Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30232X
(498 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g11980.1 68416.m01482 male sterility protein 2 (MS2) identica... 55 2e-08
At3g56700.1 68416.m06307 male sterility protein, putative simila... 55 3e-08
At5g22500.1 68418.m02626 acyl CoA reductase, putative / male-ste... 44 4e-05
At3g44560.1 68416.m04789 acyl CoA reductase, putative similar to... 42 2e-04
At3g44550.1 68416.m04788 acyl CoA reductase, putative similar to... 42 2e-04
At3g60060.1 68416.m06707 hypothetical protein male sterility pro... 40 7e-04
At5g22420.1 68418.m02615 acyl CoA reductase, putative similar to... 39 0.002
At3g44540.1 68416.m04787 acyl CoA reductase, putative similar to... 37 0.009
At1g78570.1 68414.m09157 NAD-dependent epimerase/dehydratase fam... 31 0.33
At3g14790.1 68416.m01869 NAD-dependent epimerase/dehydratase fam... 31 0.57
At5g07790.1 68418.m00892 expressed protein 30 0.75
At1g53500.1 68414.m06066 NAD-dependent epimerase/dehydratase fam... 30 0.75
At2g28760.2 68415.m03498 NAD-dependent epimerase/dehydratase fam... 29 1.3
At2g28760.1 68415.m03497 NAD-dependent epimerase/dehydratase fam... 29 1.3
At2g33630.1 68415.m04123 3-beta hydroxysteroid dehydrogenase/iso... 29 1.7
At3g16940.1 68416.m02165 calmodulin-binding protein similar to a... 28 4.0
At5g56250.2 68418.m07020 expressed protein 27 5.3
At5g56250.1 68418.m07019 expressed protein 27 5.3
At3g12060.1 68416.m01500 expressed protein similar to hypothetic... 27 5.3
At5g40170.1 68418.m04875 disease resistance family protein conta... 27 7.0
At5g55540.1 68418.m06919 expressed protein 27 9.3
At5g39450.1 68418.m04778 F-box family protein contains Pfam:PF00... 27 9.3
At3g57480.1 68416.m06399 zinc finger (C2H2 type, AN1-like) famil... 27 9.3
At3g50380.1 68416.m05511 expressed protein 27 9.3
>At3g11980.1 68416.m01482 male sterility protein 2 (MS2) identical
to male sterility protein 2 (MS2) SP:Q08891 (Arabidopsis
thaliana)
Length = 616
Score = 55.2 bits (127), Expect = 2e-08
Identities = 26/50 (52%), Positives = 33/50 (66%)
Frame = +1
Query: 88 MTEESLVRAFYSGKNFFITGGTGFVGLCLIEKILRGIPDVGKVYLLMRPK 237
M E + +F GK F ITG TGF+ LIEK+LR PDV K+YLL++ K
Sbjct: 118 MKEGLGIISFLQGKKFLITGSTGFLAKVLIEKVLRMAPDVSKIYLLIKAK 167
Score = 40.3 bits (90), Expect = 7e-04
Identities = 18/54 (33%), Positives = 32/54 (59%)
Frame = +3
Query: 330 KLVPISGDVGEANLGLSPQDRQLLIENVNVVIHSAATLDFQENLRPTDRINPLG 491
KL+P++G++ ++N+GL + + + V+V+I+SAA F E IN G
Sbjct: 204 KLIPVTGNICDSNIGLQADSAEEIAKEVDVIINSAANTTFNERYDVALDINTRG 257
>At3g56700.1 68416.m06307 male sterility protein, putative similar
to SP|Q08891 Male sterility protein 2 {Arabidopsis
thaliana}; contains Pfam profile PF03015: Male sterility
protein
Length = 527
Score = 54.8 bits (126), Expect = 3e-08
Identities = 29/79 (36%), Positives = 43/79 (54%), Gaps = 1/79 (1%)
Frame = +1
Query: 4 RRNNKSCFIIIIVNHSYISQDTPEGRNKMTEESL-VRAFYSGKNFFITGGTGFVGLCLIE 180
RR SCF + S TP+ + + + F GK++ +TG TGF+ LIE
