Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30170
(731 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 86 2e-17
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 85 3e-17
At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1 (e... 76 2e-14
At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2 (e... 74 1e-13
At2g40660.1 68415.m05017 tRNA-binding region domain-containing p... 35 0.064
At4g26090.1 68417.m03756 disease resistance protein RPS2 (CC-NBS... 29 3.2
At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 28 5.6
At5g18830.2 68418.m02238 squamosa promoter-binding protein-like ... 28 7.3
At5g18830.1 68418.m02237 squamosa promoter-binding protein-like ... 28 7.3
At1g68210.1 68414.m07792 two-component responsive regulator fami... 28 7.3
At2g36190.1 68415.m04442 beta-fructosidase, putative / beta-fruc... 27 9.7
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 86.2 bits (204), Expect = 2e-17
Identities = 40/65 (61%), Positives = 50/65 (76%), Gaps = 3/65 (4%)
Frame = +1
Query: 511 TIEMEGLLWGASKLVPVGYGINKLQIMCVIEDDKVSVDLLTEK---IQEFEDFVQSVDIA 681
+I+MEGL WGASKLVPVGYGI KLQI+C I DD VS+D + E+ ++ ++VQS DI
Sbjct: 167 SIQMEGLFWGASKLVPVGYGIKKLQILCTIVDDLVSIDTMIEEQLTVEPINEYVQSCDIV 226
Query: 682 AFNKI 696
AFNKI
Sbjct: 227 AFNKI 231
Score = 48.4 bits (110), Expect = 5e-06
Identities = 20/58 (34%), Positives = 34/58 (58%)
Frame = +3
Query: 36 AVGDVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQI 209
A ++ + GL L+++L +SY++GY S+ D+ VF + K P + + RWYN I
Sbjct: 3 AFPNLNSDAGLKKLDEHLLTRSYITGYQASKDDITVFAALAKPPTSQYVNASRWYNHI 60
Score = 48.4 bits (110), Expect = 5e-06
Identities = 21/39 (53%), Positives = 28/39 (71%)
Frame = +2
Query: 392 KAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQV 508
+A + K S K KSS+L+D+KPWDDETDMK++E V
Sbjct: 127 RAASVKASTKKKESGKSSVLIDIKPWDDETDMKKLEEAV 165
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 85.4 bits (202), Expect = 3e-17
Identities = 40/65 (61%), Positives = 49/65 (75%), Gaps = 3/65 (4%)
Frame = +1
Query: 511 TIEMEGLLWGASKLVPVGYGINKLQIMCVIEDDKVSVDLLTEK---IQEFEDFVQSVDIA 681
+I+MEGL WGASKLVPVGYGI KL IMC I DD VS+D + E+ ++ ++VQS DI
Sbjct: 167 SIQMEGLFWGASKLVPVGYGIKKLHIMCTIVDDLVSIDTMIEEQLTVEPINEYVQSCDIV 226
Query: 682 AFNKI 696
AFNKI
Sbjct: 227 AFNKI 231
Score = 50.0 bits (114), Expect = 2e-06
Identities = 20/58 (34%), Positives = 35/58 (60%)
Frame = +3
Query: 36 AVGDVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQI 209
A ++ + GL L+++L +SY++GY S+ D+ VF + K P + +V RW+N I
Sbjct: 3 AFPNLNSGSGLKKLDEHLLTRSYITGYQASKDDITVFTALSKPPTSEFVNVSRWFNHI 60
Score = 48.4 bits (110), Expect = 5e-06
Identities = 21/39 (53%), Positives = 28/39 (71%)
Frame = +2
Query: 392 KAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQV 508
+A + K S K KSS+L+D+KPWDDETDMK++E V
Sbjct: 127 RAASVKASTKKKESGKSSVLMDIKPWDDETDMKKLEEAV 165
>At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1
