Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV30370
         (674 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g26830.1 68418.m03201 threonyl-tRNA synthetase / threonine--t...   108   4e-24
At1g17960.1 68414.m02222 threonyl-tRNA synthetase, putative / th...    71   5e-13
At2g04842.1 68415.m00498 threonyl-tRNA synthetase, putative / th...    59   2e-09
At2g41570.1 68415.m05137 hypothetical protein similar to zinc fi...    31   0.93 
At5g03330.2 68418.m00285 OTU-like cysteine protease family prote...    29   3.8  
At5g03330.1 68418.m00284 OTU-like cysteine protease family prote...    29   3.8  
At4g39350.1 68417.m05570 cellulose synthase, catalytic subunit (...    28   6.6  
At5g11520.1 68418.m01344 aspartate aminotransferase, chloroplast...    27   8.7  
At5g10760.1 68418.m01250 aspartyl protease family protein contai...    27   8.7  

>At5g26830.1 68418.m03201 threonyl-tRNA synthetase / threonine--tRNA
           ligase (THRRS) identical to SP|O04630 Threonyl-tRNA
           synthetase, mitochondrial precursor (EC 6.1.1.3)
           (Threonine--tRNA ligase) (ThrRS) {Arabidopsis thaliana}
          Length = 709

 Score =  108 bits (259), Expect = 4e-24
 Identities = 53/118 (44%), Positives = 66/118 (55%)
 Frame = +2

Query: 179 NRFQRTRRANKKNCKRKTAL*TFRTTKEQLLEMFDYNPFKVRILNEKVQTPTTTVYRXGP 358
           N F        K  K          TK+Q LEMF  N FKV ++N      T TVYR GP
Sbjct: 191 NHFPSIEAGAAKAAKEAQPFERIEVTKDQALEMFSENNFKVELINGLPADMTITVYRCGP 250

Query: 359 LIDLCRGPHVRHTGKVKALKVTKNSATYWEGKAEAQSLQRVYGISFPEPKS*KNGRSF 532
           L+DLCRGPH+ +T  VKA K  + S+ YW+G  + +SLQRVYGIS+P+ K  K    F
Sbjct: 251 LVDLCRGPHIPNTSFVKAFKCLRASSAYWKGDKDRESLQRVYGISYPDQKQLKKYLQF 308



 Score = 90.6 bits (215), Expect = 8e-19
 Identities = 43/89 (48%), Positives = 58/89 (65%), Gaps = 2/89 (2%)
 Frame = +3

Query: 3   KLELLRWDNTDAQAVFWHSSAHMLGEAMERVYGGCLCYGPPIE--EGFYYDMYYPEKGIS 176
           KLEL ++D+   +   WHSSAH+LG+A+E+ YG  LC GP     EGFYYD +Y E G+S
Sbjct: 130 KLELFKFDSDKGRDTLWHSSAHILGQALEQEYGCQLCIGPCTTRGEGFYYDGFYGELGLS 189

Query: 177 STDFNVLEGLIKKIAKEKQPFERLELQRN 263
              F  +E    K AKE QPFER+E+ ++
Sbjct: 190 DNHFPSIEAGAAKAAKEAQPFERIEVTKD 218



 Score = 70.1 bits (164), Expect = 1e-12
 Identities = 31/56 (55%), Positives = 42/56 (75%)
 Frame = +1

Query: 505 EKLKEWEVIQEEAAKRDHRKIGREQELFFFHELSPGSCFFQPRGAHIYNTLVNFIK 672
           ++LK++    EEA K DHR +G++QELFF H+LSPGS FF P G  +YN L++FIK
Sbjct: 300 KQLKKYLQFLEEAKKYDHRLLGQKQELFFSHQLSPGSYFFLPLGTRVYNRLMDFIK 355


>At1g17960.1 68414.m02222 threonyl-tRNA synthetase, putative /
           threonine--tRNA ligase, putative similar to SP|O04630
           Threonyl-tRNA synthetase, mitochondrial precursor (EC
           6.1.1.3) (Threonine--tRNA ligase) (ThrRS) {Arabidopsis
           thaliana}; contains Pfam profiles PF00587: tRNA
           synthetase class II core domain (G, H, P, S and T),
           PF03129: Anticodon binding domain, PF02824: TGS domain
          Length = 458

