Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30265X
(502 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-... 146 1e-35
At3g26560.1 68416.m03315 ATP-dependent RNA helicase, putative si... 70 8e-13
At1g32490.1 68414.m04009 RNA helicase, putative similar to ATP-d... 63 9e-11
At4g16680.1 68417.m02519 RNA helicase, putative similar to SP|Q1... 62 2e-10
At2g35340.1 68415.m04333 RNA helicase, putative similar to ATP-d... 62 2e-10
At1g26370.1 68414.m03217 RNA helicase, putative similar to SP|Q1... 59 1e-09
At3g62310.1 68416.m07000 RNA helicase, putative similar to SP|P5... 55 2e-08
At2g47250.1 68415.m05900 RNA helicase, putative similar to SP|P5... 55 3e-08
At5g14900.1 68418.m01748 helicase associated (HA2) domain-contai... 52 2e-07
At4g18465.1 68417.m02740 RNA helicase, putative similar to SP|Q1... 52 2e-07
At1g27900.1 68414.m03419 RNA helicase, putative similar to SP|Q1... 45 3e-05
At1g33390.1 68414.m04133 helicase domain-containing protein simi... 44 6e-05
At5g10370.1 68418.m01203 helicase domain-containing protein / IB... 44 8e-05
At4g01020.1 68417.m00137 helicase domain-containing protein / IB... 41 4e-04
At1g78700.1 68414.m09173 brassinosteroid signalling positive reg... 29 1.8
At5g47210.1 68418.m05821 nuclear RNA-binding protein, putative s... 28 3.1
At5g18410.2 68418.m02167 expressed protein similar to p53 induci... 28 4.1
At5g18410.1 68418.m02166 expressed protein similar to p53 induci... 28 4.1
At3g24650.1 68416.m03095 abscisic acid-insensitive protein 3 (AB... 27 5.4
At2g33360.1 68415.m04089 expressed protein 27 9.4
>At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-box
RNA helicase [Chlamydomonas reinhardtii] GI:12044832;
contains Pfam profiles PF04408: Helicase associated
domain (HA2), PF00271: Helicase conserved C-terminal
domain
Length = 1226
Score = 146 bits (353), Expect = 1e-35
Identities = 61/90 (67%), Positives = 74/90 (82%)
Frame = -3
Query: 491 YFQQAARLKGIGEYVNCRTGMPCHLHPTSALFGLGCSPDYVVYHELTMTAREYMHCVTAV 312
YF +ARLKG+GEYVNCRTGMPCHLHP+SAL+GLG +PDYVVYHEL +T +EYM C T+V
Sbjct: 1079 YFHNSARLKGVGEYVNCRTGMPCHLHPSSALYGLGYTPDYVVYHELILTTKEYMQCATSV 1138
Query: 311 DARWLAELGPMFFSVKETGNRIETSVKKQR 222
+ WLAELGPMFFSVK++ + KKQ+
Sbjct: 1139 EPHWLAELGPMFFSVKDSDTSMLEHKKKQK 1168
>At3g26560.1 68416.m03315 ATP-dependent RNA helicase, putative similar
to SP|Q14562 ATP-dependent helicase DDX8 (RNA helicase
HRH1) (DEAH-box protein 8) {Homo sapiens}; contains Pfam
profiles PF04408: Helicase associated domain (HA2),
PF00271: Helicase conserved C-terminal domain, PF00575:
S1 RNA binding domain
Length = 1168
Score = 70.1 bits (164), Expect = 8e-13
Identities = 33/77 (42%), Positives = 44/77 (57%)
Frame = -3
