Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30481
(746 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g27670.1 68417.m03979 25.3 kDa small heat shock protein, chlo... 45 5e-05
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 45 6e-05
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 44 1e-04
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 42 3e-04
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 42 6e-04
At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein (HS... 41 0.001
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 40 0.002
At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock pro... 34 0.087
At5g55300.1 68418.m06891 DNA topoisomerase I identical to Swiss-... 29 4.3
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 29 4.3
At4g34020.1 68417.m04827 DJ-1 family protein similar to CAP1 [Ra... 29 4.3
At3g53500.2 68416.m05907 zinc knuckle (CCHC-type) family protein... 28 5.7
At3g53500.1 68416.m05906 zinc knuckle (CCHC-type) family protein... 28 5.7
At5g59520.1 68418.m07459 zinc transporter (ZIP2) identical to zi... 27 10.0
At2g38410.1 68415.m04718 VHS domain-containing protein / GAT dom... 27 10.0
At1g53330.1 68414.m06045 pentatricopeptide (PPR) repeat-containi... 27 10.0
At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase... 27 10.0
>At4g27670.1 68417.m03979 25.3 kDa small heat shock protein,
chloroplast precursor (HSP25.3-P) identical to small
heat shock protein, chloroplast precursor SP:P31170 from
[Arabidopsis thaliana]; identified in Scharf, K-D., et
al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 227
Score = 45.2 bits (102), Expect = 5e-05
Identities = 19/89 (21%), Positives = 46/89 (51%), Gaps = 3/89 (3%)
Frame = +1
Query: 268 KFQVNLDVQHFSPEEISVKTADGYVIVEGKHEERQDEHGYISRQ---FTRRYALPENCNP 438
+ ++ D+ S E++ + D ++++G+ ++ + + R + R LP+NC
Sbjct: 137 EIKMRFDMPGLSKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVSSYGTRLQLPDNCEK 196
Query: 439 DTVESRLSSDGVLTVIAPRTPAARRTSEL 525
D +++ L +GVL + P+T R+ ++
Sbjct: 197 DKIKAEL-KNGVLFITIPKTKVERKVIDV 224
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 44.8 bits (101), Expect = 6e-05
Identities = 28/88 (31%), Positives = 49/88 (55%), Gaps = 8/88 (9%)
Frame = +1
Query: 286 DVQHFSPEEISVKTADGYVI-VEGKH----EERQDEHGYISR---QFTRRYALPENCNPD 441
D+ EE+ V+ D V+ + G+ EE+QD + R QF+R++ LPEN D
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123
Query: 442 TVESRLSSDGVLTVIAPRTPAARRTSEL 525
V++ + +GVLTV P+ A++ +++
Sbjct: 124 QVKASM-ENGVLTVTVPKVEEAKKKAQV 150
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 43.6 bits (98), Expect = 1e-04
Identities = 25/80 (31%), Positives = 46/80 (57%), Gaps = 8/80 (10%)
Frame = +1
Query: 286 DVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPD 441
D+ EE+ V+ DG ++ + G+ +EE+ D+ + R +FTRR+ LPEN +
Sbjct: 66 DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125
Query: 442 TVESRLSSDGVLTVIAPRTP 501
+++ + +GVL+V P+ P
Sbjct: 126 EIKASM-ENGVLSVTVPKVP 144
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 42.3 bits (95), Expect = 3e-04
Identities = 26/80 (32%), Positives = 45/80 (56%), Gaps = 8/80 (10%)
Frame = +1
Query: 286 DVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPD 441
D+ EE+ V+ D V+ + G+ +EE+ D+ + R +F RR+ LPEN +
Sbjct: 68 DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127
Query: 442 TVESRLSSDGVLTVIAPRTP 501
V++ + +GVLTV+ P+ P