 Score = 71.3 bits (167), Expect = 5e-13
 Identities = 33/68 (48%), Positives = 46/68 (67%), Gaps = 3/68 (4%)
 Frame = +3

Query: 6   LELLRWDNTDAQAVFWHSSAHMLGEAMERVYGGCLCYGP--PIEEGFYYD-MYYPEKGIS 176
           LE+  +D+   +  FWHSSAH+LG+A+E+ YG  LC GP  P +EGFYYD +YY E G++
Sbjct: 100 LEIFGFDSDQGRNTFWHSSAHILGQALEQEYGCKLCIGPCEPRDEGFYYDSLYYGELGLN 159

Query: 177 STDFNVLE 200
              F  +E
Sbjct: 160 DNHFPNIE 167


>At2g04842.1 68415.m00498 threonyl-tRNA synthetase, putative /
           threonine--tRNA ligase, putative similar to SP|P18256
           Threonyl-tRNA synthetase 2 (EC 6.1.1.3) (Threonine--tRNA
           ligase) (ThrRS) {Bacillus subtilis}; contains Pfam
           profiles PF00587: tRNA synthetase class II core domain
           (G, H, P, S and T), PF03129: Anticodon binding domain
          Length = 650

 Score = 59.3 bits (137), Expect = 2e-09
 Identities = 30/84 (35%), Positives = 45/84 (53%), Gaps = 2/84 (2%)
 Frame = +2

Query: 290 PFKVRILNEKVQTPTTTVYRXGPLIDLCRGPHVRHTGKV--KALKVTKNSATYWEGKAEA 463
           P+K+ IL+   + P T  +      DLC GPHV  TGK+  KA+++   +  YW G  + 
Sbjct: 164 PYKMEILDGIKEEPITVYHIGNEWWDLCAGPHVETTGKINKKAVELESVAGAYWRGDEKR 223

Query: 464 QSLQRVYGISFPEPKS*KNGRSFK 535
           Q LQR+YG ++   +  K    FK
Sbjct: 224 QMLQRIYGTAWESEEQLKAYLHFK 247



 Score = 43.6 bits (98), Expect = 1e-04
 Identities = 21/52 (40%), Positives = 33/52 (63%), Gaps = 1/52 (1%)
 Frame = +1

Query: 505 EKLKEWEVIQEEAAKRDHRKIGREQELFFFH-ELSPGSCFFQPRGAHIYNTL 657
           E+LK +   +EEA +RDHR+IG++ +LF    E   G  F+ P+GA + N +
Sbjct: 238 EQLKAYLHFKEEAKRRDHRRIGQDLDLFSIQDEAGGGLVFWHPKGAIVRNII 289



 Score = 35.5 bits (78), Expect = 0.033
 Identities = 21/94 (22%), Positives = 45/94 (47%), Gaps = 1/94 (1%)
 Frame = +3

Query: 24  DNTDAQAVFWHSSAHMLGEAMERVYGGC-LCYGPPIEEGFYYDMYYPEKGISSTDFNVLE 200
           +++D      H+ AH++  A+++++    +  GP I+ GFYYD  +  + ++  D   ++
Sbjct: 74  ESSDKLLKIRHTCAHVMAMAVQKLFPDAKVTIGPWIDNGFYYD--FDMEPLTDKDLKRIK 131

Query: 201 GLIKKIAKEKQPFERLELQRNSYWKCSITIRSKF 302
             + +I     P  R E+ R    K  + I   +
Sbjct: 132 KEMDRIISRNLPLLREEVSREEAKKRIMAINEPY 165


>At2g41570.1 68415.m05137 hypothetical protein similar to zinc
           finger protein [Arabidopsis thaliana] GI:976277
          Length = 418

 Score = 30.7 bits (66), Expect = 0.93
 Identities = 13/28 (46%), Positives = 19/28 (67%)
 Frame = +2

