Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30561X
(508 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g25870.1 68418.m03069 hypothetical protein 32 0.19
At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata format... 31 0.34
At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata format... 31 0.34
At3g54670.1 68416.m06049 structural maintenance of chromosomes (... 31 0.59
At1g21630.1 68414.m02708 calcium-binding EF hand family protein ... 31 0.59
At5g10010.1 68418.m01159 expressed protein 30 0.78
At4g11990.1 68417.m01908 expressed protein hypothetical protein ... 29 1.4
At3g58940.1 68416.m06568 F-box family protein contains F-box dom... 29 1.4
At5g62030.1 68418.m07786 diphthamide synthesis DPH2 family prote... 29 1.8
At5g26350.1 68418.m03150 hypothetical protein 29 1.8
At3g58280.1 68416.m06497 hypothetical protein 29 1.8
At1g45545.1 68414.m05199 hypothetical protein contains Pfam prof... 29 1.8
At5g41540.1 68418.m05048 disease resistance protein (TIR-NBS-LRR... 29 2.4
At2g25350.1 68415.m03032 phox (PX) domain-containing protein wea... 29 2.4
At3g19040.1 68416.m02418 ubiquitin family protein / DNA-binding ... 28 3.1
At2g39300.1 68415.m04825 expressed protein ; expression supporte... 28 4.2
At1g60900.1 68414.m06856 U2 snRNP auxiliary factor large subunit... 28 4.2
At3g51150.1 68416.m05601 kinesin motor family protein contains P... 27 5.5
At1g48280.1 68414.m05393 hydroxyproline-rich glycoprotein family... 27 5.5
At1g14650.1 68414.m01741 SWAP (Suppressor-of-White-APricot)/surp... 27 5.5
At5g42880.1 68418.m05226 hypothetical protein contains Pfam prof... 27 7.3
At4g38070.1 68417.m05377 bHLH family protein contains Pfam profi... 27 7.3
At4g04720.1 68417.m00693 calcium-dependent protein kinase, putat... 27 7.3
At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains... 27 7.3
At1g12080.2 68414.m01397 expressed protein 27 7.3
At1g08730.1 68414.m00969 myosin heavy chain (PCR43) identical to... 27 7.3
At1g77580.2 68414.m09032 myosin heavy chain-related low similari... 27 9.6
At1g77580.1 68414.m09033 myosin heavy chain-related low similari... 27 9.6
At1g75260.1 68414.m08743 isoflavone reductase family protein sim... 27 9.6
>At5g25870.1 68418.m03069 hypothetical protein
Length = 173
Score = 32.3 bits (70), Expect = 0.19
Identities = 14/36 (38%), Positives = 24/36 (66%)
Frame = +3
Query: 318 KLRQKAQELWECIVKLETEKYDLEERQKRQDYDLKE 425
K + +E+ E +VKLE + Y+LEE + R++ +KE
Sbjct: 69 KEHDEFEEISEILVKLEKKYYELEEVENRENLQMKE 104
>At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 31.5 bits (68), Expect = 0.34
Identities = 13/27 (48%), Positives = 18/27 (66%)
Frame = -3
Query: 380 VFLGLEFDDALPEFLGLLSEFVDGETL 300
VF+ EFD+ LP++L L VD +TL
Sbjct: 423 VFISDEFDELLPKYLSFLKGLVDSDTL 449
>At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 31.5 bits (68), Expect = 0.34
Identities = 13/27 (48%), Positives = 18/27 (66%)
Frame = -3
Query: 380 VFLGLEFDDALPEFLGLLSEFVDGETL 300
VF+ EFD+ LP++L L VD +TL
Sbjct: 423 VFISDEFDELLPKYLSFLKGLVDSDTL 449
