Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30315
(686 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g12870.1 68417.m02015 gamma interferon responsive lysosomal t... 40 0.001
At4g12900.1 68417.m02018 gamma interferon responsive lysosomal t... 38 0.005
At2g44580.1 68415.m05548 zinc finger (C3HC4-type RING finger) fa... 38 0.008
At4g12960.1 68417.m02025 gamma interferon responsive lysosomal t... 36 0.033
At1g07080.1 68414.m00754 gamma interferon responsive lysosomal t... 35 0.044
At4g12890.1 68417.m02017 gamma interferon responsive lysosomal t... 34 0.10
At5g01580.1 68418.m00073 gamma interferon responsive lysosomal t... 33 0.18
At4g21020.1 68417.m03041 late embryogenesis abundant domain-cont... 30 1.3
At1g56510.1 68414.m06498 disease resistance protein (TIR-NBS-LRR... 30 1.3
At4g38350.1 68417.m05422 patched family protein similar to SP|O1... 30 1.7
At2g36910.1 68415.m04527 multidrug resistance P-glycoprotein (PG... 30 1.7
At1g42470.1 68414.m04897 patched family protein similar to SP|O1... 29 2.2
At1g15130.1 68414.m01807 hydroxyproline-rich glycoprotein family... 29 2.2
At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DN... 28 5.0
At3g18290.1 68416.m02326 zinc finger protein-related weak alignm... 28 5.0
At5g39440.1 68418.m04777 Snf1-related protein kinase, putative s... 28 6.7
At4g37060.1 68417.m05248 patatin, putative similar to patatin-li... 28 6.7
At1g06490.1 68414.m00688 glycosyl transferase family 48 protein ... 28 6.7
At5g51750.1 68418.m06417 subtilase family protein similar to sub... 27 8.8
At4g00416.1 68417.m00056 methyl-CpG-binding domain-containing pr... 27 8.8
>At4g12870.1 68417.m02015 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 229
Score = 40.3 bits (90), Expect = 0.001
Identities = 24/76 (31%), Positives = 37/76 (48%)
Frame = +3
Query: 243 LQRTVKVKMVPYGKSTHDKVDGKWSFICHHGADECYGNKVQACVLKDRNLQDTEKMEIVI 422
L VK+VP+G + KV + IC HG +EC N ++ACV+ + + +
Sbjct: 62 LDTITDVKLVPFG---YAKVSNNLTVICQHGEEECKLNALEACVINTLP-NPKSQYKFIR 117
Query: 423 CLMNQTSPDKSLDTCL 470
C+ N T D +CL
Sbjct: 118 CVENNT--DNWESSCL 131
Score = 36.3 bits (80), Expect = 0.019
Identities = 18/50 (36%), Positives = 28/50 (56%), Gaps = 2/50 (4%)
Frame = +1
Query: 94 RLVCFLMLLCYVVAKKKT--EDHKVKIAVYYESLCPDSKKFITTQLAPVW 237
+LV F + + + K E KV++ +YYESLCP + FI +L V+
Sbjct: 9 KLVFFACFVLFTFSHKLVTGESDKVELNLYYESLCPGCQSFIVDELVKVF 58
>At4g12900.1 68417.m02018 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 231
Score = 38.3 bits (85), Expect = 0.005
Identities = 23/72 (31%), Positives = 34/72 (47%), Gaps = 4/72 (5%)
Frame = +1
Query: 94 RLVCFLMLLCYVVAKKKT---EDHKVKIAVYYESLCPDSKKFITTQLAPVWR-DFRGLSK 261
+LV F LL + + E KVK+ +YYESLCP + FI L ++ D ++
Sbjct: 12 KLVFFACLLLFTFSSHNLVAGESDKVKLNLYYESLCPSCQNFIVHHLGKIFNTDLHTITD 71
Query: 262 SKWSRMGRARTT 297
K G A +
Sbjct: 72 LKLIPFGNAHVS 83
Score = 31.9 bits (69), Expect = 0.41
Identities = 13/46 (28%), Positives = 24/46 (52%)
Frame = +3
Query: 243 LQRTVKVKMVPYGKSTHDKVDGKWSFICHHGADECYGNKVQACVLK 380
L +K++P+G + V + C HG +EC N ++AC ++
Sbjct: 66 LHTITDLKLIPFGNA---HVSDDLTVTCQHGEEECKLNALEACAIR 108
>At2g44580.1 68415.m05548 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 624
Score = 37.5 bits (83), Expect = 0.008
Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 1/72 (1%)
Frame = +3
Query: 297 KVDGKWSFICHHGADECYGNKVQACVLKDRNLQDTEKMEIVICLMNQTSPDKSLDTCLTQ 476
++DG W I + D + CVLKD + D ++ E+V L+ P + CL
Sbjct: 194 EIDGFWRVIDENYLDVILRMLLHNCVLKDWSFDDLDEDEVVNALVADEFPSQLASHCLRV 253
Query: 477 V-DKMSESDKLK 509
K++E+DK K
Sbjct: 254 FGSKVNETDKWK 265
>At4g12960.1 68417.m02025 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 233
Score = 35.5 bits (78), Expect = 0.033
Identities = 19/50 (38%), Positives = 29/50 (58%), Gaps = 2/50 (4%)
Frame = +1
Query: 94 RLVCF--LMLLCYVVAKKKTEDHKVKIAVYYESLCPDSKKFITTQLAPVW 237
+LV F L+LL + + KVK+ +YYESLCP ++FI L ++
Sbjct: 8 KLVFFGCLLLLTFTDNLVAGKSGKVKLNLYYESLCPGCQEFIVDDLGKIF 57
Score = 31.1 bits (67), Expect = 0.72
Identities = 17/61 (27%), Positives = 29/61 (47%), Gaps = 1/61 (1%)
Frame = +3
Query: 201 QEVYNDATGSRLE-GLQRTVKVKMVPYGKSTHDKVDGKWSFICHHGADECYGNKVQACVL 377
QE D G + L +K+ P+G + ++ + C HG +EC N ++AC L
Sbjct: 46 QEFIVDDLGKIFDYDLYTITDLKLFPFGNA---ELSDNLTVTCQHGEEECKLNALEACAL 102
Query: 378 K 380
+
Sbjct: 103 R 103
>At1g07080.1 68414.m00754 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 265
Score = 35.1 bits (77), Expect = 0.044
Identities = 14/29 (48%), Positives = 18/29 (62%)
Frame = +1
Query: 157 KVKIAVYYESLCPDSKKFITTQLAPVWRD 243
KV + +YYESLCP FI LA ++ D
Sbjct: 38 KVSVGLYYESLCPYCSSFIVNHLAKLFED 66
Score = 31.1 bits (67), Expect = 0.72
Identities = 16/55 (29%), Positives = 28/55 (50%)
Frame = +2
Query: 512 CASSEQGDNLLASYGDKTDAVMRPLAFVPTVIINEKYDKDVETQAFENLKAVVCR 676
C SS G+ L Y +T+A+ P +VP V++ D + +EN + +C+
Sbjct: 155 CLSSGHGNELALHYAAETNALQPPHKYVPWVVV----DGQPLYEDYENFISYICK 205
Score = 30.3 bits (65), Expect = 1.3
Identities = 18/70 (25%), Positives = 35/70 (50%), Gaps = 4/70 (5%)
Frame = +3
Query: 321 ICHHGADECYGNKVQACVLKDRNLQDTEKMEIVICLMNQTSPDK--SLDTCLTQVDKMSE 494
+C HGA EC+ + V+AC + D + ++ + C+ + K +TC +++ S+
Sbjct: 92 VCQHGAFECFLDTVEACAI-DAWPKVSDHFPFIYCVEKLVTEHKYDKWETCYEKLNLNSK 150
Query: 495 --SDKLKDAH 518
+D L H
Sbjct: 151 PVADCLSSGH 160
>At4g12890.1 68417.m02017 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 232
Score = 33.9 bits (74), Expect = 0.10
Identities = 14/28 (50%), Positives = 19/28 (67%)
Frame = +1
Query: 154 HKVKIAVYYESLCPDSKKFITTQLAPVW 237
+KVKI +YYESLCP + FI L ++
Sbjct: 37 NKVKINLYYESLCPYCQNFIVDDLGKIF 64
Score = 33.5 bits (73), Expect = 0.13
Identities = 18/68 (26%), Positives = 35/68 (51%), Gaps = 1/68 (1%)
Frame = +3
Query: 243 LQRTVKVKMVPYGKSTHDKVDGKWSFICHHGADECYGNKVQACVLKDRNLQDTE-KMEIV 419
L + +K+VP+G + + + C HG +EC N ++AC + R L D + + + +
Sbjct: 68 LLKITDLKLVPFGNA---HISNNLTITCQHGEEECKLNALEACGI--RTLPDPKLQYKFI 122
Query: 420 ICLMNQTS 443
C+ T+
Sbjct: 123 RCVEKDTN 130
>At5g01580.1 68418.m00073 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 233
Score = 33.1 bits (72), Expect = 0.18
Identities = 15/46 (32%), Positives = 27/46 (58%)
Frame = +3
Query: 240 GLQRTVKVKMVPYGKSTHDKVDGKWSFICHHGADECYGNKVQACVL 377
GL ++ +++VP+G + + DG + +C HG EC N + AC +
Sbjct: 55 GLISSIDLQLVPWGNAAI-RPDG--TILCQHGEAECALNAIHACAI 97
Score = 32.3 bits (70), Expect = 0.31
Identities = 13/52 (25%), Positives = 26/52 (50%)
Frame = +1
Query: 82 MKTLRLVCFLMLLCYVVAKKKTEDHKVKIAVYYESLCPDSKKFITTQLAPVW 237
M + + +C + + KV +++YYE+LCP +FI +L ++
