Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30350
(589 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to ... 100 1e-21
At5g19180.1 68418.m02284 ubiquitin activating enzyme, putative (... 31 0.75
At5g06460.1 68418.m00724 ubiquitin activating enzyme 2 (UBA2) E1... 29 1.7
At4g38870.1 68417.m05504 F-box family protein contains Pfam prof... 29 2.3
At2g30110.1 68415.m03664 ubiquitin activating enzyme 1 (UBA1) E1... 29 3.0
At2g21470.2 68415.m02555 SUMO activating enzyme 2 (SAE2) nearly ... 29 3.0
At2g21470.1 68415.m02554 SUMO activating enzyme 2 (SAE2) nearly ... 29 3.0
At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam d... 27 7.0
At1g55300.1 68414.m06317 TATA-binding protein-associated factor ... 27 7.0
At1g51720.1 68414.m05828 glutamate dehydrogenase, putative simil... 27 7.0
At5g13610.1 68418.m01576 expressed protein 27 9.3
At4g17610.1 68417.m02633 tRNA/rRNA methyltransferase (SpoU) fami... 27 9.3
At3g49410.1 68416.m05401 transcription factor-related contains w... 27 9.3
At2g28610.1 68415.m03478 homeobox-leucine zipper transcription f... 27 9.3
At1g04620.1 68414.m00456 coenzyme F420 hydrogenase family / dehy... 27 9.3
>At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to
autophagy 7 [Arabidopsis thaliana] GI:19912147; contains
Pfam profile PF00899: ThiF family
Length = 697
Score = 99.5 bits (237), Expect = 1e-21
Identities = 48/92 (52%), Positives = 61/92 (66%)
Frame = +1
Query: 232 STGHQASWSAGWVGWERNDKGNFGPRLANMSTSMDPVILADTSSDLNIKLMKWRLVPDLN 411
S+G A VGWE N KG PR +++ SMDP LA ++ DLN+KLM+WR +P LN
Sbjct: 293 SSGESAETVPNSVGWELN-KGKRVPRSISLANSMDPTRLAVSAVDLNLKLMRWRALPSLN 351
Query: 412 VGVMKDTKCLLLGAGTLGCHVARNLLALGFRH 507
+ V+ KCLLLGAGTLGC VAR L+ G R+
Sbjct: 352 LNVLSSVKCLLLGAGTLGCQVARTLMGWGIRN 383
Score = 41.9 bits (94), Expect = 3e-04
Identities = 17/27 (62%), Positives = 21/27 (77%)
Frame = +2
Query: 506 INFRDNGKVSYSNPTRQVLFNYQDCLG 586
I F D GKV+ SNP RQ L+N++DCLG
Sbjct: 384 ITFVDYGKVAMSNPVRQSLYNFEDCLG 410
Score = 29.9 bits (64), Expect = 1.3
Identities = 13/24 (54%), Positives = 16/24 (66%), Gaps = 1/24 (4%)
Frame = +2
Query: 59 FVFADPSN-GCNPGWPLRTFLAAL 127
F F DP + NPGWPLR +LA +
Sbjct: 234 FGFYDPCHLPSNPGWPLRNYLALI 257
>At5g19180.1 68418.m02284 ubiquitin activating enzyme, putative
(ECR1) identical to putative ubiquitin activating enzyme
E1 [Arabidopsis thaliana] GI:2952433; similar to NEDD8
activating enzyme [Mus musculus] GI:17061821
Length = 454
Score = 30.7 bits (66), Expect = 0.75
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = +1
Query: 400 PDLNVGVMKDTKCLLLGAGTLGCHVARNLLALGFRH 507
P L + + L++GAG LGC + ++L GFR+
Sbjct: 37 PGLRDDIRDYVRILVIGAGGLGCELLKDLALSGFRN 72
>At5g06460.1 68418.m00724 ubiquitin activating enzyme 2 (UBA2) E1;
identical to gi:1703477
Length = 1077
Score = 29.5 bits (63), Expect = 1.7