>At3g54670.1 68416.m06049 structural maintenance of chromosomes
(SMC) family protein similar to SMC1 protein [Bos
taurus] GI:4235253, 14S cohesin SMC1 subunit (SMC
protein) [Xenopus laevis] GI:3328231; contains Pfam
profiles PF02483: SMC family C-terminal domain, PF02463:
RecF/RecN/SMC N terminal domain
Length = 1257
Score = 30.7 bits (66), Expect = 0.59
Identities = 21/65 (32%), Positives = 36/65 (55%)
Frame = +3
Query: 252 REKKISLSIRIKPLTIEGLSVDKLRQKAQELWECIVKLETEKYDLEERQKRQDYDLKELE 431
REKKI+ K + + + LR K +E+ K+ET + D+++R+K + KE+E
Sbjct: 290 REKKIAE----KSSKLGKIQPELLRFK-EEIARIKAKIETNRKDVDKRKKEKGKHSKEIE 344
Query: 432 ERQSS 446
+ Q S
Sbjct: 345 QMQKS 349
>At1g21630.1 68414.m02708 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain; ESTs gb|T44428 and gb|AA395440 come from this
gene
Length = 1218
Score = 30.7 bits (66), Expect = 0.59
Identities = 17/48 (35%), Positives = 27/48 (56%)
Frame = +3
Query: 291 LTIEGLSVDKLRQKAQELWECIVKLETEKYDLEERQKRQDYDLKELEE 434
LTIE + +++K EL++ IVK E K D ++R ++ LEE
Sbjct: 678 LTIEEATFRDIQEKKMELYQAIVKFEEGKLDDSIVKERTEHIQSGLEE 725
>At5g10010.1 68418.m01159 expressed protein
Length = 434
Score = 30.3 bits (65), Expect = 0.78
Identities = 27/117 (23%), Positives = 50/117 (42%), Gaps = 1/117 (0%)
Frame = +3
Query: 150 CQQDRTQLHHPKEERKLRFEQCPXXXXXXXXXXXREKKISLSIRIKPLTIEGLSVDKLRQ 329
C Q R+ L H KE++ +FE C E+ + I E V +
Sbjct: 277 CTQRRSALRHMKEDQLKKFEYCLPYFYQPFKEDELEQSTEVQIMFPS---EPPVVCEFDW 333
Query: 330 KAQELWECIVKL-ETEKYDLEERQKRQDYDLKELEERQSSN*GTKLSRRVSTPKRSQ 497
+ EL E + KL E E E+ + ++Y +++ + +N K +R+ + + S+
Sbjct: 334 EFDELQEFVDKLVEEEALPAEQADEFKEYVKEQVRAAKKANREAKDARKKAIEEMSE 390
>At4g11990.1 68417.m01908 expressed protein hypothetical protein
F7H19.40 - Arabidopsis thaliana, PID:e1310054
Length = 501
Score = 29.5 bits (63), Expect = 1.4
Identities = 18/41 (43%), Positives = 24/41 (58%), Gaps = 1/41 (2%)
Frame = +2
Query: 374 EIRS-RREAKETGLRLKRARRKTKQQLRHKALKKGLDPEAL 493
EI+S +R+ + GL K A + L HKALKK LD +L
Sbjct: 200 EIQSAKRQKLDGGLLRKVAEAAQETNLVHKALKKDLDKNSL 240
>At3g58940.1 68416.m06568 F-box family protein contains F-box domain
Pfam:PF00646
Length = 618
Score = 29.5 bits (63), Expect = 1.4
Identities = 13/34 (38%), Positives = 24/34 (70%)
Frame = +3
Query: 336 QELWECIVKLETEKYDLEERQKRQDYDLKELEER 437
QE+ + ++++ E DL+ERQ+ Q+ L +L+ER
Sbjct: 486 QEMSQDYLQMQLEVADLKERQRDQEAKLADLKER 519
>At5g62030.1 68418.m07786 diphthamide synthesis DPH2 family protein
contains InterPro accession IPR002728: Diphthamide
synthesis DPH2 protein
Length = 453
Score = 29.1 bits (62), Expect = 1.8
Identities = 17/46 (36%), Positives = 26/46 (56%), Gaps = 4/46 (8%)
Frame = +2
Query: 356 RQTRDREIRSRREAKETGLRL----KRARRKTKQQLRHKALKKGLD 481
R+TR R I REAK G+ L ++ K ++L K ++KG+D
Sbjct: 268 RETRMRAIARAREAKTWGIVLGTLGRQGNPKILERLEKKMMEKGID 313
>At5g26350.1 68418.m03150 hypothetical protein
Length = 126
Score = 29.1 bits (62), Expect = 1.8
Identities = 19/54 (35%), Positives = 32/54 (59%), Gaps = 1/54 (1%)
Frame = +3
Query: 318 KLRQKAQELWECIVKLETEKYDLEERQKRQDYDLKELE-ERQSSN*GTKLSRRV 476
