Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30538
(392 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70... 76 1e-14
At5g02500.1 68418.m00183 heat shock cognate 70 kDa protein 1 (HS... 72 2e-13
At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3 (HS... 71 2e-13
At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2 (HS... 71 3e-13
At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70... 70 5e-13
At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein, puta... 67 5e-12
At5g42020.2 68418.m05116 luminal binding protein 2 (BiP-2) (BP2)... 60 4e-10
At5g42020.1 68418.m05115 luminal binding protein 2 (BiP-2) (BP2)... 60 4e-10
At5g28540.1 68418.m03480 luminal binding protein 1 (BiP-1) (BP1)... 60 4e-10
At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)... 55 1e-08
At1g14650.1 68414.m01741 SWAP (Suppressor-of-White-APricot)/surp... 32 0.16
At1g14640.1 68414.m01740 SWAP (Suppressor-of-White-APricot)/surp... 31 0.37
At5g01570.1 68418.m00072 hypothetical protein hypothetical prote... 29 1.1
At3g56850.1 68416.m06322 ABA-responsive element-binding protein ... 29 1.1
At1g11730.1 68414.m01346 galactosyltransferase family protein co... 28 2.0
At5g60030.1 68418.m07527 expressed protein 28 2.6
At2g44710.1 68415.m05564 RNA recognition motif (RRM)-containing ... 28 2.6
At2g01270.1 68415.m00040 thioredoxin family protein low similari... 27 3.4
At2g41070.3 68415.m05073 basic leucine zipper transcription fact... 27 4.5
At2g41070.2 68415.m05072 basic leucine zipper transcription fact... 27 4.5
At2g41070.1 68415.m05071 basic leucine zipper transcription fact... 27 4.5
At1g79930.1 68414.m09340 heat shock protein, putative contains P... 27 4.5
At3g60000.1 68416.m06699 hypothetical protein contains Pfam pro... 26 7.9
At3g19050.1 68416.m02420 kinesin motor protein-related contains ... 26 7.9
At1g44910.1 68414.m05146 FF domain-containing protein / WW domai... 26 7.9
>At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70,
putative similar to heat shock protein hsp70 GI:1771478
from [Pisum sativum]
Length = 646
Score = 75.8 bits (178), Expect = 1e-14
Identities = 33/58 (56%), Positives = 45/58 (77%)
Frame = +2
Query: 2 DEKLKEKISDSDKQTILDKCNNTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 175
DEKL +K++ DKQ I + TI+W++ NQLA+ +E+E+K KELEGI NPII+KMYQ
Sbjct: 560 DEKLAQKLTQEDKQKIEKAIDETIEWIEGNQLAEVDEFEYKLKELEGICNPIISKMYQ 617
>At5g02500.1 68418.m00183 heat shock cognate 70 kDa protein 1
(HSC70-1) (HSP70-1) identical to SP|P22953 Heat shock
cognate 70 kDa protein 1 (Hsc70.1) {Arabidopsis
thaliana}
Length = 651
Score = 71.7 bits (168), Expect = 2e-13
Identities = 32/58 (55%), Positives = 41/58 (70%)
Frame = +2
Query: 2 DEKLKEKISDSDKQTILDKCNNTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 175
DEK+ EK+ +DK+ I D I+WL+ NQLA+ +E+E K KELE I NPII KMYQ
Sbjct: 561 DEKIGEKLPAADKKKIEDSIEQAIQWLEGNQLAEADEFEDKMKELESICNPIIAKMYQ 618
>At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3
(HSC70-3) (HSP70-3) identical to SP|O65719 Heat shock
cognate 70 kDa protein 3 (Hsc70.3) {Arabidopsis
thaliana}
Length = 649
Score = 71.3 bits (167), Expect = 2e-13
Identities = 32/58 (55%), Positives = 42/58 (72%)
Frame = +2
Query: 2 DEKLKEKISDSDKQTILDKCNNTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 175
DEK+ EK++ DK+ I D I+WL++NQLA+ +E+E K KELE I NPII KMYQ
Sbjct: 561 DEKIGEKLAGDDKKKIEDSIEAAIEWLEANQLAECDEFEDKMKELESICNPIIAKMYQ 618
>At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2
(HSC70-2) (HSP70-2) identical to SP|P22954 Heat shock
