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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV30221
         (828 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical...    58   9e-09
At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO) ...    57   1e-08
At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO) ...    48   1e-05
At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO) ...    35   0.076
At1g68185.1 68414.m07789 ubiquitin-related similar to ubiquitin-...    34   0.10 
At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351 ...    34   0.13 
At2g29340.2 68415.m03563 short-chain dehydrogenase/reductase (SD...    29   2.8  
At2g29340.1 68415.m03564 short-chain dehydrogenase/reductase (SD...    29   2.8  
At4g33550.1 68417.m04768 protease inhibitor/seed storage/lipid t...    28   6.6  

>At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical to
           Ubiquitin-like protein SMT3 SP:P55852 from[Arabidopsis
           thaliana]; identical to cDNA SMT3 protein GI:1707371
          Length = 100

 Score = 57.6 bits (133), Expect = 9e-09
 Identities = 28/48 (58%), Positives = 34/48 (70%), Gaps = 1/48 (2%)
 Frame = +1

Query: 367 DEKKGEN-EHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSM 507
           D+K G+   HINLKV GQD   V F+IK+ T L+KLMNAYCDR  + M
Sbjct: 8   DKKPGDGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDM 55



 Score = 39.9 bits (89), Expect = 0.002
 Identities = 16/36 (44%), Positives = 22/36 (61%)
 Frame = +3

Query: 513 VRFRFDGQPINENDTPTSLEMEEGDTIEVYQQQTGG 620
           + F FDG+ +    TP  L+ME+GD I+    QTGG
Sbjct: 58  IAFLFDGRRLRAEQTPDELDMEDGDEIDAMLHQTGG 93


>At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO)
           similar to ubiquitin-like protein SMT3 SP:P55852 from
           [Arabidopsis thaliana]; identical to cDNA small
           ubiquitin-like modifier 2 (SUMO)  GI:22652843; contains
           Pfam profile PF00240: Ubiquitin family
          Length = 103

 Score = 57.2 bits (132), Expect = 1e-08
 Identities = 26/42 (61%), Positives = 31/42 (73%)
 Frame = +1

Query: 367 DEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDR 492
           D+K  +  HINLKV GQD   V F+IK+ T L+KLMNAYCDR
Sbjct: 8   DKKPDQGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDR 49



 Score = 41.1 bits (92), Expect = 9e-04
 Identities = 17/36 (47%), Positives = 22/36 (61%)
 Frame = +3

Query: 513 VRFRFDGQPINENDTPTSLEMEEGDTIEVYQQQTGG 620
           + F FDG+ +    TP  LEME+GD I+    QTGG
Sbjct: 57  IAFLFDGRRLRAEQTPDELEMEDGDEIDAMLHQTGG 92


>At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO)
           similar to SP|O13351 Ubiquitin-like protein smt3/pmt3
           {Schizosaccharomyces pombe}; identical to cDNA small
           ubiquitin-like modifier 3 (SUMO) GI:22652845
          Length = 111

 Score = 47.6 bits (108), Expect = 1e-05
 Identities = 22/42 (52%), Positives = 26/42 (61%)
 Frame = +1

Query: 382 ENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSM 507
           +  H+ LKV  QD   V FK KK  PL+KLM  YCDR GL +
Sbjct: 14  QEAHVILKVKSQDGDEVLFKNKKSAPLKKLMYVYCDRRGLKL 55



 Score = 33.5 bits (73), Expect = 0.18
 Identities = 14/35 (40%), Positives = 22/35 (62%)
 Frame = +3

Query: 519 FRFDGQPINENDTPTSLEMEEGDTIEVYQQQTGGV 623
           F F+G  I   +TP  L+ME+GD I+  +  +GG+
Sbjct: 60  FIFNGARIGGLETPDELDMEDGDVIDACRAMSGGL 94


>At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO)
           similar to ubiquitin-like protein SMT3 SP:P55852
           [Arabidopsis thaliana]; contains INTERPRO:IPR000626
           ubiquitin domain; contains Pfam profile PF00240:
           Ubiquitin family; contains Pfam profile PF00240:
           Ubiquitin family;  identical to cDNA small
           ubiquitin-like modifier 5 (SUMO) mRNA GI:22652847
          Length = 108

