Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV30370
         (674 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC25H2.02 |ths1||threonine-tRNA ligase Ths1 |Schizosaccharomyc...   112   5e-26
SPAC24C9.09 |||mitochondrial threonine-tRNA ligase|Schizosacchar...    50   4e-07
SPBC106.03 |||DUF1776 family protein|Schizosaccharomyces pombe|c...    25   7.6  
SPBP8B7.21 |ubp3||ubiquitin C-terminal hydrolase Ubp3|Schizosacc...    25   7.6  
SPBC24C6.11 |cwf14||G10 protein|Schizosaccharomyces pombe|chr 2|...    25   10.0 
SPBC56F2.05c |||transcription factor |Schizosaccharomyces pombe|...    25   10.0 
SPCC777.13 |vps35||retromer complex subunit Vps35|Schizosaccharo...    25   10.0 

>SPBC25H2.02 |ths1||threonine-tRNA ligase Ths1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 703

 Score =  112 bits (269), Expect = 5e-26
 Identities = 56/109 (51%), Positives = 74/109 (67%), Gaps = 1/109 (0%)
 Frame = +2

Query: 209 KKNCKRKTAL*TFRTTKEQLLEMFDYNPFKVRILNEKVQT-PTTTVYRXGPLIDLCRGPH 385
           K+  K+K        +KE LLEMF YN +K  I+  K+    +TTVYR GPLIDLC GPH
Sbjct: 174 KQAIKQKQPFERLVISKEGLLEMFRYNKYKQYIIQTKIPDGASTTVYRCGPLIDLCTGPH 233

Query: 386 VRHTGKVKALKVTKNSATYWEGKAEAQSLQRVYGISFPEPKS*KNGRSF 532
           V HTG++K+  VTKNS++Y+ G A+  SLQRVYGISFP+ K  +  ++F
Sbjct: 234 VPHTGRIKSFAVTKNSSSYFLGDAKNDSLQRVYGISFPDNKQMQEYKTF 282



 Score = 86.2 bits (204), Expect = 4e-18
 Identities = 35/56 (62%), Positives = 48/56 (85%)
 Frame = +1

Query: 505 EKLKEWEVIQEEAAKRDHRKIGREQELFFFHELSPGSCFFQPRGAHIYNTLVNFIK 672
           ++++E++    EAAKRDHRKIGR+QELFFF+E+SPGSCFF P GA IYNTL+ +++
Sbjct: 274 KQMQEYKTFLAEAAKRDHRKIGRDQELFFFNEISPGSCFFLPHGARIYNTLLKYMR 329



 Score = 81.4 bits (192), Expect = 1e-16
 Identities = 37/86 (43%), Positives = 58/86 (67%), Gaps = 1/86 (1%)
 Frame = +3

Query: 6   LELLRWDNTDAQAVFWHSSAHMLGEAMERVYGGCLCYGPPIEEGFYYDMYYPE-KGISST 182
           L+L  +++ + + VFWHSSAH+LGEA E  +   LC GPP +EGF+Y+M     + I++ 
Sbjct: 105 LKLCDFNDPEGKRVFWHSSAHILGEATELSFHCHLCIGPPTDEGFFYEMGIDNGRVITND 164

Query: 183 DFNVLEGLIKKIAKEKQPFERLELQR 260
           D++ +E   K+  K+KQPFERL + +
Sbjct: 165 DYSSIESYAKQAIKQKQPFERLVISK 190


>SPAC24C9.09 |||mitochondrial threonine-tRNA
           ligase|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 473

 Score = 49.6 bits (113), Expect = 4e-07
 Identities = 22/47 (46%), Positives = 29/47 (61%)
 Frame = +1

Query: 532 QEEAAKRDHRKIGREQELFFFHELSPGSCFFQPRGAHIYNTLVNFIK 672
           Q E+   DHR I   Q+L+    L+PGS FF P G  IYN LV+F++
Sbjct: 40  QPESTLNDHRTIAARQKLYTTSILTPGSIFFLPHGTRIYNRLVDFLR 86


>SPBC106.03 |||DUF1776 family protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 357

 Score = 25.4 bits (53), Expect = 7.6
 Identities = 11/24 (45%), Positives = 14/24 (58%)
 Frame = +2

Query: 542 PPSVTTERLEGNKSYSSSTNCLPA 613
           PPS+ TER   NK + S   C P+
Sbjct: 289 PPSLCTERKVLNKIFDSLLGCWPS 312


>SPBP8B7.21 |ubp3||ubiquitin C-terminal hydrolase
           Ubp3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 512

 Score = 25.4 bits (53), Expect = 7.6
 Identities = 12/28 (42%), Positives = 17/28 (60%), Gaps = 4/28 (14%)
 Frame = -1

Query: 638 APRGW----KKHEPGDSSWKKNNSCSLP 567
           +P+ W    KKH  GDS  KK++S  +P
Sbjct: 73  SPKSWSAIAKKHVQGDSPVKKSHSVPVP 100


>SPBC24C6.11 |cwf14||G10 protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 146

 Score = 25.0 bits (52), Expect = 10.0
 Identities = 23/63 (36%), Positives = 33/63 (52%), Gaps = 7/63 (11%)
 Frame = +3

Query: 138 FYYDMYYPEKGISSTDFNVL------EGLIKKIAKEKQP-FERLELQRNSYWKCSITIRS 296
           + YD+YY  + IS+  +N L      +G +  IAK K+P +E+L   R     C  T  S
Sbjct: 59  YIYDLYYKREAISTELYNWLLKQNYADGNL--IAKWKKPGYEKLCCLR-----CIQTAES 111

Query: 297 KFG 305
           KFG
Sbjct: 112 KFG 114


>SPBC56F2.05c |||transcription factor |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 397

 Score = 25.0 bits (52), Expect = 10.0
 Identities = 14/35 (40%), Positives = 19/35 (54%)
 Frame = +2

Query: 533 KRRPPSVTTERLEGNKSYSSSTNCLPARVSSSHVE 637
           +RRP    T  L+ + S +S  +  P  VSSS VE
Sbjct: 277 RRRPDHNHTSTLDASSSNTSLASTGPMTVSSSTVE 311


>SPCC777.13 |vps35||retromer complex subunit
           Vps35|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 785

 Score = 25.0 bits (52), Expect = 10.0
 Identities = 11/26 (42%), Positives = 16/26 (61%)
 Frame = +2

Query: 296 KVRILNEKVQTPTTTVYRXGPLIDLC 373
           KVR+L E V T    +Y+ G  ++LC
Sbjct: 530 KVRLLWEFVNTCINVLYKNGDSLELC 555


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,794,512
Number of Sequences: 5004
Number of extensions: 56914
Number of successful extensions: 186
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 184
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)