Query: 491 YFQQAARLKGIGEYVNCRTGMPCHLHPTSALFGLGCSPDYVVYHELTMTAREYMHCVTAV 312
+F AR Y P ++HP+SALF PD+V+YH+L MT +EYM VT +
Sbjct: 1061 FFFHGARKDPQEGYRTLVENQPVYIHPSSALFQR--QPDWVIYHDLVMTTKEYMREVTVI 1118
Query: 311 DARWLAELGPMFFSVKE 261
D +WL EL P FF V +
Sbjct: 1119 DPKWLVELAPRFFKVSD 1135
>At1g32490.1 68414.m04009 RNA helicase, putative similar to
ATP-dependent RNA helicase #3 [Homo sapiens] GI:3107913;
contains Pfam profiles PF04408: Helicase associated
domain (HA2), PF00271: Helicase conserved C-terminal
domain
Length = 1044
Score = 63.3 bits (147), Expect = 9e-11
Identities = 30/88 (34%), Positives = 47/88 (53%)
Frame = -3
Query: 491 YFQQAARLKGIGEYVNCRTGMPCHLHPTSALFGLGCSPDYVVYHELTMTAREYMHCVTAV 312
+F A+L+ G Y + H+HP S L + P +VVYHEL +T++EYM VT +
Sbjct: 952 FFPHTAKLQKNGSYRTVKHPQTVHIHPNSGLSQV--LPRWVVYHELVLTSKEYMRQVTEL 1009
Query: 311 DARWLAELGPMFFSVKETGNRIETSVKK 228
WL EL P ++ +K+ + + K
Sbjct: 1010 KPEWLIELAPHYYQLKDVEDAASKKMPK 1037
>At4g16680.1 68417.m02519 RNA helicase, putative similar to
SP|Q14562 ATP-dependent helicase DDX8 (RNA helicase
HRH1) (DEAH-box protein 8) {Homo sapiens}; contains Pfam
profiles PF04408: Helicase associated domain (HA2),
PF00271: Helicase conserved C-terminal domain
Length = 883
Score = 62.1 bits (144), Expect = 2e-10
Identities = 27/77 (35%), Positives = 44/77 (57%)
Frame = -3
Query: 491 YFQQAARLKGIGEYVNCRTGMPCHLHPTSALFGLGCSPDYVVYHELTMTAREYMHCVTAV 312
+F +A+L+ G Y + ++HP S LFG S ++VYHEL +T +EYM T +
Sbjct: 768 FFPHSAKLQKNGSYRRVKEPQTVYVHPNSGLFGASPSK-WLVYHELVLTTKEYMRHTTEM 826
Query: 311 DARWLAELGPMFFSVKE 261
WL E+ P ++ +K+
Sbjct: 827 KPEWLIEIAPHYYKLKD 843
>At2g35340.1 68415.m04333 RNA helicase, putative similar to
ATP-dependent RNA helicase #3 [Homo sapiens] GI:3107913;
contains Pfam profiles PF04408: Helicase associated
domain (HA2), PF00271: Helicase conserved C-terminal
domain
Length = 1110
Score = 62.1 bits (144), Expect = 2e-10
Identities = 31/95 (32%), Positives = 52/95 (54%)
Frame = -3
Query: 491 YFQQAARLKGIGEYVNCRTGMPCHLHPTSALFGLGCSPDYVVYHELTMTAREYMHCVTAV 312
+F A+L+ G Y + H+HP S L + P +VVYH+L +T++EYM VT +
Sbjct: 1018 FFPHTAKLQKNGSYRTVKHPQTVHIHPASGLSQV--LPRWVVYHQLVLTSKEYMRQVTEL 1075
Query: 311 DARWLAELGPMFFSVKETGNRIETSVKKQRSTSNA 207
WL E+ P ++ +K+ + TS K +++ A
Sbjct: 1076 KPEWLIEIAPHYYQLKDVED--ATSKKMPKTSGRA 1108
>At1g26370.1 68414.m03217 RNA helicase, putative similar to
SP|Q14562 ATP-dependent helicase DDX8 (RNA helicase
HRH1) (DEAH-box protein 8) {Homo sapiens}; contains Pfam
profiles PF04408: Helicase associated domain (HA2),
PF00271: Helicase conserved C-terminal domain
Length = 717
Score = 59.3 bits (137), Expect = 1e-09
Identities = 28/77 (36%), Positives = 46/77 (59%)
Frame = -3
