Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30215
(677 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g26780.1 68418.m03193 glycine hydroxymethyltransferase, putat... 80 2e-15
At5g26780.3 68418.m03195 glycine hydroxymethyltransferase, putat... 79 4e-15
At5g26780.2 68418.m03194 glycine hydroxymethyltransferase, putat... 79 4e-15
At4g37930.1 68417.m05363 glycine hydroxymethyltransferase / seri... 77 1e-14
At4g32520.1 68417.m04629 glycine hydroxymethyltransferase, putat... 76 2e-14
At4g13930.1 68417.m02156 glycine hydroxymethyltransferase, putat... 72 4e-13
At4g13890.1 68417.m02152 glycine hydroxymethyltransferase, putat... 70 1e-12
At1g36370.1 68414.m04518 glycine hydroxymethyltransferase, putat... 64 6e-11
At1g22020.1 68414.m02755 glycine hydroxymethyltransferase, putat... 62 2e-10
At2g34610.1 68415.m04252 expressed protein 30 1.6
At1g79280.1 68414.m09242 expressed protein weak similarity to Nu... 28 6.5
At1g04470.1 68414.m00438 expressed protein EST gb|ATTS5672 comes... 28 6.5
At1g01090.1 68414.m00011 pyruvate dehydrogenase E1 component alp... 27 8.6
>At5g26780.1 68418.m03193 glycine hydroxymethyltransferase, putative
/ serine hydroxymethyltransferase, putative /
serine/threonine aldolase, putative strong similarity to
SP|P50433 Serine hydroxymethyltransferase, mitochondrial
precursor (EC 2.1.2.1) (Serine methylase) (Glycine
hydroxymethyltransferase) (SHMT) {Solanum tuberosum};
contains Pfam profile PF00464: serine
hydroxymethyltransferase
Length = 517
Score = 79.8 bits (188), Expect = 2e-15
Identities = 34/64 (53%), Positives = 48/64 (75%)
Frame = +3
Query: 63 EGLISRGYSIATGGTDVHLALVDLRGVGLRGAPAERVLELCSVACNKNTVPGDIGALNPS 242
E L+++GY + +GGTD HL LV+L+ G+ G+ E+VLEL +A NKNTVPGD+ A+ P
Sbjct: 368 ETLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGDVSAMVPG 427
Query: 243 GIRL 254
GIR+
Sbjct: 428 GIRM 431
Score = 63.7 bits (148), Expect = 1e-10
Identities = 33/78 (42%), Positives = 47/78 (60%), Gaps = 1/78 (1%)
Frame = +2
Query: 257 TPALTTRGLKEADIDIVVDFIDRALKXGLEIIKVS-GLKLVDFNKAIEENAEFKKKIENL 433
TPALT+RG E D V ++ D A+K L+I S G KL DF ++ N + + ++ L
Sbjct: 433 TPALTSRGFIEEDFAKVAEYFDLAVKIALKIKAESQGTKLKDFVATMQSNEKLQSEMSKL 492
Query: 434 KEEVENYSKSFPLPGFDK 487
+E VE Y+K FP GF+K
Sbjct: 493 REMVEEYAKQFPTIGFEK 510
>At5g26780.3 68418.m03195 glycine hydroxymethyltransferase, putative
/ serine hydroxymethyltransferase, putative /
serine/threonine aldolase, putative strong similarity to
SP|P50433 Serine hydroxymethyltransferase, mitochondrial
precursor (EC 2.1.2.1) (Serine methylase) (Glycine
hydroxymethyltransferase) (SHMT) {Solanum tuberosum};
contains Pfam profile PF00464: serine
