Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30494
(741 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g20060.2 68418.m02389 phospholipase/carboxylesterase family p... 67 1e-11
At5g20060.1 68418.m02388 phospholipase/carboxylesterase family p... 67 1e-11
At3g15650.1 68416.m01984 phospholipase/carboxylesterase family p... 63 2e-10
At1g52700.1 68414.m05952 phospholipase/carboxylesterase family p... 63 2e-10
At4g22300.1 68417.m03225 phospholipase/carboxylesterase family p... 50 2e-06
At1g47786.1 68414.m05316 acyl-protein thioesterase-related simil... 46 3e-05
At1g52695.1 68414.m05951 phospholipase/carboxylesterase family p... 45 5e-05
At1g47780.1 68414.m05314 acyl-protein thioesterase-related conta... 38 0.005
At1g51300.1 68414.m05769 acyl-protein thioesterase-related cont... 38 0.009
At5g35604.1 68418.m04242 hypothetical protein 32 0.46
At3g50590.1 68416.m05533 transducin family protein / WD-40 repea... 29 4.3
At3g12460.1 68416.m01551 hypothetical protein 29 4.3
At2g40070.1 68415.m04923 expressed protein 28 7.5
At2g23580.1 68415.m02814 hydrolase, alpha/beta fold family prote... 28 7.5
At3g60040.1 68416.m06705 F-box family protein contains a novel d... 27 9.9
At3g52240.1 68416.m05741 expressed protein 27 9.9
At2g45680.1 68415.m05680 TCP family transcription factor, putati... 27 9.9
At2g36470.1 68415.m04476 expressed protein 27 9.9
>At5g20060.2 68418.m02389 phospholipase/carboxylesterase family
protein similar to lysophospholipase II [Mus musculus]
GI:4589453; contains Pfam profile PF02230:
Phospholipase/Carboxylesterase family
Length = 252
Score = 67.3 bits (157), Expect = 1e-11
Identities = 24/63 (38%), Positives = 42/63 (66%)
Frame = +3
Query: 48 IIAAQARHTASLIFLHGLGDTGHGWASTIAGIRGPHVKVICPTASTMPVTLNNGFRMPSW 227
++ + +H A++++LHGLGD G W+ + + P++K ICPTA + P++L GF +W
Sbjct: 25 VVRPKGKHQATIVWLHGLGDNGSSWSQLLETLPLPNIKWICPTAPSQPISLFGGFPSTAW 84
Query: 228 FDL 236
FD+
Sbjct: 85 FDV 87
Score = 36.3 bits (80), Expect = 0.021
Identities = 19/56 (33%), Positives = 29/56 (51%), Gaps = 1/56 (1%)
Frame = +1
Query: 511 HGDKDPVVSFKWGQMTA-SCLKTFMKNVKFSTYQGLAHSSSIAELKDMQEFIEKTL 675
HG D VV FK+G+ ++ + L K V F Y L H + EL ++ ++ TL
Sbjct: 193 HGKADDVVPFKFGEKSSQALLSNGFKKVTFKPYSALGHHTIPQELDELCAWLTSTL 248
>At5g20060.1 68418.m02388 phospholipase/carboxylesterase family
protein similar to lysophospholipase II [Mus musculus]
GI:4589453; contains Pfam profile PF02230:
Phospholipase/Carboxylesterase family
Length = 252
Score = 67.3 bits (157), Expect = 1e-11