Sbjct: 128 EVKATM-ENGVLTVVVPKAP 146
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 41.5 bits (93), Expect = 6e-04
Identities = 27/78 (34%), Positives = 44/78 (56%), Gaps = 8/78 (10%)
Frame = +1
Query: 286 DVQHFSPEEISVKTADGYVI-VEG-KHEERQDEHGYISR------QFTRRYALPENCNPD 441
D+ EE+ V+ + V+ + G +H E++D++ R QFTRR+ LPEN D
Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121
Query: 442 TVESRLSSDGVLTVIAPR 495
V++ + +GVLTV P+
Sbjct: 122 QVKAAM-ENGVLTVTVPK 138
>At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein
(HSP17.6A-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]
Length = 155
Score = 40.7 bits (91), Expect = 0.001
Identities = 26/88 (29%), Positives = 47/88 (53%), Gaps = 8/88 (9%)
Frame = +1
Query: 286 DVQHFSPEEISVKTADGYVI-VEGKH----EERQDEHGYISRQ---FTRRYALPENCNPD 441
D+ EE+ V+ D V+ + G+ EE+QD + R F+R++ LPEN D
Sbjct: 62 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGGFSRKFRLPENVKMD 121
Query: 442 TVESRLSSDGVLTVIAPRTPAARRTSEL 525
V++ + +GVLTV P+ ++ +++
Sbjct: 122 QVKASM-ENGVLTVTVPKVETNKKKAQV 148
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 39.9 bits (89), Expect = 0.002
Identities = 25/83 (30%), Positives = 45/83 (54%), Gaps = 8/83 (9%)
Frame = +1
Query: 286 DVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPD 441
DV EE+ V+ DG ++ + G+ +EE+ D + R +F RR+ LPEN +
Sbjct: 65 DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVE 124
Query: 442 TVESRLSSDGVLTVIAPRTPAAR 510
V++ + +GVL+V P+ ++
Sbjct: 125 EVKASM-ENGVLSVTVPKVQESK 146
>At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock
protein (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family: identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 134
Score = 34.3 bits (75), Expect = 0.087
Identities = 22/83 (26%), Positives = 36/83 (43%)
Frame = +1
Query: 265 DKFQVNLDVQHFSPEEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDT 444
D ++D+ EEI V+ D ++ + F R++ LPE+ +
Sbjct: 35 DSHTFSVDLPGLRKEEIKVEIEDSIYLIIRTEATPMSPPDQPLKTFKRKFRLPESIDMIG 94
Query: 445 VESRLSSDGVLTVIAPRTPAARR 513
+ + DGVLTVI P+ RR
Sbjct: 95 ISAGYE-DGVLTVIVPKRIMTRR 116
>At5g55300.1 68418.m06891 DNA topoisomerase I identical to
Swiss-Prot:P30181 DNA topoisomerase I [Arabidopsis
thaliana]
Length = 916
Score = 28.7 bits (61), Expect = 4.3
Identities = 11/37 (29%), Positives = 18/37 (48%)
Frame = +3
Query: 489 SQDSGCQKNERAVPITQTGPVRKEIKEPTAEVESNET 599
+Q + +AVP T+ P+R + P SN+T
Sbjct: 37 NQSKSNSQRSKAVPTTKVSPMRSPVTSPNGTTPSNKT 73
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 28.7 bits (61), Expect = 4.3
Identities = 16/54 (29%), Positives = 22/54 (40%)
Frame = +1
Query: 307 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSD 468
+EI D Y EG EE ++ R + L E+C + VES D
Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDD 101
>At4g34020.1 68417.m04827 DJ-1 family protein similar to CAP1
[Rattus norvegicus] GI:3250916, ThiJ protein,
Escherichia coli, PIR:H64771; contains Pfam profile
PF01965 ThiJ/PfpI family
Length = 472
Score = 28.7 bits (61), Expect = 4.3
Identities = 16/43 (37%), Positives = 20/43 (46%)
Frame = -3
Query: 456 PRFDSVRVAVFRQSVTPRELTRDVAMLVLPLLVLTFDDDVAVG 328
P F SV V T R+ T + + P +V T D DV VG
Sbjct: 36 PSFSSVSVVSSSLGTTRRDRTLKLRSSMSPGMVTTLDSDVGVG 78
>At3g53500.2 68416.m05907 zinc knuckle (CCHC-type) family protein
contains Pfam domain PF00098: Zinc knuckle
Length = 284
Score = 28.3 bits (60), Expect = 5.7