K +++ +L E KLE EK LEE +K+ + + K+LE E +N K+ R++
Sbjct: 52 KHKEEKNKLEEEKKKLEKEKKQLEEEKKQLEEEKKQLEFEVMGANEREKVLRQL 105
Score = 27.9 bits (59), Expect = 4.2
Identities = 13/42 (30%), Positives = 27/42 (64%)
Frame = +3
Query: 315 DKLRQKAQELWECIVKLETEKYDLEERQKRQDYDLKELEERQ 440
+KL ++ ++L + +LE EK LEE +K+ ++++ ER+
Sbjct: 58 NKLEEEKKKLEKEKKQLEEEKKQLEEEKKQLEFEVMGANERE 99
>At3g58280.1 68416.m06497 hypothetical protein
Length = 196
Score = 29.1 bits (62), Expect = 1.8
Identities = 17/50 (34%), Positives = 28/50 (56%)
Frame = +3
Query: 282 IKPLTIEGLSVDKLRQKAQELWECIVKLETEKYDLEERQKRQDYDLKELE 431
I L GL++D LRQK + E + +T +LE++ K++ + ELE
Sbjct: 129 ITDLIKAGLNLDWLRQKFDQALEKQIAYDTRIGELEKQVKKRKLAVTELE 178
>At1g45545.1 68414.m05199 hypothetical protein contains Pfam profile
PF05701: Plant protein of unknown function (DUF827)
Length = 752
Score = 29.1 bits (62), Expect = 1.8
Identities = 13/24 (54%), Positives = 18/24 (75%)
Frame = +3
Query: 357 VKLETEKYDLEERQKRQDYDLKEL 428
+KLE EK + EE+Q +QD DL +L
Sbjct: 233 LKLELEKAEKEEQQAKQDSDLAKL 256
>At5g41540.1 68418.m05048 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1038
Score = 28.7 bits (61), Expect = 2.4
Identities = 19/50 (38%), Positives = 24/50 (48%)
Frame = +1
Query: 76 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLE 225
D EKRQ L EAE+ R + S G +F + K SE F +S E
Sbjct: 502 DEPEKRQFLVEAEEIRDVLANETGTGSVLGISFDMSKVSE-FSISGRAFE 550
>At2g25350.1 68415.m03032 phox (PX) domain-containing protein weak
similarity to SP|Q9UTK5 Abnormal long morphology protein
1 (Sp8) {Schizosaccharomyces pombe}; contains Pfam
profile PF00787: PX domain
Length = 643
Score = 28.7 bits (61), Expect = 2.4
Identities = 22/60 (36%), Positives = 30/60 (50%)
Frame = +1
Query: 76 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEE 255
D+EE RQ+ E E K ++ D SKT N TI + E E + TK+QLE+
Sbjct: 455 DMEELRQKTFEMELKLKSKEDGSSD-SKTSGNSTISESHE------LLQEMDATKQQLED 507
>At3g19040.1 68416.m02418 ubiquitin family protein / DNA-binding
bromodomain-containing protein low similarity to
SP|P51123 Transcription initiation factor TFIID 230 kDa
subunit {Drosophila melanogaster}; contains Pfam
profiles: PF00439 bromodomain, PF00240: Ubiquitin family
Length = 1700
Score = 28.3 bits (60), Expect = 3.1
Identities = 20/74 (27%), Positives = 36/74 (48%)
Frame = +2
Query: 248 WKREEDLPVHPHQAADHRGSLRRQTPTEGPGTLGVHRQTRDREIRSRREAKETGLRLKRA 427
W++E+D+ H+ R + T +E + R+ R+ IRS RE + KR
Sbjct: 1466 WRKEQDISTELHREGKVRRAYDDSTVSEERSEIAESRRYRE-VIRSEREEE------KRR 1518
Query: 428 RRKTKQQLRHKALK 469
+ K K++L+ L+
Sbjct: 1519 KAKQKKKLQRGILE 1532
>At2g39300.1 68415.m04825 expressed protein ; expression supported
by MPSS
Length = 768
Score = 27.9 bits (59), Expect = 4.2
Identities = 12/55 (21%), Positives = 30/55 (54%)
Frame = +3
Query: 270 LSIRIKPLTIEGLSVDKLRQKAQELWECIVKLETEKYDLEERQKRQDYDLKELEE 434
+ I ++ E L++ K+ ++ +E+W+ + + DLE ++ +++LEE
Sbjct: 676 MEINLQEAAKELLTLPKVLEEREEMWKEVKECRKRNMDLESEKEMLKKKVEKLEE 730
>At1g60900.1 68414.m06856 U2 snRNP auxiliary factor large subunit,