Query: 545 PSVTTERLEGNKSYSSSTNCLPARVSSS 628
           PS   +R+EGN + S+ TN L +R+ SS
Sbjct: 257 PSSDLDRIEGNSALSTKTNLLNSRIMSS 284


>At5g03330.2 68418.m00285 OTU-like cysteine protease family protein
           contains Pfam profile PF02338: OTU-like cysteine
           protease
          Length = 356

 Score = 28.7 bits (61), Expect = 3.8
 Identities = 15/47 (31%), Positives = 23/47 (48%), Gaps = 3/47 (6%)
 Frame = +1

Query: 523 EVIQEEAAKRDHRKIGREQELFFFHEL---SPGSCFFQPRGAHIYNT 654
           E+  EE A  DH ++    E+F F E+     G+C F+     +Y T
Sbjct: 190 EIPPEEEAVSDHERLRNRLEMFDFTEVKVPGDGNCQFRALADQLYKT 236


>At5g03330.1 68418.m00284 OTU-like cysteine protease family protein
           contains Pfam profile PF02338: OTU-like cysteine
           protease
          Length = 356

 Score = 28.7 bits (61), Expect = 3.8
 Identities = 15/47 (31%), Positives = 23/47 (48%), Gaps = 3/47 (6%)
 Frame = +1

Query: 523 EVIQEEAAKRDHRKIGREQELFFFHEL---SPGSCFFQPRGAHIYNT 654
           E+  EE A  DH ++    E+F F E+     G+C F+     +Y T
Sbjct: 190 EIPPEEEAVSDHERLRNRLEMFDFTEVKVPGDGNCQFRALADQLYKT 236


>At4g39350.1 68417.m05570 cellulose synthase, catalytic subunit
           (Ath-A) identical to gi:2827141
          Length = 1084

 Score = 27.9 bits (59), Expect = 6.6
 Identities = 10/28 (35%), Positives = 14/28 (50%)
 Frame = -3

Query: 474 CKLCASALPSQYVAEFFVTFNALTFPVC 391
           C++C   +     +E FV  N   FPVC
Sbjct: 39  CQICGDEIELTVSSELFVACNECAFPVC 66


>At5g11520.1 68418.m01344 aspartate aminotransferase, chloroplast /
           transaminase A (ASP3) (YLS4) identical to SP|P46644
           Aspartate aminotransferase, chloroplast precursor (EC
           2.6.1.1) (Transaminase A) {Arabidopsis thaliana};
           identical to cDNA YLS4 mRNA for aspartate
           aminotransferase (ASP3), partial cds GI:13122285
          Length = 449

 Score = 27.5 bits (58), Expect = 8.7
 Identities = 19/63 (30%), Positives = 29/63 (46%)
 Frame = +1

Query: 466 ELTARVRYIISRTEKLKEWEVIQEEAAKRDHRKIGREQELFFFHELSPGSCFFQPRGAHI 645
           EL A    IIS  ++L  +E ++      D   I ++  +F F  L+P    F  +  HI
Sbjct: 359 ELKAMADRIISMRKQL--FEALRTRGTPGDWSHIIKQIGMFTFTGLNPAQVSFMTKEYHI 416

Query: 646 YNT 654
           Y T
Sbjct: 417 YMT 419


>At5g10760.1 68418.m01250 aspartyl protease family protein contains
           Pfam domain, PF00026: eukaryotic aspartyl protease
          Length = 464

 Score = 27.5 bits (58), Expect = 8.7
 Identities = 15/45 (33%), Positives = 27/45 (60%), Gaps = 1/45 (2%)
 Frame = -3

Query: 558 VVTLG-GLLLNDLPFFQLFGSGNDIPYTRCKLCASALPSQYVAEF 427
           +VT+G G   +DL    +F +G+D+ +T+C+ C  +  SQ   +F
Sbjct: 133 IVTIGIGTPKHDLSL--VFDTGSDLTWTQCEPCLGSCYSQKEPKF 175


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,829,802
Number of Sequences: 28952
Number of extensions: 306547
Number of successful extensions: 906
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 872
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 903
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1432596384
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)