Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30302X
(490 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 47 6e-06
At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1 (e... 47 8e-06
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 46 2e-05
At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2 (e... 44 7e-05
At2g40660.1 68415.m05017 tRNA-binding region domain-containing p... 32 0.24
At1g32830.1 68414.m04046 hypothetical protein similar to At2g049... 31 0.32
At4g13290.1 68417.m02078 cytochrome P450 71A19, putative (CYP71A... 27 5.1
At2g14140.1 68415.m01575 hypothetical protein similar to At2g049... 27 6.8
At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 27 6.8
At5g34895.1 68418.m04113 hypothetical protein similar to At2g049... 27 9.0
At3g30450.1 68416.m03852 hypothetical protein similar to At2g049... 27 9.0
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 47.2 bits (107), Expect = 6e-06
Identities = 19/58 (32%), Positives = 34/58 (58%)
Frame = +3
Query: 39 AVGDVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQGFEQVGKAPAANLPHVLRWYNQI 212
A ++ + GL L+++L +SY++GY S+ D+ F + K P + +V RW+N I
Sbjct: 3 AFPNLNSGSGLKKLDEHLLTRSYITGYQASKDDITVFTALSKPPTSEFVNVSRWFNHI 60
Score = 38.3 bits (85), Expect = 0.003
Identities = 15/27 (55%), Positives = 20/27 (74%)
Frame = +2
Query: 410 KKSKKPALIAKSSILLDVQPWDDDTDM 490
K S K KSS+L+D++PWDD+TDM
Sbjct: 132 KASTKKKESGKSSVLMDIKPWDDETDM 158
>At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1
(eEF1Balpha1) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686819
Length = 228
Score = 46.8 bits (106), Expect = 8e-06
Identities = 21/58 (36%), Positives = 35/58 (60%)
Frame = +3
Query: 48 DVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQGFEQVGKAPAANLPHVLRWYNQIASY 221
D+ T +GL L ++LA K+Y+SG S DV+ + V + P P+ +WY+ +AS+
Sbjct: 7 DLHTERGLKTLEEHLAGKTYISGDQLSVDDVKVYAAVLENPGDGFPNASKWYDSVASH 64
Score = 39.1 bits (87), Expect = 0.002
Identities = 16/27 (59%), Positives = 21/27 (77%)
Frame = +2
Query: 410 KKSKKPALIAKSSILLDVQPWDDDTDM 490
K +KK KSS+LL+V+PWDD+TDM
Sbjct: 129 KDTKKTKESGKSSVLLEVKPWDDETDM 155
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 45.6 bits (103), Expect = 2e-05
Identities = 19/58 (32%), Positives = 33/58 (56%)
Frame = +3
Query: 39 AVGDVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQGFEQVGKAPAANLPHVLRWYNQI 212
A ++ + GL L+++L +SY++GY S+ D+ F + K P + + RWYN I
Sbjct: 3 AFPNLNSDAGLKKLDEHLLTRSYITGYQASKDDITVFAALAKPPTSQYVNASRWYNHI 60
Score = 38.3 bits (85), Expect = 0.003
Identities = 15/27 (55%), Positives = 20/27 (74%)
Frame = +2
Query: 410 KKSKKPALIAKSSILLDVQPWDDDTDM 490
K S K KSS+L+D++PWDD+TDM
Sbjct: 132 KASTKKKESGKSSVLIDIKPWDDETDM 158
>At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2
(eEF1Balpha2) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686821
Length = 224
Score = 43.6 bits (98), Expect = 7e-05
Identities = 19/57 (33%), Positives = 33/57 (57%)
Frame = +3
Query: 48 DVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQGFEQVGKAPAANLPHVLRWYNQIAS 218
D+ T +G+ + ++LA K+Y+SG S DV+ + V P+ P+ +WY +AS
Sbjct: 7 DLHTEEGVKSVEEHLAGKTYISGDQLSVDDVKVYAAVPVKPSDAFPNASKWYESVAS 63
Score = 43.2 bits (97), Expect = 1e-04
Identities = 17/27 (62%), Positives = 22/27 (81%)
Frame = +2
Query: 410 KKSKKPALIAKSSILLDVQPWDDDTDM 490
K +KKP KSS+L+DV+PWDD+TDM
Sbjct: 125 KDTKKPKESGKSSVLMDVKPWDDETDM 151
>At2g40660.1 68415.m05017 tRNA-binding region domain-containing
protein similar to SP|Q12904 Multisynthetase complex
auxiliary component p43 [Contains: Endothelial-monocyte
activating polypeptide II (EMAP-II) (Small inducible
cytokine subfamily E member 1)] {Homo sapiens}; contains
Pfam profile PF01588: Putative tRNA binding domain
