BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV30208 (550 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g42270.1 68415.m05232 U5 small nuclear ribonucleoprotein heli... 58 5e-09 At1g20960.1 68414.m02624 U5 small nuclear ribonucleoprotein heli... 51 6e-07 At3g28870.1 68416.m03604 hypothetical protein 40 0.001 At3g59950.2 68416.m06690 autophagy 4b (APG4b) identical to autop... 29 2.7 At3g59950.1 68416.m06691 autophagy 4b (APG4b) identical to autop... 29 2.7 At5g24530.1 68418.m02897 oxidoreductase, 2OG-Fe(II) oxygenase fa... 27 6.2 At4g24860.1 68417.m03559 AAA-type ATPase family protein contains... 27 6.2 At5g61140.1 68418.m07670 DEAD box RNA helicase, putative similar... 27 8.3 At3g04050.1 68416.m00427 pyruvate kinase, putative similar to py... 27 8.3 At2g29920.1 68415.m03634 expressed protein 27 8.3 >At2g42270.1 68415.m05232 U5 small nuclear ribonucleoprotein helicase, putative Length = 2172 Score = 57.6 bits (133), Expect = 5e-09 Identities = 28/82 (34%), Positives = 47/82 (57%), Gaps = 2/82 (2%) Frame = +2 Query: 227 PARQERGLVLTLGTVDDGELHALKRVPPRGTAQ--LTFYTPSRTGRIIYTVYIMSDSYMG 400 P +E G L +G +L A+KR+ + AQ L F P+ TG YT+Y M DSY+G Sbjct: 2091 PKTKEEGWWLVVGEAKTNQLMAIKRISLQRKAQVKLEFAVPTETGEKSYTLYFMCDSYLG 2150 Query: 401 LDQQYDLQFEIIDAMPSENVDK 466 DQ+Y ++ D+ ++++++ Sbjct: 2151 CDQEYSFTVDVKDSDAADHMEE 2172 >At1g20960.1 68414.m02624 U5 small nuclear ribonucleoprotein helicase, putative similar to SP|O75643 U5 small nuclear ribonucleoprotein 200 kDa helicase {Homo sapiens}; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain, PF02889: Sec63 domain Length = 2171 Score = 50.8 bits (116), Expect = 6e-07 Identities = 26/71 (36%), Positives = 37/71 (52%), Gaps = 2/71 (2%) Frame = +2 Query: 227 PARQERGLVLTLGTVDDGELHALKRVP--PRGTAQLTFYTPSRTGRIIYTVYIMSDSYMG 400 P +E G L +G +L A+KRV + +L F PS G YT+Y M DSY+G Sbjct: 2090 PKTKEEGWWLVVGDTKTNQLLAIKRVSLQRKVKVKLDFTAPSEPGEKSYTLYFMCDSYLG 2149 Query: 401 LDQQYDLQFEI 433 DQ+Y ++ Sbjct: 2150 CDQEYSFSVDV 2160 >At3g28870.1 68416.m03604 hypothetical protein Length = 355 Score = 39.9 bits (89), Expect = 0.001 Identities = 16/40 (40%), Positives = 22/40 (55%) Frame = +2 Query: 311 RGTAQLTFYTPSRTGRIIYTVYIMSDSYMGLDQQYDLQFE 430 + +L F PS G YT+Y M DSY+G DQ+Y + Sbjct: 304 KAKVKLDFTVPSEPGEKSYTLYFMCDSYLGCDQEYSFSVD 343 >At3g59950.2 68416.m06690 autophagy 4b (APG4b) identical to autophagy 4b [Arabidopsis thaliana] gi|19912145|dbj|BAB88384 Length = 471 Score = 28.7 bits (61), Expect = 2.7 Identities = 13/48 (27%), Positives = 25/48 (52%) Frame = -2 Query: 165 YIPDQHAKASTDPGREVA*SVCLVHDQRRTIDRGRATLCP*LVGRISP 22 ++P + + +ST R+++ LV D + ++ TLC +V SP Sbjct: 8 FVPSKCSSSSTSEKRDISSPTSLVSDSASSDNKSNLTLCSDVVASSSP 55 >At3g59950.1 68416.m06691 autophagy 4b (APG4b) identical to autophagy 4b [Arabidopsis thaliana] gi|19912145|dbj|BAB88384 