Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30188
(792 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) riboso... 137 9e-33
At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A) 132 3e-31
At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) riboso... 131 4e-31
At2g44065.2 68415.m05480 ribosomal protein L2 family protein sim... 35 0.071
At2g44065.1 68415.m05479 ribosomal protein L2 family protein sim... 35 0.071
At2g26420.1 68415.m03170 1-phosphatidylinositol-4-phosphate 5-ki... 30 1.5
At2g43660.2 68415.m05427 glycosyl hydrolase family protein 17 si... 29 2.7
At2g43660.1 68415.m05426 glycosyl hydrolase family protein 17 si... 29 2.7
At2g02610.1 68415.m00200 DC1 domain-containing protein contain... 29 2.7
At4g14250.1 68417.m02198 UBX domain-containing protein low simil... 29 4.7
At2g16365.2 68415.m06033 F-box family protein contains Pfam:PF00... 29 4.7
At2g16365.1 68415.m06032 F-box family protein contains Pfam:PF00... 29 4.7
At1g11060.1 68414.m01267 expressed protein 29 4.7
At5g65290.1 68418.m08212 LMBR1 integral membrane family protein ... 28 6.2
At1g33110.1 68414.m04089 MATE efflux family protein similar to r... 28 6.2
At3g27670.1 68416.m03455 expressed protein 28 8.2
>At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) ribosomal
protein L8, cytosolic - Arabidopsis thaliana, PIR:T04582
Length = 260
Score = 137 bits (331), Expect = 9e-33
Identities = 60/83 (72%), Positives = 70/83 (84%)
Frame = +3
Query: 516 MGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIVAGGGRIDKPI 695
+GDRG LARASG++A VI HNP++ TRVKLPSG+KK+LPS+ R M+G VAGGGR +KP
Sbjct: 121 VGDRGALARASGDYAIVIAHNPESNTTRVKLPSGSKKILPSACRAMIGQVAGGGRTEKPF 180
Query: 696 LKAGRAYHKYKVKRNCWPYVRGV 764
LKAG AYHKYK KRNCWP VRGV
Sbjct: 181 LKAGNAYHKYKAKRNCWPVVRGV 203
Score = 78.6 bits (185), Expect = 4e-15
Identities = 33/50 (66%), Positives = 42/50 (84%)
Frame = +2
Query: 254 LDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELFIAPEG 403
LDY ER+GY+KG+V +IIHDPGRGAPLA V FR P+++ +KELF+A EG
Sbjct: 33 LDYGERNGYLKGLVTEIIHDPGRGAPLARVAFRHPFRYMKQKELFVAAEG 82
Score = 64.5 bits (150), Expect = 8e-11
Identities = 23/36 (63%), Positives = 33/36 (91%)
Frame = +1
Query: 403 LYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLE 510
+YTGQ++YCGKKA L VGNV+P+G++PEG ++CN+E
Sbjct: 83 MYTGQYLYCGKKANLMVGNVLPLGSIPEGAVICNVE 118
Score = 47.6 bits (108), Expect = 9e-06
Identities = 25/32 (78%), Positives = 25/32 (78%), Gaps = 1/32 (3%)
Frame = +3
Query: 162 MGRVIRAQRKGA-GSVFVSHTKKRKGAPKLRS 254
MGRVIRAQRKGA GSVF SHT RKG K RS
Sbjct: 1 MGRVIRAQRKGAAGSVFKSHTHHRKGPAKFRS 32
>At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A)
Length = 258
Score = 132 bits (318), Expect = 3e-31
Identities = 58/83 (69%), Positives = 70/83 (84%)
Frame = +3
Query: 516 MGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIVAGGGRIDKPI 695
+GDRG LARASG++A VI HNPD+ TR+KLPSG+KK++PS R M+G VAGGGR +KP+
Sbjct: 120 VGDRGVLARASGDYAIVIAHNPDSDTTRIKLPSGSKKIVPSGCRAMIGQVAGGGRTEKPM 179
Query: 696 LKAGRAYHKYKVKRNCWPYVRGV 764
LKAG AYHKY+VKRN WP VRGV
Sbjct: 180 LKAGNAYHKYRVKRNSWPKVRGV 202
Score = 81.8 bits (193), Expect = 5e-16
Identities = 35/50 (70%), Positives = 43/50 (86%)
Frame = +2
Query: 254 LDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELFIAPEG 403
LD+ ER+GY+KGVV +IIHDPGRGAPLA V FR P++FK +KELF+A EG
Sbjct: 32 LDFGERNGYLKGVVTEIIHDPGRGAPLARVTFRHPFRFKKQKELFVAAEG 81
Score = 64.9 bits (151), Expect = 6e-11
Identities = 25/36 (69%), Positives = 33/36 (91%)
Frame = +1
Query: 403 LYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLE 510
+YTGQF+YCGKKATL VGNV+P+ ++PEG +VCN+E
Sbjct: 82 MYTGQFLYCGKKATLVVGNVLPLRSIPEGAVVCNVE 117