Identities = 24/63 (38%), Positives = 42/63 (66%)
Frame = +3
Query: 48 IIAAQARHTASLIFLHGLGDTGHGWASTIAGIRGPHVKVICPTASTMPVTLNNGFRMPSW 227
++ + +H A++++LHGLGD G W+ + + P++K ICPTA + P++L GF +W
Sbjct: 25 VVRPKGKHQATIVWLHGLGDNGSSWSQLLETLPLPNIKWICPTAPSQPISLFGGFPSTAW 84
Query: 228 FDL 236
FD+
Sbjct: 85 FDV 87
Score = 36.3 bits (80), Expect = 0.021
Identities = 19/56 (33%), Positives = 29/56 (51%), Gaps = 1/56 (1%)
Frame = +1
Query: 511 HGDKDPVVSFKWGQMTA-SCLKTFMKNVKFSTYQGLAHSSSIAELKDMQEFIEKTL 675
HG D VV FK+G+ ++ + L K V F Y L H + EL ++ ++ TL
Sbjct: 193 HGKADDVVPFKFGEKSSQALLSNGFKKVTFKPYSALGHHTIPQELDELCAWLTSTL 248
>At3g15650.1 68416.m01984 phospholipase/carboxylesterase family
protein low similarity to lysophospholipase I [Mus
musculus] GI:1864159; contains Pfam profile PF02230:
Phospholipase/Carboxylesterase family
Length = 255
Score = 62.9 bits (146), Expect = 2e-10
Identities = 25/70 (35%), Positives = 43/70 (61%)
Frame = +3
Query: 48 IIAAQARHTASLIFLHGLGDTGHGWASTIAGIRGPHVKVICPTASTMPVTLNNGFRMPSW 227
++ + +H A++++LHGLGD G + + + P++K ICPTA + PV+L GF +W
Sbjct: 25 VVRPKGKHQATIVWLHGLGDNGSSSSQLLESLPLPNIKWICPTAPSRPVSLLGGFPCTAW 84
Query: 228 FDLRTLDATL 257
FD+ + L
Sbjct: 85 FDVGEISEDL 94
>At1g52700.1 68414.m05952 phospholipase/carboxylesterase family
protein similar to lysophospholipase I [Mus musculus]
GI:1864159; contains Pfam profile PF02230:
Phospholipase/Carboxylesterase family
Length = 255
Score = 62.9 bits (146), Expect = 2e-10
Identities = 25/63 (39%), Positives = 40/63 (63%)
Frame = +3
Query: 48 IIAAQARHTASLIFLHGLGDTGHGWASTIAGIRGPHVKVICPTASTMPVTLNNGFRMPSW 227
++ + +H A+L++LHGLGD G + + + P++K ICPTA + PVT GF +W
Sbjct: 25 VVRPKGKHQATLVWLHGLGDNGSSSSQLMDSLHLPNIKWICPTAPSRPVTSLGGFTCTAW 84
Query: 228 FDL 236
FD+
Sbjct: 85 FDV 87
>At4g22300.1 68417.m03225 phospholipase/carboxylesterase family
protein similar to acyl-protein thioesterase-1 [Homo
sapiens] GI:9965372; contains Pfam profile PF02230:
Phospholipase/Carboxylesterase family
Length = 471
Score = 50.0 bits (114), Expect = 2e-06
Identities = 22/66 (33%), Positives = 34/66 (51%)
Frame = +2
Query: 254 TPEDEEGIERATDLVHGLIADEVKAGVPADKVLLGGFSQGGXXXXXXXXTYPERLAGVMS 433
+P+DE + +A VH +I E+ G+ + V + GFSQGG YP+ + G
Sbjct: 354 SPKDESSLLKAVKNVHAIIDKEIAGGINPENVYICGFSQGGALTLASVLLYPKTIGGGAV 413
Query: 434 LSCWLP 451
S W+P
Sbjct: 414 FSGWIP 419
Score = 46.8 bits (106), Expect = 2e-05
Identities = 23/66 (34%), Positives = 31/66 (46%)
Frame = +2
Query: 254 TPEDEEGIERATDLVHGLIADEVKAGVPADKVLLGGFSQGGXXXXXXXXTYPERLAGVMS 433
+P DE + A VH +I E+ G + V + G SQGG YP+ L G