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = +1
Query: 421 MKDTKCLLLGAGTLGCHVARNLLALG 498
++D + ++GAG LGC +NL +G
Sbjct: 487 LEDARVFVVGAGALGCEFLKNLALMG 512
>At4g38870.1 68417.m05504 F-box family protein contains Pfam
profile: PF00646 F-box domain
Length = 426
Score = 29.1 bits (62), Expect = 2.3
Identities = 16/54 (29%), Positives = 25/54 (46%)
Frame = -2
Query: 288 IVSFPSYPTSAPTGLMSCGISMEKTRLFMKSPFIELLNPITCKVDFAKSGRYSN 127
I+ PSY S+ + + C + K R F+K + E N + C D S + N
Sbjct: 371 IIFTPSYAHSSLSSAIYCDLKENKVRKFVKEGWTE--NYLPCNADSVSSTQVEN 422
>At2g30110.1 68415.m03664 ubiquitin activating enzyme 1 (UBA1) E1;
identical to GB:U80808
Length = 1080
Score = 28.7 bits (61), Expect = 3.0
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = +1
Query: 421 MKDTKCLLLGAGTLGCHVARNLLALG 498
++D K +G+G LGC +NL +G
Sbjct: 490 LEDAKVFTVGSGALGCEFLKNLALMG 515
>At2g21470.2 68415.m02555 SUMO activating enzyme 2 (SAE2) nearly
identical to SUMO activating enzyme 2 [Arabidopsis
thaliana] GI:22652854; contains Pfam profiles PF00899:
ThiF family, PF02134: Repeat in ubiquitin-activating
(UBA) protein
Length = 700
Score = 28.7 bits (61), Expect = 3.0
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +1
Query: 421 MKDTKCLLLGAGTLGCHVARNLLALGF 501
+K K L++GAG +GC + + L GF
Sbjct: 10 IKGAKVLMVGAGGIGCELLKTLALSGF 36
>At2g21470.1 68415.m02554 SUMO activating enzyme 2 (SAE2) nearly
identical to SUMO activating enzyme 2 [Arabidopsis
thaliana] GI:22652854; contains Pfam profiles PF00899:
ThiF family, PF02134: Repeat in ubiquitin-activating
(UBA) protein
Length = 625
Score = 28.7 bits (61), Expect = 3.0
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +1
Query: 421 MKDTKCLLLGAGTLGCHVARNLLALGF 501
+K K L++GAG +GC + + L GF
Sbjct: 10 IKGAKVLMVGAGGIGCELLKTLALSGF 36
>At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam
domain PF04931: DNA polymerase V
Length = 1306
Score = 27.5 bits (58), Expect = 7.0
Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
Frame = -3
Query: 392 RHFINFIFRSDDVSA-NMTGSIEVDILASLGPKLPLSFLSH 273
R+F D +S+ ++ + DIL L PKLP SF+ H
Sbjct: 445 RNFCEVFMEGDLLSSTHVRKHLAFDILLLLLPKLPASFIQH 485
>At1g55300.1 68414.m06317 TATA-binding protein-associated factor
TAFII55 family protein contains Pfam profile: PF04658
TAFII55 protein conserved region
Length = 203
Score = 27.5 bits (58), Expect = 7.0
Identities = 14/51 (27%), Positives = 24/51 (47%)
Frame = -3
Query: 467 QPSVPAPNNKHFVSFITPTFRSGTRRHFINFIFRSDDVSANMTGSIEVDIL 315
+P PAPN + +TP + +R F R D++ + +E D+L
Sbjct: 89 EPGDPAPNTVEYRHGLTPPMKDARKRRF----RREPDLNPELVQRVERDLL 135
>At1g51720.1 68414.m05828 glutamate dehydrogenase, putative similar
to NADP-specific glutatamate dehydrogenase (NADP-GDH)
SP:P28724 [Giardia lamblia (Giardia intestinalis)]
Length = 637
Score = 27.5 bits (58), Expect = 7.0
Identities = 19/71 (26%), Positives = 35/71 (49%), Gaps = 1/71 (1%)
