BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV30167 (690 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g74050.1 68414.m08576 60S ribosomal protein L6 (RPL6C) simila... 54 1e-07 At1g18540.1 68414.m02313 60S ribosomal protein L6 (RPL6A) simila... 54 1e-07 At1g74060.1 68414.m08578 60S ribosomal protein L6 (RPL6B) simila... 53 2e-07 At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DN... 33 0.18 At5g52730.1 68418.m06545 heavy-metal-associated domain-containin... 31 0.95 At1g78120.1 68414.m09104 tetratricopeptide repeat (TPR)-containi... 30 1.3 At1g13810.1 68414.m01621 expressed protein ; expression supporte... 30 1.3 At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 30 1.7 At3g10440.1 68416.m01252 hypothetical protein 29 2.2 At5g35338.2 68418.m04194 methyl-CpG-binding domain-containing pr... 29 3.8 At5g64030.1 68418.m08039 dehydration-responsive protein-related ... 28 6.7 At5g53930.1 68418.m06710 expressed protein 28 6.7 At1g20540.1 68414.m02559 transducin family protein / WD-40 repea... 28 6.7 At4g23130.2 68417.m03334 receptor-like protein kinase 6 (RLK6) i... 27 8.9 At4g23130.1 68417.m03333 receptor-like protein kinase 6 (RLK6) i... 27 8.9 At3g08670.1 68416.m01007 expressed protein 27 8.9 At2g31270.1 68415.m03818 hydroxyproline-rich glycoprotein family... 27 8.9 >At1g74050.1 68414.m08576 60S ribosomal protein L6 (RPL6C) similar to 60S ribosomal protein L6 (YL 16 like) GB:CAB57309 from [Cyanophora paradoxa] Length = 233 Score = 53.6 bits (123), Expect = 1e-07 Identities = 30/52 (57%), Positives = 33/52 (63%), Gaps = 4/52 (7%) Frame = -2 Query: 524 TQDPTHLKI----GTVCILLAGRHAGKRVVLVGILPSGLLLVTGPFAFNSCP 381 T PT L+ GTV I+LAGR GKRVV + L SGLLLVTGPF N P Sbjct: 79 TAKPTKLRASITPGTVLIILAGRFKGKRVVFLKQLASGLLLVTGPFKINGVP 130 Score = 50.0 bits (114), Expect = 1e-06 Identities = 29/70 (41%), Positives = 40/70 (57%) Frame = -3 Query: 274 EVRQTYSKRKEGDDIFATKKEKYVPSEQRKTDQKTVDEAVIKAIGARPDKKVLRGYLKAA 95 +V + K+ EG+ A K+EK + +K DQK VD A+IKAI A P+ L+ YL A Sbjct: 163 KVAEKKKKKTEGEFFEAEKEEKKEIPQVKKDDQKAVDAALIKAIEAVPE---LKTYLGAR 219 Query: 94 FGLRSSQYPH 65 F L+ PH Sbjct: 220 FSLKQGMKPH 229 Score = 42.3 bits (95), Expect = 3e-04 Identities = 27/66 (40%), Positives = 36/66 (54%), Gaps = 6/66 (9%) Frame = -1 Query: 387 VPVRRIPQRYVIGTSTRISLGNFKLPKHFNDDYF-----KKNKKCVKRTANA-KRVMTSL 226 VP+RR+ Q YVIGTST++ + L K F+D YF KK KK A K + Sbjct: 129 VPLRRVNQAYVIGTSTKVDISGVTLDK-FDDKYFGKVAEKKKKKTEGEFFEAEKEEKKEI 187 Query: 225 PQKKRN 208 PQ K++ Sbjct: 188 PQVKKD 193 >At1g18540.1 68414.m02313 60S ribosomal protein L6 (RPL6A) similar to 60S ribosomal protein L6 GI:7208784 from [Cicer arietinum] Length = 233 Score = 53.6 bits (123), Expect = 1e-07 Identities = 30/49 (61%), Positives = 32/49 (65%), Gaps = 4/49 (8%) Frame = -2 Query: 515 