cognate 70 kDa protein 2 (Hsc70.2) {Arabidopsis
thaliana}
Length = 653
Score = 70.9 bits (166), Expect = 3e-13
Identities = 30/58 (51%), Positives = 40/58 (68%)
Frame = +2
Query: 2 DEKLKEKISDSDKQTILDKCNNTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 175
DEK+ EK+ +DK+ + D I+WLD NQL + +E+E K KELE + NPII KMYQ
Sbjct: 561 DEKIGEKLPAADKKKVEDSIEEAIQWLDGNQLGEADEFEDKMKELESVCNPIIAKMYQ 618
>At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70,
putative strong similarity to heat shock protein
GI:425194 [Spinacia oleracea]
Length = 650
Score = 70.1 bits (164), Expect = 5e-13
Identities = 30/58 (51%), Positives = 41/58 (70%)
Frame = +2
Query: 2 DEKLKEKISDSDKQTILDKCNNTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 175
DEK+ K+ +DK+ I D + I+WLD NQLA+ +E+E K KELE + NPII +MYQ
Sbjct: 561 DEKIASKLDAADKKKIEDAIDQAIEWLDGNQLAEADEFEDKMKELESLCNPIIARMYQ 618
>At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein,
putative / HSC70, putative / HSP70, putative strong
similarity to heat shock cognate 70 kd protein 1
SP:P22953 [Arabidopsis thaliana (Mouse-ear cress)]
Length = 617
Score = 66.9 bits (156), Expect = 5e-12
Identities = 28/53 (52%), Positives = 38/53 (71%)
Frame = +2
Query: 17 EKISDSDKQTILDKCNNTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 175
EK+ +DK+ D I+WLD NQLA+ +E+EHK KELE +++ IITKMYQ
Sbjct: 564 EKLPAADKKKFEDSIEEVIQWLDDNQLAEADEFEHKMKELESVWSTIITKMYQ 616
>At5g42020.2 68418.m05116 luminal binding protein 2 (BiP-2) (BP2)
similar to SWISS-PROT: Q39043; GI:1303695; luminal
binding protein (BiP) [Arabidopsis thaliana]
Length = 613
Score = 60.5 bits (140), Expect = 4e-10
Identities = 25/57 (43%), Positives = 38/57 (66%)
Frame = +2
Query: 5 EKLKEKISDSDKQTILDKCNNTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 175
+KL +K+ +K+ I ++WLD NQ ++KEEY+ K KE+E + NPIIT +YQ
Sbjct: 533 DKLADKLEGDEKEKIEAATKEALEWLDENQNSEKEEYDEKLKEVEAVCNPIITAVYQ 589
>At5g42020.1 68418.m05115 luminal binding protein 2 (BiP-2) (BP2)
similar to SWISS-PROT: Q39043; GI:1303695; luminal
binding protein (BiP) [Arabidopsis thaliana]
Length = 668
Score = 60.5 bits (140), Expect = 4e-10
Identities = 25/57 (43%), Positives = 38/57 (66%)
Frame = +2
Query: 5 EKLKEKISDSDKQTILDKCNNTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 175
+KL +K+ +K+ I ++WLD NQ ++KEEY+ K KE+E + NPIIT +YQ
Sbjct: 588 DKLADKLEGDEKEKIEAATKEALEWLDENQNSEKEEYDEKLKEVEAVCNPIITAVYQ 644
>At5g28540.1 68418.m03480 luminal binding protein 1 (BiP-1) (BP1)
SWISS-PROT:Q9LKR3 PMID:8888624
Length = 669
Score = 60.5 bits (140), Expect = 4e-10
Identities = 25/57 (43%), Positives = 38/57 (66%)
Frame = +2
Query: 5 EKLKEKISDSDKQTILDKCNNTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 175
+KL +K+ +K+ I ++WLD NQ ++KEEY+ K KE+E + NPIIT +YQ
Sbjct: 588 DKLADKLEGDEKEKIEAATKEALEWLDENQNSEKEEYDEKLKEVEAVCNPIITAVYQ 644
>At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)
Similar to Arabidopsis luminal binding protein
(gb|D89342); contains Pfam domain PF00012: dnaK protein
Length = 678
Score = 55.2 bits (127), Expect = 1e-08
Identities = 23/57 (40%), Positives = 38/57 (66%)
Frame = +2
Query: 5 EKLKEKISDSDKQTILDKCNNTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 175
EKL +KISD DK+ + ++WL+ N A+KE+Y+ K KE+E + +P+I +Y+
Sbjct: 602 EKLAKKISDEDKEKMEGVLKEALEWLEENVNAEKEDYDEKLKEVELVCDPVIKSVYE 658
>At1g14650.1 68414.m01741 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein / ubiquitin family protein
similar to SP|Q15459 Splicing factor 3 subunit 1