hydroxymethyltransferase
Length = 533
Score = 78.6 bits (185), Expect = 4e-15
Identities = 33/62 (53%), Positives = 47/62 (75%)
Frame = +3
Query: 69 LISRGYSIATGGTDVHLALVDLRGVGLRGAPAERVLELCSVACNKNTVPGDIGALNPSGI 248
L+++GY + +GGTD HL LV+L+ G+ G+ E+VLEL +A NKNTVPGD+ A+ P GI
Sbjct: 386 LLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGDVSAMVPGGI 445
Query: 249 RL 254
R+
Sbjct: 446 RM 447
Score = 63.7 bits (148), Expect = 1e-10
Identities = 33/78 (42%), Positives = 47/78 (60%), Gaps = 1/78 (1%)
Frame = +2
Query: 257 TPALTTRGLKEADIDIVVDFIDRALKXGLEIIKVS-GLKLVDFNKAIEENAEFKKKIENL 433
TPALT+RG E D V ++ D A+K L+I S G KL DF ++ N + + ++ L
Sbjct: 449 TPALTSRGFIEEDFAKVAEYFDLAVKIALKIKAESQGTKLKDFVATMQSNEKLQSEMSKL 508
Query: 434 KEEVENYSKSFPLPGFDK 487
+E VE Y+K FP GF+K
Sbjct: 509 REMVEEYAKQFPTIGFEK 526
>At5g26780.2 68418.m03194 glycine hydroxymethyltransferase, putative
/ serine hydroxymethyltransferase, putative /
serine/threonine aldolase, putative strong similarity to
SP|P50433 Serine hydroxymethyltransferase, mitochondrial
precursor (EC 2.1.2.1) (Serine methylase) (Glycine
hydroxymethyltransferase) (SHMT) {Solanum tuberosum};
contains Pfam profile PF00464: serine
hydroxymethyltransferase
Length = 533
Score = 78.6 bits (185), Expect = 4e-15
Identities = 33/62 (53%), Positives = 47/62 (75%)
Frame = +3
Query: 69 LISRGYSIATGGTDVHLALVDLRGVGLRGAPAERVLELCSVACNKNTVPGDIGALNPSGI 248
L+++GY + +GGTD HL LV+L+ G+ G+ E+VLEL +A NKNTVPGD+ A+ P GI
Sbjct: 386 LLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGDVSAMVPGGI 445
Query: 249 RL 254
R+
Sbjct: 446 RM 447
Score = 63.7 bits (148), Expect = 1e-10
Identities = 33/78 (42%), Positives = 47/78 (60%), Gaps = 1/78 (1%)
Frame = +2
Query: 257 TPALTTRGLKEADIDIVVDFIDRALKXGLEIIKVS-GLKLVDFNKAIEENAEFKKKIENL 433
TPALT+RG E D V ++ D A+K L+I S G KL DF ++ N + + ++ L
Sbjct: 449 TPALTSRGFIEEDFAKVAEYFDLAVKIALKIKAESQGTKLKDFVATMQSNEKLQSEMSKL 508
Query: 434 KEEVENYSKSFPLPGFDK 487
+E VE Y+K FP GF+K
Sbjct: 509 REMVEEYAKQFPTIGFEK 526
>At4g37930.1 68417.m05363 glycine hydroxymethyltransferase / serine
hydroxymethyltransferase / serine/threonine aldolase
(SHM1) identical to serine hydroxymethyl transferase
[Arabidopsis thaliana] GI:6899945
Length = 517
Score = 77.0 bits (181), Expect = 1e-14
Identities = 34/68 (50%), Positives = 47/68 (69%)
Frame = +3
Query: 51 AALCEGLISRGYSIATGGTDVHLALVDLRGVGLRGAPAERVLELCSVACNKNTVPGDIGA 230
A + L+ RGY + +GGTD HL LV+L+ G+ G+ E+VLE +A NKNTVPGD+ A
Sbjct: 364 AKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKVLEAVHIASNKNTVPGDVSA 423
Query: 231 LNPSGIRL 254
+ P GIR+
Sbjct: 424 MVPGGIRM 431