(eEF1Balpha1) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686819
Length = 228
Score = 76.2 bits (179), Expect = 2e-14
Identities = 40/65 (61%), Positives = 46/65 (70%), Gaps = 3/65 (4%)
Frame = +1
Query: 511 TIEMEGLLWGASKLVPVGYGINKLQIMCVIEDDKVSVDLLTEKIQEFE---DFVQSVDIA 681
+++M GL WGASKLVPVGYGI KL IM I DD VSVD L E E +++QSVDI
Sbjct: 164 SVQMPGLTWGASKLVPVGYGIKKLTIMMTIVDDLVSVDNLIEDHLTSEPNNEYIQSVDIV 223
Query: 682 AFNKI 696
AFNKI
Sbjct: 224 AFNKI 228
Score = 49.6 bits (113), Expect = 2e-06
Identities = 22/58 (37%), Positives = 36/58 (62%)
Frame = +3
Query: 45 DVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIASY 218
D+ T +GL L ++LA K+Y+SG S DV+V+ V + P P+ +WY+ +AS+
Sbjct: 7 DLHTERGLKTLEEHLAGKTYISGDQLSVDDVKVYAAVLENPGDGFPNASKWYDSVASH 64
Score = 49.6 bits (113), Expect = 2e-06
Identities = 22/36 (61%), Positives = 27/36 (75%)
Frame = +2
Query: 401 ADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQV 508
A K +KK KSS+LL+VKPWDDETDMK++E V
Sbjct: 127 AKKDTKKTKESGKSSVLLEVKPWDDETDMKKLEEAV 162
>At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2
(eEF1Balpha2) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686821
Length = 224
Score = 73.7 bits (173), Expect = 1e-13
Identities = 39/64 (60%), Positives = 43/64 (67%), Gaps = 3/64 (4%)
Frame = +1
Query: 514 IEMEGLLWGASKLVPVGYGINKLQIMCVIEDDKVSVDLLTEKIQEFE---DFVQSVDIAA 684
+EM GL WGASKLVPVGYGI KL IM I DD VS D L E E +++QS DI A
Sbjct: 161 VEMPGLFWGASKLVPVGYGIKKLTIMFTIVDDLVSPDNLIEDFLTSEPNNEYIQSCDIVA 220
Query: 685 FNKI 696
FNKI
Sbjct: 221 FNKI 224
Score = 53.6 bits (123), Expect = 1e-07
Identities = 23/36 (63%), Positives = 28/36 (77%)
Frame = +2
Query: 401 ADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQV 508
A K +KKP KSS+L+DVKPWDDETDMK++E V
Sbjct: 123 AKKDTKKPKESGKSSVLMDVKPWDDETDMKKLEEAV 158
Score = 46.4 bits (105), Expect = 2e-05
Identities = 20/57 (35%), Positives = 34/57 (59%)
Frame = +3
Query: 45 DVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIAS 215
D+ T +G+ + ++LA K+Y+SG S DV+V+ V P+ P+ +WY +AS
Sbjct: 7 DLHTEEGVKSVEEHLAGKTYISGDQLSVDDVKVYAAVPVKPSDAFPNASKWYESVAS 63
>At2g40660.1 68415.m05017 tRNA-binding region domain-containing
protein similar to SP|Q12904 Multisynthetase complex
auxiliary component p43 [Contains: Endothelial-monocyte
activating polypeptide II (EMAP-II) (Small inducible
cytokine subfamily E member 1)] {Homo sapiens}; contains
Pfam profile PF01588: Putative tRNA binding domain
Length = 389
Score = 34.7 bits (76), Expect = 0.064
Identities = 23/57 (40%), Positives = 29/57 (50%), Gaps = 9/57 (15%)
Frame = +3
Query: 66 LNDLNQYLAEKSYV--SGYTPSQADVQVFEQV-------GKAPAANLPHVLRWYNQI 209
L LN LA KS + +G TPS ADV VF + + +PHV+RW N I
Sbjct: 76 LEKLNLELATKSVLLGNGLTPSAADVAVFSALHSSVLGLSDSDKEKVPHVIRWVNYI 132
>At4g26090.1 68417.m03756 disease resistance protein RPS2
(CC-NBS-LRR class), putative domain signature CC-NBS-LRR
exists, suggestive of a disease resistance protein.