Sbjct: 42 RRVQTSCFYGETSFEAVTSLVTPKTETSRNSDGIGIVRFLEGKSYLVTGATGFLAKVLIE 101
Query: 181 KILRGIPDVGKVYLLMRPK 237
K+LR ++GK++LLMR K
Sbjct: 102 KLLRESLEIGKIFLLMRSK 120
Score = 39.1 bits (87), Expect = 0.002
Identities = 16/55 (29%), Positives = 29/55 (52%)
Frame = +3
Query: 327 KKLVPISGDVGEANLGLSPQDRQLLIENVNVVIHSAATLDFQENLRPTDRINPLG 491
+KL+P+ GD+ E NLG+ + ++ E ++V+I F + +N LG
Sbjct: 156 RKLIPVIGDIEEDNLGIKSEIANMISEEIDVIISCGGRTTFDDRYDSALSVNALG 210
>At5g22500.1 68418.m02626 acyl CoA reductase, putative /
male-sterility protein, putative similar to acyl CoA
reductase [Simmondsia chinensis] GI:5020215; contains
Pfam profile PF03015: Male sterility protein; identical
to cDNA male sterility 2-like protein GI:1491614
Length = 491
Score = 44.4 bits (100), Expect = 4e-05
Identities = 25/60 (41%), Positives = 31/60 (51%)
Frame = +1
Query: 97 ESLVRAFYSGKNFFITGGTGFVGLCLIEKILRGIPDVGKVYLLMRPKKGKEISND*RSSL 276
ES F K ITG GF+ L+EKILR P+V K+YLL+R K RS +
Sbjct: 2 ESNCVQFLGNKTILITGAPGFLAKVLVEKILRLQPNVKKIYLLLRAPDEKSAMQRLRSEV 61
Score = 37.1 bits (82), Expect = 0.007
Identities = 20/56 (35%), Positives = 31/56 (55%), Gaps = 1/56 (1%)
Frame = +3
Query: 327 KKLVPISGDVGEANLGLSPQDR-QLLIENVNVVIHSAATLDFQENLRPTDRINPLG 491
+K+VP+ GD+ NLGL D Q + ++++I+ AAT +F E IN G
Sbjct: 85 EKIVPVPGDISIDNLGLKDTDLIQRMWSEIDIIINIAATTNFDERYDIGLGINTFG 140
>At3g44560.1 68416.m04789 acyl CoA reductase, putative similar to
acyl CoA reductase [Simmondsia chinensis] GI:5020215;
contains Pfam profile PF03015: Male sterility protein
Length = 449
Score = 42.3 bits (95), Expect = 2e-04
Identities = 18/39 (46%), Positives = 25/39 (64%)
Frame = +1
Query: 115 FYSGKNFFITGGTGFVGLCLIEKILRGIPDVGKVYLLMR 231
F K +TG TGF+ +EKILR P+V K+YL++R
Sbjct: 8 FLQNKTILVTGATGFLAKVFVEKILRVQPNVNKLYLVVR 46
Score = 37.1 bits (82), Expect = 0.007
Identities = 26/85 (30%), Positives = 46/85 (54%), Gaps = 7/85 (8%)
Frame = +3
Query: 258 RLEEFPKNLVFEKLLDTNTTD------IFKKLVPISGDVGEANLGLSPQD-RQLLIENVN 416
R E F K+L K+L N D + +K+VP++GD+ +LG+ + R+ + + ++
Sbjct: 58 RTEAFEKDLF--KVLRDNLGDEKLNTLLSEKVVPVAGDIAMDHLGMKDSNLRERMQKEID 115
Query: 417 VVIHSAATLDFQENLRPTDRINPLG 491
+V++ AAT +F E IN G
Sbjct: 116 IVVNVAATTNFDERYDIGLGINTFG 140
>At3g44550.1 68416.m04788 acyl CoA reductase, putative similar to
acyl CoA reductase [Simmondsia chinensis] GI:5020215;
contains Pfam profile PF03015: Male sterility protein
Length = 402
Score = 42.3 bits (95), Expect = 2e-04
Identities = 19/39 (48%), Positives = 25/39 (64%)
Frame = +1
Query: 115 FYSGKNFFITGGTGFVGLCLIEKILRGIPDVGKVYLLMR 231
F K +TG TGF+ +EKILR P+V K+YLL+R
Sbjct: 8 FLRNKTILVTGATGFLAKVFVEKILRVQPNVKKLYLLVR 46
Score = 34.7 bits (76), Expect = 0.035
Identities = 22/73 (30%), Positives = 40/73 (54%), Gaps = 1/73 (1%)
Frame = +3
Query: 276 KNLVFEKLLDTNTTDIFKKLVPISGDVGEANLGLSPQD-RQLLIENVNVVIHSAATLDFQ 452
+NL EKL T +++K+V + GD+ LG++ R+ + + +++V++ AAT +F
Sbjct: 72 QNLGDEKL----NTLLYEKVVSVPGDIATDQLGINDSHLRERMQKEIDIVVNVAATTNFD 127
Query: 453 ENLRPTDRINPLG 491