Score = 64.5 bits (150), Expect = 6e-11
Identities = 31/78 (39%), Positives = 48/78 (61%), Gaps = 1/78 (1%)
Frame = +2
Query: 257 TPALTTRGLKEADIDIVVDFIDRALKXGLEI-IKVSGLKLVDFNKAIEENAEFKKKIENL 433
TPALT+RG E D V ++ D+A+ L++ + G KL DF A+E ++ + +I L
Sbjct: 433 TPALTSRGFVEEDFAKVAEYFDKAVTIALKVKSEAQGTKLKDFVSAMESSSTIQSEIAKL 492
Query: 434 KEEVENYSKSFPLPGFDK 487
+ EVE ++K FP GF+K
Sbjct: 493 RHEVEEFAKQFPTIGFEK 510
>At4g32520.1 68417.m04629 glycine hydroxymethyltransferase, putative
/ serine hydroxymethyltransferase, putative /
serine/threonine aldolase, putative similar to serine
hydroxymethyltransferase [Chlamydomonas reinhardtii]
GI:17066746; contains Pfam profile PF00464: serine
hydroxymethyltransferase
Length = 529
Score = 75.8 bits (178), Expect = 2e-14
Identities = 33/67 (49%), Positives = 47/67 (70%)
Frame = +3
Query: 54 ALCEGLISRGYSIATGGTDVHLALVDLRGVGLRGAPAERVLELCSVACNKNTVPGDIGAL 233
AL L+ G+ + +GG+D HL LVDLR +G+ GA E++L++ S+ NKN+VPGD AL
Sbjct: 385 ALANRLVELGFKLVSGGSDNHLVLVDLRPMGMDGARVEKILDMASITLNKNSVPGDKSAL 444
Query: 234 NPSGIRL 254
P GIR+
Sbjct: 445 VPGGIRI 451
Score = 65.7 bits (153), Expect = 3e-11
Identities = 34/77 (44%), Positives = 51/77 (66%), Gaps = 3/77 (3%)
Frame = +2
Query: 257 TPALTTRGLKEADIDIVVDFIDRALKXGLEIIKVS-GLKLVDFNKAIEENAEF--KKKIE 427
+PA+TTRGL E D +V DFI ++ +E K + G KL DFNK + + EF K++++
Sbjct: 453 SPAMTTRGLSEKDFVVVADFIKEGVEITMEAKKAAPGSKLQDFNKFV-TSPEFPLKERVK 511
Query: 428 NLKEEVENYSKSFPLPG 478
+LKE VE ++ FP+PG
Sbjct: 512 SLKERVETFTSRFPIPG 528
>At4g13930.1 68417.m02156 glycine hydroxymethyltransferase, putative
/ serine hydroxymethyltransferase, putative /
serine/threonine aldolase, putative similar to serine
hydroxymethyltransferase [Chlamydomonas reinhardtii]
GI:17066746; contains Pfam profile PF00464: serine
hydroxymethyltransferase
Length = 471
Score = 71.7 bits (168), Expect = 4e-13
Identities = 34/67 (50%), Positives = 44/67 (65%)
Frame = +3
Query: 54 ALCEGLISRGYSIATGGTDVHLALVDLRGVGLRGAPAERVLELCSVACNKNTVPGDIGAL 233
AL L+S+GY I T GT+ HL L DLR +GL G E++ +LCS+ NKN V GD AL
Sbjct: 324 ALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGNKVEKLCDLCSITLNKNAVFGDSSAL 383
Query: 234 NPSGIRL 254
P G+R+
Sbjct: 384 APGGVRI 390
Score = 61.7 bits (143), Expect = 4e-10
Identities = 31/74 (41%), Positives = 46/74 (62%)
Frame = +2
Query: 260 PALTTRGLKEADIDIVVDFIDRALKXGLEIIKVSGLKLVDFNKAIEENAEFKKKIENLKE 439
PA+T+RGL E D + + +F+ RA+ L+I K G L DFNK + N K ++ LK
Sbjct: 393 PAMTSRGLVEKDFEQIGEFLSRAVTLTLDIQKTYGKLLKDFNKGLVNN----KDLDQLKA 448
Query: 440 EVENYSKSFPLPGF 481
+VE +S S+ +PGF
Sbjct: 449 DVEKFSASYEMPGF 462
>At4g13890.1 68417.m02152 glycine hydroxymethyltransferase, putative