Sbjct: 1 MASYQRLCITSCTIFFCLLSLSSSQKVTLSLYYEALCPFCAEFIVNRLPKIF 52
>At4g21020.1 68417.m03041 late embryogenesis abundant
domain-containing protein / LEA domain-containing
protein low similarity to SP|P23283 Desiccation-related
protein {Craterostigma plantagineum}; contains Pfam
profile PF02987: Late embryogenesis abundant protein
Length = 266
Score = 30.3 bits (65), Expect = 1.3
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = +3
Query: 210 YNDATGSRLEGLQRTVKVKMVPYGKSTHDKVDGKW 314
Y + T + EG + TVK K G+ T + V G W
Sbjct: 179 YGEDTKEKAEGFKETVKGKAEELGEKTKETVKGAW 213
>At1g56510.1 68414.m06498 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1007
Score = 30.3 bits (65), Expect = 1.3
Identities = 16/51 (31%), Positives = 28/51 (54%), Gaps = 3/51 (5%)
Frame = +3
Query: 375 LKDRNLQDTEKMEI---VICLMNQTSPDKSLDTCLTQVDKMSESDKLKDAH 518
L + N+QD+E ++ CL N D S +CLT++ +S + L+D +
Sbjct: 603 LVEVNMQDSELQKLWEGTQCLANLKKIDLSRSSCLTELPDLSNATNLEDLY 653
>At4g38350.1 68417.m05422 patched family protein similar to
SP|O15118 Niemann-Pick C1 protein precursor from Homo
sapiens (PID:g2276463); contains Pfam profile PF02460
Patched family
Length = 1064
Score = 29.9 bits (64), Expect = 1.7
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = +1
Query: 100 VCFLMLLCYVVAKKKTEDHKVKIAVYYESLCPDSKKFITTQLAPVWR 240
V ++ LC + K E K+ V ES + KKF T L+P +R
Sbjct: 315 VAIVLALCSGLYNFKVETRPEKLWVGPESKAAEEKKFFDTHLSPFYR 361
>At2g36910.1 68415.m04527 multidrug resistance P-glycoprotein (PGP1)
identical to P-glycoprotein GI:3849833 from [Arabidopsis
thaliana]; homologous to mammalian mdr gene,contains
ATP-binding cassette; related to multi drug resistance
proteins
Length = 1286
Score = 29.9 bits (64), Expect = 1.7
Identities = 18/66 (27%), Positives = 31/66 (46%)
Frame = -3
Query: 252 SSEVPPDGSQLRRYKLLAVRTKGFVIDRYFDLMVFGFLLRHDIAEQHQKANQPESLHLVV 73
S V DG +R+Y L A+R ++ + + +FG + +IA H+ A + E +
Sbjct: 1080 SGRVMIDGKDIRKYNLKAIRKHIAIVPQ--EPCLFGTTIYENIAYGHECATEAEIIQAAT 1137
Query: 72 LYQEKK 55
L K
Sbjct: 1138 LASAHK 1143
>At1g42470.1 68414.m04897 patched family protein similar to
SP|O15118 Niemann-Pick C1 protein precursor from Homo
sapiens (GB:AAB63982) (GI:2276463); contains Pfam
profile PF02460 Patched family
Length = 1272
Score = 29.5 bits (63), Expect = 2.2
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = +1
Query: 100 VCFLMLLCYVVAKKKTEDHKVKIAVYYESLCPDSKKFITTQLAPVWR 240
V ++LLC + + K E K+ V S + K+F T LAP +R
Sbjct: 356 VSVVLLLCVGLIRFKVETRPDKLWVGSGSRAAEEKQFFDTHLAPFYR 402
>At1g15130.1 68414.m01807 hydroxyproline-rich glycoprotein family
protein
Length = 846
Score = 29.5 bits (63), Expect = 2.2
Identities = 12/31 (38%), Positives = 15/31 (48%)
Frame = +2
Query: 359 GASLRPEGPQPPGHGEDGDCNMPHEPDQPRQ 451
G S+ P GP PP H G P+ P +Q
Sbjct: 776 GYSIPPYGPPPPYHTPHGQAPQPYPPQAQQQ 806
>At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DNA
topoisomerase II / DNA gyrase (TOP2) identical to
SP|P30182 DNA topoisomerase II (EC 5.99.1.3) {Arabidopsis
thaliana}
Length = 1473
Score = 28.3 bits (60), Expect = 5.0
Identities = 22/80 (27%), Positives = 32/80 (40%)
Frame = +3
Query: 390 LQDTEKMEIVICLMNQTSPDKSLDTCLTQVDKMSESDKLKDAHPVNKETICSRRTVIKRM 569
L D +KM I + M +T+P + L +DK E LKDA ++ K