Identities = 20/77 (25%), Positives = 38/77 (49%), Gaps = 2/77 (2%)
Frame = +1
Query: 418 LPENC--NPDTVESRLSSDGVLTVIAPRTPAARRTSELFPSLKPVRSGRRLRSPLRKLRA 591
+ NC +P ++R + + R+P RR+ S RS R RSP+R+ ++
Sbjct: 132 IERNCKNSPSPKKARQGGSYSRSPVKSRSPRRRRSPSRSRSYSRGRSYSRSRSPVRREKS 191
Query: 592 TKQNNDSRNAVNQKSVA 642
+ + S A+ ++SV+
Sbjct: 192 VEDRSRSPKAM-ERSVS 207
>At3g53500.1 68416.m05906 zinc knuckle (CCHC-type) family protein
contains Pfam domain PF00098: Zinc knuckle
Length = 243
Score = 28.3 bits (60), Expect = 5.7
Identities = 20/77 (25%), Positives = 38/77 (49%), Gaps = 2/77 (2%)
Frame = +1
Query: 418 LPENC--NPDTVESRLSSDGVLTVIAPRTPAARRTSELFPSLKPVRSGRRLRSPLRKLRA 591
+ NC +P ++R + + R+P RR+ S RS R RSP+R+ ++
Sbjct: 91 IERNCKNSPSPKKARQGGSYSRSPVKSRSPRRRRSPSRSRSYSRGRSYSRSRSPVRREKS 150
Query: 592 TKQNNDSRNAVNQKSVA 642
+ + S A+ ++SV+
Sbjct: 151 VEDRSRSPKAM-ERSVS 166
>At5g59520.1 68418.m07459 zinc transporter (ZIP2) identical to zinc
transporter ZIP2 [Arabidopsis thaliana]
gi|3252868|gb|AAC24198; member of the Zinc (Zn2+)-Iron
(Fe2+) permease (ZIP) family, PMID:11500563
Length = 353
Score = 27.5 bits (58), Expect = 10.0
Identities = 20/48 (41%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Frame = -3
Query: 348 DDDVAVGCLH*DFFGRKVLNV*VDLEFVLIRVM-VEPTSLLAIAICFH 208
DDDVAV GR+ + VD+ LIR T+LL A+CFH
Sbjct: 170 DDDVAV-----KEEGRREIKSGVDVSQALIRTSGFGDTALLIFALCFH 212
>At2g38410.1 68415.m04718 VHS domain-containing protein / GAT
domain-containing protein weak similarity to hepatocyte
growth factor-regulated tyrosine kinase substrate HRS
isoform 2 [Homo sapiens] GI:9022389; contains Pfam
profiles PF00790: VHS domain, PF03127: GAT domain
Length = 671
Score = 27.5 bits (58), Expect = 10.0
Identities = 16/74 (21%), Positives = 35/74 (47%)
Frame = +1
Query: 301 SPEEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLT 480
S I + G ++ ++EE +DE ++R+ ++ A +P ++ES ++ L
Sbjct: 356 SSSPIPATVSTGKSPIDEEYEEEEDEFAQLARRHSKPPA-SVTTDPTSLESHNAASNALA 414
Query: 481 VIAPRTPAARRTSE 522
+ P P T++
Sbjct: 415 LALPDPPPPVNTTK 428
>At1g53330.1 68414.m06045 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 471
Score = 27.5 bits (58), Expect = 10.0
Identities = 22/74 (29%), Positives = 32/74 (43%)
Frame = +1
Query: 364 ERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTVIAPRTPAARRTSELFPSLKP 543
++ +E Y+ RR C+PDT+ R+ DG+ + A LF KP
Sbjct: 342 KKWEEATYLFEDMPRR-----GCSPDTLSYRIVFDGLCEGLQFEEAAVILDEMLFKGYKP 396
Query: 544 VRSGRRLRSPLRKL 585
R RL L+KL
Sbjct: 397 RRD--RLEGFLQKL 408
>At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase
large subunit 2 (APL2) / ADP-glucose pyrophosphorylase
identical to SP|P55230
Length = 518
Score = 27.5 bits (58), Expect = 10.0
Identities = 15/59 (25%), Positives = 26/59 (44%)
Frame = +1
Query: 307 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTV 483
E+ K + +I+ G H R D ++ + + +C P ESR S G+L +
Sbjct: 198 EDAKTKNVEHVLILSGDHLYRMDYMNFVQKHIESNADITVSCLP-MDESRASDFGLLKI 255
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,717,194
Number of Sequences: 28952
Number of extensions: 299391
Number of successful extensions: 966
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 937
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 965
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1653386488
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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