Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30226
(747 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g21326.1 68414.m02666 VQ motif-containing protein contains PF... 31 1.1
At5g15050.1 68418.m01764 glycosyltransferase family 14 protein /... 29 2.5
At1g21320.1 68414.m02664 VQ motif-containing protein contains PF... 29 2.5
At4g30870.1 68417.m04383 repair endonuclease family protein cont... 29 4.3
At2g41560.1 68415.m05136 calcium-transporting ATPase 4, plasma m... 29 4.3
At1g69830.1 68414.m08034 alpha-amylase, putative / 1,4-alpha-D-g... 29 4.3
At2g39930.1 68415.m04907 isoamylase, putative / starch debranchi... 28 5.7
At5g02110.1 68418.m00132 cyclin family protein low similarity to... 28 7.6
At4g26090.1 68417.m03756 disease resistance protein RPS2 (CC-NBS... 28 7.6
At3g51520.1 68416.m05642 diacylglycerol acyltransferase family c... 28 7.6
At2g26420.1 68415.m03170 1-phosphatidylinositol-4-phosphate 5-ki... 28 7.6
At1g74070.1 68414.m08579 peptidyl-prolyl cis-trans isomerase cyc... 28 7.6
At1g09980.1 68414.m01126 expressed protein contains Pfam profile... 28 7.6
At5g59130.1 68418.m07411 subtilase family protein contains simil... 27 10.0
At2g20590.2 68415.m02408 reticulon family protein non-consensus ... 27 10.0
At2g20590.1 68415.m02407 reticulon family protein non-consensus ... 27 10.0
>At1g21326.1 68414.m02666 VQ motif-containing protein contains
PF05678: VQ motif
Length = 239
Score = 30.7 bits (66), Expect = 1.1
Identities = 22/75 (29%), Positives = 33/75 (44%), Gaps = 9/75 (12%)
Frame = -2
Query: 356 HAQPGHPEQHEQRPTQVHPER----IVSILQYLLSRHPVNC-----RLLASSSIFASPFL 204
H QP P+ H+Q P+Q P I ++ ++ HP N RL +S +
Sbjct: 38 HPQPQPPQTHQQEPSQSRPPPGPVIIYTVSPRIIHTHPNNFMTLVQRLTGKTSTSTTSSS 97
Query: 203 WSPTTYFASDMSTRV 159
+S +T D ST V
Sbjct: 98 YSSSTSAPKDASTMV 112
>At5g15050.1 68418.m01764 glycosyltransferase family 14 protein /
core-2/I-branching enzyme family protein contains Pfam
profile: PF02485 Core-2/I-Branching enzyme
Length = 434
Score = 29.5 bits (63), Expect = 2.5
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = +2
Query: 650 VFTNHTWFVQSENSTEPYTDYFIWTKDNL 736
+FT W + S P+ DYFIW DNL
Sbjct: 261 LFTGSAWMMLSR----PFVDYFIWGWDNL 285
>At1g21320.1 68414.m02664 VQ motif-containing protein contains
PF05678: VQ motif
Length = 235
Score = 29.5 bits (63), Expect = 2.5
Identities = 22/75 (29%), Positives = 32/75 (42%), Gaps = 9/75 (12%)
Frame = -2
Query: 356 HAQPGHPEQHEQRPTQVHPE----RIVSILQYLLSRHPVNC-----RLLASSSIFASPFL 204
H QP P+ H+Q P+Q P I ++ ++ HP N RL +S +
Sbjct: 38 HPQPQPPQTHQQEPSQSRPPPGPVNIYTVTPRIIHTHPNNFMTLVQRLTGQTSTSTTSSS 97
Query: 203 WSPTTYFASDMSTRV 159
S +T D ST V
Sbjct: 98 SSSSTSEPKDTSTMV 112
