BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV30529X (354 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g66310.1 68418.m08360 kinesin motor family protein contains P... 27 2.7 At1g34260.1 68414.m04252 phosphatidylinositol-4-phosphate 5-kina... 27 4.7 At1g52855.1 68414.m05976 expressed protein 26 6.3 At1g77480.2 68414.m09023 nucellin protein, putative similar to n... 26 8.3 At1g77480.1 68414.m09022 nucellin protein, putative similar to n... 26 8.3 At1g07330.1 68414.m00781 hypothetical protein 26 8.3 >At5g66310.1 68418.m08360 kinesin motor family protein contains Pfam domain, PF00225: Kinesin motor domain Length = 1063 Score = 27.5 bits (58), Expect = 2.7 Identities = 15/43 (34%), Positives = 25/43 (58%) Frame = -3 Query: 130 RGRRTNMLKLRVFLTGLAXAWLRQAIATNANKTRDMTVTACAR 2 RG + + L L V L L ++R++ A N+N +MT+ +C R Sbjct: 916 RGDQKDCLYLEVELRRLK--YIRESFAQNSNDGNNMTLISCTR 956 >At1g34260.1 68414.m04252 phosphatidylinositol-4-phosphate 5-kinase family protein low similarity to SP|Q9Z1T6 FYVE finger-containing phosphoinositide kinase (EC 2.7.1.68) (1- phosphatidylinositol-4-phosphate kinase) (PIP5K) (PtdIns(4)P-5-kinase) {Mus musculus}; contains Pfam profile PF01504: Phosphatidylinositol-4-phosphate 5-Kinase Length = 1456 Score = 26.6 bits (56), Expect = 4.7 Identities = 16/49 (32%), Positives = 24/49 (48%) Frame = +2 Query: 197 GATRTRNDVG*EASGED*GNHSELIENVSTSIPNAAENEVDGVPESKMP 343 G TR ND SG + G + L+E+ I A+ + D + ES +P Sbjct: 920 GMTRYENDNKVSDSGSNGGIDTPLVEHKDIPIAGASVGDNDQMAESYVP 968 >At1g52855.1 68414.m05976 expressed protein Length = 68 Score = 26.2 bits (55), Expect = 6.3 Identities = 11/35 (31%), Positives = 14/35 (40%) Frame = +1 Query: 94 KPSISTCLSCGHDRTPKIFTRHGRVSRLGRYWNIW 198 KP+ TC CGH + R + YW W Sbjct: 18 KPAAGTCSRCGHCASIADMKTSTRFCFIPIYWRSW 52 >At1g77480.2 68414.m09023 nucellin protein, putative similar to nucellin GB:AAB96882 GI:2290202 [Hordeum vulgare] (nucellin: similar to aspartic protease and its specific expression in nucellar cells during degeneration) Length = 432 Score = 25.8 bits (54), Expect = 8.3 Identities = 17/47 (36%), Positives = 21/47 (44%) Frame = -2 Query: 188 QYRPNLDTRPWRVKIFGVLSWPQDKHVEIEGFLDGPGXCLVEAGYSD 48 QY+PN +T P + L PQD+ D C E GYSD Sbjct: 109 QYKPNHNTLPCSHILCSGLDLPQDRPC-----ADPEDQCDYEIGYSD 150 >At1g77480.1 68414.m09022 nucellin protein, putative similar to nucellin GB:AAB96882 GI:2290202 [Hordeum vulgare] (nucellin: similar to aspartic protease and its specific expression in nucellar cells during degeneration) Length = 466 Score = 25.8 bits (54), Expect = 8.3 Identities = 17/47 (36%), Positives = 21/47 (44%) Frame = -2 Query: 188 QYRPNLDTRPWRVKIFGVLSWPQDKHVEIEGFLDGPGXCLVEAGYSD 48 QY+PN +T P + L PQD+ D C E GYSD Sbjct: 109 QYKPNHNTLPCSHILCSGLDLPQDRPC-----ADPEDQCDYEIGYSD 150 >At1g07330.1 68414.m00781 hypothetical protein Length = 685 Score = 25.8 bits (54), Expect = 8.3 Identities = 14/58 (24%), Positives = 29/58 (50%) Frame = -1 Query: 297 FGIDVDTFSINSE*FPQSSPEAS*PTSFLVRVAPNIPIPPQSGYPSVASKNLRRPVVA 124 FG+D + + ++ P+S+P PT + +IP PQ P+++ + ++ A Sbjct: 219 FGLDQENYIVDGLQMPESAPSVLLPT----KNPFDIPYDPQEEKPNLSGDSFQQEFAA 272 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 8,320,080 Number of Sequences: 28952 Number of extensions: 170240 Number of successful extensions: 419 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 396 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 419 length of database: 12,070,560 effective HSP length: 72 effective length of database: 9,986,016 effective search space used: 449370720 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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