Length = 389
Score = 31.9 bits (69), Expect = 0.24
Identities = 22/57 (38%), Positives = 28/57 (49%), Gaps = 9/57 (15%)
Frame = +3
Query: 69 LNDLNQYLAEKSYV--SGYTPSQADVQGFEQV-------GKAPAANLPHVLRWYNQI 212
L LN LA KS + +G TPS ADV F + + +PHV+RW N I
Sbjct: 76 LEKLNLELATKSVLLGNGLTPSAADVAVFSALHSSVLGLSDSDKEKVPHVIRWVNYI 132
>At1g32830.1 68414.m04046 hypothetical protein similar to At2g04970,
At2g15200, At2g14140, At3g30450, At4g03990, At5g34895,
At3g47270, At2g02200
Length = 707
Score = 31.5 bits (68), Expect = 0.32
Identities = 21/68 (30%), Positives = 33/68 (48%), Gaps = 1/68 (1%)
Frame = +3
Query: 108 VSGYTPSQAD-VQGFEQVGKAPAANLPHVLRWYNQIASYTSAERKTWSQGPAH*PPVLNP 284
+ GY S+ D QG+ QV + + HVL+ + + S +RK+ S G N
Sbjct: 334 IKGYRMSKKDWCQGWIQVEQGHQGRIDHVLKPAIETVVHQSRKRKSMSFGKKATKGQKNK 393
Query: 285 RLPRQQRE 308
R+P+Q E
Sbjct: 394 RIPKQGDE 401
>At4g13290.1 68417.m02078 cytochrome P450 71A19, putative (CYP71A19)
Identical to Cytochrome P450 (SP:Q9T0K0) [Arabidopsis
thaliana]; similar to cytochrome P450LXXIA1, Persea
americana, M32885
Length = 490
Score = 27.5 bits (58), Expect = 5.1
Identities = 18/51 (35%), Positives = 23/51 (45%), Gaps = 2/51 (3%)
Frame = +1
Query: 109 CLDTLLLKLMFKDLSKLARRRPLTFP--TYYDGIIKLLHTLRLNAKHGLRA 255
CL TLL L+ K + K L P + +I LH L LN LR+
Sbjct: 9 CLTTLLALLLLKSILKRTTTNNLNLPPSPWRLPVIGNLHQLSLNTHRSLRS 59
>At2g14140.1 68415.m01575 hypothetical protein similar to At2g04970,
At2g15200, At1g32830, At3g30450, At4g03990, At5g34895,
At3g47270, At2g02200
Length = 847
Score = 27.1 bits (57), Expect = 6.8
Identities = 16/50 (32%), Positives = 26/50 (52%), Gaps = 1/50 (2%)
Frame = +3
Query: 108 VSGYTPSQAD-VQGFEQVGKAPAANLPHVLRWYNQIASYTSAERKTWSQG 254
+ GY S+ D QG+ QV + + HVL+ + + S +RK+ S G
Sbjct: 334 IKGYRMSKKDWCQGWIQVEQGHQGRIDHVLKSAIETVVHQSRKRKSMSFG 383
>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
/ VPS11 family protein similar to Vacuolar protein
sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
sapiens]; similar to Vacuolar biogenesis protein END1
(PEP5 protein) (Vacuolar protein sorting 11)
(Swiss-Prot:P12868) [Saccharomyces cerevisiae]
Length = 932
Score = 27.1 bits (57), Expect = 6.8
Identities = 16/32 (50%), Positives = 16/32 (50%)
Frame = +3
Query: 69 LNDLNQYLAEKSYVSGYTPSQADVQGFEQVGK 164
L DL Y YVS PSQA V EQ GK
Sbjct: 495 LEDLGNYDEALQYVSSLEPSQAGVT-IEQYGK 525
>At5g34895.1 68418.m04113 hypothetical protein similar to At2g04970,
At2g15200, At1g32830, At2g14140, At3g30450, At4g03990,
At3g47270, At2g02200
Length = 490
Score = 26.6 bits (56), Expect = 9.0
Identities = 16/50 (32%), Positives = 26/50 (52%), Gaps = 1/50 (2%)
Frame = +3
Query: 108 VSGYTPSQAD-VQGFEQVGKAPAANLPHVLRWYNQIASYTSAERKTWSQG 254
+ GY S+ D QG+ QV + + HVL+ + + S +RK+ S G
Sbjct: 168 IKGYRMSKKDWCQGWIQVEQGHQGRIDHVLKPAIETVVHQSRKRKSMSFG 217
>At3g30450.1 68416.m03852 hypothetical protein similar to At2g04970,
At2g15200, At1g32830, At2g14140, At4g03990, At5g34895,
At3g47270, At2g02200
Length = 800
Score = 26.6 bits (56), Expect = 9.0
Identities = 16/50 (32%), Positives = 26/50 (52%), Gaps = 1/50 (2%)
Frame = +3
Query: 108 VSGYTPSQAD-VQGFEQVGKAPAANLPHVLRWYNQIASYTSAERKTWSQG 254
+ GY S+ D QG+ QV + + HVL+ + + S +RK+ S G
Sbjct: 322 IKGYRMSKKDWCQGWIQVEQGHQGRIDHVLKPAIETVVHQSRKRKSMSFG 371
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,609,954
Number of Sequences: 28952
Number of extensions: 141834
Number of successful extensions: 349
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 344
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 349
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 848837888
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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