>At4g30870.1 68417.m04383 repair endonuclease family protein
contains Pfam PF02732 : ERCC4 domain; similar to repair
endonuclease (TIGR:At5g41150) [Arabidopsis thaliana]
Length = 661
Score = 28.7 bits (61), Expect = 4.3
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +3
Query: 504 KTSKFRACSSKCRLTKCSTNVTHWTNSKFCSTKLRR 611
+ KFR+C+ L CS+ +H K CS+ +RR
Sbjct: 302 RAKKFRSCNDGSTLNPCSSGSSHAV--KACSSSVRR 335
>At2g41560.1 68415.m05136 calcium-transporting ATPase 4, plasma
membrane-type / Ca2+-ATPase, isoform 4 (ACA4) identical
to SP|O22218 Calcium-transporting ATPase 4, plasma
membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 4)
{Arabidopsis thaliana}
Length = 1030
Score = 28.7 bits (61), Expect = 4.3
Identities = 8/31 (25%), Positives = 22/31 (70%)
Frame = +1
Query: 421 GTNSVLWSDWSWSMPSNHKIYNRIWNC*RLQ 513
G+++ + +D + ++ +NH + N++W C ++Q
Sbjct: 443 GSSTCICTDKTGTLTTNHMVVNKVWICDKVQ 473
>At1g69830.1 68414.m08034 alpha-amylase, putative /
1,4-alpha-D-glucan glucanohydrolase, putative similar to
SP|P17859 Alpha-amylase precursor (EC 3.2.1.1)
(1,4-alpha-D-glucan glucanohydrolase) {Vigna mungo},
alpha-amylase [Malus x domestica] GI:7532799; contains
Pfam profile PF00128: Alpha amylase, catalytic domain
Length = 887
Score = 28.7 bits (61), Expect = 4.3
Identities = 11/35 (31%), Positives = 20/35 (57%)
Frame = +2
Query: 545 YEVLDKRDTLDEFKILFNKIKKIGVRVIVDLIPNY 649
Y + + T+DE K K K+G++V+ D + N+
Sbjct: 551 YNLNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNH 585
>At2g39930.1 68415.m04907 isoamylase, putative / starch debranching
enzyme, putative similar to isoamylase from [Solanum
tuberosum] GI:27728145, [Triticum aestivum] GI:17932898,
[Hordeum vulgare] GI:21314275, [Oryza sativa]
GI:3252794; contains Pfam profiles PF00128: Alpha
amylase catalytic domain, PF02922: Isoamylase N-terminal
domain
Length = 783
Score = 28.3 bits (60), Expect = 5.7
Identities = 11/26 (42%), Positives = 19/26 (73%)
Frame = +2
Query: 572 LDEFKILFNKIKKIGVRVIVDLIPNY 649
++EFKIL + K G+ VI+D++ N+
Sbjct: 315 INEFKILVKEAHKRGIEVIMDVVLNH 340
>At5g02110.1 68418.m00132 cyclin family protein low similarity to
cyclin D3.1 from [Lycopersicon esculentum] GI:5679622,
[Nicotiana tabacum] GI:4160300; contains Pfam profiles
PF00134: Cyclin, N-terminal domain, PF02984: Cyclin,
C-terminal domain
Length = 341
Score = 27.9 bits (59), Expect = 7.6
Identities = 9/15 (60%), Positives = 13/15 (86%)
Frame = -3
Query: 667 GMVGEHVVRNQINNH 623
GMVG+H++ N+I NH
Sbjct: 194 GMVGDHMIMNRITNH 208
>At4g26090.1 68417.m03756 disease resistance protein RPS2
(CC-NBS-LRR class), putative domain signature CC-NBS-LRR
exists, suggestive of a disease resistance protein.
