Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV30434
         (400 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein S12|Schizosa...   130   8e-32
SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein S12|Sch...   129   2e-31
SPBC29A3.04 |rpl8||60S ribosomal protein L7a |Schizosaccharomyce...    31   0.066
SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce...    29   0.27 
SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccha...    28   0.61 
SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|ch...    25   4.3  
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig...    24   7.6  
SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharo...    24   10.0 
SPBC13G1.15c |||dubious|Schizosaccharomyces pombe|chr 2|||Manual       24   10.0 
SPCC320.08 |||membrane transporter |Schizosaccharomyces pombe|ch...    24   10.0 
SPAC24H6.01c ||SPAPB21F2.01|membrane bound O-acyltransferase, MB...    24   10.0 

>SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein
           S12|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 148

 Score =  130 bits (314), Expect = 8e-32
 Identities = 58/86 (67%), Positives = 70/86 (81%)
 Frame = +1

Query: 1   ALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLVKVDNNKK 180
           AL+H GL  G+ EA+KALD+RQA LCVL E+CD+ AY KLV+ALC E Q PLVKV + K 
Sbjct: 43  ALVHDGLARGIREASKALDRRQAHLCVLCESCDQEAYVKLVEALCAESQTPLVKVADPKI 102

Query: 181 LGEWAGLCKIDKDGKARKIVGCSCVS 258
           LGEWAGLC +D+DG ARK+VGCSCV+
Sbjct: 103 LGEWAGLCVLDRDGNARKVVGCSCVA 128


>SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein
           S12|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 145

 Score =  129 bits (311), Expect = 2e-31
 Identities = 56/86 (65%), Positives = 70/86 (81%)
 Frame = +1

Query: 1   ALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLVKVDNNKK 180
           AL+H GL  G+ EA+KALD+RQA LCVL E+CD+ AY KLV+ALC E + PL+KV + K 
Sbjct: 40  ALVHDGLARGIREASKALDRRQAHLCVLCESCDQEAYVKLVEALCAESETPLIKVADPKV 99

Query: 181 LGEWAGLCKIDKDGKARKIVGCSCVS 258
           LGEWAGLC +D+DG ARK+VGCSCV+
Sbjct: 100 LGEWAGLCVLDRDGNARKVVGCSCVA 125


>SPBC29A3.04 |rpl8||60S ribosomal protein L7a |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 259

 Score = 31.1 bits (67), Expect = 0.066
 Identities = 14/54 (25%), Positives = 27/54 (50%)
 Frame = +1

Query: 25  HGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLVKVDNNKKLG 186
           +GL+     ++ ++A L ++A + D       + ALC +  +P   V N  +LG
Sbjct: 134 YGLNHVVALIEAKKAKLVLIASDVDPIELVVFLPALCKKMGVPYAIVKNKARLG 187


>SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 154

 Score = 29.1 bits (62), Expect = 0.27
 Identities = 14/57 (24%), Positives = 28/57 (49%)
 Frame = +1

Query: 19  LVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLVKVDNNKKLGE 189
           ++ G+ E  KA+ K +  L +LA +         +  LC ++ +P +   + + LGE
Sbjct: 54  ILRGVKEVVKAVRKGEKGLVILAGDISPMDVISHIPVLCEDNNVPYLYTVSKELLGE 110


>SPAPJ696.01c |vps17||retromer complex subunit
           Vps17|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 549

 Score = 27.9 bits (59), Expect = 0.61
 Identities = 12/34 (35%), Positives = 23/34 (67%), Gaps = 1/34 (2%)
 Frame = -1

Query: 274 SPKSLMTHRSSRQFSLPCHPCQSCRD-QPILQAF 176
           +P S +  ++ +QF LP  PCQ+  + +PI+++F
Sbjct: 220 NPTSGLKRKALKQFPLPPDPCQALANLRPIVKSF 253


>SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 923

 Score = 25.0 bits (52), Expect = 4.3
 Identities = 10/35 (28%), Positives = 19/35 (54%)
 Frame = +2

Query: 83  WLRTVMKPHTRNLFRLCATNIRFHWSRLTTTKSLE 187
           ++ +V  P    L+RLC++  R  W     ++SL+
Sbjct: 220 FILSVFHPDAARLYRLCSSLKRICWELYDPSRSLD 254


>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
           E3 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1647

 Score = 24.2 bits (50), Expect = 7.6
 Identities = 13/34 (38%), Positives = 17/34 (50%), Gaps = 1/34 (2%)
 Frame = +1

Query: 4   LIHGGLVHGLHEAAKALDKRQAVLCVLAEN-CDE 102
           L+H GL+H L  + K          +LA N CDE
Sbjct: 896 LLHSGLIHNLLLSLKKFGSSSLRTFLLAMNTCDE 929


>SPCC63.04 |mok14||alpha-1,3-glucan synthase
           Mok14|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1369

 Score = 23.8 bits (49), Expect = 10.0
 Identities = 12/29 (41%), Positives = 16/29 (55%)
 Frame = +1

Query: 157 VKVDNNKKLGEWAGLCKIDKDGKARKIVG 243
           V+ +N +K  EWAGL  ID +      VG
Sbjct: 527 VRKENKRKAQEWAGL-TIDDEADLLVFVG 554


>SPBC13G1.15c |||dubious|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 111

 Score = 23.8 bits (49), Expect = 10.0
 Identities = 10/22 (45%), Positives = 13/22 (59%)
 Frame = -2

Query: 81  NT*YCLPLVKSFSGFVKTVHKT 16
           N  YCLP   +F  FVK + +T
Sbjct: 90  NRLYCLPFCNAFIEFVKKLTET 111


>SPCC320.08 |||membrane transporter |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 505

 Score = 23.8 bits (49), Expect = 10.0
 Identities = 10/34 (29%), Positives = 19/34 (55%)
 Frame = +2

Query: 143 IRFHWSRLTTTKSLENGLVSARLTRMARQGKLSA 244
           +R+HWS++    +   GLV   ++ +  +G  SA
Sbjct: 258 VRYHWSQILGVVACIGGLVLLVVSDVISRGDYSA 291


>SPAC24H6.01c ||SPAPB21F2.01|membrane bound O-acyltransferase, MBOAT
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 588

 Score = 23.8 bits (49), Expect = 10.0
 Identities = 6/14 (42%), Positives = 11/14 (78%)
 Frame = -1

Query: 118 VSCMRLHHSSQPKH 77
           ++C ++H+ S PKH
Sbjct: 72  IACFKIHYVSSPKH 85


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,434,129
Number of Sequences: 5004
Number of extensions: 25971
Number of successful extensions: 98
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 96
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 98
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 134126124
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)