PTHLKI----GTVCILLAGRHAGKRVVLVGILPSGLLLVTGPFAFNSCP 381 PT LK GTV I+LAGR GKRVV + L SGLLLVTGPF N P Sbjct: 82 PTKLKASITPGTVLIILAGRFKGKRVVFLKQLSSGLLLVTGPFKINGVP 130 Score = 52.4 bits (120), Expect = 3e-07 Identities = 30/70 (42%), Positives = 41/70 (58%) Frame = -3 Query: 274 EVRQTYSKRKEGDDIFATKKEKYVPSEQRKTDQKTVDEAVIKAIGARPDKKVLRGYLKAA 95 +V + K+ EG+ A K+EK +++K DQKTVD A+IK+I A P+ KV YL A Sbjct: 163 KVAEKKKKKTEGEFFEAEKEEKKEIPQEKKEDQKTVDAALIKSIEAVPELKV---YLGAR 219 Query: 94 FGLRSSQYPH 65 F L PH Sbjct: 220 FSLSQGMKPH 229 Score = 41.9 bits (94), Expect = 4e-04 Identities = 27/65 (41%), Positives = 35/65 (53%), Gaps = 6/65 (9%) Frame = -1 Query: 387 VPVRRIPQRYVIGTSTRISLGNFKLPKHFNDDYF-----KKNKKCVKRTANA-KRVMTSL 226 VP+RR+ Q YVIGTST+I + K F+D YF KK KK A K + Sbjct: 129 VPLRRVNQAYVIGTSTKIDISGVNTEK-FDDKYFGKVAEKKKKKTEGEFFEAEKEEKKEI 187 Query: 225 PQKKR 211 PQ+K+ Sbjct: 188 PQEKK 192 >At1g74060.1 68414.m08578 60S ribosomal protein L6 (RPL6B) similar to 60S ribosomal protein L6 (YL 16 like) GB:CAB57309 from [Cyanophora paradoxa] Length = 233 Score = 52.8 bits (121), Expect = 2e-07 Identities = 26/39 (66%), Positives = 28/39 (71%) Frame = -2 Query: 497 GTVCILLAGRHAGKRVVLVGILPSGLLLVTGPFAFNSCP 381 GTV I+LAGR GKRVV + L SGLLLVTGPF N P Sbjct: 92 GTVLIILAGRFKGKRVVFLKQLASGLLLVTGPFKINGVP 130 Score = 50.0 bits (114), Expect = 1e-06 Identities = 29/70 (41%), Positives = 40/70 (57%) Frame = -3 Query: 274 EVRQTYSKRKEGDDIFATKKEKYVPSEQRKTDQKTVDEAVIKAIGARPDKKVLRGYLKAA 95 +V + K+ EG+ A K+EK + +K DQK VD A+IKAI A P+ L+ YL A Sbjct: 163 KVAEKKKKKTEGEFFEAEKEEKKEIPQGKKDDQKAVDAALIKAIEAVPE---LKTYLGAR 219 Query: 94 FGLRSSQYPH 65 F L+ PH Sbjct: 220 FSLKQGMKPH 229 Score = 42.3 bits (95), Expect = 3e-04 Identities = 27/66 (40%), Positives = 36/66 (54%), Gaps = 6/66 (9%) Frame = -1 Query: 387 VPVRRIPQRYVIGTSTRISLGNFKLPKHFNDDYF-----KKNKKCVKRTANA-KRVMTSL 226 VP+RR+ Q YVIGTST++ + L K F+D YF KK KK A K + Sbjct: 129 VPLRRVNQAYVIGTSTKVDISGVTLDK-FDDKYFGKVAEKKKKKTEGEFFEAEKEEKKEI 187 Query: 225 PQKKRN 208 PQ K++ Sbjct: 188 PQGKKD 193 >At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DNA topoisomerase II / DNA gyrase (TOP2) identical to SP|P30182 DNA topoisomerase II (EC 5.99.1.3) {Arabidopsis thaliana} Length = 1473 Score = 33.1 bits (72), Expect = 0.18 Identities = 24/65 (36%), Positives = 32/65 (49%) Frame = -3 Query: 250 RKEGDDIFATKKEKYVPSEQRKTDQKTVDEAVIKAIGARPDKKVLRGYLKAAFGLRSSQY 71 +K G ATK K P+ RK ++TV + AIG P+KKV R + F +SS Sbjct: 1346 KKGGRKPAATKAAK-PPAAPRKRGKQTVASTEVLAIGVSPEKKV-RKMRSSPFNKKSSSV 1403 Query: 70 PHRCA 56 R A Sbjct: 1404 MSRLA 1408 >At5g52730.1 68418.m06545 heavy-metal-associated domain-containing protein contains Pfam profile PF00403: Heavy-metal-associated domain Length = 185 Score = 30.7 bits (66), Expect = 0.95 Identities = 25/81 (30%), Positives = 36/81 (44%), Gaps = 3/81 (3%) Frame = -1 Query: 300 NDDYFKKNKKCVKRTANAKRVMTSLPQKKRN--TFHLSSAKPIRRQSTRL*SKPS-EPDP 130 N + KK KK K A P++ RN T + +P + +S +PS EP+P Sbjct: 87 NYEMTKKLKKICKHVAIIAAEPIREPEQNRNPVTRREPNREPEQNRSRVTRREPSREPEP 146 Query: 129 TRRCSADTSKRPSDSARANIP 67 R A RP +R +IP Sbjct: 147 NRAPLARRESRPRTHSRPSIP 167 >At1g78120.1 68414.m09104 tetratricopeptide repeat (TPR)-containing protein low similarity to SP|Q99615 DnaJ homolog subfamily C member 7 (Tetratricopeptide repeat protein 2) {Homo sapiens}; contains Pfam profile PF00515: TPR Domain Length = 530 Score = 30.3 bits (65), Expect = 1.3 Identities = 18/57 (31%), Positives = 33/57 (57%) Frame = -1 Query: 228 LPQKKRNTFHLSSAKPIRRQSTRL*SKPSEPDPTRRCSADTSKRPSDSARANIPIGA 58 LP+K +T S++P + S + KP + R S++++++ SDSAR +I G+ Sbjct: 60 LPRKSTDT----SSQPKKSDSQKPQQKPKPDENHPRKSSESARKSSDSARKSISSGS 112 >At1g13810.1 68414.m01621 expressed protein ; expression supported by MPSS Length = 303 Score = 30.3 bits (65), Expect = 1.3 Identities = 19/68 (27%), Positives = 33/68 (48%) Frame = -1 Query: 441 WNSAQRSAFSYWTFCFQFVPVRRIPQRYVIGTSTRISLGNFKLPKHFNDDYFKKNKKCVK 262 W + + F +W +P R I + I ++ L FK PKH+++D K + + Sbjct: 230 WEDMKPALFDFWQN--HVLPAREIYNNFDI-KDPQVKLREFK-PKHWHEDCKKIMRGAER 285 Query: 261 RTANAKRV 238 +ANA R+ Sbjct: 286 ISANANRL 293 >At2g29210.1 68415.m03550 splicing factor PWI domain-containing protein contains Pfam profile PF01480: PWI domain Length = 878 Score = 29.9 bits (64), Expect = 1.7 Identities = 12/31 (38%), Positives = 21/31 (67%), Gaps = 1/31 (3%) Frame = -3 Query: 607 PLKRRKSFYPTQEKIRASSGGRPFS-KHVRR 518 P++RR+S P +E++ S GGR S H+++ Sbjct: 525 PVRRRRSLTPDEERVSLSQGGRHTSPSHIKQ 555 >At3g10440.1 68416.m01252 hypothetical protein Length = 556 Score = 29.5 bits (63), Expect = 2.2 Identities = 19/50 (38%), Positives = 29/50 (58%), Gaps = 4/50 (8%) Frame = -1 Query: 183 PIRRQSTRL*SKPSEPDPTRRCSADTSKRPSDSARA---NI-PIGALLNL 46 P+RR+S RL S+ EP + S +T+KR + R+ NI +G + NL Sbjct: 311 PLRRRSARLKSQEPEPSESFHDSIETTKRRRSAIRSAMFNIQELGVIQNL 360 >At5g35338.2 68418.m04194 methyl-CpG-binding domain-containing protein contains Pfam profile PF01429:Methyl-CpG binding domain Length = 155 Score = 28.7 bits (61), Expect = 3.8 Identities = 11/27 (40%), Positives = 19/27 (70%) Frame = -1 Query: 324 NFKLPKHFNDDYFKKNKKCVKRTANAK 244 +F++PK +D+Y KK + VKR ++K Sbjct: 120 SFQIPKPLDDNYVKKRTRPVKRRKSSK 146 >At5g64030.1 68418.m08039 dehydration-responsive protein-related similar to early-responsive to dehydration stress ERD3 protein [Arabidopsis thaliana] GI:15320410; contains Pfam profile PF03141: Putative methyltransferase Length = 829 Score = 27.9 bits (59), Expect = 6.7 Identities = 14/27 (51%), Positives = 18/27 (66%) Frame = -3 Query: 274 EVRQTYSKRKEGDDIFATKKEKYVPSE 194 EVR TYSK KEG + + +K + PSE Sbjct: 796 EVRMTYSKEKEG--LLSVQKSIWRPSE 