Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30171
(712 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 120 3e-28
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 117 1e-27
SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces pomb... 30 0.28
SPAC17H9.06c |||conserved fungal protein|Schizosaccharomyces pom... 29 0.66
SPAC5H10.09c |||3-methyl-2-oxobutanoatehydroxymethyltransferase|... 27 2.6
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 27 3.5
SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces p... 27 3.5
SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst... 27 3.5
SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi... 26 4.6
SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 26 6.1
SPAC1420.01c ||SPAC56E4.08c|DUF1752 family protein|Schizosacchar... 25 8.1
SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosacc... 25 8.1
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 120 bits (288), Expect = 3e-28
Identities = 55/84 (65%), Positives = 69/84 (82%), Gaps = 2/84 (2%)
Frame = +2
Query: 254 DVVGARRSSWRVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIP 427
+++GARR+SWR+ISSIEQK E G+ R+ + KEYR K+E EL +IC+DVL +L+KHLIP
Sbjct: 52 NIIGARRASWRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIP 111
Query: 428 KASNPESKVFYLKMKGDYYRYLAE 499
A+ ESKVFY KMKGDYYRYLAE
Sbjct: 112 AATTGESKVFYYKMKGDYYRYLAE 135
Score = 60.5 bits (140), Expect = 2e-10
Identities = 28/50 (56%), Positives = 37/50 (74%)
Frame = +1
Query: 511 ETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNSP 660
E + S +AY+ A +I+ A++ PT P+RLGLALNFSVFYYEIL+SP
Sbjct: 140 EVCKEAADSSLEAYKAASDIAVAELPPTDPMRLGLALNFSVFYYEILDSP 189
Score = 58.0 bits (134), Expect = 1e-09
Identities = 31/51 (60%), Positives = 37/51 (72%)
Frame = +3
Query: 102 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYK 254
MS +E V AKLAEQAERY++M MK+V + +LS EERNLLSVAYK
Sbjct: 1 MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERNLLSVAYK 51
Score = 29.5 bits (63), Expect = 0.50
Identities = 14/38 (36%), Positives = 20/38 (52%)
Frame = +3
Query: 597 PHKAGSGVKFLSLLL*DIKFADKACQLAKQAFDDAIAE 710
P + G + F + + AC LAKQ FD+AI+E
Sbjct: 169 PMRLGLALNFSVFYYEILDSPESACHLAKQVFDEAISE 206
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 117 bits (282), Expect = 1e-27
Identities = 54/86 (62%), Positives = 70/86 (81%), Gaps = 2/86 (2%)
Frame = +2
Query: 254 DVVGARRSSWRVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIP 427
+V+GARR+SWR++SSIEQK E G+ + ++ KEYR K+E+EL IC D+L +L+KHLIP
Sbjct: 53 NVIGARRASWRIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIP 112
Query: 428 KASNPESKVFYLKMKGDYYRYLAEVA 505
A++ ESKVFY KMKGDYYRYLAE A
Sbjct: 113 NAASAESKVFYYKMKGDYYRYLAEFA 138
Score = 70.9 bits (166), Expect = 2e-13
Identities = 33/52 (63%), Positives = 39/52 (75%)
Frame = +1
Query: 511 ETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNSPTR 666
E R + S + Y+ A EI+ A++ PTHPIRLGLALNFSVFYYEILNSP R
Sbjct: 141 EKRQHSADQSLEGYKAASEIATAELAPTHPIRLGLALNFSVFYYEILNSPDR 192
Score = 59.3 bits (137), Expect = 5e-10
Identities = 30/50 (60%), Positives = 35/50 (70%)
Frame = +3
Query: 105 SVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYK 254
+ +E+ V AKLAEQAERY+ M MK V T EL+ EERNLLSVAYK
Sbjct: 3 TTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYK 52
Score = 33.5 bits (73), Expect = 0.030
Identities = 18/44 (40%), Positives = 24/44 (54%)
Frame = +3
Query: 579 ENAAHTPHKAGSGVKFLSLLL*DIKFADKACQLAKQAFDDAIAE 710
E A P + G + F + D+AC LAKQAFD+AI+E
Sbjct: 164 ELAPTHPIRLGLALNFSVFYYEILNSPDRACYLAKQAFDEAISE 207
>SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 605
Score = 30.3 bits (65), Expect = 0.28
Identities = 16/38 (42%), Positives = 23/38 (60%)
Frame = -3
Query: 563 KASWYAFCESSTTECLVSLWPLLQGTCSNLLSF*GKTL 450
+A W AF E+S +E ++L +L S+LLSF TL
Sbjct: 64 EAVWAAFSEASCSERKLALQGILNNCSSSLLSFASSTL 101