Score = 52.0 bits (119), Expect = 4e-07
Identities = 25/31 (80%), Positives = 25/31 (80%)
Frame = +3
Query: 162 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRS 254
MGRVIRAQRKGAGSVF SHT RKG K RS
Sbjct: 1 MGRVIRAQRKGAGSVFKSHTHHRKGPAKFRS 31
>At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) ribosomal
protein L8, cytosolic, tomato, PIR1:R5TOL8
Length = 258
Score = 131 bits (317), Expect = 4e-31
Identities = 57/83 (68%), Positives = 69/83 (83%)
Frame = +3
Query: 516 MGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIVAGGGRIDKPI 695
+GDRG ARASG++A VI HNPD +R+KLPSG+KK++PS R M+G VAGGGR +KP+
Sbjct: 120 VGDRGVFARASGDYAIVIAHNPDNDTSRIKLPSGSKKIVPSGCRAMIGQVAGGGRTEKPM 179
Query: 696 LKAGRAYHKYKVKRNCWPYVRGV 764
LKAG AYHKY+VKRNCWP VRGV
Sbjct: 180 LKAGNAYHKYRVKRNCWPKVRGV 202
Score = 81.8 bits (193), Expect = 5e-16
Identities = 35/50 (70%), Positives = 43/50 (86%)
Frame = +2
Query: 254 LDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELFIAPEG 403
LD+ ER+GY+KGVV +IIHDPGRGAPLA V FR P++FK +KELF+A EG
Sbjct: 32 LDFGERNGYLKGVVTEIIHDPGRGAPLARVAFRHPFRFKKQKELFVAAEG 81
Score = 64.5 bits (150), Expect = 8e-11
Identities = 24/36 (66%), Positives = 33/36 (91%)
Frame = +1
Query: 403 LYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLE 510
+YTGQF+YCGKKATL VGNV+P+ ++PEG ++CN+E
Sbjct: 82 MYTGQFLYCGKKATLVVGNVLPLRSIPEGAVICNVE 117
Score = 52.0 bits (119), Expect = 4e-07
Identities = 25/31 (80%), Positives = 25/31 (80%)
Frame = +3
Query: 162 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRS 254
MGRVIRAQRKGAGSVF SHT RKG K RS
Sbjct: 1 MGRVIRAQRKGAGSVFKSHTHHRKGPAKFRS 31
>At2g44065.2 68415.m05480 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 34.7 bits (76), Expect = 0.071
Identities = 19/65 (29%), Positives = 33/65 (50%)
Frame = +3
Query: 519 GDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIVAGGGRIDKPIL 698
G ++ RA+G A ++ P + +KLPSG K + + R +G V+ K +
Sbjct: 82 GQGAKMVRAAGTNAKIL-KEPAKGKCLIKLPSGDTKWINAKCRATIGTVSNPSHGTKKLY 140
Query: 699 KAGRA 713
KAG++
Sbjct: 141 KAGQS 145
>At2g44065.1 68415.m05479 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 34.7 bits (76), Expect = 0.071
Identities = 19/65 (29%), Positives = 33/65 (50%)
Frame = +3
Query: 519 GDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIVAGGGRIDKPIL 698
G ++ RA+G A ++ P + +KLPSG K + + R +G V+ K +
Sbjct: 82 GQGAKMVRAAGTNAKIL-KEPAKGKCLIKLPSGDTKWINAKCRATIGTVSNPSHGTKKLY 140
Query: 699 KAGRA 713
KAG++
Sbjct: 141 KAGQS 145
>At2g26420.1 68415.m03170 1-phosphatidylinositol-4-phosphate
5-kinase, putative / PIP kinase, putative /
PtdIns(4)P-5-kinase, putative / diphosphoinositide
kinase, putative similar to
phosphatidylinositol-4-phosphate 5-kinase AtPIP5K1
[Arabidopsis thaliana] GI:3702691; contains Pfam
profiles PF01504: Phosphatidylinositol-4-phosphate
5-Kinase, PF02493: MORN repeat
Length = 705
Score = 30.3 bits (65), Expect = 1.5
Identities = 13/38 (34%), Positives = 24/38 (63%), Gaps = 1/38 (2%)
Frame = -1
Query: 462 HISNFKSCFLSTINKLACVEPSGAMKSSFLVL-NLYGS 352
H+S +K+ ++ + CV+P G K+ F+V+ NL+ S
Sbjct: 462 HVSKYKNSLVTKFFGVHCVKPVGGQKTRFIVMGNLFCS 499
>At2g43660.2 68415.m05427 glycosyl hydrolase family protein 17
similar to glucan endo-1,3-beta-glucosidase precursor
SP:P52409 from [Triticum aestivum]; C terminal homology
only
Length = 123
Score = 29.5 bits (63), Expect = 2.7
Identities = 15/43 (34%), Positives = 21/43 (48%)
Frame = -1
Query: 414 ACVEPSGAMKSSFLVLNLYGSRKCTTAKGAPLPGSWIISLTTP 286
AC +P S+ +V+NLY + GS IIS+T P
Sbjct: 70 ACYDPINLYNSASVVMNLYYQNQGRQYSKCDFEGSGIISVTDP 112
>At2g43660.1 68415.m05426 glycosyl hydrolase family protein 17
