Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30457
(770 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g61540.1 68418.m07722 L-asparaginase, putative / L-asparagine... 96 3e-20
At5g61540.2 68418.m07723 L-asparaginase, putative / L-asparagine... 48 7e-06
At3g16150.1 68416.m02039 L-asparaginase, putative / L-asparagine... 48 9e-06
At5g08100.1 68418.m00945 L-asparaginase / L-asparagine amidohydr... 44 1e-04
At4g00590.1 68417.m00083 asparaginase 2 family protein very low ... 42 3e-04
At5g08100.2 68418.m00944 L-asparaginase / L-asparagine amidohydr... 40 0.002
At4g12360.1 68417.m01954 protease inhibitor/seed storage/lipid t... 31 1.1
At5g49410.1 68418.m06114 expressed protein similar to unknown pr... 29 4.5
At2g23320.2 68415.m02784 WRKY family transcription factor identi... 29 4.5
At2g23320.1 68415.m02785 WRKY family transcription factor identi... 29 4.5
At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP... 28 7.9
At1g62500.1 68414.m07052 protease inhibitor/seed storage/lipid t... 28 7.9
>At5g61540.1 68418.m07722 L-asparaginase, putative / L-asparagine
amidohydrolase, putative similar to Swiss-Prot:O02467
N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase
(Glycosylasparaginase)
(Aspartylglucosaminidase)(N4-(N-acetyl-beta-
glucosaminyl)-L-asparagine amidase) (AGA)[Spodoptera
frugiperda]
Length = 359
Score = 95.9 bits (228), Expect = 3e-20
Identities = 41/78 (52%), Positives = 58/78 (74%)
Frame = +1
Query: 274 TALDAVEQGASVCEDQQCDGTVGYGGSPDENGETTLDALIMNGRTMNIGAVGGLRRIKHA 453
+A++AV +G S CE+ +CDGTVG GGSPDENGET +DAL+M+G TM +GAV +R +K
Sbjct: 58 SAVEAVVEGCSACEELRCDGTVGPGGSPDENGETMIDALVMDGVTMEVGAVAAMRYVKDG 117
Query: 454 ISVARHVLDHTKHSFLVG 507
I A V+ +++H+ L G
Sbjct: 118 IRAAHLVMKYSQHTLLAG 135
Score = 48.0 bits (109), Expect = 7e-06
Identities = 35/99 (35%), Positives = 49/99 (49%), Gaps = 14/99 (14%)
Frame = +3
Query: 513 ATQFAVEMGFQEE-SLTTSVSKKLWMKWHNEHHCQPNFWLNVSPDPTRNCGPYQKHEYSQ 689
A+ FA+ MG +L++ S K W W E+ CQPNF NV P +CGPY+ + +
Sbjct: 138 ASAFAISMGLPGPMNLSSPESVKKWSDW-KENQCQPNFRKNVV--PANDCGPYKPNNSAM 194
Query: 690 HL-LKKSHLN------------VDRFNHDTIGMVAIDHM 767
++ + KS + V NHDTI M ID M
Sbjct: 195 NVFVDKSTESCEMGAIEYKPPLVGPHNHDTISMAVIDRM 233
Score = 27.9 bits (59), Expect = 7.9
Identities = 10/36 (27%), Positives = 18/36 (50%)
Frame = +2
Query: 146 IFLTYVLVYLTNVCSETNIPIVITTWSFTNSTVKAW 253
+FL+ + V + P+V++TW F + AW
Sbjct: 15 LFLSLLTVADAELVKSDKFPVVVSTWPFLEAVRAAW 50
>At5g61540.2 68418.m07723 L-asparaginase, putative / L-asparagine
amidohydrolase, putative similar to Swiss-Prot:O02467
N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase
(Glycosylasparaginase)
(Aspartylglucosaminidase)(N4-(N-acetyl-beta-
glucosaminyl)-L-asparagine amidase) (AGA)[Spodoptera
frugiperda]
Length = 257
Score = 48.0 bits (109), Expect = 7e-06