Length = 477 Score = 28.7 bits (61), Expect = 2.7 Identities = 13/48 (27%), Positives = 25/48 (52%) Frame = -2 Query: 165 YIPDQHAKASTDPGREVA*SVCLVHDQRRTIDRGRATLCP*LVGRISP 22 ++P + + +ST R+++ LV D + ++ TLC +V SP Sbjct: 8 FVPSKCSSSSTSEKRDISSPTSLVSDSASSDNKSNLTLCSDVVASSSP 55 >At5g24530.1 68418.m02897 oxidoreductase, 2OG-Fe(II) oxygenase family protein similar to flavanone 3-hydroxylase [Persea americana][GI:727410]; contains PF03171 2OG-Fe(II) oxygenase superfamily domain Length = 341 Score = 27.5 bits (58), Expect = 6.2 Identities = 16/71 (22%), Positives = 31/71 (43%) Frame = +2 Query: 272 DDGELHALKRVPPRGTAQLTFYTPSRTGRIIYTVYIMSDSYMGLDQQYDLQFEIIDAMPS 451 + G+ A+ PP +LT+ P+ T T+ + + GL D Q+ ++ P Sbjct: 187 EQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPD 246 Query: 452 ENVDKVYDTIE 484 V + D ++ Sbjct: 247 AFVINIGDQLQ 257 >At4g24860.1 68417.m03559 AAA-type ATPase family protein contains Pfam profile PF00004: ATPase, AAA family Length = 1122 Score = 27.5 bits (58), Expect = 6.2 Identities = 13/43 (30%), Positives = 23/43 (53%) Frame = -1 Query: 265 AERQNQPSFLPRGNLGHSTLFGLPPLRCISNSRVYS*SACKGI 137 ++ Q +P LPR ++ H+ G+PPL C+ + S G+ Sbjct: 466 SQLQLEPETLPR-SVNHTLKKGMPPLHCLQQKILLQSSWISGL 507 >At5g61140.1 68418.m07670 DEAD box RNA helicase, putative similar to ASC-1 complex subunit P200 [Homo sapiens] GI:12061185; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain, PF02889: Sec63 domain Length = 2146 Score = 27.1 bits (57), Expect = 8.3 Identities = 9/19 (47%), Positives = 13/19 (68%) Frame = +3 Query: 198 NPNNVLCPRFPRGKNEGWF 254 N + L PRFP+ K+E W+ Sbjct: 2064 NSSRALAPRFPKVKDEAWW 2082 >At3g04050.1 68416.m00427 pyruvate kinase, putative similar to pyruvate kinase, cytosolic isozyme [Nicotiana tabacum] SWISS-PROT:Q42954 Length = 510 Score = 27.1 bits (57), Expect = 8.3 Identities = 19/56 (33%), Positives = 27/56 (48%) Frame = -2 Query: 480 IVSYTLSTFSDGIASIISNCKSYCWSKPM*LSLIIYTVYIIRPVREGV*NVSCAVP 313 IVS LS AS +S +S C + + L+ YTV ++ R V +S VP Sbjct: 367 IVSLPLSPIESLAASAVSTARSLCAAAIVVLTKGGYTVELVAKYRPSVPILSVIVP 422 >At2g29920.1 68415.m03634 expressed protein Length = 187 Score = 27.1 bits (57), Expect = 8.3 Identities = 15/48 (31%), Positives = 23/48 (47%) Frame = +3 Query: 33 DLPTTDIKLHVRGLWFDADHEQDKRITQPPARDQWMPLHADQEYTLLL 176 +L TD L + L DH+ + +T + +PLH D + LLL Sbjct: 98 ELLLTDSDLDLELLLLHQDHDLELLLTDSDFDLELLPLHQDHDLELLL 145 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,666,397 Number of Sequences: 28952 Number of extensions: 240365 Number of successful extensions: 574 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 562 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 574 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1033331880 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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