similar to glucan endo-1,3-beta-glucosidase precursor
SP:P52409 from [Triticum aestivum]; C terminal homology
only
Length = 122
Score = 29.5 bits (63), Expect = 2.7
Identities = 15/43 (34%), Positives = 21/43 (48%)
Frame = -1
Query: 414 ACVEPSGAMKSSFLVLNLYGSRKCTTAKGAPLPGSWIISLTTP 286
AC +P S+ +V+NLY + GS IIS+T P
Sbjct: 69 ACYDPINLYNSASVVMNLYYQNQGRQYSKCDFEGSGIISVTDP 111
>At2g02610.1 68415.m00200 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 627
Score = 29.5 bits (63), Expect = 2.7
Identities = 16/48 (33%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = -1
Query: 540 VPDDLYHPFSLKIAHNGTLRHSSNRHHIS-NFKSCFLSTINKLACVEP 400
VP++L S + NG + H S+RHH+ + F+ ACV P
Sbjct: 327 VPEELEDVESFRKIANGVILHFSHRHHLKFDISGVFVGNKFCQACVLP 374
>At4g14250.1 68417.m02198 UBX domain-containing protein low
similarity to 60S ribosomal protein L2 [Nicotiana
tabacum] GI:9230281; contains Pfam profile PF00789: UBX
domain
Length = 724
Score = 28.7 bits (61), Expect = 4.7
Identities = 20/55 (36%), Positives = 27/55 (49%)
Frame = +3
Query: 600 VKLPSGAKKVLPSSNRGMVGIVAGGGRIDKPILKAGRAYHKYKVKRNCWPYVRGV 764
+ LP +KK + S R M+G +A G + K K +KRN W VRGV
Sbjct: 382 INLPLDSKKTVLSGCRVMIGQIASSG-----LTK------KLMIKRNMWAKVRGV 425
>At2g16365.2 68415.m06033 F-box family protein contains Pfam:PF00646
F-box domain
Length = 455
Score = 28.7 bits (61), Expect = 4.7
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = -2
Query: 575 VSNHSGEVSRGTCQTTSITHFLSR 504
V H GE S Q+ ++ HFLSR
Sbjct: 271 VDGHQGETSNSATQSMNVEHFLSR 294
>At2g16365.1 68415.m06032 F-box family protein contains Pfam:PF00646
F-box domain
Length = 778
Score = 28.7 bits (61), Expect = 4.7
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = -2
Query: 575 VSNHSGEVSRGTCQTTSITHFLSR 504
V H GE S Q+ ++ HFLSR
Sbjct: 271 VDGHQGETSNSATQSMNVEHFLSR 294
>At1g11060.1 68414.m01267 expressed protein
Length = 930
Score = 28.7 bits (61), Expect = 4.7
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = -1
Query: 510 LKIAHNGTLRHSSNRHHISNFKSCFLSTINKLACVE 403
LKI N T + N++H+ FK C S ++++ E
Sbjct: 422 LKIMENATFLSTDNQNHLLGFKKCLGSHDSRMSFTE 457
>At5g65290.1 68418.m08212 LMBR1 integral membrane family protein
contains Pfam PF04791: LMBR1-like conserved region
Length = 733
Score = 28.3 bits (60), Expect = 6.2
Identities = 8/21 (38%), Positives = 13/21 (61%)
Frame = +2
Query: 668 WRWTY*QTYFESWKGIPQVQG 730
W W+Y T+ +W +P +QG
Sbjct: 74 WSWSYWSTFLLTWAVVPLIQG 94
>At1g33110.1 68414.m04089 MATE efflux family protein similar to
ripening regulated protein DDTFR18 [Lycopersicon
esculentum] GI:12231296; contains Pfam profile PF01554:
Uncharacterized membrane protein family
Length = 494
Score = 28.3 bits (60), Expect = 6.2
Identities = 15/46 (32%), Positives = 21/46 (45%)
Frame = -1
Query: 621 WLQTVALLLYA*HQDCVQSQWRSFQRHVPDDLYHPFSLKIAHNGTL 484
WL +A LL+ C + WR F DL+ F L ++ G L
Sbjct: 227 WLPNIAQLLFVTCGGC-KDTWRGFSMMAFKDLWPVFKLSMSSGGML 271
>At3g27670.1 68416.m03455 expressed protein
Length = 1841
Score = 27.9 bits (59), Expect = 8.2
Identities = 11/38 (28%), Positives = 20/38 (52%)
Frame = -1
Query: 465 HHISNFKSCFLSTINKLACVEPSGAMKSSFLVLNLYGS 352
H + + CF I++L V SGA++ L+ + G+
Sbjct: 1204 HEVDDIIGCFKDLISELLIVRKSGALRKRLLMASCIGA 1241
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,154,691
Number of Sequences: 28952
Number of extensions: 391100
Number of successful extensions: 1056
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1008
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1055
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1785055200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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