/ serine hydroxymethyltransferase, putative /
serine/threonine aldolase, putative similar to serine
hydroxymethyltransferase [Chlamydomonas reinhardtii]
GI:17066746; contains Pfam profile PF00464: serine
hydroxymethyltransferase
Length = 470
Score = 70.1 bits (164), Expect = 1e-12
Identities = 33/68 (48%), Positives = 43/68 (63%)
Frame = +3
Query: 51 AALCEGLISRGYSIATGGTDVHLALVDLRGVGLRGAPAERVLELCSVACNKNTVPGDIGA 230
A L LI++GY++ T GTD HL L DLR +GL G E+V ELC + N+N V GD
Sbjct: 323 ACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGNKVEKVCELCYITLNRNAVFGDTSF 382
Query: 231 LNPSGIRL 254
L P G+R+
Sbjct: 383 LAPGGVRI 390
Score = 58.8 bits (136), Expect = 3e-09
Identities = 29/75 (38%), Positives = 47/75 (62%)
Frame = +2
Query: 257 TPALTTRGLKEADIDIVVDFIDRALKXGLEIIKVSGLKLVDFNKAIEENAEFKKKIENLK 436
TPA+T+RGL E D + + +F+ RA+ L+I + G + DFNK + N K+I+ +K
Sbjct: 392 TPAMTSRGLVEKDFEKIGEFLHRAVTITLDIQEQYGKVMKDFNKGLVNN----KEIDEIK 447
Query: 437 EEVENYSKSFPLPGF 481
+VE ++ F +PGF
Sbjct: 448 ADVEEFTYDFDMPGF 462
>At1g36370.1 68414.m04518 glycine hydroxymethyltransferase, putative
/ serine hydroxymethyltransferase, putative /
serine/threonine aldolase, putative similar to serine
hydroxymethyltransferase [Chlamydomonas reinhardtii]
GI:17066746; contains Pfam profile PF00464: serine
hydroxymethyltransferase
Length = 598
Score = 64.5 bits (150), Expect = 6e-11
Identities = 30/71 (42%), Positives = 41/71 (57%)
Frame = +3
Query: 42 KERAALCEGLISRGYSIATGGTDVHLALVDLRGVGLRGAPAERVLELCSVACNKNTVPGD 221
K AL L+ R + TGGTD HL L DL +GL G E+V E+C + NK + GD
Sbjct: 451 KNAQALAAALLRRKCRLVTGGTDNHLLLWDLTPMGLTGKVYEKVCEMCHITLNKTAIFGD 510
Query: 222 IGALNPSGIRL 254
G ++P G+R+
Sbjct: 511 NGTISPGGVRI 521
Score = 39.1 bits (87), Expect = 0.003
Identities = 22/73 (30%), Positives = 39/73 (53%)
Frame = +2
Query: 257 TPALTTRGLKEADIDIVVDFIDRALKXGLEIIKVSGLKLVDFNKAIEENAEFKKKIENLK 436
TPA+TTRG E+D + + DF+ +A + + + G +F K++ N K I L+
Sbjct: 523 TPAMTTRGCIESDFETMADFLIKAAQITSALQREHGKSHKEFVKSLCTN----KDIAELR 578
Query: 437 EEVENYSKSFPLP 475
VE ++ + +P
Sbjct: 579 NRVEAFALQYEMP 591
>At1g22020.1 68414.m02755 glycine hydroxymethyltransferase, putative
/ serine hydroxymethyltransferase, putative /
serine/threonine aldolase, putative similar to serine
hydroxymethyltransferase [Chlamydomonas reinhardtii]
GI:17066746; contains Pfam profile PF00464: serine
hydroxymethyltransferase
Length = 599
Score = 62.5 bits (145), Expect = 2e-10
Identities = 30/71 (42%), Positives = 41/71 (57%)
Frame = +3
Query: 42 KERAALCEGLISRGYSIATGGTDVHLALVDLRGVGLRGAPAERVLELCSVACNKNTVPGD 221
K AL LISR + TGGTD HL L DL +GL G E+V E+C + NK + +