Query: 491 YFQQAARLKGIGEYVNCRTGMPCHLHPTSALFGLGCSPDYVVYHELTMTAREYMHCVTAV 312
+F +AA+ + G Y +G H+HPTS LF P+ V+++EL T+++Y+ +T +
Sbjct: 643 FFLKAAQRQLDGTYRALESGEVVHIHPTSVLF--RAKPECVIFNELMQTSKKYIKNLTII 700
Query: 311 DARWLAELGPMFFSVKE 261
D+ WL+EL P F E
Sbjct: 701 DSLWLSELAPHHFQTAE 717
>At3g62310.1 68416.m07000 RNA helicase, putative similar to
SP|P53131 Pre-mRNA splicing factor RNA helicase PRP43
(Helicase JA1) {Saccharomyces cerevisiae}; contains Pfam
profiles PF04408: Helicase associated domain (HA2),
PF00271: Helicase conserved C-terminal domain
Length = 726
Score = 55.2 bits (127), Expect = 2e-08
Identities = 23/80 (28%), Positives = 42/80 (52%)
Frame = -3
Query: 491 YFQQAARLKGIGEYVNCRTGMPCHLHPTSALFGLGCSPDYVVYHELTMTAREYMHCVTAV 312
YF Q A L+ G Y+ + HLHP++ L P++V+Y+E +T+R ++ VT +
Sbjct: 624 YFMQVAHLERTGHYLTVKDNQVVHLHPSNCL---DHKPEWVIYNEYVLTSRNFIRTVTDI 680
Query: 311 DARWLAELGPMFFSVKETGN 252
WL ++ ++ + N
Sbjct: 681 RGEWLVDVASHYYDLSNFPN 700
>At2g47250.1 68415.m05900 RNA helicase, putative similar to
SP|P53131 Pre-mRNA splicing factor RNA helicase PRP43
(Helicase JA1) {Saccharomyces cerevisiae}; contains Pfam
profiles PF04408: Helicase associated domain (HA2),
PF00271: Helicase conserved C-terminal domain
Length = 729
Score = 54.8 bits (126), Expect = 3e-08
Identities = 23/80 (28%), Positives = 41/80 (51%)
Frame = -3
Query: 491 YFQQAARLKGIGEYVNCRTGMPCHLHPTSALFGLGCSPDYVVYHELTMTAREYMHCVTAV 312
YF Q A L+ G Y+ + HLHP++ L P++V+Y+E +T R ++ VT +
Sbjct: 628 YFMQVAHLERTGHYLTVKDNQVVHLHPSNCL---DHKPEWVIYNEYVLTTRNFIRTVTDI 684
Query: 311 DARWLAELGPMFFSVKETGN 252
WL ++ ++ + N
Sbjct: 685 RGEWLVDVAQHYYDLSNFPN 704
>At5g14900.1 68418.m01748 helicase associated (HA2)
domain-containing protein similar to SP|P53131 Pre-mRNA
splicing factor RNA helicase PRP43 (Helicase JA1)
{Saccharomyces cerevisiae}; contains Pfam profile
PF04408: Helicase associated domain (HA2)
Length = 301
Score = 52.4 bits (120), Expect = 2e-07
Identities = 25/77 (32%), Positives = 41/77 (53%), Gaps = 2/77 (2%)
Frame = -3
Query: 491 YFQQAARLKGIGEYVNCRT--GMPCHLHPTSALFGLGCSPDYVVYHELTMTAREYMHCVT 318
YF Q A L+ G Y+ R HLHP++ L P++VVY+E T+R ++ VT
Sbjct: 195 YFMQVAHLERTGHYLTFRDKDDQVVHLHPSNCL---DHKPEWVVYNEYVFTSRNFIRTVT 251
Query: 317 AVDARWLAELGPMFFSV 267
+ WL ++ P ++ +
Sbjct: 252 HIRGEWLVDVAPHYYKL 268
>At4g18465.1 68417.m02740 RNA helicase, putative similar to
SP|Q14562 ATP-dependent helicase DDX8 (RNA helicase
HRH1) (DEAH-box protein 8) {Homo sapiens}; contains Pfam
profiles PF04408: Helicase associated domain (HA2),
PF00271: Helicase conserved C-terminal domain
Length = 704
Score = 52.4 bits (120), Expect = 2e-07
Identities = 26/79 (32%), Positives = 42/79 (53%), Gaps = 2/79 (2%)
Frame = -3