 Score = 34.7 bits (76), Expect = 0.076
 Identities = 17/51 (33%), Positives = 26/51 (50%)
 Frame = +1

Query: 349 INLKMADEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGL 501
           ++ K    +   +  + LKV  Q  A   +KI  H  L+KLM+AYC +  L
Sbjct: 13  VSKKSRSPETSPHMKVTLKVKNQQGAEDLYKIGTHAHLKKLMSAYCTKRNL 63



 Score = 31.9 bits (69), Expect = 0.53
 Identities = 15/36 (41%), Positives = 20/36 (55%)
 Frame = +3

Query: 513 VRFRFDGQPINENDTPTSLEMEEGDTIEVYQQQTGG 620
           VRF ++G+ I    TP  L MEE D I +  +  GG
Sbjct: 68  VRFVYNGREIKARQTPAQLHMEEEDEICMVMELGGG 103


>At1g68185.1 68414.m07789 ubiquitin-related similar to
           ubiquitin-like protein smt3/pmt3 SP:O13351 from [Fission
           yeast]
          Length = 215

 Score = 34.3 bits (75), Expect = 0.10
 Identities = 15/32 (46%), Positives = 22/32 (68%)
 Frame = +3

Query: 519 FRFDGQPINENDTPTSLEMEEGDTIEVYQQQT 614
           F FDG  I+ + TP+ L ME+ D IEV+ ++T
Sbjct: 184 FIFDGDKIDPSTTPSELGMEDHDMIEVHTKKT 215


>At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351
           Ubiquitin-like protein smt3/pmt3 {Schizosaccharomyces
           pombe}
          Length = 114

 Score = 33.9 bits (74), Expect = 0.13
 Identities = 16/35 (45%), Positives = 19/35 (54%)
 Frame = +3

Query: 516 RFRFDGQPINENDTPTSLEMEEGDTIEVYQQQTGG 620
           RF FDG  I E  TP  LE ++GD I+    Q  G
Sbjct: 70  RFLFDGSRIREYHTPDELERKDGDEIDAMLCQQSG 104


>At2g29340.2 68415.m03563 short-chain dehydrogenase/reductase (SDR)
           family protein similar to tropinone reductase-I
           GI:424160 from [Datura stramonium]
          Length = 262

 Score = 29.5 bits (63), Expect = 2.8
 Identities = 14/46 (30%), Positives = 25/46 (54%)
 Frame = -1

Query: 168 LTETQKEKAKQYTSECVKESGVSTEVINAAKTGQYSEDKAFKKFVL 31
           L +  K  A ++  + ++ + V+  VIN   +  Y ED +FKK +L
Sbjct: 166 LIQLAKNLACEWAKDGIRANAVAPNVINTPLSQSYLEDVSFKKALL 211


>At2g29340.1 68415.m03564 short-chain dehydrogenase/reductase (SDR)
           family protein similar to tropinone reductase-I
           GI:424160 from [Datura stramonium]
          Length = 307

 Score = 29.5 bits (63), Expect = 2.8
 Identities = 14/46 (30%), Positives = 25/46 (54%)
 Frame = -1

Query: 168 LTETQKEKAKQYTSECVKESGVSTEVINAAKTGQYSEDKAFKKFVL 31
           L +  K  A ++  + ++ + V+  VIN   +  Y ED +FKK +L
Sbjct: 166 LIQLAKNLACEWAKDGIRANAVAPNVINTPLSQSYLEDVSFKKALL 211


>At4g33550.1 68417.m04768 protease inhibitor/seed storage/lipid
           transfer protein (LTP) family protein contains Pfam
           protease inhibitor/seed storage/LTP family domain
           PF00234
          Length = 115

 Score = 28.3 bits (60), Expect = 6.6
 Identities = 11/22 (50%), Positives = 16/22 (72%)
 Frame = -1

Query: 219 LICLAFAVFNCGADNVHLTETQ 154
           L+ LA A+F  G+DNVH+ + Q
Sbjct: 9   LVILAIALFMIGSDNVHVAKAQ 30


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,468,659
Number of Sequences: 28952
Number of extensions: 315456
Number of successful extensions: 745
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 723
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 745
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1902108000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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