Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30468
(703 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative / lysin... 81 5e-16
At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N) f... 33 0.24
At4g40020.1 68417.m05666 hypothetical protein 31 0.74
At2g22270.1 68415.m02644 expressed protein 29 2.3
At5g07660.1 68418.m00877 structural maintenance of chromosomes (... 29 3.9
At1g62480.1 68414.m07050 vacuolar calcium-binding protein-relate... 29 3.9
At4g26870.1 68417.m03867 aspartyl-tRNA synthetase, putative / as... 28 5.2
At5g27300.1 68418.m03260 pentatricopeptide (PPR) repeat-containi... 27 9.1
At1g52430.1 68414.m05919 ubiquitin carboxyl-terminal hydrolase-r... 27 9.1
>At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative /
lysine--tRNA ligase, putative similar to SP|Q43776
Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--tRNA ligase)
{Lycopersicon esculentum}; contains Pfam profile
PF00152: tRNA synthetases class II (D, K and N)
Length = 626
Score = 81.4 bits (192), Expect = 5e-16
Identities = 42/94 (44%), Positives = 62/94 (65%)
Frame = +3
Query: 228 ES*RKVKGEAEKASQQPVVAAEKKPSKQEEEISPNEYYKLRSGAVAALKNGLKEDHPYPH 407
E RK + +AEKA Q P +++K + +EE+ +YY+ R +AA K K ++PYPH
Sbjct: 54 EKRRKDEEKAEKAKQAPKASSQKAVAADDEEMDATQYYENRLKYLAAEK--AKGENPYPH 111
Query: 408 KFNVSISLEEFIEKYQNLNNGDVLENVTLSVAGR 509
KF VS+S+ ++IE Y +LNNGD +EN S+AGR
Sbjct: 112 KFAVSMSIPKYIETYGSLNNGDHVENAEESLAGR 145
Score = 63.3 bits (147), Expect = 1e-10
Identities = 31/66 (46%), Positives = 42/66 (63%), Gaps = 1/66 (1%)
Frame = +2
Query: 509 VHSIRESGAKLIFYDLRAEGAKIQVMASAQLYETED-KFFKDTDKFRRGDIIGCVGHPGK 685
+ S R S +KL FYDL + K+QVMA A ++ +F K +RGDI+G +G PGK
Sbjct: 146 IMSKRSSSSKLFFYDLHGDDFKVQVMADASKSGLDEAEFLKLHSNAKRGDIVGVIGFPGK 205
Query: 686 TKKGEL 703
TK+GEL
Sbjct: 206 TKRGEL 211
>At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N)
family protein similar to SP|Q9RHV9 Lysyl-tRNA
synthetase (EC 6.1.1.6) (Lysine--tRNA ligase) {Bacillus
stearothermophilus}; contains Pfam profile: PF00152 tRNA
synthetases class II (D, K and N)
Length = 602
Score = 32.7 bits (71), Expect = 0.24
Identities = 20/61 (32%), Positives = 31/61 (50%)
Frame = +2
Query: 521 RESGAKLIFYDLRAEGAKIQVMASAQLYETEDKFFKDTDKFRRGDIIGCVGHPGKTKKGE 700
R + KL F LR + IQ+ + ++D+F + GDI+G G +T+KGE
Sbjct: 145 RRAFGKLAFLTLRDDSGTIQLYCEKERL-SDDQFEQLKQFIDIGDILGASGSMKRTEKGE 203
Query: 701 L 703
L
Sbjct: 204 L 204
Score = 28.3 bits (60), Expect = 5.2
Identities = 15/40 (37%), Positives = 24/40 (60%), Gaps = 2/40 (5%)
Frame = +3
Query: 396 PYPHKFNVSISLEEFIEKYQNLNNGDVLENV--TLSVAGR 509
PY +K+ S S + E Y++L NG+ +N +S+AGR
Sbjct: 102 PYAYKWEKSHSANQLQEIYKHLANGEESDNEIDCVSIAGR 141
>At4g40020.1 68417.m05666 hypothetical protein
Length = 615
Score = 31.1 bits (67), Expect = 0.74
Identities = 23/88 (26%), Positives = 40/88 (45%)
Frame = +3
Query: 222 KVES*RKVKGEAEKASQQPVVAAEKKPSKQEEEISPNEYYKLRSGAVAALKNGLKEDHPY 401
K E ++ K E +++ ++ +EKK K+++E + + K G + H +
Sbjct: 403 KKEEKKENKKEKKESKKEKKEHSEKKEDKEKKEQTHQNFDKRMIGKTCSFSIMKLAHHNH 462
Query: 402 PHKFNVSISLEEFIEKYQNLNNGDVLEN 485
HK N S EE +N N G+ EN
Sbjct: 463 NHKHNKETSEEE----TKNANGGNHQEN 486
>At2g22270.1 68415.m02644 expressed protein
Length = 328
Score = 29.5 bits (63), Expect = 2.3