Query: 491 YFQQAARLK--GIGEYVNCRTGMPCHLHPTSALFGLGCSPDYVVYHELTMTAREYMHCVT 318
+F A RL+ G Y R ++HP+S LF + +P +VVY + T R+YM V
Sbjct: 623 FFANACRLEPHSNGVYKTIRGSEEVYIHPSSVLFRV--NPKWVVYQSIVSTERQYMRNVV 680
Query: 317 AVDARWLAELGPMFFSVKE 261
++ WL E+ P F+ ++
Sbjct: 681 TINPSWLTEVAPHFYQNRQ 699
>At1g27900.1 68414.m03419 RNA helicase, putative similar to
SP|Q14562 ATP-dependent helicase DDX8 (RNA helicase
HRH1) (DEAH-box protein 8) {Homo sapiens}; contains Pfam
profiles PF04408: Helicase associated domain (HA2),
PF00271: Helicase conserved C-terminal domain
Length = 700
Score = 44.8 bits (101), Expect = 3e-05
Identities = 22/46 (47%), Positives = 28/46 (60%), Gaps = 2/46 (4%)
Frame = -3
Query: 419 LHPTSALFGL--GCSPDYVVYHELTMTAREYMHCVTAVDARWLAEL 288
+HP+S L G P+YVVYHEL T R +M V AVD W+A +
Sbjct: 594 VHPSSVLSADNDGMMPNYVVYHELISTTRPFMRNVCAVDMAWVAPI 639
>At1g33390.1 68414.m04133 helicase domain-containing protein similar
to kurz protein [Drosophila melanogaster] GI:5869803;
contains Pfam profiles PF04408: Helicase associated
domain (HA2), PF00271: Helicase conserved C-terminal
domain
Length = 1237
Score = 44.0 bits (99), Expect = 6e-05
Identities = 24/64 (37%), Positives = 32/64 (50%)
Frame = -3
Query: 482 QAARLKGIGEYVNCRTGMPCHLHPTSALFGLGCSPDYVVYHELTMTAREYMHCVTAVDAR 303
+ AR EY C P LH S+L + +P+ +VY EL +T R YMH T V
Sbjct: 1019 RVARKTRATEYQACAVQEPVFLHRWSSL--INSAPELLVYSELLLTNRPYMHGATRVRPE 1076
Query: 302 WLAE 291
WL +
Sbjct: 1077 WLVK 1080
>At5g10370.1 68418.m01203 helicase domain-containing protein / IBR
domain-containing protein / zinc finger protein-related
similar to RNA-dependent ATPase/helicase Cdc28p
[Schizosaccharomyces pombe] GI:1439562; contains Pfam
profiles PF04408: Helicase associated domain (HA2),
PF00271: Helicase conserved C-terminal domain, weak hit
to PF00097: Zinc finger, C3HC4 type (RING finger),
PF01485: IBR domain
Length = 1775
Score = 43.6 bits (98), Expect = 8e-05
Identities = 21/52 (40%), Positives = 27/52 (51%)
Frame = -3
Query: 437 TGMPCHLHPTSALFGLGCSPDYVVYHELTMTAREYMHCVTAVDARWLAELGP 282
TG LHP+ +L G P +VV+ EL +Y+ CVTA D L L P
Sbjct: 888 TGQQVQLHPSCSLLAFGQKPSWVVFGELLSIVDQYLVCVTACDFEALYMLDP 939
>At4g01020.1 68417.m00137 helicase domain-containing protein / IBR
domain-containing protein / zinc finger protein-related
similar to SP|Q14562 ATP-dependent helicase DDX8 (RNA
helicase HRH1) (DEAH-box protein 8) {Homo sapiens};
contains Pfam profiles PF04408: Helicase associated
domain (HA2), PF00271: Helicase conserved C-terminal
domain, PF00097: Zinc finger, C3HC4 type (RING finger),
PF01485: IBR domain
Length = 1787
Score = 41.1 bits (92), Expect = 4e-04
Identities = 20/52 (38%), Positives = 26/52 (50%)
Frame = -3
Query: 437 TGMPCHLHPTSALFGLGCSPDYVVYHELTMTAREYMHCVTAVDARWLAELGP 282