Sbjct: 69 SPIDESSVLEAVKNVHAIIDQEIAEGTNPENVFICGLSQGGALTLASVLLYPKTLGGGAV 128
Query: 434 LSCWLP 451
LS W+P
Sbjct: 129 LSGWVP 134
Score = 40.7 bits (91), Expect = 0.001
Identities = 23/61 (37%), Positives = 32/61 (52%), Gaps = 3/61 (4%)
Frame = +3
Query: 81 LIFLHGLGDTGHGWASTIAGIRGP---HVKVICPTASTMPVTLNNGFRMPSWFDLRTLDA 251
+++LHGLGD+G R + K + P+A PV+ N G MPSWFD+ L
Sbjct: 291 ILWLHGLGDSGPANEPIKTLFRSQEFRNTKWLFPSAPPNPVSCNYGAVMPSWFDIPELPL 350
Query: 252 T 254
T
Sbjct: 351 T 351
Score = 39.9 bits (89), Expect = 0.002
Identities = 24/66 (36%), Positives = 33/66 (50%), Gaps = 3/66 (4%)
Frame = +1
Query: 487 C*FTNIP---THGDKDPVVSFKWGQMTASCLKTFMKNVKFSTYQGLAHSSSIAELKDMQE 657
C N P +HG D +V F+ GQ LK +F Y GL HS S ELK ++
Sbjct: 154 CFLINTPILWSHGTDDRMVLFEAGQAALPFLKEAGVTCEFKAYPGLGHSISNKELKYIES 213
Query: 658 FIEKTL 675
+I++ L
Sbjct: 214 WIKRRL 219
Score = 37.5 bits (83), Expect = 0.009
Identities = 21/58 (36%), Positives = 30/58 (51%), Gaps = 3/58 (5%)
Frame = +3
Query: 81 LIFLHGLGDTGHGWASTIAGIRGPHVKV---ICPTASTMPVTLNNGFRMPSWFDLRTL 245
+++LHGLGD+G + + + P+A PVT NNG M SWFD+ L
Sbjct: 6 ILWLHGLGDSGPANEPIQTQFKSSELSNASWLFPSAPFNPVTCNNGAVMRSWFDVPEL 63
>At1g47786.1 68414.m05316 acyl-protein thioesterase-related similar
to hypothetical protein GB:AAD55623 GI:5903064 from
[Arabidopsis thaliana] contains similarity to
acyl-protein thioesterase-1 [Homo sapiens]
gi|9965372|gb|AAG10063
Length = 186
Score = 45.6 bits (103), Expect = 3e-05
Identities = 19/63 (30%), Positives = 33/63 (52%)
Frame = +3
Query: 69 HTASLIFLHGLGDTGHGWASTIAGIRGPHVKVICPTASTMPVTLNNGFRMPSWFDLRTLD 248
H A++++LH +G+T + ++K ICPTA PVT+ G +WFD+ +
Sbjct: 55 HKATIVWLHDIGETSANSVRFARQLGLRNIKWICPTAPRRPVTILGGMETNAWFDIAEIS 114
Query: 249 ATL 257
+
Sbjct: 115 ENM 117
>At1g52695.1 68414.m05951 phospholipase/carboxylesterase family
protein contains Pfam profile: PF02230
phospholipase/carboxylesterase; supported by full length
cDNA gi:26450919 from [Arabidopsis thaliana]
Length = 231
Score = 45.2 bits (102), Expect = 5e-05
Identities = 20/56 (35%), Positives = 32/56 (57%)
Frame = +3
Query: 69 HTASLIFLHGLGDTGHGWASTIAGIRGPHVKVICPTASTMPVTLNNGFRMPSWFDL 236
H A++++LH +G+TG + +R P++K ICPTA VT G +W D+
Sbjct: 25 HKATIVWLHDVGNTGFNSLEPLQNLRLPNIKWICPTAPRRRVTSLGGEITNAWCDI 80
>At1g47780.1 68414.m05314 acyl-protein thioesterase-related contains
similarity to acyl-protein thioesterase-1 [Homo sapiens]
gi|9965372|gb|AAG10063 contains similarity to
acyl-protein thioesterase-1 [Homo sapiens]
gi|9965372|gb|AAG10063
Length = 126
Score = 38.3 bits (85), Expect = 0.005