identical to RPS2 (gi:13661831)
Length = 909
Score = 29.1 bits (62), Expect = 3.2
Identities = 8/22 (36%), Positives = 15/22 (68%)
Frame = -3
Query: 723 KYITCYGCLDFVKCSNVNRLYK 658
+Y++C+GC D+ C V+ + K
Sbjct: 103 RYLSCFGCADYKLCKKVSAILK 124
>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
/ VPS11 family protein similar to Vacuolar protein
sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
sapiens]; similar to Vacuolar biogenesis protein END1
(PEP5 protein) (Vacuolar protein sorting 11)
(Swiss-Prot:P12868) [Saccharomyces cerevisiae]
Length = 932
Score = 28.3 bits (60), Expect = 5.6
Identities = 16/32 (50%), Positives = 17/32 (53%)
Frame = +3
Query: 66 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGK 161
L DL Y YVS PSQA V + EQ GK
Sbjct: 495 LEDLGNYDEALQYVSSLEPSQAGVTI-EQYGK 525
>At5g18830.2 68418.m02238 squamosa promoter-binding protein-like 7
(SPL7) identical to squamosa promoter binding
protein-like 7 [Arabidopsis thaliana] GI:5931635;
contains Pfam profile PF03110: SBP domain
Length = 775
Score = 27.9 bits (59), Expect = 7.3
Identities = 18/58 (31%), Positives = 26/58 (44%)
Frame = +3
Query: 120 PSQADVQVFEQVGKAPAANLPHVLRWYNQIASYTSAERKTWSQGTAH*PPVLNPRLPP 293
P + D+Q V P+ + W + + + ER Q H PPVL+P LPP
Sbjct: 10 PPEMDIQPPALVNDDPSTYSSALWDW-GDLLDFAADERLLVDQ--IHFPPVLSPPLPP 64
>At5g18830.1 68418.m02237 squamosa promoter-binding protein-like 7
(SPL7) identical to squamosa promoter binding
protein-like 7 [Arabidopsis thaliana] GI:5931635;
contains Pfam profile PF03110: SBP domain
Length = 801
Score = 27.9 bits (59), Expect = 7.3
Identities = 18/58 (31%), Positives = 26/58 (44%)
Frame = +3
Query: 120 PSQADVQVFEQVGKAPAANLPHVLRWYNQIASYTSAERKTWSQGTAH*PPVLNPRLPP 293
P + D+Q V P+ + W + + + ER Q H PPVL+P LPP
Sbjct: 10 PPEMDIQPPALVNDDPSTYSSALWDW-GDLLDFAADERLLVDQ--IHFPPVLSPPLPP 64
>At1g68210.1 68414.m07792 two-component responsive regulator family
protein / response regulator family protein contains
Pfam profile: PF00072 response regulator receiver domain
Length = 663
Score = 27.9 bits (59), Expect = 7.3
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +1
Query: 595 VIEDDKVSVDLLTEKIQEFEDFVQSVDIAA 684
+I+ D S+ LT +Q+F V SVD+A+
Sbjct: 17 LIDHDTASIASLTSMLQQFSKRVMSVDVAS 46
>At2g36190.1 68415.m04442 beta-fructosidase, putative /
beta-fructofuranosidase, putative similar to
beta-fructofuranosidase GI:18324 from [Daucus carota]
Length = 591
Score = 27.5 bits (58), Expect = 9.7
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +3
Query: 66 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANL 179
+N+ + + ++ V G TP+QADV+V VG A +
Sbjct: 386 MNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEKAEI 423
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,304,306
Number of Sequences: 28952
Number of extensions: 287818
Number of successful extensions: 813
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 786
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 808
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1604469728
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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