Frame = +1
Query: 289 KGNF-GPRLANMSTSMDPVILADTSSDLNIKLMKWRLVPDLNVGVMKDTKCLLLGAGTLG 465
+G+F GPR+ ++S L +S + ++ D+N + K +C++ G G +
Sbjct: 376 QGSFTGPRIYWAASS-----LRTEASGYGVVYFARLILADMNKEI-KGLRCVVSGCGKIA 429
Query: 466 CHVARNLLALG 498
HV L+A G
Sbjct: 430 MHVVEKLIACG 440
>At5g13610.1 68418.m01576 expressed protein
Length = 402
Score = 27.1 bits (57), Expect = 9.3
Identities = 18/52 (34%), Positives = 25/52 (48%)
Frame = +3
Query: 378 VDEMASST*SECRCDERYEMFVIGSRHTWLSRGEKFISLGFPS*TFVTTVKC 533
V+ AS E R DE YE+ + TW+ G FI L F + + T+ C
Sbjct: 207 VERHASGLLPEMRKDE-YEVRENPNLDTWMEVGRDFIRLQFLNTDGIRTIGC 257
>At4g17610.1 68417.m02633 tRNA/rRNA methyltransferase (SpoU) family
protein similar to TAR RNA loop binding protein [Homo
sapiens] GI:1184692; contains Pfam profile PF00588: SpoU
rRNA Methylase (RNA methyltransferase, TrmH) family
Length = 1850
Score = 27.1 bits (57), Expect = 9.3
Identities = 16/38 (42%), Positives = 21/38 (55%)
Frame = -2
Query: 240 SCGISMEKTRLFMKSPFIELLNPITCKVDFAKSGRYSN 127
+C SM +T F+ PFIE LN T DF G Y++
Sbjct: 521 TCTQSMSQT--FVLGPFIEGLNDPTHHKDFGLKGIYTS 556
>At3g49410.1 68416.m05401 transcription factor-related contains weak
similarity to transcription factor IIIC63 (GI:5281316)
[Homo sapiens]
Length = 559
Score = 27.1 bits (57), Expect = 9.3
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = -2
Query: 303 SKIAFIVSFPSYPTSAPTGLMSCG 232
SK AF+V FP YP+S + + G
Sbjct: 15 SKEAFVVHFPGYPSSISRAIETLG 38
>At2g28610.1 68415.m03478 homeobox-leucine zipper transcription
factor (PRESSED FLOWER) identical to PRESSED FLOWER
(GP:17907768) {Arabidopsis thaliana}
Length = 244
Score = 27.1 bits (57), Expect = 9.3
Identities = 12/44 (27%), Positives = 24/44 (54%)
Frame = -2
Query: 276 PSYPTSAPTGLMSCGISMEKTRLFMKSPFIELLNPITCKVDFAK 145
PSYP+S+ + + C + M ++ +K+ + ++ I K D K
Sbjct: 200 PSYPSSSSSPISCCNMMMSSPKIPLKTLELFPISSINSKQDSTK 243
>At1g04620.1 68414.m00456 coenzyme F420 hydrogenase family /
dehydrogenase, beta subunit family contains Pfam
PF04432: Coenzyme F420 hydrogenase/dehydrogenase, beta
domain; similar to Coenzyme F420 hydrogenase beta
subunit (SP:Q00391) {Methanobacterium
thermoautotrophicum}
Length = 462
Score = 27.1 bits (57), Expect = 9.3
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = -3
Query: 422 ITPTFRSGTRRHFINFIFRSDDVS 351
ITPT SG RR F+ ++DD +
Sbjct: 354 ITPTISSGDRRPFVTETVKADDAA 377
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,655,737
Number of Sequences: 28952
Number of extensions: 300512
Number of successful extensions: 829
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 812
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 829
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1161268208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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