putative similar to U2 snRNP auxiliary factor, large
subunit GB:CAA77136 from [Nicotiana plumbaginifolia]
Length = 589
Score = 27.9 bits (59), Expect = 4.2
Identities = 12/33 (36%), Positives = 21/33 (63%)
Frame = +2
Query: 365 RDREIRSRREAKETGLRLKRARRKTKQQLRHKA 463
RDRE+R RR ++ R+ R+++ + RHK+
Sbjct: 141 RDREVRHRRRSRSRSR--SRSERRSRSEHRHKS 171
>At3g51150.1 68416.m05601 kinesin motor family protein contains Pfam
domain, PF00225: Kinesin motor domain
Length = 1025
Score = 27.5 bits (58), Expect = 5.5
Identities = 13/35 (37%), Positives = 22/35 (62%)
Frame = +3
Query: 252 REKKISLSIRIKPLTIEGLSVDKLRQKAQELWECI 356
RE+KI +S+R++PL + ++ R + WECI
Sbjct: 14 REEKIFVSVRLRPLNVR----ERARNDVAD-WECI 43
>At1g48280.1 68414.m05393 hydroxyproline-rich glycoprotein family
protein
Length = 558
Score = 27.5 bits (58), Expect = 5.5
Identities = 18/53 (33%), Positives = 27/53 (50%)
Frame = +1
Query: 82 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTK 240
+EKR+R+EE E+K +KD N ++ + E SN +LE N K
Sbjct: 112 DEKRKRMEELEEKLVVNESLIKDLQLQVLN--LKTELEEARNSNVELELNNRK 162
>At1g14650.1 68414.m01741 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein / ubiquitin family protein
similar to SP|Q15459 Splicing factor 3 subunit 1
(Spliceosome associated protein 114) {Homo sapiens};
contains Pfam profiles PF00240: Ubiquitin family,
PF01805: Surp module
Length = 785
Score = 27.5 bits (58), Expect = 5.5
Identities = 17/46 (36%), Positives = 29/46 (63%), Gaps = 4/46 (8%)
Frame = +3
Query: 315 DKLRQKAQELW----ECIVKLETEKYDLEERQKRQDYDLKELEERQ 440
+KLR+ A +L C+ +LE ++ +E+QK+++ D KELE Q
Sbjct: 254 EKLRKSAADLTTVLERCLHRLEWDRS--QEQQKKKEEDEKELERVQ 297
>At5g42880.1 68418.m05226 hypothetical protein contains Pfam profile
PF05701: Plant protein of unknown function (DUF827)
Length = 751
Score = 27.1 bits (57), Expect = 7.3
Identities = 11/24 (45%), Positives = 18/24 (75%)
Frame = +3
Query: 357 VKLETEKYDLEERQKRQDYDLKEL 428
+KLE EK + EE+Q +QD +L ++
Sbjct: 212 LKLELEKAEKEEQQAKQDSELAQM 235
>At4g38070.1 68417.m05377 bHLH family protein contains Pfam profile:
PF00010 helix-loop-helix DNA-binding domain; PMID:
12679534; putative bHLH131 transcription factor
Length = 1513
Score = 27.1 bits (57), Expect = 7.3
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = +3
Query: 348 ECIVKLETEKYDLEERQKRQDYDLKELEE 434
+ +VK+E EK +EE+ K + K LEE
Sbjct: 170 DVVVKMEEEKSQVEEKLKWKKEQFKHLEE 198
>At4g04720.1 68417.m00693 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase(CDPK) [Carrot] SWISS-PROT:P28582
Length = 531
Score = 27.1 bits (57), Expect = 7.3
Identities = 14/38 (36%), Positives = 22/38 (57%), Gaps = 3/38 (7%)
Frame = +2
Query: 257 EEDLPVHP---HQAADHRGSLRRQTPTEGPGTLGVHRQ 361
E+ +P++P H +HR + QTPT P T +H+Q
Sbjct: 17 EKSIPINPVQTHVVPEHR---KPQTPTPKPMTQPIHQQ 51
>At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains
ATP/GTP-binding site motif A (P-loop), PROSITE:PS00017
Length = 1340
Score = 27.1 bits (57), Expect = 7.3
Identities = 14/57 (24%), Positives = 31/57 (54%), Gaps = 1/57 (1%)