E IN G
Sbjct: 128 ERYDVGLGINTFG 140
>At3g60060.1 68416.m06707 hypothetical protein male sterility
protein 2 - Brassica napus, PIR:T08096
Length = 154
Score = 40.3 bits (90), Expect = 7e-04
Identities = 17/47 (36%), Positives = 29/47 (61%)
Frame = +3
Query: 321 IFKKLVPISGDVGEANLGLSPQDRQLLIENVNVVIHSAATLDFQENL 461
I KL+P+ D+ E NLG+ + + E ++++I SAAT F ++L
Sbjct: 52 ILSKLLPVVEDIAEDNLGVDSETSLKISEEIDIIISSAATTTFDDSL 98
>At5g22420.1 68418.m02615 acyl CoA reductase, putative similar to
acyl CoA reductase [Simmondsia chinensis] GI:5020215;
contains Pfam profile PF03015: Male sterility protein
Length = 409
Score = 39.1 bits (87), Expect = 0.002
Identities = 17/44 (38%), Positives = 27/44 (61%)
Frame = +1
Query: 115 FYSGKNFFITGGTGFVGLCLIEKILRGIPDVGKVYLLMRPKKGK 246
F + +TG +GF+ L+E+ILR P+V ++YLL+R K
Sbjct: 8 FLENRTILVTGASGFLAKVLVERILRLQPNVKRLYLLVRASDKK 51
>At3g44540.1 68416.m04787 acyl CoA reductase, putative similar to
acyl CoA reductase [Simmondsia chinensis] GI:5020215;
contains Pfam profile PF03015: Male sterility protein
Length = 433
Score = 36.7 bits (81), Expect = 0.009
Identities = 19/58 (32%), Positives = 32/58 (55%), Gaps = 1/58 (1%)
Frame = +3
Query: 321 IFKKLVPISGDVGEANLGLSPQD-RQLLIENVNVVIHSAATLDFQENLRPTDRINPLG 491
I +K+VPI GD+ NLG+ D Q + ++++++ AAT +F E +N G
Sbjct: 83 ITEKVVPIPGDISVDNLGVKGSDLLQHMWNEIDIIVNVAATTNFDERYDVGLSVNTFG 140
Score = 35.9 bits (79), Expect = 0.015
Identities = 17/39 (43%), Positives = 23/39 (58%)
Frame = +1
Query: 115 FYSGKNFFITGGTGFVGLCLIEKILRGIPDVGKVYLLMR 231
F K +TG GF+ +EKILR P V K++LL+R
Sbjct: 8 FLHDKTILVTGVPGFLAKVFVEKILRIQPKVKKLFLLLR 46
>At1g78570.1 68414.m09157 NAD-dependent epimerase/dehydratase family
protein similar to dTDP-glucose 4,6-dehydratase from
Aneurinibacillus thermoaerophilus GI:16357461, RmlB from
Leptospira borgpetersenii GI:4234803; contains Pfam
profile PF01370 NAD dependent epimerase/dehydratase
family
Length = 669
Score = 31.5 bits (68), Expect = 0.33
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = +1
Query: 112 AFYSGKNFFITGGTGFVGLCLIEKILRGIPDVGKVYL 222
A Y+ KN ITG GF+ + +++R PD V L
Sbjct: 2 ASYTPKNILITGAAGFIASHVANRLIRSYPDYKIVVL 38
>At3g14790.1 68416.m01869 NAD-dependent epimerase/dehydratase family
protein similar to dTDP-glucose 4,6-dehydratase from
Aneurinibacillus thermoaerophilus GI:16357461,
Saccharopolyspora spinosa GI:15077647, RmlB from
Leptospira borgpetersenii GI:4234803; contains Pfam
profile PF01370 NAD dependent epimerase/dehydratase
family
Length = 664
Score = 30.7 bits (66), Expect = 0.57
Identities = 14/37 (37%), Positives = 19/37 (51%)
Frame = +1
Query: 112 AFYSGKNFFITGGTGFVGLCLIEKILRGIPDVGKVYL 222
A Y KN ITG GF+ + +++R PD V L
Sbjct: 2 ATYKPKNILITGAAGFIASHVANRLVRSYPDYKIVVL 38
>At5g07790.1 68418.m00892 expressed protein
Length = 616
Score = 30.3 bits (65), Expect = 0.75
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = +2
Query: 323 LQEAGPDLWRCW*GESRSKSSRPAAVD 403
L EA W CW +++SKSS P VD
