Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30171
(712 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.) 120 9e-28
SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.) 101 6e-22
SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0) 89 4e-18
SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44) 76 2e-14
SB_34218| Best HMM Match : No HMM Matches (HMM E-Value=.) 55 7e-08
SB_42896| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.6
SB_24162| Best HMM Match : RVT_1 (HMM E-Value=0) 30 2.1
SB_13504| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.8
SB_15350| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.9
SB_11347| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.9
SB_45140| Best HMM Match : RVT_1 (HMM E-Value=3.8e-32) 28 6.5
SB_12646| Best HMM Match : SSrecog (HMM E-Value=0) 28 6.5
>SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 309
Score = 120 bits (290), Expect = 9e-28
Identities = 58/84 (69%), Positives = 66/84 (78%)
Frame = +2
Query: 254 DVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKA 433
+VVGA+RSSWRVISSIEQK EGSERK+Q + YR +E EL E+C VL LL+ LIP A
Sbjct: 29 NVVGAKRSSWRVISSIEQKLEGSERKKQNTETYRQTIENELNEVCETVLKLLESKLIPNA 88
Query: 434 SNPESKVFYLKMKGDYYRYLAEVA 505
+ ESKVFYLKMKGDYYRY EVA
Sbjct: 89 QSTESKVFYLKMKGDYYRYEGEVA 112
Score = 59.3 bits (137), Expect = 3e-09
Identities = 29/49 (59%), Positives = 37/49 (75%), Gaps = 2/49 (4%)
Frame = +1
Query: 511 ETRHSVVEDSQKAYQDAFEISKA--KMQPTHPIRLGLALNFSVFYYEIL 651
+ R VV+ + KAY +A EI++ K+ PT PIRLGLALNFSVFYYEI+
Sbjct: 115 DRRREVVQKAMKAYSEAQEIAEKDPKLPPTDPIRLGLALNFSVFYYEIV 163
Score = 51.6 bits (118), Expect = 6e-07
Identities = 26/45 (57%), Positives = 35/45 (77%), Gaps = 3/45 (6%)
Frame = +1
Query: 523 SVVEDSQKAYQDAFEISKA---KMQPTHPIRLGLALNFSVFYYEI 648
+VVE + +AY++A E ++ K+ PT PIRLGLALNFSVF+YEI
Sbjct: 207 TVVEKALQAYKEAKEAAETGDGKLAPTDPIRLGLALNFSVFHYEI 251
Score = 40.3 bits (90), Expect = 0.002
Identities = 20/28 (71%), Positives = 20/28 (71%)
Frame = +3
Query: 171 MAAAMKEVTETGVELSNEERNLLSVAYK 254
MA AMKE TE L EERNLLSVAYK
Sbjct: 1 MAKAMKEATEISETLEQEERNLLSVAYK 28
Score = 33.9 bits (74), Expect = 0.13
Identities = 14/17 (82%), Positives = 17/17 (100%)
Frame = +3
Query: 660 DKACQLAKQAFDDAIAE 710
++AC+LAKQAFDDAIAE
Sbjct: 256 EEACKLAKQAFDDAIAE 272
Score = 32.7 bits (71), Expect = 0.30
Identities = 16/38 (42%), Positives = 23/38 (60%)
Frame = +3
Query: 597 PHKAGSGVKFLSLLL*DIKFADKACQLAKQAFDDAIAE 710
P + G + F ++ + +AC LAK+AFDDAIAE
Sbjct: 146 PIRLGLALNFSVFYYEIVEDSKQACDLAKKAFDDAIAE 183
>SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 219
Score = 101 bits (242), Expect = 6e-22
Identities = 48/82 (58%), Positives = 65/82 (79%)
Frame = +2
Query: 254 DVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKA 433
++VG RRSSWRVISSIE+KT S + K+Y+ +EKEL+++C +VLG+L++ LIP A
Sbjct: 131 NIVGQRRSSWRVISSIEEKTAESS-SLAIVKKYKACIEKELKDLCKEVLGILER-LIPGA 188
Query: 434 SNPESKVFYLKMKGDYYRYLAE 499