(Spliceosome associated protein 114) {Homo sapiens};
contains Pfam profiles PF00240: Ubiquitin family,
PF01805: Surp module
Length = 785
Score = 31.9 bits (69), Expect = 0.16
Identities = 19/52 (36%), Positives = 33/52 (63%), Gaps = 2/52 (3%)
Frame = +2
Query: 11 LKEKI--SDSDKQTILDKCNNTIKWLDSNQLADKEEYEHKQKELEGIYNPII 160
LKEK+ S +D T+L++C + ++W D +Q K++ E +KELE + +I
Sbjct: 252 LKEKLRKSAADLTTVLERCLHRLEW-DRSQEQQKKK-EEDEKELERVQMAMI 301
>At1g14640.1 68414.m01740 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein similar to human splicing
factor GB:CAA59494 GI:899298 from [Homo sapiens];
contains Pfam profile PF01805: Surp module
Length = 735
Score = 30.7 bits (66), Expect = 0.37
Identities = 15/43 (34%), Positives = 25/43 (58%), Gaps = 2/43 (4%)
Frame = +2
Query: 11 LKEKISDS--DKQTILDKCNNTIKWLDSNQLADKEEYEHKQKE 133
LKEK+ S D T+L++C N ++W + +E + K+KE
Sbjct: 243 LKEKLRKSVADLTTVLERCLNRLEWDRFQEEEKNKEEDEKEKE 285
>At5g01570.1 68418.m00072 hypothetical protein hypothetical protein
T16O11.19 - Arabidopsis thaliana, EMBL:AC010871
Length = 157
Score = 29.1 bits (62), Expect = 1.1
Identities = 21/48 (43%), Positives = 27/48 (56%), Gaps = 2/48 (4%)
Frame = +2
Query: 2 DEKLKEKISDS--DKQTILDKCNNTIKWLDSNQLADKEEYEHKQKELE 139
DE KE I +S D Q LD C +S ++AD+EE E QKEL+
Sbjct: 46 DEDFKE-IHESLQDLQKKLDVCKEKTDEANS-EIADEEEIERLQKELD 91
>At3g56850.1 68416.m06322 ABA-responsive element-binding protein 3
(AREB3) identical to ABA-responsive element binding
protein 3 (AREB3) [Arabidopsis thaliana] GI:9967421
Length = 297
Score = 29.1 bits (62), Expect = 1.1
Identities = 16/52 (30%), Positives = 26/52 (50%)
Frame = +3
Query: 105 RRSMSTSRKNWKAFTIR*LRRCTRVPEESPEVCRASRAEHPEPEVPPPGLRR 260
R S + SR +A+T + +R+ EE+ + + E P VPPP +R
Sbjct: 236 RESAARSRARKQAYTHELEIKVSRLEEENERLRKQKEVEKILPSVPPPDPKR 287
>At1g11730.1 68414.m01346 galactosyltransferase family protein
contains Pfam profile: PF01762 galactosyltransferase
Length = 384
Score = 28.3 bits (60), Expect = 2.0
Identities = 14/28 (50%), Positives = 17/28 (60%)
Frame = +2
Query: 8 KLKEKISDSDKQTILDKCNNTIKWLDSN 91
KL SD DK+ +LD NNTI LD +
Sbjct: 55 KLSLSSSDCDKKNVLDYGNNTIGILDKS 82
>At5g60030.1 68418.m07527 expressed protein
Length = 292
Score = 27.9 bits (59), Expect = 2.6
Identities = 17/48 (35%), Positives = 25/48 (52%), Gaps = 2/48 (4%)
Frame = +2
Query: 2 DEKLKEKISDSDKQTILD--KCNNTIKWLDSNQLADKEEYEHKQKELE 139
DEK+KEK+ D K K + K D + + +KE+ E +QK E
Sbjct: 166 DEKVKEKLEDEQKSADRKERKKKKSKKNNDEDVVDEKEKLEDEQKSAE 213
>At2g44710.1 68415.m05564 RNA recognition motif (RRM)-containing
protein
Length = 809
Score = 27.9 bits (59), Expect = 2.6
Identities = 11/30 (36%), Positives = 14/30 (46%)
Frame = +3
Query: 171 TRVPEESPEVCRASRAEHPEPEVPPPGLRR 260
+R P S + SR P P +PPP R
Sbjct: 521 SRAPSSSAKRASGSRGRRPRPPLPPPARAR 550
>At2g01270.1 68415.m00040 thioredoxin family protein low similarity
to quiescin [Homo sapiens] GI:13257405; contains Pfam
profiles PF00085: Thioredoxin, PF04777: Erv1 / Alr
family
Length = 495
Score = 27.5 bits (58), Expect = 3.4
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = +2
Query: 77 WLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 175
+L SNQ + + ++EH K L+ Y P + +Y+
Sbjct: 402 YLSSNQKSIEWDHEHVYKFLKNYYGPKLVSLYK 434
>At2g41070.3 68415.m05073 basic leucine zipper transcription factor
(BZIP12) nearly identical to basic leucine zipper
transcription factor [Arabidopsis thaliana] GI:21694632;
contains a bZIP transcription factor basic domain
signature (PDOC00036)
Length = 262