Identities = 35/99 (35%), Positives = 49/99 (49%), Gaps = 14/99 (14%)
Frame = +3
Query: 513 ATQFAVEMGFQEE-SLTTSVSKKLWMKWHNEHHCQPNFWLNVSPDPTRNCGPYQKHEYSQ 689
A+ FA+ MG +L++ S K W W E+ CQPNF NV P +CGPY+ + +
Sbjct: 36 ASAFAISMGLPGPMNLSSPESVKKWSDW-KENQCQPNFRKNVV--PANDCGPYKPNNSAM 92
Query: 690 HL-LKKSHLN------------VDRFNHDTIGMVAIDHM 767
++ + KS + V NHDTI M ID M
Sbjct: 93 NVFVDKSTESCEMGAIEYKPPLVGPHNHDTISMAVIDRM 131
>At3g16150.1 68416.m02039 L-asparaginase, putative / L-asparagine
amidohydrolase, putative similar to Swiss-Prot:P30364
L-asparaginase (EC 3.5.1.1) (L-asparagine
amidohydrolase) [Lupinus angustifolius]
Length = 325
Score = 47.6 bits (108), Expect = 9e-06
Identities = 27/76 (35%), Positives = 41/76 (53%)
Frame = +1
Query: 274 TALDAVEQGASVCEDQQCDGTVGYGGSPDENGETTLDALIMNGRTMNIGAVGGLRRIKHA 453
+A+D VE E + G G + E G ++A IM+G GAV G+ +K+
Sbjct: 46 SAIDVVELVIRELETDPLFNS-GRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNP 104
Query: 454 ISVARHVLDHTKHSFL 501
IS+AR V+D + HS+L
Sbjct: 105 ISLARLVMDKSPHSYL 120
>At5g08100.1 68418.m00945 L-asparaginase / L-asparagine
amidohydrolase identical to Swiss-Prot:P50287
L-asparaginase (EC 3.5.1.1) (L-asparagine
amidohydrolase) [Arabidopsis thaliana]
Length = 315
Score = 44.0 bits (99), Expect = 1e-04
Identities = 22/58 (37%), Positives = 32/58 (55%)
Frame = +1
Query: 328 DGTVGYGGSPDENGETTLDALIMNGRTMNIGAVGGLRRIKHAISVARHVLDHTKHSFL 501
D G G G ++A IM+G+T GAV GL + + IS+AR V++ T H +L
Sbjct: 64 DFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 121
>At4g00590.1 68417.m00083 asparaginase 2 family protein very low
similarity to glycosylasparaginase [SP|P20933] from Homo
sapiens; contains Pfam profile PF01112: Asparaginase 2
Length = 408
Score = 42.3 bits (95), Expect = 3e-04
Identities = 27/83 (32%), Positives = 41/83 (49%), Gaps = 1/83 (1%)
Frame = +1
Query: 280 LDAVEQGASVCEDQQCDGTVGYGGSPDENGETTLDALIMNGRTMNIGAVGGLRRIKHAIS 459
+DAV V ED G G + E+G DA +M+G + G VG + +++AI
Sbjct: 55 IDAVSAAIQVLEDDPSTNA-GRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPGVRNAIK 113
Query: 460 VAR-HVLDHTKHSFLVGGLPPNL 525
+A V + S L+G +PP L
Sbjct: 114 IAALLVKEQITGSTLLGRIPPML 136
>At5g08100.2 68418.m00944 L-asparaginase / L-asparagine
amidohydrolase identical to Swiss-Prot:P50287
L-asparaginase (EC 3.5.1.1) (L-asparagine
amidohydrolase) [Arabidopsis thaliana]
Length = 235
Score = 39.9 bits (89), Expect = 0.002
Identities = 18/41 (43%), Positives = 28/41 (68%)
Frame = +1
Query: 379 LDALIMNGRTMNIGAVGGLRRIKHAISVARHVLDHTKHSFL 501
++A IM+G+T GAV GL + + IS+AR V++ T H +L
Sbjct: 1 MEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 41
>At4g12360.1 68417.m01954 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein contains Pfam
protease inhibitor/seed storage/LTP family domain
PF00234
Length = 161