+ E+KVFY K+KGDYYRYLAE
Sbjct: 189 EDEENKVFYFKLKGDYYRYLAE 210
Score = 63.7 bits (148), Expect = 1e-10
Identities = 31/51 (60%), Positives = 39/51 (76%)
Frame = +3
Query: 102 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYK 254
M V +E L+ AKL+EQ +RYD+MA MKEV+E +LS EERNLLSV+YK
Sbjct: 80 MMVVRETLIYNAKLSEQCDRYDEMAKIMKEVSEKYPKLSKEERNLLSVSYK 130
>SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0)
Length = 248
Score = 88.6 bits (210), Expect = 4e-18
Identities = 46/87 (52%), Positives = 59/87 (67%), Gaps = 3/87 (3%)
Frame = +2
Query: 254 DVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKA 433
+VVGARRSSWRVISS+EQK E + K+YR + EL C +VL +L+ +L+
Sbjct: 58 NVVGARRSSWRVISSMEQKAP--EEMAALTKKYREDITNELNGKCAEVLDILENYLLKDG 115
Query: 434 S---NPESKVFYLKMKGDYYRYLAEVA 505
N E+KVFYLKM+GDY+RYL EVA
Sbjct: 116 QDDINTEAKVFYLKMRGDYHRYLVEVA 142
Score = 68.9 bits (161), Expect = 4e-12
Identities = 33/55 (60%), Positives = 40/55 (72%)
Frame = +3
Query: 90 PSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYK 254
P+ +EEL+ AK+AEQAERYDDM AM VT+ G L++EERNLLSVAYK
Sbjct: 3 PNFVSKCSREELIHLAKMAEQAERYDDMVNAMSAVTKEGKPLNDEERNLLSVAYK 57
Score = 60.9 bits (141), Expect = 1e-09
Identities = 28/50 (56%), Positives = 37/50 (74%)
Frame = +1
Query: 511 ETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNSP 660
++R +E S++AY+DA ++ P+HPIRLGLALNFSVFYYEI N P
Sbjct: 145 DSRKENIEKSREAYKDA-SAKAEELSPSHPIRLGLALNFSVFYYEIENKP 193
Score = 29.9 bits (64), Expect = 2.1
Identities = 12/15 (80%), Positives = 15/15 (100%)
Frame = +3
Query: 663 KACQLAKQAFDDAIA 707
+AC+LAK+AFDDAIA
Sbjct: 195 EACKLAKEAFDDAIA 209
>SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44)
Length = 251
Score = 76.2 bits (179), Expect = 2e-14
Identities = 37/75 (49%), Positives = 52/75 (69%), Gaps = 3/75 (4%)
Frame = +2
Query: 254 DVVGARRSSWRVISSIEQKTEGSER---KQQMAKEYRVKVEKELREICYDVLGLLDKHLI 424
+V+GARR+SWR+I+SIEQK E K +M + YR +E+EL+ IC ++L LLD LI
Sbjct: 51 NVIGARRASWRIITSIEQKEESKGEDMAKLEMIRNYRKTIEEELKTICGEILSLLDDSLI 110
Query: 425 PKASNPESKVFYLKM 469
+ + ESKVFY K+
Sbjct: 111 KNSQSEESKVFYNKI 125
Score = 73.3 bits (172), Expect = 2e-13
Identities = 36/48 (75%), Positives = 39/48 (81%)
Frame = +3
Query: 111 DKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYK 254
DKEE V AKLAEQAERYDDM +MKEV + G ELS E+RNLLSVAYK
Sbjct: 3 DKEEHVYMAKLAEQAERYDDMVNSMKEVAKMGTELSTEDRNLLSVAYK 50
>SB_34218| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 607
Score = 54.8 bits (126), Expect = 7e-08
Identities = 27/51 (52%), Positives = 33/51 (64%)
Frame = +3
Query: 102 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYK 254
M + ELVQ AKLAEQ ER++D+ MK+ E L+ E RNLLSV YK
Sbjct: 186 MQDSRNELVQLAKLAEQTERFEDVILYMKKAIEINPSLNKEHRNLLSVGYK 236
>SB_42896| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 994
Score = 30.3 bits (65), Expect = 1.6
Identities = 16/51 (31%), Positives = 28/51 (54%)
Frame = +3
Query: 78 ISPLPSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEER 230
