + DAL ++G +V+
Sbjct: 121 EAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVE 156
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 39.9 bits (89), Expect = 3e-04
Identities = 20/46 (43%), Positives = 29/46 (63%)
Frame = +2
Query: 77 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKK 214
+E+Q + EAF+LFDS D I ++ A+RALG N +S+V K
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLK 77
Score = 30.7 bits (66), Expect = 0.19
Identities = 18/64 (28%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
Frame = +1
Query: 313 DFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQG-QEDSQGNINYENFVH 489
D E + FD D + I ELR + LG EV ++L+ + +G + E+FV
Sbjct: 38 DINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVR 97
Query: 490 LIMQ 501
++ +
Sbjct: 98 VMTE 101
Score = 29.5 bits (63), Expect = 0.43
Identities = 13/43 (30%), Positives = 23/43 (53%)
Frame = +2
Query: 83 DQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVK 211
D L E + AF+LFD GKI + + + L +N + +++
Sbjct: 107 DPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELE 149
Score = 25.4 bits (53), Expect = 7.1
Identities = 14/59 (23%), Positives = 32/59 (54%), Gaps = 2/59 (3%)
Frame = +1
Query: 310 NDFIEGLRHFDKDGNGFISSAE-LRHLLSTLGEKLSDDEVEQLLQ-GQEDSQGNINYEN 480
++ ++ LR FDK G G++ + +R + + E+ +E+++ + +D G I+ N
Sbjct: 73 SEVLKILRDFDKTGKGYLQMEDFVRVMTEKIVERDPLEEIKRAFELFDDDETGKISLRN 131
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 34.7 bits (76), Expect = 0.012
Identities = 15/57 (26%), Positives = 28/57 (49%)
Frame = +1
Query: 331 RHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHLIMQ 501
RHF+K + ++ E L++LG +E L +S+ + YE F ++M+
Sbjct: 494 RHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEGVTYERFTEIVME 550
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 30.7 bits (66), Expect = 0.19
Identities = 16/52 (30%), Positives = 27/52 (51%)
Frame = +1
Query: 337 FDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHL 492
FD G+I +LR + LGE L+ +++ QL+ + G ++ E F L
Sbjct: 21 FDVTHKGYIDFEDLRRSCAQLGENLTKEQL-QLMLDLAGTNGKVSREEFAEL 71
Score = 25.8 bits (54), Expect = 5.3
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = +2
Query: 95 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVK 211
E +EAF LFD G I + + LG+N T+ ++
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQ 51
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 27.1 bits (57), Expect = 2.3
Identities = 16/48 (33%), Positives = 27/48 (56%)
Frame = +1
Query: 361 ISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHLIMQG 504
I A++ LLSTL E D ++EQ+ Q + + Y +F+ ++QG
Sbjct: 42 IVKAQILFLLSTLREDQYDTKLEQIRQLINKNAPRV-YHHFLRRLIQG 88
>SPAC1565.07c |||TATA binding protein interacting protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1220
Score = 27.1 bits (57), Expect = 2.3
Identities = 15/38 (39%), Positives = 24/38 (63%), Gaps = 2/38 (5%)
Frame = +1
Query: 331 RHFDKDGNGFISS-AELRHLLSTLGEK-LSDDEVEQLL 438
RH DK GN F ++ EL +L +G+K L++ +E +L
Sbjct: 670 RHIDKSGNEFTTAYLELLEVLLKVGQKYLAESLLEHIL 707
>SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit
Pst2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1075
Score = 25.4 bits (53), Expect = 7.1
Identities = 18/48 (37%), Positives = 25/48 (52%)
Frame = -1
Query: 498 HDEVNKVLIVDISLRVFLSLQELLYLIITELLSESREQVSQFRRRDEP 355
HD+V+K S+ VF L LLY + E L +QVS ++R P
Sbjct: 736 HDDVDKEQFGYSSMYVFFRLFNLLYERLYE-LQRLEDQVSIIQQRIIP 782
>SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1131
Score = 25.0 bits (52), Expect = 9.3
Identities = 8/14 (57%), Positives = 10/14 (71%)
Frame = +3
Query: 354 WVHLFCGTATPALY 395
WVHL C + TP +Y
Sbjct: 927 WVHLICASWTPDVY 940
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,339,936
Number of Sequences: 5004
Number of extensions: 46169
Number of successful extensions: 168
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 168
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 289756512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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