Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30358
(756 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g30630.1 68414.m03746 coatomer protein epsilon subunit family... 65 5e-11
At2g34840.1 68415.m04278 coatomer protein epsilon subunit family... 60 1e-09
At1g58440.1 68414.m06648 squalene monooxygenase, putative / squa... 30 1.9
At2g40130.2 68415.m04936 heat shock protein-related contains sim... 29 3.3
At2g40130.1 68415.m04935 heat shock protein-related contains sim... 29 3.3
>At1g30630.1 68414.m03746 coatomer protein epsilon subunit family
protein / COPE family protein similar to SP|O14579
Coatomer epsilon subunit (Epsilon-coat protein)
(Epsilon-COP) from Homo sapiens, SP|Q60445 from
Cricetulus griseus; ESTs gb|Z17908, gb|AA728673,
gb|N96555, gb|H76335, gb|AA712463, gb|W43247, gb|T45611,
gb|T21160, gb|T14119 and AI100483 come from this gene
Length = 292
Score = 64.9 bits (151), Expect = 5e-11
Identities = 29/74 (39%), Positives = 48/74 (64%)
Frame = +2
Query: 287 VAKGTELSNEIFLIVAATIYYHEDNYEAALKILHNAESLELRAFTLQCLLAMNRPDLARK 466
+A T +N I ++A TI+ HE++Y ALK H+ +++L A +Q + M+R D A K
Sbjct: 98 LADPTVGNNAIIRLIAGTIFMHEEDYNEALKHTHSGGTMDLHALNVQIFIKMHRSDFAEK 157
Query: 467 QLKLLQDIEDDGTL 508
QL+++Q I++D TL
Sbjct: 158 QLRVMQQIDEDHTL 171
Score = 54.0 bits (124), Expect = 1e-07
Identities = 29/74 (39%), Positives = 48/74 (64%), Gaps = 1/74 (1%)
Frame = +3
Query: 18 DELFDVKNAFYVGNYQQAINEAQSVSPSTPLVALQRDAFLYRSYIAQGNYRIVQQEL-KT 194
D LF+++N FY+G YQ AIN ++ + S + ++RD ++R+YIA G+Y++V E+ +
Sbjct: 8 DHLFNLRNHFYLGAYQAAINNSEIPNLSQEDI-VERDCLVHRAYIALGSYQLVISEIDEA 66
Query: 195 ADPMLQPLKSLVDY 236
A LQ +K L Y
Sbjct: 67 AATPLQAVKLLAMY 80
Score = 33.1 bits (72), Expect = 0.20
Identities = 15/28 (53%), Positives = 18/28 (64%)
Frame = +1
Query: 511 QLAQAWLNLIQGGPGIQDAHYSVMELSE 594
QLA AWLNL GG IQ+A+ + SE
Sbjct: 173 QLASAWLNLAVGGSKIQEAYLIFQDFSE 200
>At2g34840.1 68415.m04278 coatomer protein epsilon subunit family
protein / COPE family protein similar to SP|O14579
Coatomer epsilon subunit (Epsilon-coat protein)
(Epsilon-COP) from Homo sapiens, SP|Q60445 from
Cricetulus griseus
Length = 293
Score = 60.5 bits (140), Expect = 1e-09
Identities = 31/74 (41%), Positives = 51/74 (68%), Gaps = 1/74 (1%)
Frame = +3
Query: 18 DELFDVKNAFYVGNYQQAINEAQSVSPSTPLVALQRDAFLYRSYIAQGNYRIVQQEL-KT 194
D LF+++N FY+G YQ AIN ++ ++ +P A++RD ++RSYIA G+Y++V E+ ++
Sbjct: 9 DHLFNLRNNFYLGAYQTAINNSE-IANLSPENAVERDCLVFRSYIALGSYQLVISEIDES 67
Query: 195 ADPMLQPLKSLVDY 236
A LQ +K L Y
Sbjct: 68 AATPLQAVKLLAMY 81
Score = 57.6 bits (133), Expect = 8e-09
Identities = 28/99 (28%), Positives = 55/99 (55%)
Frame = +2