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = +3
Query: 120 WASTIAGIRGPHVKVICPTASTMPVTLNNGFRMPSWFDLRTLDATL 257
W + G +VK ICPTA P+T+ G +WFD+ L +
Sbjct: 12 WVLKMYGWMNKNVKWICPTAPRRPLTILGGMETNAWFDIAELSENM 57
>At1g51300.1 68414.m05769 acyl-protein thioesterase-related
contains similarity to acyl-protein thioesterase-1 [Homo
sapiens] gi|9965372|gb|AAG10063
Length = 212
Score = 37.5 bits (83), Expect = 0.009
Identities = 18/56 (32%), Positives = 33/56 (58%)
Frame = +3
Query: 45 VIIAAQARHTASLIFLHGLGDTGHGWASTIAGIRGPHVKVICPTASTMPVTLNNGF 212
V + +ARH A++++LH L ++G+ + + +VK ICP++ P+ N GF
Sbjct: 28 VTVTPRARHQATIVWLHDLNESGYDSSELVKSFSLYNVKWICPSS---PLISNVGF 80
>At5g35604.1 68418.m04242 hypothetical protein
Length = 298
Score = 31.9 bits (69), Expect = 0.46
Identities = 25/85 (29%), Positives = 37/85 (43%), Gaps = 3/85 (3%)
Frame = -1
Query: 540 ERYNWVLITMSWNI--GKSTGAFKPPGK*PCRGSQQDRDMTPANLSGYVSAAY-RANAPP 370
E Y W+L+ + +S AF+P RG + R + N S A R + P
Sbjct: 35 EDYTWILLVDDGSAEGARSAYAFRPTLHREHRGGRSPRRPSRGNSSPRRDKARARTDCSP 94
Query: 369 *EKPPSRTLSAGTPALTSSAINPWT 295
PPSRT+ P TS + + W+
Sbjct: 95 RLSPPSRTMGPPPPVATSPS-SQWS 118
>At3g50590.1 68416.m05533 transducin family protein / WD-40 repeat
family protein contains 3 WD-40 repeats (PF00400); some
similarity to s-tomosyn isoform (GI:4689231)[Rattus
norvegicus]; contains non-consensus AT-AC splice sites
at intron 18
Length = 1606
Score = 28.7 bits (61), Expect = 4.3
Identities = 16/45 (35%), Positives = 23/45 (51%), Gaps = 2/45 (4%)
Frame = -3
Query: 151 GPLMPAIVLAHP*PVSPKPCKNISDAVCLA--CAAIITGFGSILQ 23
G L ++LAHP +SPK K I CL ++ GF ++ Q
Sbjct: 769 GALNDRLLLAHPTDISPKQKKGIEIKSCLVGLLEPLLIGFSTMQQ 813
>At3g12460.1 68416.m01551 hypothetical protein
Length = 242
Score = 28.7 bits (61), Expect = 4.3
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = +1
Query: 619 HSSSIAELKDMQEFIEKTLPASK*MYHLLLEKII 720
H IAEL D++EF+ + + MY+ LEKI+
Sbjct: 155 HQLEIAELMDLREFVSDS-SGRRSMYNYSLEKIV 187
>At2g40070.1 68415.m04923 expressed protein
Length = 607
Score = 27.9 bits (59), Expect = 7.5
Identities = 27/90 (30%), Positives = 44/90 (48%), Gaps = 4/90 (4%)
Frame = -1
Query: 516 TMSWNIGKSTGAFKPPGK*PCRGSQQDRDMTPANLSGYVSAAYRANAPP*EKPPSR-TLS 340
T S + S+GA + P G R TP S ++A +++ P P SR T+S
Sbjct: 161 TSSPGLSSSSGASRRPSS---SGGPGSRPATPTGRSSTLTANSKSSRP--STPTSRATVS 215
Query: 339 AGT-PALTS--SAINPWTRSVALSIPSSSS 259
+ T P+LT+ S ++ T+ +S +S S
Sbjct: 216 SATRPSLTNSRSTVSATTKPTPMSRSTSLS 245
>At2g23580.1 68415.m02814 hydrolase, alpha/beta fold family protein
