BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV30178 (650 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g16640.1 68416.m02127 translationally controlled tumor family... 64 1e-10 At3g05540.1 68416.m00607 translationally controlled tumor family... 49 2e-06 At4g29060.1 68417.m04157 elongation factor Ts family protein sim... 34 0.071 At2g02980.1 68415.m00250 pentatricopeptide (PPR) repeat-containi... 33 0.22 At2g17050.1 68415.m01968 disease resistance protein (TIR-NBS-LRR... 31 0.66 At3g56740.1 68416.m06311 ubiquitin-associated (UBA)/TS-N domain-... 30 1.2 At5g58220.1 68418.m07289 expressed protein 30 1.5 At2g41160.1 68415.m05084 ubiquitin-associated (UBA)/TS-N domain-... 29 2.7 At3g59660.1 68416.m06656 C2 domain-containing protein / GRAM dom... 28 6.2 At5g49030.1 68418.m06067 tRNA synthetase class I (I, L, M and V)... 27 8.2 At4g16150.1 68417.m02450 calmodulin-binding protein similar to a... 27 8.2 >At3g16640.1 68416.m02127 translationally controlled tumor family protein similar to translationally controlled tumor protein GB:AAD10032 from [Hevea brasiliensis] Length = 168 Score = 63.7 bits (148), Expect = 1e-10 Identities = 37/87 (42%), Positives = 53/87 (60%), Gaps = 3/87 (3%) Frame = +1 Query: 1 ITGDEMFSDTYKMKLVDE-VIYEVTGRLVTRAQGDIQIEGFNPSAEEA--DEGTDSAVES 171 +TGDE+ SD++ K ++ +++EV G+ VT D+ I G NPSAEE DEG D + + Sbjct: 8 LTGDELLSDSFPYKEIENGILWEVEGKWVTVGAVDVNI-GANPSAEEGGEDEGVDDSTQK 66 Query: 172 GVDIVLNHRLVETYAFGDKKSYTLYLK 252 VDIV RL E + DKK + Y+K Sbjct: 67 VVDIVDTFRLQEQPTY-DKKGFIAYIK 92 Score = 52.4 bits (120), Expect = 3e-07 Identities = 30/81 (37%), Positives = 44/81 (54%) Frame = +3 Query: 249 QRYMKKLVAKLEEKAPDQVEVFKTNMNKVMKDILGRFKELQFFTGESMDCDGMVAMMEYR 428 ++Y+K L KL E+ DQ VFK + K +L R + QFF GE M D + Y+ Sbjct: 92 KKYIKLLTPKLSEE--DQA-VFKKGIEGATKFLLPRLSDFQFFVGEGMHDDSTLVFAYYK 148 Query: 429 DFDGTQIPIMMFFKHGLEEEK 491 +G+ P ++F HGL+E K Sbjct: 149 --EGSTNPTFLYFAHGLKEVK 167 >At3g05540.1 68416.m00607 translationally controlled tumor family protein similar to translationally controlled tumor protein GB:AAD10032 from [Hevea brasiliensis] Length = 156 Score = 49.2 bits (112), Expect = 2e-06 Identities = 27/82 (32%), Positives = 47/82 (57%), Gaps = 1/82 (1%) Frame = +3 Query: 249 QRYMKKLVAKLEEKAPDQVEVFKTNMNKVMKDILGRFKELQFFTGESMDC-DGMVAMMEY 425 +RY+K+L KL+ + E+FK ++ K ++ + K+ QFF GESM+ +G + Y Sbjct: 79 KRYIKQLSPKLDS---ENQELFKKHIESATKFLMSKLKDFQFFVGESMEGEEGSLVFAYY 135 Query: 426 RDFDGTQIPIMMFFKHGLEEEK 491 R +G P ++ +GL+E K Sbjct: 136 R--EGATDPTFLYLAYGLKEIK 155 Score = 43.6 bits (98), Expect = 1e-04 Identities = 31/86 (36%), Positives = 45/86 (52%), Gaps = 2/86 (2%) Frame = +1 Query: 1 ITGDEMFSDTYKMKLVDEVIYEVTGRLVTRAQGDIQIEGFNPSAEEA--DEGTDSAVESG 174 +TGDE+ SD++ K ++ G L ++EG NPS EE DEG D Sbjct: 8 LTGDELLSDSFPYKEIEN------GMLW-------EVEGKNPSGEEGGEDEGVDDQAVKV 54 Query: 175 VDIVLNHRLVETYAFGDKKSYTLYLK 252 VDI+ RL E +F DKK + +++K Sbjct: 55 VDIIDTFRLQEQPSF-DKKQFVMFMK 79 >At4g29060.1 68417.m04157 elongation factor Ts family protein similar to SP|P35019 Elongation factor Ts (EF-Ts) {Galdieria sulphuraria}; contains Pfam profiles PF00627: UBA/TS-N domain, PF00889: Elongation factor TS, PF00575: S1 RNA binding domain Length = 953 Score = 34.3 bits (75), Expect = 0.071 Identities = 21/58 (36%), Positives = 33/58 (56%), Gaps = 2/58 (3%) Frame = +1 Query: 7 GDEMFSDTYKMKLVDEVIYEVT--GRLVTRAQGDIQIEGFNPSAEEADEGTDSAVESG 174 G+ S K +++D V+ +T G +T +G+ EGF P+AEEAD+G S + G Sbjct: 238 GEGFNSKFAKGQMLDGVVKNLTRSGAFITIGEGE---EGFLPTAEEADDGIGSMMMGG 292 >At2g02980.1 68415.m00250 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 603 Score = 32.7 bits (71), Expect = 0.22 Identities = 19/70 (27%), Positives = 29/70 (41%) Frame = +3 Query: 381 GESMDCDGMVAMMEYRDFDGTQIPIMMFFKHGLEEEKF*MLDKNNSIIPNWRLDTFVFLF 560 G D + M Y+D I+ + HG E+ M ++ S N + D FL Sbjct: 279 GSLDDAVSIFEKMRYKDTQAWSAMIVAYANHGKAEKSMLMFERMRS--ENVQPDEITFLG 336 Query: 561 IYQSCPHPGR 590 + +C H GR Sbjct: 337 LLNACSHTGR 346 >At2g17050.1 68415.m01968 disease resistance protein (TIR-NBS-LRR class), putative domain signature TIR-NBS-LRR exists, suggestive of a disease resistance protein. Length = 1355 Score = 31.1 bits (67), Expect = 0.66 Identities = 18/58 (31%), Positives = 30/58 (51%), Gaps = 1/58 (1%) Frame = +3 Query: 225 QEILHIVPQRYMKKLVAKLEEKAPDQVEVFKTNMNKVMKDILGRFKELQ-FFTGESMD 395 +E+L + M+ + KL P +EVFK+N N + ++ F ++ FF GE D Sbjct: 217 EEMLSHMKSDKMEVKLLKLNHPPPQIMEVFKSNYNALNENEKSMFLDIACFFRGEKAD 274 >At3g56740.1 68416.m06311 ubiquitin-associated (UBA)/TS-N domain-containing protein contains Pfam profile PF00627: UBA/TS-N domain Length = 293 Score = 30.3 bits (65), Expect = 1.2 Identities = 18/51 (35%), Positives = 25/51 (49%) Frame = -2 Query: 343 SFITLFMFVLNTSTWSGAFSSNFATNFFIYL*GTMCRISCHRRRMFRLACG 191 SFI ++ + ST F NF+ FIYL G +S +R +F CG Sbjct: 129 SFIPFYLDI-PVSTRFRVFGVNFSDKSFIYLAGVQLLLSSWKRSIFPGICG 178 >At5g58220.1 68418.m07289 expressed protein Length = 324 Score = 29.9 bits (64), Expect = 1.5 Identities = 12/39 (30%), Positives = 24/39 (61%) Frame = +3 Query: 228 EILHIVPQRYMKKLVAKLEEKAPDQVEVFKTNMNKVMKD 344 E+LH + +RY + + +LE A +Q+++ + M K+ D Sbjct: 122 EMLHALKERYENRPIVELEIAAMEQMKITELRMAKLFSD 160 >At2g41160.1 68415.m05084 ubiquitin-associated (UBA)/TS-N domain-containing protein contains Pfam profile PF00627: UBA/TS-N domain Length = 287 Score = 29.1 bits (62), Expect = 2.7 Identities = 16/51 (31%), Positives = 25/51 (49%) Frame = -2 Query: 343 SFITLFMFVLNTSTWSGAFSSNFATNFFIYL*GTMCRISCHRRRMFRLACG 191 SF+ F+ + T + G +F+ FIYL G +S +R +F CG Sbjct: 129 SFVPFFLDIPVTKRF-GVLGVHFSDKSFIYLAGVQLLLSSWKRSIFTGICG 178 >At3g59660.1 68416.m06656 C2 domain-containing protein / GRAM domain-containing protein low similarity to GLUT4 vesicle protein [Rattus norvegicus] GI:4193489; contains Pfam profiles PF00168: C2 domain, PF02893: GRAM domain Length = 594 Score = 27.9 bits (59), Expect = 6.2 Identities = 11/30 (36%), Positives = 17/30 (56%), Gaps = 1/30 (3%) Frame = -3 Query: 447 FAYHQSLYIPSWQPC-HHNPWTPQ*RTEVP 361 F YH +Y+ +W C H N ++ Q + VP Sbjct: 255 FLYHGRMYVSAWHICFHSNVFSKQMKVVVP 284 >At5g49030.1 68418.m06067 tRNA synthetase class I (I, L, M and V) family protein similar to SP|P41972 Isoleucyl-tRNA synthetase (EC 6.1.1.5) (Isoleucine--tRNA ligase) (IleRS) {Staphylococcus aureus}; contains Pfam profile PF00133: tRNA synthetases class I (I, L, M and V) Length = 1093 Score = 27.5 bits (58), Expect = 8.2 Identities = 12/31 (38%), Positives = 20/31 (64%), Gaps = 3/31 (9%) Frame = +3 Query: 324 MNKVMKDILGRFKELQFFTGE---SMDCDGM 407 +NK++KDI+ R+K LQ + + DC G+ Sbjct: 167 LNKILKDIINRYKLLQNYKVQYVPGWDCHGL 197 >At4g16150.1 68417.m02450 calmodulin-binding protein similar to anther ethylene-upregulated calmodulin-binding protein ER1 GI:11612392 from [Nicotiana tabacum] Length = 906 Score = 27.5 bits (58), Expect = 8.2 Identities = 11/16 (68%), Positives = 12/16 (75%) Frame = +2 Query: 464 FQTWSRRREILNVR*K 511 FQTW RRE LN+R K Sbjct: 770 FQTWKMRREFLNMRKK 785 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,478,308 Number of Sequences: 28952 Number of extensions: 262363 Number of successful extensions: 743 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 723 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 741 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1354097952 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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