Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV30343
         (506 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein S12|Sch...    79   4e-16
SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein S12|Schizosa...    79   5e-16
SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence orphan|Schizos...    28   0.70 
SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces p...    27   1.6  
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    27   2.1  
SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase Ub...    25   4.9  
SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccha...    25   6.5  
SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    25   6.5  
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch...    25   8.6  
SPBC2G2.08 |ade9||C-1-tetrahydrofolatesynthase/methylenetetrahyd...    25   8.6  

>SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein
           S12|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 145

 Score = 79.0 bits (186), Expect = 4e-16
 Identities = 36/58 (62%), Positives = 45/58 (77%)
 Frame = +3

Query: 78  MDVNVALQEVLKTALIHGGLVHGLHETAKALDKRQAVLCVLAENCDEAAYKKLVQALC 251
           + V  AL+EVLK AL+H GL  G+ E +KALD+RQA LCVL E+CD+ AY KLV+ALC
Sbjct: 27  VSVEDALKEVLKRALVHDGLARGIREASKALDRRQAHLCVLCESCDQEAYVKLVEALC 84



 Score = 77.0 bits (181), Expect = 2e-15
 Identities = 31/54 (57%), Positives = 42/54 (77%)
 Frame = +2

Query: 254 QHQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVL 415
           + + PL+KV + K LGEWAGLC +D+DG ARK+VGCSCV + D+GE++ AL  L
Sbjct: 86  ESETPLIKVADPKVLGEWAGLCVLDRDGNARKVVGCSCVAVTDYGEDSAALQKL 139


>SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein
           S12|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 148

 Score = 78.6 bits (185), Expect = 5e-16
 Identities = 33/54 (61%), Positives = 42/54 (77%)
 Frame = +2

Query: 254 QHQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVL 415
           + Q PLVKV + K LGEWAGLC +D+DG ARK+VGCSCV + D+GE++ AL  L
Sbjct: 89  ESQTPLVKVADPKILGEWAGLCVLDRDGNARKVVGCSCVAVTDYGEDSVALQTL 142



 Score = 78.2 bits (184), Expect = 7e-16
 Identities = 35/58 (60%), Positives = 45/58 (77%)
 Frame = +3

Query: 78  MDVNVALQEVLKTALIHGGLVHGLHETAKALDKRQAVLCVLAENCDEAAYKKLVQALC 251
           + V  +L+EVLK AL+H GL  G+ E +KALD+RQA LCVL E+CD+ AY KLV+ALC
Sbjct: 30  LSVEDSLKEVLKRALVHDGLARGIREASKALDRRQAHLCVLCESCDQEAYVKLVEALC 87


>SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence
           orphan|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1563

 Score = 28.3 bits (60), Expect = 0.70
 Identities = 22/77 (28%), Positives = 35/77 (45%), Gaps = 4/77 (5%)
 Frame = -2

Query: 220 ASSQFSAKTHSTACLLSRALAVS*RPCTR----PPWIKAVFSTSCSATFTSMLLPDKMGL 53
           ASS F A T ST+C  +  L V+    T     PP    + +   + T T++  P  +  
Sbjct: 157 ASSTFVADTTSTSCNPATVLIVTTSGSTSTSCPPPTTILIVTVPTTTTTTTVGYPGSVTT 216

Query: 52  VGTSTSTSAMVVDYLTV 2
             T T ++  V+D + V
Sbjct: 217 TLTGTPSNGTVIDTVEV 233


>SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 404

 Score = 27.1 bits (57), Expect = 1.6
 Identities = 10/25 (40%), Positives = 14/25 (56%)
 Frame = -2

Query: 403 RWSFLAEIFDDNTGAADNFPCLAIL 329
           RW +LA +F  N  A  N P + +L
Sbjct: 257 RWEYLASLFTSNFTAVHNIPSVPLL 281


>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 800

 Score = 26.6 bits (56), Expect = 2.1
 Identities = 18/74 (24%), Positives = 31/74 (41%)
 Frame = -2

