Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV30131
(764 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g15290.1 68416.m01931 3-hydroxybutyryl-CoA dehydrogenase, put... 99 3e-21
At3g06860.1 68416.m00814 fatty acid multifunctional protein (MFP... 79 2e-15
At4g29010.1 68417.m04147 abnormal inflorescence meristem 1 / fat... 75 5e-14
At2g34090.1 68415.m04173 expressed protein similar to GP|130183... 31 1.1
At1g65050.1 68414.m07374 meprin and TRAF homology domain-contain... 31 1.1
At4g39450.1 68417.m05582 expressed protein 29 4.5
At2g34090.2 68415.m04174 expressed protein similar to GP|130183... 28 5.9
>At3g15290.1 68416.m01931 3-hydroxybutyryl-CoA dehydrogenase,
putative similar to S(+)-beta-hydroxybutyryl CoA
dehydrogenase (3-hydroxybutyryl-CoA dehydrogenase)
[Paracoccus denitrificans] GI:12003356; contains Pfam
profiles PF02737: 3-hydroxyacyl-CoA dehydrogenase NAD
binding, PF00725: 3-hydroxyacyl-CoA dehydrogenase
C-terminal
Length = 294
Score = 99.1 bits (236), Expect = 3e-21
Identities = 43/80 (53%), Positives = 62/80 (77%)
Frame = -3
Query: 762 LVVEAIVENIGVKHKLFKQLDGVAPSHTIFASNTSSLSITEIASVVKRKDKFGGLHFFNP 583
++VEAIVE+ +K KLFK LDG+A S I ASNTSS+SIT +AS +R + G+HF NP
Sbjct: 86 IIVEAIVESEDIKKKLFKDLDGIAKSSAILASNTSSISITRLASATRRPSQVIGMHFMNP 145
Query: 582 VPVMRLLEVVKGSETSEATY 523
P+M+L+E+++G++TSE T+
Sbjct: 146 PPIMKLVEIIRGADTSEETF 165
Score = 74.1 bits (174), Expect = 9e-14
Identities = 37/79 (46%), Positives = 47/79 (59%)
Frame = -2
Query: 493 GKTCITCKDTPGFVVNRLLVPYICEAIRLYERGDASARDIDIAMKLGAGYPMGPLELADY 314
GKT + +D GFVVNR+L+P I EA G A+ DID MK G +PMGPLELAD
Sbjct: 176 GKTTVCSQDYAGFVVNRILMPMINEAFHTLYTGVATKEDIDSGMKHGTNHPMGPLELADL 235
Query: 313 VGLDTNKFILDGWHKKFPD 257
+GLD ++ H+ D
Sbjct: 236 IGLDVCLSVMKVLHEGLGD 254
Score = 28.7 bits (61), Expect = 4.5
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = -1
Query: 260 GSQLFKPIPLLEKLVAEGKLGVKTGEGFY 174
G + P PLL + V G+LG K G G Y
Sbjct: 253 GDSKYAPCPLLVQYVDAGRLGRKRGVGVY 281
>At3g06860.1 68416.m00814 fatty acid multifunctional protein (MFP2)
identical to fatty acid multifunctional protein (AtMFP2)
GB:AF123254 [gi:4337027] (Arabidopsis thaliana) (fatty
acid beta-oxidation); contains Pfam profiles PF02737
(3-hydroxyacyl-CoA dehydrogenase, NAD binding domain),
PF00378 (enoyl-CoA hydratase/isomerase family protein),
PF00725 (3-hydroxyacyl-CoA dehydrogenase)
Length = 725
Score = 79.4 bits (187), Expect = 2e-15
Identities = 36/95 (37%), Positives = 58/95 (61%), Gaps = 1/95 (1%)
Frame = -3
Query: 762 LVVEAIVENIGVKHKLFKQLDGVAPSHTIFASNTSSLSITEIASVVKRKDKFGGLHFFNP 583
+V+EA++ENI +K ++F L+ P H I ASNTS++ + +I K +D+ G HFF+P
Sbjct: 394 MVIEAVIENISLKQQIFADLEKYCPQHCILASNTSTIDLNKIGERTKSQDRIVGAHFFSP 453
Query: 582 VPVMRLLEVVKGSETSEATYKTMMD-GESRSARPV 481
+M LLE+V+ + TS ++D G+ PV
Sbjct: 454 AHIMPLLEIVRTNHTSAQVIVDLLDVGKKIKKTPV 488
Score = 44.4 bits (100), Expect = 8e-05
Identities = 28/66 (42%), Positives = 34/66 (51%)
Frame = -2
Query: 505 GKSVGKTCITCKDTPGFVVNRLLVPYICEAIRLYERGDASARDIDIAMKLGAGYPMGPLE 326
GK + KT + + GF VNR+ PY A+ L E G A ID A+ G PMGP