T LHP+ +L G P +VV+ EL +Y+ CVTA D L L P
Sbjct: 885 TSQQVQLHPSCSLLAFGQKPSWVVFGELLSIVDQYLVCVTAFDFEALYMLDP 936
>At1g78700.1 68414.m09173 brassinosteroid signalling positive
regulator-related contains similarity to BZR1 protein
[Arabidopsis thaliana] gi|20270971|gb|AAM18490
Length = 325
Score = 29.1 bits (62), Expect = 1.8
Identities = 17/47 (36%), Positives = 19/47 (40%), Gaps = 1/47 (2%)
Frame = +2
Query: 362 GTPRSRGCS-PARTAPRWGAGGTACRCGSSRTRRCPSAAPPAGSKVF 499
GT +GCS P G TA C S + C S P GS F
Sbjct: 67 GTTYRKGCSRPVERMEIGGGSATASPCSSYQPSPCASYNPSPGSSNF 113
>At5g47210.1 68418.m05821 nuclear RNA-binding protein, putative
similar to nuclear RNA binding protein GI:6492264 from
[Arabidopsis thaliana]
Length = 357
Score = 28.3 bits (60), Expect = 3.1
Identities = 14/30 (46%), Positives = 17/30 (56%)
Frame = +2
Query: 368 PRSRGCSPARTAPRWGAGGTACRCGSSRTR 457
P S+ +R AP+ G GGT R G SR R
Sbjct: 49 PPSQAVRESRNAPQGGRGGTGGRGGFSRGR 78
>At5g18410.2 68418.m02167 expressed protein similar to p53 inducible
protein [Homo sapiens] GI:5616320
Length = 1017
Score = 27.9 bits (59), Expect = 4.1
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = -2
Query: 456 RVRELPHRHAVPPAPHLGAVRA 391
R+ LP H +PP H+GA+RA
Sbjct: 342 RLLTLPAPHELPPLNHIGALRA 363
>At5g18410.1 68418.m02166 expressed protein similar to p53 inducible
protein [Homo sapiens] GI:5616320
Length = 1234
Score = 27.9 bits (59), Expect = 4.1
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = -2
Query: 456 RVRELPHRHAVPPAPHLGAVRA 391
R+ LP H +PP H+GA+RA
Sbjct: 342 RLLTLPAPHELPPLNHIGALRA 363
>At3g24650.1 68416.m03095 abscisic acid-insensitive protein 3 (ABI3)
identical to abscisic acid-insensitive protein 3
GI:16146 SP:Q01593 from [Arabidopsis thaliana], (Plant
Cell 4 (10), 1251-1261 (1992))
Length = 720
Score = 27.5 bits (58), Expect = 5.4
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = +3
Query: 312 HGGDAVHVLARRHGELVVHH 371
HGGD HV + +L+VHH
Sbjct: 45 HGGDNNHVHGHQDDDLIVHH 64
>At2g33360.1 68415.m04089 expressed protein
Length = 603
Score = 26.6 bits (56), Expect = 9.4
Identities = 14/35 (40%), Positives = 17/35 (48%)
Frame = +2
Query: 362 GTPRSRGCSPARTAPRWGAGGTACRCGSSRTRRCP 466
G PR+R P+ RW +GG C C S CP
Sbjct: 488 GGPRNRNGGPSSLIQRWKSGG-CCDC-SGWDLGCP 520
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,190,337
Number of Sequences: 28952
Number of extensions: 139308
Number of successful extensions: 481
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 464
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 473
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 888318720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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