Sbjct: 550 LMEASDLKWNCWFPKNKSKSSPPKPVD 576
>At1g53500.1 68414.m06066 NAD-dependent epimerase/dehydratase family
protein low similarity to dTDP-D-glucose-4,6-dehydratase
from Aneurinibacillus thermoaerophilus GI:16357461,
Sphingomonas sp. GI:1314581; contains Pfam profile
PF01370: NAD dependent epimerase/dehydratase family;
putative NDP-rhamnose synthase (rhm2 gene) GI:31559258
Length = 667
Score = 30.3 bits (65), Expect = 0.75
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = +1
Query: 118 YSGKNFFITGGTGFVGLCLIEKILRGIPDVGKVYL 222
Y KN ITG GF+ + +++R PD V L
Sbjct: 6 YKPKNILITGAAGFIASHVANRLIRNYPDYKIVVL 40
>At2g28760.2 68415.m03498 NAD-dependent epimerase/dehydratase family
protein similar to UDP-glucuronic acid decarboxylase
Uxs1p from Filobasidiella neoformans GI:14318327;
contains Pfam profile PF01370 NAD dependent
epimerase/dehydratase family
Length = 343
Score = 29.5 bits (63), Expect = 1.3
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = +1
Query: 115 FYSGKNFFITGGTGFVGLCLIEKILR 192
F S +TGG GF+G L++K+++
Sbjct: 27 FQSNMRILVTGGAGFIGSHLVDKLMQ 52
>At2g28760.1 68415.m03497 NAD-dependent epimerase/dehydratase family
protein similar to UDP-glucuronic acid decarboxylase
Uxs1p from Filobasidiella neoformans GI:14318327;
contains Pfam profile PF01370 NAD dependent
epimerase/dehydratase family
Length = 343
Score = 29.5 bits (63), Expect = 1.3
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = +1
Query: 115 FYSGKNFFITGGTGFVGLCLIEKILR 192
F S +TGG GF+G L++K+++
Sbjct: 27 FQSNMRILVTGGAGFIGSHLVDKLMQ 52
>At2g33630.1 68415.m04123 3-beta hydroxysteroid
dehydrogenase/isomerase family protein contains Pfam
profile PF01073 3-beta hydroxysteroid
dehydrogenase/isomerase domain; similar to
NAD(P)-dependent cholesterol dehydrogenase [Nocardia
sp.] [GI:216809]
Length = 480
Score = 29.1 bits (62), Expect = 1.7
Identities = 14/29 (48%), Positives = 17/29 (58%), Gaps = 1/29 (3%)
Frame = +1
Query: 124 GKNFFITGGTGFVGLCL-IEKILRGIPDV 207
G F +TGG GFVG L +E + RG V
Sbjct: 11 GNTFVVTGGLGFVGAALCLELVRRGARQV 39
>At3g16940.1 68416.m02165 calmodulin-binding protein similar to
anther ethylene-upregulated protein ER1 GI:11612392 from
[Nicotiana tabacum]; contains Pfam profile: PF00612 IQ
calmodulin-binding motif (3 copies)
Length = 852
Score = 27.9 bits (59), Expect = 4.0
Identities = 12/36 (33%), Positives = 21/36 (58%)
Frame = +3
Query: 375 LSPQDRQLLIENVNVVIHSAATLDFQENLRPTDRIN 482
++ +D ++ N ++ +H TLD+ E L PTD N
Sbjct: 171 VAAEDIDTVVRNHDISLHDINTLDWDELLVPTDLNN 206
>At5g56250.2 68418.m07020 expressed protein
Length = 811
Score = 27.5 bits (58), Expect = 5.3
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = +1
Query: 1 DRRNNKSCFIIIIVNHSYISQDTPEGRNKMTEESLVRA 114
D+RN +I ++ +S D PE NK+ + S+ RA
Sbjct: 242 DKRNGTKWAMISSYSNRVVSDDKPEVSNKVKKRSVSRA 279
>At5g56250.1 68418.m07019 expressed protein
Length = 811
Score = 27.5 bits (58), Expect = 5.3
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = +1
Query: 1 DRRNNKSCFIIIIVNHSYISQDTPEGRNKMTEESLVRA 114