Sbjct: 455 KNAKALASALISRKCKLITGGTDNHLLLWDLTPLGLTGKVYEKVCEMCHITVNKVAIFSE 514
Query: 222 IGALNPSGIRL 254
G ++P G+R+
Sbjct: 515 NGVISPGGVRI 525
Score = 37.5 bits (83), Expect = 0.008
Identities = 23/76 (30%), Positives = 41/76 (53%)
Frame = +2
Query: 257 TPALTTRGLKEADIDIVVDFIDRALKXGLEIIKVSGLKLVDFNKAIEENAEFKKKIENLK 436
+PA+T+RG E + + + DF+ RA + + G KL K ++ K+I +L+
Sbjct: 527 SPAMTSRGCLEPEFETMADFLYRAAQIASAAQREHG-KL---QKEPLKSIYHCKEIADLR 582
Query: 437 EEVENYSKSFPLPGFD 484
+VE ++ F +P FD
Sbjct: 583 NQVEAFATQFAMPAFD 598
>At2g34610.1 68415.m04252 expressed protein
Length = 290
Score = 29.9 bits (64), Expect = 1.6
Identities = 15/25 (60%), Positives = 17/25 (68%)
Frame = +2
Query: 386 KAIEENAEFKKKIENLKEEVENYSK 460
KA EE E KK EN+KEE E +SK
Sbjct: 241 KADEEMEEATKKWENVKEESEEFSK 265
>At1g79280.1 68414.m09242 expressed protein weak similarity to
Nucleoprotein TPR (Swiss-Prot:P12270) [Homo sapiens]
Length = 2111
Score = 27.9 bits (59), Expect = 6.5
Identities = 11/36 (30%), Positives = 21/36 (58%)
Frame = +2
Query: 344 EIIKVSGLKLVDFNKAIEENAEFKKKIENLKEEVEN 451
+II LK D N +E+N + + + +L E++E+
Sbjct: 559 KIISEHLLKFKDINGLVEQNVKLRNLVRSLSEQIES 594
>At1g04470.1 68414.m00438 expressed protein EST gb|ATTS5672 comes
from this gene
Length = 1035
Score = 27.9 bits (59), Expect = 6.5
Identities = 15/50 (30%), Positives = 30/50 (60%)
Frame = +2
Query: 299 DIVVDFIDRALKXGLEIIKVSGLKLVDFNKAIEENAEFKKKIENLKEEVE 448
D+ + ++ K ++++ SG ++ + +A +NA F K IEN+K E+E
Sbjct: 460 DVTISQMNGLEKGDVKLVDSSGDRVDYYIRASIKNA-FSKVIENMKAEIE 508
>At1g01090.1 68414.m00011 pyruvate dehydrogenase E1 component alpha
subunit, chloroplast identical to pyruvate dehydrogenase
E1 alpha subunit GB:AAB86803 GI:2454182 from
[Arabidopsis thaliana]; identical to cDNA pyruvate
dehydrogenase E1 alpha subunit mRNA, nuclear gene
encoding plastid protein GI:2454181
Length = 428
Score = 27.5 bits (58), Expect = 8.6
Identities = 21/69 (30%), Positives = 32/69 (46%), Gaps = 1/69 (1%)
Frame = +2
Query: 275 RGLKEADIDIVVDFIDRALKXGLEIIKVSGLKLVDFNKAIE-ENAEFKKKIENLKEEVEN 451
RG AD D + D ++A + I L++ A E E +KKI+ L EE
Sbjct: 323 RGHSLADPDELRDAAEKAKYAARDPIAALKKYLIENKLAKEAELKSIEKKIDELVEEAVE 382
Query: 452 YSKSFPLPG 478
++ + P PG
Sbjct: 383 FADASPQPG 391
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,585,274
Number of Sequences: 28952
Number of extensions: 173245
Number of successful extensions: 570
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 537
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 565
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1428369392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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