Query: 212 AIEEFGRLLTT*CQ*SAIVADIDARVAKGTELSNEIFLIVAATIYYHEDNYEAALKILHN 391
A++ L+T + ++ + +A T +N+ ++A I+ HE++Y LK H
Sbjct: 74 AVKLLAMYLSTPQNKESTISSLKEWLADSTIGNNDTLRLIAGIIFMHEEDYNETLKHTHA 133
Query: 392 AESLELRAFTLQCLLAMNRPDLARKQLKLLQDIEDDGTL 508
+++L A +Q + M+R + A KQL+++Q I++D TL
Sbjct: 134 GGTMDLYALNVQIFIKMHRAEYAEKQLRVMQQIDEDHTL 172
Score = 32.3 bits (70), Expect = 0.36
Identities = 15/28 (53%), Positives = 18/28 (64%)
Frame = +1
Query: 511 QLAQAWLNLIQGGPGIQDAHYSVMELSE 594
QLA AWLNL GG IQ+A+ + SE
Sbjct: 174 QLASAWLNLAVGGSKIQEAYLIFEDFSE 201
>At1g58440.1 68414.m06648 squalene monooxygenase, putative /
squalene epoxidase, putative similar to SP|O65404 (SE
1,1), SP|O65402 (SE 1,2) 6566341 dbj AB008021.1 AB008021
Length = 531
Score = 29.9 bits (64), Expect = 1.9
Identities = 18/61 (29%), Positives = 27/61 (44%), Gaps = 1/61 (1%)
Frame = +3
Query: 54 GNYQQAINEAQSVSP-STPLVALQRDAFLYRSYIAQGNYRIVQQELKTADPMLQPLKSLV 230
GN + N + SP TP L DAF R + G + ++ +L+PL+ L
Sbjct: 322 GNIKSMPNRSMPASPYPTPGALLMGDAFNMRHPLTGGGMTVALADIVVLRNLLRPLRDLS 381
Query: 231 D 233
D
Sbjct: 382 D 382
>At2g40130.2 68415.m04936 heat shock protein-related contains
similarity to 101 kDa heat shock protein; HSP101
[Triticum aestivum] gi|11561808|gb|AAC83689
Length = 910
Score = 29.1 bits (62), Expect = 3.3
Identities = 18/74 (24%), Positives = 34/74 (45%), Gaps = 1/74 (1%)
Frame = -3
Query: 511 AQSAVIFNVLQQLQLFACKVWSIHSQ*ALECERS*FQRF-SIMKDF*CSFIIIFMIVNCC 335
A + ++ + + L+ +VW I + + E +RF ++ KD+ + I + C
Sbjct: 330 AANYIVNRISELLRRHGRRVWLIGATTSNEVYEKMMRRFPNVEKDWDLQLLTITSLKPCL 389
Query: 334 SHNQEYFIGQFRTF 293
HN+ IG F F
Sbjct: 390 PHNKSSLIGSFVPF 403
>At2g40130.1 68415.m04935 heat shock protein-related contains
similarity to 101 kDa heat shock protein; HSP101
[Triticum aestivum] gi|11561808|gb|AAC83689
Length = 491
Score = 29.1 bits (62), Expect = 3.3
Identities = 18/74 (24%), Positives = 34/74 (45%), Gaps = 1/74 (1%)
Frame = -3
Query: 511 AQSAVIFNVLQQLQLFACKVWSIHSQ*ALECERS*FQRF-SIMKDF*CSFIIIFMIVNCC 335
A + ++ + + L+ +VW I + + E +RF ++ KD+ + I + C
Sbjct: 330 AANYIVNRISELLRRHGRRVWLIGATTSNEVYEKMMRRFPNVEKDWDLQLLTITSLKPCL 389
Query: 334 SHNQEYFIGQFRTF 293
HN+ IG F F
Sbjct: 390 PHNKSSLIGSFVPF 403
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,820,390
Number of Sequences: 28952
Number of extensions: 285277
Number of successful extensions: 668
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 654
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 666
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1682736544
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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