D+RN +I ++ +S D PE NK+ + S+ RA
Sbjct: 242 DKRNGTKWAMISSYSNRVVSDDKPEVSNKVKKRSVSRA 279
>At3g12060.1 68416.m01500 expressed protein similar to hypothetical
protein GB:CAB82953 GI:7340710 from [Arabidopsis
thaliana]
Length = 556
Score = 27.5 bits (58), Expect = 5.3
Identities = 15/47 (31%), Positives = 20/47 (42%)
Frame = +3
Query: 255 QRLEEFPKNLVFEKLLDTNTTDIFKKLVPISGDVGEANLGLSPQDRQ 395
+ L KNL FE + TTD K + I G A +S D +
Sbjct: 103 ENLTAVTKNLTFESPVANGTTDTNAKTITIQFQTGHAKENISCPDNK 149
>At5g40170.1 68418.m04875 disease resistance family protein contains
leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; similar to Cf-4 [Lycopersicon
hirsutum] gi|2808683|emb|CAA05268
Length = 792
Score = 27.1 bits (57), Expect = 7.0
Identities = 13/40 (32%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
Frame = -3
Query: 370 RFASP-TSPEIGTSFLKISVVFVSRSFSNTRFLGNSSSRW 254
RF P +SPE+ SF + ++ +SR+ N N + W
Sbjct: 525 RFHGPISSPEVSLSFTALRIIDISRNSFNGSLPQNYFANW 564
>At5g55540.1 68418.m06919 expressed protein
Length = 1380
Score = 26.6 bits (56), Expect = 9.3
Identities = 18/49 (36%), Positives = 21/49 (42%)
Frame = +1
Query: 88 MTEESLVRAFYSGKNFFITGGTGFVGLCLIEKILRGIPDVGKVYLLMRP 234
+TE VRAF G+N+ G T L G P V V LM P
Sbjct: 494 LTEPKSVRAFLCGQNY--AGKTTLCNSILQSSSASGFPYVENVRNLMNP 540
>At5g39450.1 68418.m04778 F-box family protein contains Pfam:PF00646
F-box domain ; similar to SKP1 interacting partner 2
(SKIP2) TIGR_Ath1:At5g67250
Length = 579
Score = 26.6 bits (56), Expect = 9.3
Identities = 16/54 (29%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
Frame = +1
Query: 103 LVRAFYSGKNFFITGGTGFVGLCLIEKILRGIPDVGKVYLL-MRPKKGKEISND 261
++ F FF+ G G V CL +RGI V LL + ++ K++ N+
Sbjct: 150 IICGFDGSAKFFLHGRDGKVQCCLHPGFVRGIEKSCNVLLLEVETRREKKLCNE 203
>At3g57480.1 68416.m06399 zinc finger (C2H2 type, AN1-like) family
protein contains Pfam domain, PF00096: Zinc finger, C2H2
type; contains Pfam domain, PF01428: AN1-like Zinc
finger
Length = 249
Score = 26.6 bits (56), Expect = 9.3
Identities = 18/51 (35%), Positives = 25/51 (49%)
Frame = -3
Query: 364 ASPTSPEIGTSFLKISVVFVSRSFSNTRFLGNSSSRWIFLFLS*VSLISKL 212
+ P PE SF+ F+S + +SSSRW LF S + IS+L
Sbjct: 138 SGPKKPESSFSFMG----FLSTNTKEAPASSSSSSRWSSLFASAEASISRL 184
>At3g50380.1 68416.m05511 expressed protein
Length = 3071
Score = 26.6 bits (56), Expect = 9.3
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +1
Query: 85 KMTEESLVRAFYSGKNFFITGGTGFVGLCLIEKIL 189
KM E+SL+ F K F+T + +C IEK L
Sbjct: 343 KMPEKSLLWKFSENKRHFVTARRKWEMICNIEKEL 377
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,253,488
Number of Sequences: 28952
Number of extensions: 203089
Number of successful extensions: 478
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 468
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 477
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 878448512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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