+SPLPS+ +E+V KLAE+ E ++++ T+ E + EE+
Sbjct: 643 VSPLPSTATEDQMQEVVDSNKLAEKKEVTEEVSPVKPLSTKESKENALEEK 693
>SB_24162| Best HMM Match : RVT_1 (HMM E-Value=0)
Length = 801
Score = 29.9 bits (64), Expect = 2.1
Identities = 20/74 (27%), Positives = 35/74 (47%)
Frame = +3
Query: 90 PSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKMS*VP 269
P + +++K E++QR Y ++ + G E S E+R L+ A+ +
Sbjct: 694 PYTQCNINKIEMIQRRAARFVFNDYSRYSSVSPMINSLGWE-SLEQRRQLNQAHMFYKIY 752
Query: 270 DGHHGVSFPQLNRK 311
+G G+SFP N K
Sbjct: 753 NGLVGISFPPENLK 766
>SB_13504| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 4924
Score = 29.5 bits (63), Expect = 2.8
Identities = 20/59 (33%), Positives = 33/59 (55%)
Frame = +1
Query: 502 GHRETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNSPTRRVNS 678
G + S+V D+ +A +D KA+ Q + ALN S+F++E++NS + VNS
Sbjct: 4022 GETRRKRSLV-DTPEANED-----KAQHQVPDAEKCQFALNASLFFFEVINSLSSVVNS 4074
>SB_15350| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1126
Score = 28.7 bits (61), Expect = 4.9
Identities = 27/105 (25%), Positives = 46/105 (43%), Gaps = 2/105 (1%)
Frame = +2
Query: 284 RVISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYL 463
R++ IE+K E E ++ AKE + + E+E + IC L+ + + E + L
Sbjct: 919 RIMKEIEEK-EKKEEAERKAKEEKEREERERKRIC---------QLLKEKAEQERREREL 968
Query: 464 KMKGDYYRYLAE--VATEKPDILL*RIHRKHTKMLLKSARRKCSP 592
K + + R E + E+ L + RK + LK K P
Sbjct: 969 KAREEKLRLAREQMMREERERTLKLELERKRMEERLKDQASKNDP 1013
>SB_11347| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1234
Score = 28.7 bits (61), Expect = 4.9
Identities = 16/56 (28%), Positives = 30/56 (53%)
Frame = +2
Query: 272 RSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASN 439
+ W ++S E+ +ER++Q E +VK E++ R+ ++ + HL K SN
Sbjct: 338 KHQWNLLSEDEKNKIKAERRKQKRLELKVKREEKERKRLEELKRVSFAHLGMKLSN 393
>SB_45140| Best HMM Match : RVT_1 (HMM E-Value=3.8e-32)
Length = 868
Score = 28.3 bits (60), Expect = 6.5
Identities = 12/42 (28%), Positives = 23/42 (54%), Gaps = 2/42 (4%)
Frame = -1
Query: 556 LGMLSVNPLQQNVWFLCGHFCKVPVVI--SFHFEVKHFTFWI 437
L +LS +P++ W+L CK ++ +FE+KH + +
Sbjct: 781 LKLLSEDPIEVQYWYLEWTICKTRNIMLRDLNFEIKHIDYML 822
>SB_12646| Best HMM Match : SSrecog (HMM E-Value=0)
Length = 783
Score = 28.3 bits (60), Expect = 6.5
Identities = 11/34 (32%), Positives = 22/34 (64%)
Frame = +2
Query: 329 KQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 430
K++M ++Y K+EKE+ Y+++ L K ++ K
Sbjct: 292 KEEMKEKYDGKIEKEMSGAIYEIISRLMKAVVGK 325
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,611,275
Number of Sequences: 59808
Number of extensions: 460615
Number of successful extensions: 1332
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1210
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1328
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1877743452
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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