>SPAC17H9.06c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 601
Score = 29.1 bits (62), Expect = 0.66
Identities = 26/91 (28%), Positives = 42/91 (46%), Gaps = 9/91 (9%)
Frame = +2
Query: 284 RVISSIEQKTEGSERKQQMAKEYRVKVEKE-LREICYDVLGL--LDKHL---IPKASNPE 445
+V+SS + E S + + R E E L E+ +V L +D+ +P+ NP
Sbjct: 263 KVLSSSSEDDESSSPEDLLKPIIRSTEEMENLNELEQEVQDLDPIDEGFEEKVPRFRNPS 322
Query: 446 SKVFYLK---MKGDYYRYLAEVATEKPDILL 529
K FY K ++ Y L + +EK D L+
Sbjct: 323 KKAFYEKLHSLRNRSYSKLESLTSEKSDTLI 353
>SPAC5H10.09c
|||3-methyl-2-
oxobutanoatehydroxymethyltransferase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 267
Score = 27.1 bits (57), Expect = 2.6
Identities = 13/35 (37%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +3
Query: 471 KEITTGTLQKWPQRNQTFCC-RGFTESIPRCF*NQ 572
K+IT TL++W N+ F C + S R F Q
Sbjct: 4 KQITISTLRQWKLANKKFACITAYDASFSRLFAEQ 38
>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1207
Score = 26.6 bits (56), Expect = 3.5
Identities = 15/41 (36%), Positives = 24/41 (58%), Gaps = 1/41 (2%)
Frame = +1
Query: 511 ETRHSVVEDSQKAYQDAFEISKAKMQPTHP-IRLGLALNFS 630
E R ED+++A + ++ + A +QP H I L LA+N S
Sbjct: 1059 ERRRLGEEDTKRALELSYYFASADLQPMHSIIALRLAINAS 1099
>SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 355
Score = 26.6 bits (56), Expect = 3.5
Identities = 21/69 (30%), Positives = 37/69 (53%), Gaps = 2/69 (2%)
Frame = +2
Query: 302 EQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKM-KGD 478
E E+ Q++A+ Y+V + +LRE YD LG + +P A ++ F+ + GD
Sbjct: 43 ENPEAAREKFQKLAEAYQVLSDPKLRE-KYDKLGKVG--AVPDAGFEDAFEFFKNLFGGD 99
Query: 479 YYR-YLAEV 502
+R Y+ E+
Sbjct: 100 SFRDYVGEL 108
>SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit
Pst2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1075
Score = 26.6 bits (56), Expect = 3.5
Identities = 12/36 (33%), Positives = 19/36 (52%), Gaps = 1/36 (2%)
Frame = +1
Query: 598 PIRLGLALN-FSVFYYEILNSPTRRVNSPNRLSTMP 702
P+ GL + FS+F ++ P VN+PN + P
Sbjct: 600 PLHFGLEKDKFSIFLDQVFRGPDYDVNAPNIVGNKP 635
>SPBC839.10 |usp107|snu71|U1 snRNP-associated protein
Usp107|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 26.2 bits (55), Expect = 4.6
Identities = 13/42 (30%), Positives = 22/42 (52%)
Frame = +2
Query: 305 QKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 430
+K + + KEY K +++LR+ D+ LL KH I +
Sbjct: 328 EKQRSKDEYASLYKEYTRKEQEKLRKQNDDLQNLLSKHRISR 369
>SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1067
Score = 25.8 bits (54), Expect = 6.1
Identities = 16/46 (34%), Positives = 21/46 (45%)
Frame = +1
Query: 7 EKLILCLNLLVQIHFSPSDKGISELVLFHRPRCPSTRKNWCNVPNW 144
EKL+ C HF +G+ + L RC +T CNVP W
Sbjct: 683 EKLVACTT-----HFY---EGVRKSALSSLWRCATTYYKVCNVPQW 720
>SPAC1420.01c ||SPAC56E4.08c|DUF1752 family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 580
Score = 25.4 bits (53), Expect = 8.1
Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
Frame = +1
Query: 49 FSPSDK-GISELVLFHRPRCPSTRKNWCNVPNWPNKLSDMTT 171
FSP +K + +L LFH + PS+++ V N + SD +T
Sbjct: 162 FSPPEKPSMKDLALFHGNKSPSSKETIPKVSN--SNSSDTST 201
>SPBC6B1.04 |mde4||monopolin-like complex subunit
Mde4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 421
Score = 25.4 bits (53), Expect = 8.1
Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 2/43 (4%)
Frame = +2
Query: 323 ERKQQMAKEYRVKVEKELREICYDV--LGLLDKHLIPKASNPE 445
E++Q A +YR+KVE+ +I V + L+ L + SNPE
Sbjct: 85 EQEQNEANDYRLKVERLEHKISDYVQEINSLNSQLQIQKSNPE 127
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,980,620
Number of Sequences: 5004
Number of extensions: 60357
Number of successful extensions: 204
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 191
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 202
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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