Query: 238 TSFLYAASSQFSAKTHSTACLLSRALAVS*RPCTRPPWIKAVFSTSCSATFTSMLLPDKM 59
           TS  Y ++   S    +T C  S ++  +  P T  P      +TS S  +TS  +    
Sbjct: 454 TSVPYTSTPVTSTPLTTTNCTTSTSIPYTSTPVTSTPLTTTNCTTSTSVPYTSTPVTSSN 513

Query: 58  GLVGTSTSTSAMVV 17
             + +ST  ++  V
Sbjct: 514 YTISSSTPVTSTPV 527



 Score = 24.6 bits (51), Expect = 8.6
 Identities = 19/71 (26%), Positives = 30/71 (42%), Gaps = 1/71 (1%)
 Frame = -2

Query: 238 TSFLYAASSQFSAKTHSTACLLSRALAVS*RPCTRPPWIKAVFSTSCSATFTSM-LLPDK 62
           TS  Y ++   S    +T C  S ++  +  P T  P      +TS S  +TS  +    
Sbjct: 431 TSVPYTSTPVTSTPLATTNCTTSTSVPYTSTPVTSTPLTTTNCTTSTSIPYTSTPVTSTP 490

Query: 61  MGLVGTSTSTS 29
           +     +TSTS
Sbjct: 491 LTTTNCTTSTS 501


>SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase
           Ubp21|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1129

 Score = 25.4 bits (53), Expect = 4.9
 Identities = 14/38 (36%), Positives = 20/38 (52%)
 Frame = +3

Query: 39  VEVPTNPILSGNNMDVNVALQEVLKTALIHGGLVHGLH 152
           +E+   P LS    D + +   VL   L+HGG +HG H
Sbjct: 445 LEIDLEPYLS-ETADKSESHVYVLHGVLVHGGDLHGGH 481


>SPAPJ696.01c |vps17||retromer complex subunit
           Vps17|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 549

 Score = 25.0 bits (52), Expect = 6.5
 Identities = 10/28 (35%), Positives = 20/28 (71%), Gaps = 1/28 (3%)
 Frame = -1

Query: 371 QHRSSRQFSLPCHPCQSCRD-QPILQAF 291
           + ++ +QF LP  PCQ+  + +PI+++F
Sbjct: 226 KRKALKQFPLPPDPCQALANLRPIVKSF 253


>SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 821

 Score = 25.0 bits (52), Expect = 6.5
 Identities = 11/32 (34%), Positives = 15/32 (46%)
 Frame = +3

Query: 213 DEAAYKKLVQALCNNIRFHWSRLTTTKSLENG 308
           DE      V  L + ++  W R TT  S E+G
Sbjct: 14  DEPTTSSRVSMLVDRVKLLWKRYTTPLSTEDG 45


>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1133

 Score = 24.6 bits (51), Expect = 8.6
 Identities = 9/20 (45%), Positives = 14/20 (70%)
 Frame = -3

Query: 135 DHHGLKLSLAPLAVLHSRPC 76
           D  GL  ++  L+++HSRPC
Sbjct: 529 DEMGLGKTIEVLSLIHSRPC 548


>SPBC2G2.08 |ade9||C-1-
           tetrahydrofolatesynthase/methylenetetrahydrofolatedehydr
           ogenase/methylenetetrahydrofolatecyclohydrolase/formylte
           trahydrofolatesynthetase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 969

 Score = 24.6 bits (51), Expect = 8.6
 Identities = 11/20 (55%), Positives = 15/20 (75%)
 Frame = +3

Query: 345 GKLSAAPVLSSKISARKLQR 404
           G  S+A +L+ K+ ARKLQR
Sbjct: 28  GTSSSANILNGKLLARKLQR 47


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,978,792
Number of Sequences: 5004
Number of extensions: 37775
Number of successful extensions: 140
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 140
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 202220600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)