similar to ethylene-induced esterase [Citrus sinensis]
GI:14279437, polyneuridine aldehyde esterase [Rauvolfia
serpentina] GI:6651393; contains Pfam profile PF00561:
hydrolase, alpha/beta fold family
Length = 263
Score = 27.9 bits (59), Expect = 7.5
Identities = 16/61 (26%), Positives = 27/61 (44%)
Frame = +2
Query: 269 EGIERATDLVHGLIADEVKAGVPADKVLLGGFSQGGXXXXXXXXTYPERLAGVMSLSCWL 448
E I+ D L+ G DKV+L S GG +P ++A ++ L+ ++
Sbjct: 52 EEIQTLKDYCKPLLELLNSLGSDDDKVILVAHSMGGIPAALASDIFPSKIATIVFLTAFM 111
Query: 449 P 451
P
Sbjct: 112 P 112
>At3g60040.1 68416.m06705 F-box family protein contains a novel
domain with similarity to F-box domain;
Length = 838
Score = 27.5 bits (58), Expect = 9.9
Identities = 14/38 (36%), Positives = 23/38 (60%)
Frame = -2
Query: 692 IYFDAGRVFSINSCMSLSSAMDELCASPWYVENLTFFI 579
IY D + SIN+ L+ ++DEL + + V LTF++
Sbjct: 146 IYNDVNNISSINAFQDLAGSLDELKTTSFIVV-LTFWV 182
>At3g52240.1 68416.m05741 expressed protein
Length = 680
Score = 27.5 bits (58), Expect = 9.9
Identities = 20/64 (31%), Positives = 32/64 (50%), Gaps = 1/64 (1%)
Frame = -1
Query: 447 SQQDRDMTPANLSG-YVSAAYRANAPP*EKPPSRTLSAGTPALTSSAINPWTRSVALSIP 271
S DR T + G + +++RA+ PP P L+ G P ++ SAI+ T +
Sbjct: 569 SVDDRLSTTPYIGGPKLWSSFRADGPPMVDVPGFRLNKGIPRVSGSAISGGTSGFEGNQV 628
Query: 270 SSSS 259
SSS+
Sbjct: 629 SSST 632
>At2g45680.1 68415.m05680 TCP family transcription factor, putative
similar to PCF2 (GI:2580440) [Oryza sativa]
Length = 356
Score = 27.5 bits (58), Expect = 9.9
Identities = 17/56 (30%), Positives = 31/56 (55%)
Frame = -1
Query: 420 ANLSGYVSAAYRANAPP*EKPPSRTLSAGTPALTSSAINPWTRSVALSIPSSSSGV 253
A+ S YV+A +A+ P S+G +++ + + +R+ ++ PSSSSGV
Sbjct: 258 ASPSSYVAAVQQASTMARPPPLQVVPSSGFVSVSDVSGSNLSRATSVMAPSSSSGV 313
>At2g36470.1 68415.m04476 expressed protein
Length = 327
Score = 27.5 bits (58), Expect = 9.9
Identities = 16/54 (29%), Positives = 26/54 (48%)
Frame = -1
Query: 423 PANLSGYVSAAYRANAPP*EKPPSRTLSAGTPALTSSAINPWTRSVALSIPSSS 262
P ++ V+ YR+N P +KP S +L+ + P + SV+L P S
Sbjct: 34 PPSIPDTVTTTYRSNLPSSDKPVSVSLTWSDNLTVVISTPPKSYSVSLRKPKGS 87
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,012,223
Number of Sequences: 28952
Number of extensions: 380268
Number of successful extensions: 1000
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 950
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 995
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1633819784
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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