Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30421
(819 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 158 8e-40
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 153 2e-38
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 93 4e-20
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 61 2e-10
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 28 1.8
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 5.6
SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.4
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 158 bits (384), Expect = 8e-40
Identities = 73/96 (76%), Positives = 82/96 (85%)
Frame = +3
Query: 501 PNLTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKA 680
P+ NLNRLI Q+VSSITASLRF+G+LNVDL EFQTNLVPYPRIHFPLVTYAP++SA KA
Sbjct: 222 PSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKA 281
Query: 681 YHEQLSVAEITNACFEPANQMVKCDPRLASTWLAVC 788
+HE SV EITN CFEP NQMVKCDPR A ++A C
Sbjct: 282 FHESNSVQEITNQCFEPYNQMVKCDPR-AGRYMATC 316
Score = 150 bits (364), Expect = 2e-37
Identities = 63/84 (75%), Positives = 77/84 (91%)
Frame = +2
Query: 2 ETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEI 181
ETG GK+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+
Sbjct: 55 ETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKEL 114
Query: 182 VDLVLDRIRKLADQCTGLQGFLIF 253
VD V D+IR++AD C+GLQGFL+F
Sbjct: 115 VDEVTDKIRRIADNCSGLQGFLVF 138
Score = 126 bits (304), Expect = 4e-30
Identities = 54/72 (75%), Positives = 67/72 (93%)
Frame = +1
Query: 292 LLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYD 471
LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDNE+ YD
Sbjct: 152 LLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYD 211
Query: 472 ICRRNLDIERPT 507
ICRRNLDIERP+
Sbjct: 212 ICRRNLDIERPS 223
Score = 40.3 bits (90), Expect = 3e-04
Identities = 14/21 (66%), Positives = 19/21 (90%)
Frame = +2
Query: 755 PPSGKYMACCMLYRGDVVPKD 817
P +G+YMA C+LYRGDV+P+D
Sbjct: 307 PRAGRYMATCLLYRGDVIPRD 327
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 153 bits (372), Expect = 2e-38
Identities = 71/96 (73%), Positives = 79/96 (82%)
Frame = +3
Query: 501 PNLTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKA 680
P NLNRLI Q+VSSITASLRF G+LNVDL EFQTNLVPYPRIHFPLVTY+P++SA KA
Sbjct: 226 PTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKA 285
Query: 681 YHEQLSVAEITNACFEPANQMVKCDPRLASTWLAVC 788
+HE SV EITN CFEP NQMVKCDPR ++A C
Sbjct: 286 FHESNSVQEITNQCFEPYNQMVKCDPR-TGRYMATC 320
Score = 148 bits (358), Expect = 1e-36
Identities = 60/84 (71%), Positives = 77/84 (91%)
Frame = +2
Query: 2 ETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEI 181
ETG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE+
Sbjct: 59 ETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEM 118
Query: 182 VDLVLDRIRKLADQCTGLQGFLIF 253
+D VL+RIR++AD C+GLQGFL+F
Sbjct: 119 IDSVLERIRRMADNCSGLQGFLVF 142
Score = 133 bits (321), Expect = 3e-32
Identities = 57/72 (79%), Positives = 68/72 (94%)
Frame = +1
Query: 292 LLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYD 471
LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVDNEA YD
Sbjct: 156 LLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYD 215
Query: 472 ICRRNLDIERPT 507
ICRRNLDIERPT
Sbjct: 216 ICRRNLDIERPT 227
Score = 40.3 bits (90), Expect = 3e-04
Identities = 14/21 (66%), Positives = 19/21 (90%)
Frame = +2
Query: 755 PPSGKYMACCMLYRGDVVPKD 817
P +G+YMA C+LYRGDV+P+D
Sbjct: 311 PRTGRYMATCLLYRGDVIPRD 331
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 93.1 bits (221), Expect = 4e-20
Identities = 41/81 (50%), Positives = 55/81 (67%)
Frame = +2
Query: 2 ETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEI 181