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +1
Query: 175 RCTNLSTAVSKGELKRRLKAEEKLREKPKKLHNSRS 282
R + S S G+ RL +EE + + KK+HN +S
Sbjct: 227 RSSRASVKTSNGQSSNRLFSEEHVVKSSKKIHNQKS 262
>At5g07660.1 68418.m00877 structural maintenance of chromosomes
(SMC) family protein similar to SMC-like protein (MIM)
[Arabidopsis thaliana] GI:5880614; contains Pfam profile
PF02463: RecF/RecN/SMC N terminal domain
Length = 1058
Score = 28.7 bits (61), Expect = 3.9
Identities = 11/27 (40%), Positives = 21/27 (77%)
Frame = +1
Query: 202 SKGELKRRLKAEEKLREKPKKLHNSRS 282
+KGE++ KAE++L+EK +LH++ +
Sbjct: 765 AKGEIEALEKAEDELKEKEDELHSAET 791
>At1g62480.1 68414.m07050 vacuolar calcium-binding protein-related
contains weak similarity to vacuolar calcium binding
protein [Raphanus sativus] gi|9049359|dbj|BAA99394
Length = 152
Score = 28.7 bits (61), Expect = 3.9
Identities = 14/36 (38%), Positives = 22/36 (61%)
Frame = +3
Query: 213 TETKVES*RKVKGEAEKASQQPVVAAEKKPSKQEEE 320
TE VE+ ++ + E +K + PVV E+K + EEE
Sbjct: 100 TEEVVEAKKEEEVEEKKTEEAPVVVEEEKKPEAEEE 135
>At4g26870.1 68417.m03867 aspartyl-tRNA synthetase, putative /
aspartate--tRNA ligase, putative simialr to
aspartate-tRNA ligase (EC 6.1.1.12) from Drosophila
melanogaster GI:4512034, Homo sapiens SP|P14868, Rattus
norvegicus SP|P15178; contains Pfam profile PF00152 tRNA
synthetases class II (D, K and N)
Length = 532
Score = 28.3 bits (60), Expect = 5.2
Identities = 21/64 (32%), Positives = 29/64 (45%), Gaps = 1/64 (1%)
Frame = +2
Query: 509 VHSIRESGAKLIFYDLRAEGAKIQ-VMASAQLYETEDKFFKDTDKFRRGDIIGCVGHPGK 685
+H R G KL F LR G +Q V+ ++ KF K + ++IG V HP K
Sbjct: 94 LHKNRLVGTKL-FVILRESGFTVQCVVEETRVGANMIKFVKQLSRESVVELIGVVSHPKK 152
Query: 686 TKKG 697
G
Sbjct: 153 PLTG 156
>At5g27300.1 68418.m03260 pentatricopeptide (PPR) repeat-containing
protein low similarity to fertility restorer [Petunia x
hybrida] GI:22128587; contains Pfam profile PF01535: PPR
repeat
Length = 510
Score = 27.5 bits (58), Expect = 9.1
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = +1
Query: 169 IGRCTNLSTAVSKGELKRRLKAEEKLREKP 258
+ CT ST+V L+RR+ + +K KP
Sbjct: 83 VSMCTQYSTSVPTRSLRRRISSRKKSSTKP 112
>At1g52430.1 68414.m05919 ubiquitin carboxyl-terminal
hydrolase-related contains Pfam profiles PF00443:
Ubiquitin carboxyl-terminal hydrolase, PF04780: Protein
of unknown function (DUF629), PF04781: Protein of
unknown function (DUF627)
Length = 1136
Score = 27.5 bits (58), Expect = 9.1
Identities = 25/87 (28%), Positives = 44/87 (50%), Gaps = 5/87 (5%)
Frame = +3
Query: 252 EAEKASQQPVVAAEKKPSKQEEEISPNEYYKLRSGAVAALKN---GL--KEDHPYPHKFN 416
E++ AS + +VA+ K + + + + + R G V L++ GL K + K N
Sbjct: 150 ESKIASPETLVASTMKQVSKPKVLESEKIPETRQGVVKRLRSFWVGLDVKVKRDFM-KVN 208
Query: 417 VSISLEEFIEKYQNLNNGDVLENVTLS 497
++ L F+E+ + + DVLENV S
Sbjct: 209 IA-KLLSFVERVRYRDGRDVLENVLAS 234
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,874,278
Number of Sequences: 28952
Number of extensions: 229790
Number of successful extensions: 739
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 709
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 735
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1506636208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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