BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV30475 (716 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g41530.1 68415.m05132 esterase, putative similar to SP|P10768... 111 6e-25 At5g23575.1 68418.m02766 transmembrane protein, putative similar... 29 3.1 At3g42610.1 68416.m04423 hypothetical protein 28 7.1 At1g48390.1 68414.m05405 syntaxin-related family protein contain... 28 7.1 At3g20740.1 68416.m02624 fertilization-independent endosperm pro... 27 9.4 >At2g41530.1 68415.m05132 esterase, putative similar to SP|P10768 Esterase D (EC 3.1.1.1) {Homo sapiens}; contains Pfam profile: PF00756 putative esterase Length = 284 Score = 111 bits (266), Expect = 6e-25 Identities = 48/81 (59%), Positives = 64/81 (79%) Frame = +1 Query: 4 KLPLLYYLSGLTCSEQNFITKSGFQRYAAEHGVIVVGPDTSPRGVKIDGDDSSWDFGVSA 183 K P+LY+LSGLTC+++NFI KSG QR A+ HG+ +V PDTSPRG+ ++G+ S+DFGV A Sbjct: 47 KSPVLYWLSGLTCTDENFIIKSGAQRAASTHGIALVAPDTSPRGLNVEGEADSYDFGVGA 106 Query: 184 GFYLDATNEPWNNNYRMGSYL 246 GFYL+AT E W N+RM Y+ Sbjct: 107 GFYLNATQEKW-KNWRMYDYV 126 Score = 81.4 bits (192), Expect = 5e-16 Identities = 34/69 (49%), Positives = 48/69 (69%) Frame = +2 Query: 506 EYNGPPLTLLLDQGSGDKFYLEKQLLPENLVEACRSVGVPVILQLRDGYDHSYYYISTYI 685 +YN T+L+DQG D+FY + QLLP EAC+ V P++L+L GYDHSYY+I+T+I Sbjct: 213 KYNNLSATILIDQGENDQFYPD-QLLPSKFEEACKKVNAPLLLRLHPGYDHSYYFIATFI 271 Query: 686 GEHFDFHAK 712 +H HA+ Sbjct: 272 EDHISHHAQ 280 Score = 73.7 bits (173), Expect = 1e-13 Identities = 34/60 (56%), Positives = 42/60 (70%) Frame = +3 Query: 342 ALVSTLRNPGQYKSVSAFAPICNPSACPWGVKAFSGYLGEDKSKWAEWDATELVKNTMDL 521 AL LRN +YKSVSAFAPI NP C WG KAF+ YLG++K+ W E+DAT L+ +L Sbjct: 158 ALTIYLRNLDKYKSVSAFAPITNPINCAWGQKAFTNYLGDNKAAWEEYDATCLISKYNNL 217 >At5g23575.1 68418.m02766 transmembrane protein, putative similar to cleft lip and palate transmembrane protein 1 [Homo sapiens] GI:4039014; contains Pfam profile PF05602: Cleft lip and palate transmembrane protein 1 (CLPTM1) Length = 593 Score = 29.1 bits (62), Expect = 3.1 Identities = 13/40 (32%), Positives = 19/40 (47%) Frame = -1 Query: 176 TPKSHDESSPSILTPRGEVSGPTTITPCSAAYLWKPDLVM 57 +PK DES P + + S P P LWKP++ + Sbjct: 182 SPKDSDESEPEVEKVGDKKSDPKEEVPVEWISLWKPNVTI 221 >At3g42610.1 68416.m04423 hypothetical protein Length = 202 Score = 27.9 bits (59), Expect = 7.1 Identities = 18/64 (28%), Positives = 27/64 (42%), Gaps = 3/64 (4%) Frame = +2 Query: 518 PPLTLLLDQGSGDKFYLEKQLLPENLVEACRSVGVPVILQLRDGYD---HSYYYISTYIG 688 P TLLL++G+ YLE L N V + + + D Y H + +T G Sbjct: 70 PYFTLLLERGASPALYLEGVRLACNFVTVAHGITMLGSISATDAYACFLHGLFLTATGNG 129 Query: 689 EHFD 700 F+ Sbjct: 130 REFE 133 >At1g48390.1 68414.m05405 syntaxin-related family protein contains a novel domain similar to F-box that is shared among other proteins in Arabidopsis; similar to proteins At3g54160, At1g47920 (syntaxin SYP81), At5g41830, At3g44180, At3g58890, At1g56610, At1g48390, At3g59270 [Arabidopsis thaliana] Length = 413 Score = 27.9 bits (59), Expect = 7.1 Identities = 11/21 (52%), Positives = 12/21 (57%) Frame = +1 Query: 145 DGDDSSWDFGVSAGFYLDATN 207 DGD WDF V +Y D TN Sbjct: 235 DGDVYGWDFNVPFDYYGDVTN 255 >At3g20740.1 68416.m02624 fertilization-independent endosperm protein (FIE) contains 6 WD-40 repeats (PF00400); identical to fertilization-independent endosperm protein (GI:4567095) [Arabidopsis thaliana] Length = 369 Score = 27.5 bits (58), Expect = 9.4 Identities = 12/42 (28%), Positives = 20/42 (47%) Frame = -1 Query: 179 LTPKSHDESSPSILTPRGEVSGPTTITPCSAAYLWKPDLVMK 54 +T SH++S I V G T + C +W+ D++ K Sbjct: 328 ITKLSHNQSKSVIRQTAMSVDGSTILACCEDGTIWRWDVITK 369 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,201,277 Number of Sequences: 28952 Number of extensions: 340229 Number of successful extensions: 820 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 792 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 819 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1555552968 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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