E GK+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+
Sbjct: 53 EAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAEL 112
Query: 182 VDLVLDRIRKLADQCTGLQGF 244
D VLD +R+ A+ C LQGF
Sbjct: 113 ADAVLDVVRREAEACDALQGF 133
Score = 80.2 bits (189), Expect = 3e-16
Identities = 34/88 (38%), Positives = 55/88 (62%)
Frame = +3
Query: 498 APNLTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEK 677
+P+ +LN L+ +++ +T S RF G LN DL + N+VP+PR+HF +V +AP+ +
Sbjct: 219 SPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGS 278
Query: 678 AYHEQLSVAEITNACFEPANQMVKCDPR 761
+ + +SV E+T F+ N MV DPR
Sbjct: 279 SSFQAVSVPELTQQMFDANNMMVAADPR 306
Score = 62.1 bits (144), Expect = 9e-11
Identities = 28/72 (38%), Positives = 44/72 (61%)
Frame = +1
Query: 292 LLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYD 471
LL+ ++ +Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +DNEA+
Sbjct: 150 LLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSS 209
Query: 472 ICRRNLDIERPT 507
I L I+ P+
Sbjct: 210 IFANTLKIKSPS 221
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 60.9 bits (141), Expect = 2e-10
Identities = 29/78 (37%), Positives = 49/78 (62%), Gaps = 2/78 (2%)
Frame = +2
Query: 17 KHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDL 190
+++PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I +
Sbjct: 59 RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFED 117
Query: 191 VLDRIRKLADQCTGLQGF 244
++D I + AD L+GF
Sbjct: 118 IMDMIDREADGSDSLEGF 135
Score = 57.6 bits (133), Expect = 2e-09
Identities = 29/98 (29%), Positives = 50/98 (51%), Gaps = 3/98 (3%)
Frame = +3
Query: 501 PNLTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVIS---A 671
P N+L+ ++S+ T +LR+ G +N DL +L+P PR HF L +Y P +
Sbjct: 223 PTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVE 282
Query: 672 EKAYHEQLSVAEITNACFEPANQMVKCDPRLASTWLAV 785
E + +V ++ P NQMV +P S ++++
Sbjct: 283 EAKAIRKTTVLDVMRRLLLPKNQMVSVNPSKKSCFISI 320
Score = 48.0 bits (109), Expect = 2e-06
Identities = 26/72 (36%), Positives = 40/72 (55%), Gaps = 1/72 (1%)
Frame = +1
Query: 295 LMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYD 471
L+ERL+ Y KK ++++P Q VS VV+PYNS+L ++D ++DN A+
Sbjct: 153 LLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAH 212
Query: 472 ICRRNLDIERPT 507
I L + PT
Sbjct: 213 IAADRLHTQNPT 224
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 27.9 bits (59), Expect = 1.8
Identities = 17/49 (34%), Positives = 26/49 (53%)
Frame = +1
Query: 595 PSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTR 741
P+++ P S + T +SS+P P N+ PSP S + AS+ P R
Sbjct: 1232 PTAKAPPVPAPSSEAPSVSTPRSSVPS-PHSNASPSPTSSSMASAAPAR 1279
>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1158
Score = 26.2 bits (55), Expect = 5.6
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +1
Query: 658 QSSLPRRPTMNSFPSPRSQTHASSPPTR 741
+ SLPRRP+ +P S T ++ PP +
Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPPVK 765
>SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 751
Score = 25.8 bits (54), Expect = 7.4
Identities = 16/47 (34%), Positives = 26/47 (55%)
Frame = +1
Query: 592 SPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSP 732
SPS LT L S++ S + + + R+ T +S SP+ Q+ S+P
Sbjct: 619 SPSKMLT-TLRNNSSTFPSLRKNAMIARKSTADSLSSPKRQSVPSTP 664
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,337,521
Number of Sequences: 5004
Number of extensions: 67353
Number of successful extensions: 247
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 210
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 245
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 400438000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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