Sbjct: 1137 LADRDKMIIAVADMKKTTPKSLWLSDLESLDKELEKLDLKDAQVQQAIEAAQKKIRAKSG 1196
Query: 570 L**DLSRSCPPLLLTRNTTK 629
+ R P + TTK
Sbjct: 1197 AAVKVKRQAPKKPAPKKTTK 1216
>At3g18290.1 68416.m02326 zinc finger protein-related weak alignment
to Pfam profiles: PF00097 Zinc finger, C3HC4 type (RING
finger) (2 copies)
Length = 1254
Score = 28.3 bits (60), Expect = 5.0
Identities = 13/44 (29%), Positives = 19/44 (43%)
Frame = -2
Query: 205 SCCQDKGIRNRPLF*PYGLRFSSSPRHSRAASESKPAGESSSCC 74
SCCQ + R ++P + A+ KP+G SCC
Sbjct: 563 SCCQHQDKRPAKRTAVLSCEKKTTPHSTEVANGCKPSGNGRSCC 606
>At5g39440.1 68418.m04777 Snf1-related protein kinase, putative
similar to SNF1-related protein kinase KIN10 (EC
2.7.1.-) (AKIN10) [Arabidopsis thaliana]
SWISS-PROT:Q38997
Length = 494
Score = 27.9 bits (59), Expect = 6.7
Identities = 11/53 (20%), Positives = 26/53 (49%)
Frame = -3
Query: 198 VRTKGFVIDRYFDLMVFGFLLRHDIAEQHQKANQPESLHLVVLYQEKKRLYDF 40
++ G I ++ + FL+ I Q++ P +++V+ Y + L+D+
Sbjct: 55 IKNMGIEIKVQREIKILRFLMHPHIIRQYEVIETPNDIYVVMEYVKSGELFDY 107
>At4g37060.1 68417.m05248 patatin, putative similar to patatin-like
latex allergen [Hevea brasiliensis][PMID:10589016];
contains patatin domain PF01734
Length = 414
Score = 27.9 bits (59), Expect = 6.7
Identities = 20/67 (29%), Positives = 30/67 (44%), Gaps = 7/67 (10%)
Frame = -3
Query: 621 YFSLIITVGTNARGLITASVLSP-------YDASRLSPCSLDAHLSVYPTPTSCPLELST 463
YF +I GT+ GL+TA + +P + A + P L+ ++P PT L
Sbjct: 58 YFDVI--AGTSTGGLVTAMLTAPDENGRPRFAAKEIVPFYLEHCPKIFPQPTGVLALLPK 115
Query: 462 CPKTCRG 442
PK G
Sbjct: 116 LPKLLSG 122
>At1g06490.1 68414.m00688 glycosyl transferase family 48 protein
contains Pfam profile: PF02364 1,3-beta-glucan synthase
Length = 1933
Score = 27.9 bits (59), Expect = 6.7
Identities = 17/69 (24%), Positives = 33/69 (47%), Gaps = 7/69 (10%)
Frame = +1
Query: 118 LCYVVAKKKTEDHKVKIA-VY------YESLCPDSKKFITTQLAPVWRDFRGLSKSKWSR 276
LCY+ E H + VY YE+ PD + F+ + P+++ R + + ++
Sbjct: 363 LCYIFHNMANEVHGILFGNVYPVTGDTYEAGAPDEEAFLRNVITPIYQVLR--KEVRRNK 420
Query: 277 MGRARTTRW 303
G+A ++W
Sbjct: 421 NGKASHSKW 429
>At5g51750.1 68418.m06417 subtilase family protein similar to
subtilisin-like protease GI:3687307 from [Lycopersicon
esculentum]
Length = 780
Score = 27.5 bits (58), Expect = 8.8
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +1
Query: 139 KKTEDHKVKIAVYYESLCPDSKKFITTQLAPVWRDFRG 252
++ DH V + V + P+S+ F T ++PV +RG
Sbjct: 141 ERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRG 178
>At4g00416.1 68417.m00056 methyl-CpG-binding domain-containing
protein contains Pfam profile PF01429: Methyl-CpG
binding domain
Length = 163
Score = 27.5 bits (58), Expect = 8.8
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = +1
Query: 160 VKIAVYYESLCPDSKKF 210
VK+ VYYESL P K+F
Sbjct: 90 VKVDVYYESLAPRRKRF 106
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,256,310
Number of Sequences: 28952
Number of extensions: 354901
Number of successful extensions: 1139
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1093
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1139
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1457719448
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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