820 >At5g53930.1 68418.m06710 expressed protein Length = 529 Score = 27.9 bits (59), Expect = 6.7 Identities = 19/68 (27%), Positives = 30/68 (44%) Frame = -1 Query: 300 NDDYFKKNKKCVKRTANAKRVMTSLPQKKRNTFHLSSAKPIRRQSTRL*SKPSEPDPTRR 121 +D KK K+ ++ N + + +KR LSS+ QS + SE D +R Sbjct: 88 DDRLLKKKKRSKRKDENVGKKKKKVVSRKRRKRDLSSSSTSSEQSD---NDGSESDDGKR 144 Query: 120 CSADTSKR 97 S D +R Sbjct: 145 WSRDRGRR 152 >At1g20540.1 68414.m02559 transducin family protein / WD-40 repeat family protein contains 6 WD-40 repeats (PF00400); similar to Rbap46 polypeptide (GI:9454362) [Gallus gallus] Length = 351 Score = 27.9 bits (59), Expect = 6.7 Identities = 14/36 (38%), Positives = 19/36 (52%), Gaps = 2/36 (5%) Frame = -2 Query: 641 WR*EEWGNQNSTPQT*EV--LLPHSGENPCFIWWPS 540 W+ E Q ++PQ V L H G+ C +WWPS Sbjct: 96 WQIPELYGQLNSPQLERVASLDAHVGKINCVLWWPS 131 >At4g23130.2 68417.m03334 receptor-like protein kinase 6 (RLK6) identical to receptor-like protein kinase 6 [Arabidopsis thaliana] GI:13506749; contains Pfam domain PF00069: Protein kinase domain Length = 663 Score = 27.5 bits (58), Expect = 8.9 Identities = 11/23 (47%), Positives = 15/23 (65%) Frame = +3 Query: 600 LRGTVLVPPFFSPPICFTTTVSF 668 + GTVL PP +PP+ +T SF Sbjct: 248 IEGTVLPPPVSAPPLPLVSTPSF 270 >At4g23130.1 68417.m03333 receptor-like protein kinase 6 (RLK6) identical to receptor-like protein kinase 6 [Arabidopsis thaliana] GI:13506749; contains Pfam domain PF00069: Protein kinase domain Length = 659 Score = 27.5 bits (58), Expect = 8.9 Identities = 11/23 (47%), Positives = 15/23 (65%) Frame = +3 Query: 600 LRGTVLVPPFFSPPICFTTTVSF 668 + GTVL PP +PP+ +T SF Sbjct: 248 IEGTVLPPPVSAPPLPLVSTPSF 270 >At3g08670.1 68416.m01007 expressed protein Length = 567 Score = 27.5 bits (58), Expect = 8.9 Identities = 15/43 (34%), Positives = 25/43 (58%) Frame = +3 Query: 117 STFLSGRAPMALITASSTVF*SVLRCSDGTYFSFFVAKMSSPS 245 S+F SGR+P +++ SS S +R S + S A+ S+P+ Sbjct: 168 SSFTSGRSPSSILNTSSASVSSYIRPSSPSSRSSSSARPSTPT 210 >At2g31270.1 68415.m03818 hydroxyproline-rich glycoprotein family protein Length = 571 Score = 27.5 bits (58), Expect = 8.9 Identities = 15/49 (30%), Positives = 26/49 (53%) Frame = -2 Query: 443 VGILPSGLLLVTGPFAFNSCPYAVFLSAM*SAPPPEFHSATSNCQNTSM 297 + ILP+ + V+ F S P + + + +A PPEF S + +TS+ Sbjct: 321 INILPTPVKPVSTLAKFPSTPAIIDSTPVITATPPEFASTPARLMSTSL 369 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,524,903 Number of Sequences: 28952 Number of extensions: 354833 Number of successful extensions: 1130 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 1084 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1130 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1467502800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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