Score = 27.1 bits (57), Expect = 4.5
Identities = 15/48 (31%), Positives = 24/48 (50%)
Frame = +3
Query: 105 RRSMSTSRKNWKAFTIR*LRRCTRVPEESPEVCRASRAEHPEPEVPPP 248
R S + SR +A+T + +R+ EE+ ++ R E P PPP
Sbjct: 201 RESAARSRARKQAYTHELEIKVSRLEEENEKLRRLKEVEKILPSEPPP 248
>At2g41070.2 68415.m05072 basic leucine zipper transcription factor
(BZIP12) nearly identical to basic leucine zipper
transcription factor [Arabidopsis thaliana] GI:21694632;
contains a bZIP transcription factor basic domain
signature (PDOC00036)
Length = 262
Score = 27.1 bits (57), Expect = 4.5
Identities = 15/48 (31%), Positives = 24/48 (50%)
Frame = +3
Query: 105 RRSMSTSRKNWKAFTIR*LRRCTRVPEESPEVCRASRAEHPEPEVPPP 248
R S + SR +A+T + +R+ EE+ ++ R E P PPP
Sbjct: 201 RESAARSRARKQAYTHELEIKVSRLEEENEKLRRLKEVEKILPSEPPP 248
>At2g41070.1 68415.m05071 basic leucine zipper transcription factor
(BZIP12) nearly identical to basic leucine zipper
transcription factor [Arabidopsis thaliana] GI:21694632;
contains a bZIP transcription factor basic domain
signature (PDOC00036)
Length = 262
Score = 27.1 bits (57), Expect = 4.5
Identities = 15/48 (31%), Positives = 24/48 (50%)
Frame = +3
Query: 105 RRSMSTSRKNWKAFTIR*LRRCTRVPEESPEVCRASRAEHPEPEVPPP 248
R S + SR +A+T + +R+ EE+ ++ R E P PPP
Sbjct: 201 RESAARSRARKQAYTHELEIKVSRLEEENEKLRRLKEVEKILPSEPPP 248
>At1g79930.1 68414.m09340 heat shock protein, putative contains Pfam
profile: PF00012 Heat shock hsp70 proteins; similar to
heat-shock proteins GB:CAA94389, GB:AAD55461
[Arabidopsis thaliana]
Length = 831
Score = 27.1 bits (57), Expect = 4.5
Identities = 14/52 (26%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Frame = +2
Query: 5 EKLKEKISDSDKQTILDKCNNTIKWL-DSNQLADKEEYEHKQKELEGIYNPI 157
+K +E I+DS+++ L WL + + K Y K +EL+ + +P+
Sbjct: 639 DKYQEYITDSEREAFLANLQEVEDWLYEDGEDETKGVYVAKLEELKKVGDPV 690
>At3g60000.1 68416.m06699 hypothetical protein contains Pfam
profile: PF04484 family of unknown function (DUF566)
Length = 451
Score = 26.2 bits (55), Expect = 7.9
Identities = 16/51 (31%), Positives = 23/51 (45%)
Frame = +3
Query: 105 RRSMSTSRKNWKAFTIR*LRRCTRVPEESPEVCRASRAEHPEPEVPPPGLR 257
R S + +R FT+ R+ ++ P + R E P P VPPP R
Sbjct: 88 RSSETAARSLDSPFTLSQSRKSSKPSHLKPLNENSHRLETPTPMVPPPPSR 138
>At3g19050.1 68416.m02420 kinesin motor protein-related contains Pfam
profile: PF00225 Kinesin motor domain; contains
non-consensus splice site (GC) at intron 12
Length = 2722
Score = 26.2 bits (55), Expect = 7.9
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +2
Query: 32 SDKQTILDKCNNTIKWLDSNQLADKEEYEHKQKELEGI 145
+D++T + + + I L+ + A EY HK KELE +
Sbjct: 2336 ADQKTEITQLSEHISELNLHAEAQASEYMHKFKELEAM 2373
>At1g44910.1 68414.m05146 FF domain-containing protein / WW
domain-containing protein contains Pfam profiles
PF01846: FF domain, PF00397: WW domain
Length = 946
Score = 26.2 bits (55), Expect = 7.9
Identities = 13/46 (28%), Positives = 27/46 (58%)
Frame = +2
Query: 8 KLKEKISDSDKQTILDKCNNTIKWLDSNQLADKEEYEHKQKELEGI 145
K+++++ D D+ + L+K + I + + +KEE E K+ E E +
Sbjct: 561 KIQDRLEDDDRCSCLEKIDRLIGFEEYILDLEKEEEELKRVEKEHV 606
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,469,414
Number of Sequences: 28952
Number of extensions: 141192
Number of successful extensions: 620
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 593
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 619
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 567552648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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