Frame = +1
Query: 79 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNA-QLERNKTKEQ 246
++ K++ KK++ + K +KTG + +KK+ + +S +++RN T E+
Sbjct: 898 VDVKQETGSPDTKKKEGASSSSKKDTKTGEDKKAEKKNNSETMSEGKKIDRNNTDEK 954
>At1g12080.2 68414.m01397 expressed protein
Length = 138
Score = 27.1 bits (57), Expect = 7.3
Identities = 17/59 (28%), Positives = 30/59 (50%)
Frame = +1
Query: 79 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEE 255
+EE ++ EE EKK + + + ++T +KK+E + A +E K E +EE
Sbjct: 62 VEEAEKKDEETEKKTEEKDEKTEVITETPVVEEEEKKAEEVTETPAVVEEEKKTEVVEE 120
>At1g08730.1 68414.m00969 myosin heavy chain (PCR43) identical to
myosin heavy chain PCR43 (PIR:T00727) [Arabidopsis
thaliana]; similar to ESTs gb|R30087 and gb|AA394762
Length = 1538
Score = 27.1 bits (57), Expect = 7.3
Identities = 11/37 (29%), Positives = 22/37 (59%)
Frame = +1
Query: 79 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKK 189
+E+K+++LEE EKK Q + +++ + N + K
Sbjct: 1018 LEDKKKKLEETEKKGQQLQESLTRMEEKCSNLESENK 1054
>At1g77580.2 68414.m09032 myosin heavy chain-related low similarity
to SP|P08799 Myosin II heavy chain, non muscle
{Dictyostelium discoideum}
Length = 779
Score = 26.6 bits (56), Expect = 9.6
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +3
Query: 312 VDKLRQKAQELWECIVKLETEKYDLEERQK 401
++ L + +EL E + KLE EK++LE K
Sbjct: 345 IEVLTSRIKELEEKLEKLEAEKHELENEVK 374
>At1g77580.1 68414.m09033 myosin heavy chain-related low similarity
to SP|P08799 Myosin II heavy chain, non muscle
{Dictyostelium discoideum}
Length = 629
Score = 26.6 bits (56), Expect = 9.6
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +3
Query: 312 VDKLRQKAQELWECIVKLETEKYDLEERQK 401
++ L + +EL E + KLE EK++LE K
Sbjct: 311 IEVLTSRIKELEEKLEKLEAEKHELENEVK 340
>At1g75260.1 68414.m08743 isoflavone reductase family protein
similar to SP|P52577 Isoflavone reductase homolog P3 (EC
1.3.1.-) {Arabidopsis thaliana}; contains Pfam profile
PF02716: Isoflavone reductase
Length = 593
Score = 26.6 bits (56), Expect = 9.6
Identities = 26/97 (26%), Positives = 42/97 (43%), Gaps = 1/97 (1%)
Frame = +2
Query: 161 PDPTSPSKRRAKTSV*AMPSWSATRPRSSWKREEDLPVHPHQAADHRGSLRRQTPTEGPG 340
P PT PS+ + + A PS S T+P++S + AA S Q P+ G
Sbjct: 28 PPPTPPSRPPFRPAGIAQPSKSETKPKASPSLSRS---RSNVAALAASSSASQLPSLGAA 84
Query: 341 T-LGVHRQTRDREIRSRREAKETGLRLKRARRKTKQQ 448
T + +QT + K LR++ + TK++
Sbjct: 85 TPTRLAKQTNQQS--GSPSKKLDSLRMEEQKVATKEK 119
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.300 0.115 0.292
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,740,439
Number of Sequences: 28952
Number of extensions: 134485
Number of successful extensions: 514
Number of sequences better than 10.0: 29
Number of HSP's better than 10.0 without gapping: 486
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 512
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 908059136
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 17 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 43 (21.7 bits)
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