identical to RPS2 (gi:13661831)
Length = 909
Score = 27.9 bits (59), Expect = 7.6
Identities = 16/46 (34%), Positives = 26/46 (56%), Gaps = 6/46 (13%)
Frame = -3
Query: 688 VLTLYEPGMVGEHVVRNQINN------HSYSDLLNFVEQNFEFVQC 569
++ +Y PG VG+ + INN H Y D+L +V+ + EF +C
Sbjct: 177 IIGVYGPGGVGKTTLMQSINNELITKGHQY-DVLIWVQMSREFGEC 221
>At3g51520.1 68416.m05642 diacylglycerol acyltransferase family
contains Pfam PF03982: Diacylglycerol acyltransferase
Length = 314
Score = 27.9 bits (59), Expect = 7.6
Identities = 21/67 (31%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
Frame = -2
Query: 359 EHAQPGHPEQHEQRPTQVHPERIVSILQYLLSRHPV-NCRLLASSSIFASPFLWSPTTYF 183
E QP P V P +V++ L P+ N ++LASS+IF +PFL T+
Sbjct: 95 EAFQPNRAYVFGYEPHSVLPIGVVALCD-LTGFMPIPNIKVLASSAIFYTPFLRHIWTWL 153
Query: 182 ASDMSTR 162
++R
Sbjct: 154 GLTAASR 160
>At2g26420.1 68415.m03170 1-phosphatidylinositol-4-phosphate
5-kinase, putative / PIP kinase, putative /
PtdIns(4)P-5-kinase, putative / diphosphoinositide
kinase, putative similar to
phosphatidylinositol-4-phosphate 5-kinase AtPIP5K1
[Arabidopsis thaliana] GI:3702691; contains Pfam
profiles PF01504: Phosphatidylinositol-4-phosphate
5-Kinase, PF02493: MORN repeat
Length = 705
Score = 27.9 bits (59), Expect = 7.6
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = -1
Query: 297 KDRQHTSVPPLSSSRELPFISIEFNLRVTIPVV 199
KD+Q T PP S P +S EF + P+V
Sbjct: 361 KDKQWTRFPPEGSKSTPPHLSAEFKWKDYCPIV 393
>At1g74070.1 68414.m08579 peptidyl-prolyl cis-trans isomerase
cyclophilin-type family protein contains Pfam domain,
PF00160: peptidyl-prolyl cis-trans isomerase,
cyclophilin-type
Length = 317
Score = 27.9 bits (59), Expect = 7.6
Identities = 15/38 (39%), Positives = 21/38 (55%)
Frame = +3
Query: 360 AGAIAVIVRAPKCGPPEPRTWYELGPLVGLELVDAVEP 473
AG++ ++VR P PP+ + G LV E V AV P
Sbjct: 192 AGSVGIVVRDPSKPPPKTKLVARNGKLVVEEEVIAVGP 229
>At1g09980.1 68414.m01126 expressed protein contains Pfam profile
PF05057: Protein of unknown function (DUF676);
non-consensus GC donor splice site at exon boundary
144764
Length = 802
Score = 27.9 bits (59), Expect = 7.6
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +2
Query: 164 VWTCPMRNMSSGTTGMVTRRLNSMLINGNSRDDERG 271
VW P R S + R++S L+NG+ DDE G
Sbjct: 473 VWNAPRRTFSDNSYMRHVDRIDSSLLNGH--DDESG 506
>At5g59130.1 68418.m07411 subtilase family protein contains
similarity to prepro-cucumisin GI:807698 from [Cucumis
melo]
Length = 732
Score = 27.5 bits (58), Expect = 10.0
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = +1
Query: 331 CSGWPGCACSPGLLQSLYALPSAARPNPGHGTNS 432
C+G C+ L+ + + P AR + GHGT++
Sbjct: 167 CAGGKNFTCNNKLIGARHYSPGDARDSTGHGTHT 200
>At2g20590.2 68415.m02408 reticulon family protein non-consensus AA
donor splice site at 46485 and non-consensus GG acceptor
splice site at 46563, confirmed by Ceres cDNA 1885;
contains Pfam profile PF02453: Reticulon
Length = 323
Score = 27.5 bits (58), Expect = 10.0
Identities = 12/20 (60%), Positives = 16/20 (80%)
Frame = -1
Query: 303 PRKDRQHTSVPPLSSSRELP 244
P+KD+Q +S PPL SS +LP
Sbjct: 136 PKKDKQ-SSAPPLCSSSDLP 154
>At2g20590.1 68415.m02407 reticulon family protein non-consensus AA
donor splice site at 46485 and non-consensus GG acceptor
splice site at 46563, confirmed by Ceres cDNA 1885;
contains Pfam profile PF02453: Reticulon
Length = 431
Score = 27.5 bits (58), Expect = 10.0
Identities = 12/20 (60%), Positives = 16/20 (80%)
Frame = -1
Query: 303 PRKDRQHTSVPPLSSSRELP 244
P+KD+Q +S PPL SS +LP
Sbjct: 136 PKKDKQ-SSAPPLCSSSDLP 154
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,801,192
Number of Sequences: 28952
Number of extensions: 295132
Number of successful extensions: 1016
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 969
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1014
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1653386488
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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