Score = 30.7 bits (66), Expect = 1.1
Identities = 22/80 (27%), Positives = 35/80 (43%), Gaps = 1/80 (1%)
Frame = -1
Query: 506 PTKKECFVWSNTCLATDMACLI-LLKPPTAPIFIVLPFIIRASNVVSPFSSGLPPYPTVP 330
P +EC T L T ++CL +K P +++PF + N + + GL T P
Sbjct: 58 PPSQECCTAVETALTTQLSCLCGFIKSP----MLLIPFNVTDFNALFSKTCGL---TTDP 110
Query: 329 SHCWSSQTDAPCSTASNAVP 270
+ C + AP + VP
Sbjct: 111 NLCSETAAQAPLPKTAAPVP 130
>At5g49410.1 68418.m06114 expressed protein similar to unknown
protein (gb|AAF36742.1)
Length = 145
Score = 28.7 bits (61), Expect = 4.5
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +3
Query: 429 WLEKDQTCHICC*TCIGPHKTFLFSWW 509
W+ + Q + C +GPHK FL++ W
Sbjct: 90 WMRERQKLKLIC-NHVGPHKQFLYTTW 115
>At2g23320.2 68415.m02784 WRKY family transcription factor identical
to WRKY DNA-binding protein 15 GI:13506742 from
[Arabidopsis thaliana]
Length = 262
Score = 28.7 bits (61), Expect = 4.5
Identities = 10/28 (35%), Positives = 17/28 (60%)
Frame = -1
Query: 422 APIFIVLPFIIRASNVVSPFSSGLPPYP 339
AP+ ++ P +++ +PF S LPP P
Sbjct: 99 APVHVISPVLLQEEPKTTPFQSPLPPPP 126
>At2g23320.1 68415.m02785 WRKY family transcription factor identical
to WRKY DNA-binding protein 15 GI:13506742 from
[Arabidopsis thaliana]
Length = 317
Score = 28.7 bits (61), Expect = 4.5
Identities = 10/28 (35%), Positives = 17/28 (60%)
Frame = -1
Query: 422 APIFIVLPFIIRASNVVSPFSSGLPPYP 339
AP+ ++ P +++ +PF S LPP P
Sbjct: 99 APVHVISPVLLQEEPKTTPFQSPLPPPP 126
>At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP2)
identical to biotin carboxyl carrier protein isoform 2
[Arabidopsis thaliana] gi|8886869|gb|AAF80592
Length = 255
Score = 27.9 bits (59), Expect = 7.9
Identities = 17/55 (30%), Positives = 27/55 (49%)
Frame = -1
Query: 428 PTAPIFIVLPFIIRASNVVSPFSSGLPPYPTVPSHCWSSQTDAPCSTASNAVPPL 264
P AP++ +P ++ ++ LPP PT S + T P S S++ PPL
Sbjct: 130 PPAPVYHSMPPVMADFSMPPAQPVALPPSPTPTS---TPATAKPTSAPSSSHPPL 181
>At1g62500.1 68414.m07052 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein similar to auxin
down regulated GB:X69640 GI:296442 from [Glycine max];
contains Pfam profile PF00234: Protease inhibitor/seed
storage/LTP family
Length = 297
Score = 27.9 bits (59), Expect = 7.9
Identities = 15/33 (45%), Positives = 17/33 (51%)
Frame = -1
Query: 440 LLKPPTAPIFIVLPFIIRASNVVSPFSSGLPPY 342
LL P T P ++ P I V P SSG PPY
Sbjct: 163 LLPPVTTPPGLLPPIINPPPVTVPPPSSGYPPY 195
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,245,963
Number of Sequences: 28952
Number of extensions: 372984
Number of successful extensions: 881
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 845
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 881
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1716774400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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