Sbjct: 480 GKKIKKTPVVVGNCTGFAVNRMFFPYTQAAMFLVECG-ADPYLIDRAIS-KFGMPMGPFR 537
Query: 325 LADYVG 308
L D VG
Sbjct: 538 LCDLVG 543
Score = 33.5 bits (73), Expect = 0.16
Identities = 22/68 (32%), Positives = 33/68 (48%), Gaps = 4/68 (5%)
Frame = -2
Query: 454 VVNRLLVPYICEAIRLYERGDA-SARDIDIAMKLGAGYPM---GPLELADYVGLDTNKFI 287
++ P + EA R++ G A A D+DIA +G G+P G + AD +G
Sbjct: 627 IIEMTFFPVVNEACRVFAEGIAVKAADLDIAGIMGMGFPPYRGGIMFWADSIGSKYIYSR 686
Query: 286 LDGWHKKF 263
LD W K +
Sbjct: 687 LDEWSKAY 694
>At4g29010.1 68417.m04147 abnormal inflorescence meristem 1 / fatty
acid multifunctional protein (AIM1) identical to
gi:4337025; contains Pfam profiles PF02737
(3-hydroxyacyl-CoA dehydrogenase, NAD binding domain),
PF00378 (enoyl-CoA hydratase/isomerase family protein),
PF00725 (3-hydroxyacyl-CoA dehydrogenase)
Length = 721
Score = 74.9 bits (176), Expect = 5e-14
Identities = 33/84 (39%), Positives = 51/84 (60%)
Frame = -3
Query: 762 LVVEAIVENIGVKHKLFKQLDGVAPSHTIFASNTSSLSITEIASVVKRKDKFGGLHFFNP 583
+V+EA++ENI +K +FK+++ V H I ASNTS++ + I KD+ G HFF+P
Sbjct: 391 MVIEAVIENIQLKQNIFKEIEKVCSPHCILASNTSTIDLDVIGEKTNSKDRIVGAHFFSP 450
Query: 582 VPVMRLLEVVKGSETSEATYKTMM 511
+M LLE+V+ TS +M
Sbjct: 451 AHLMPLLEIVRSKNTSAQVILDLM 474
Score = 34.3 bits (75), Expect = 0.090
Identities = 23/66 (34%), Positives = 31/66 (46%)
Frame = -2
Query: 505 GKSVGKTCITCKDTPGFVVNRLLVPYICEAIRLYERGDASARDIDIAMKLGAGYPMGPLE 326
GK++ K + + GF VNR PY A L G R ID ++ G P+GP +
Sbjct: 477 GKAIKKVPVVVGNCIGFAVNRTFFPYSQAAHMLANLGVDLFR-ID-SVITSFGLPLGPFQ 534
Query: 325 LADYVG 308
L D G
Sbjct: 535 LGDLAG 540
>At2g34090.1 68415.m04173 expressed protein similar to
GP|1301832|gnl|PID|e239862|Z71287
Length = 445
Score = 30.7 bits (66), Expect = 1.1
Identities = 17/55 (30%), Positives = 28/55 (50%), Gaps = 1/55 (1%)
Frame = -2
Query: 514 DGWGKSVGKTCITCKDTPGFVVNRLLVPYICE-AIRLYERGDASARDIDIAMKLG 353
D ++ G T I K+ PG+ +N LLVP E A+ L + + D+ + +G
Sbjct: 369 DALNRTHGDTNIRLKNPPGYYINTLLVPKDGEIAVDLKQLSEQGIYDVRVVESVG 423
>At1g65050.1 68414.m07374 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein low similarity
to ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 228
Score = 30.7 bits (66), Expect = 1.1
Identities = 17/47 (36%), Positives = 26/47 (55%), Gaps = 3/47 (6%)
Frame = -2
Query: 508 WGKSVGKTCITCKD-TPGFVVNRLL--VPYICEAIRLYERGDASARD 377
WG S T KD GF+++RL P + + ++LY +GD+ A D
Sbjct: 114 WGLSQVLPVETLKDRAKGFILSRLFHNPPLLVKTLKLYPKGDSEADD 160
>At4g39450.1 68417.m05582 expressed protein
Length = 1553
Score = 28.7 bits (61), Expect = 4.5
Identities = 18/65 (27%), Positives = 33/65 (50%), Gaps = 1/65 (1%)
Frame = +2
Query: 392 ISSFVQPNGFTYVRHQQSIHNETRGVLASDTGLADRLSPSIIVLYVASDV-SLPLTTSSK 568
ISSF + NG + HQ+S+ ++S+ L L+ ++ Y D+ S+P +
Sbjct: 174 ISSFYKSNGNADMAHQKSLKRSMFHSVSSEDDLMGSLARALANEYAYPDISSVPKQKQNP 233
Query: 569 RITGT 583
I+G+
Sbjct: 234 SISGS 238
>At2g34090.2 68415.m04174 expressed protein similar to
GP|1301832|gnl|PID|e239862|Z71287
Length = 471
Score = 28.3 bits (60), Expect = 5.9
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = -2
Query: 514 DGWGKSVGKTCITCKDTPGFVVNRLLVP 431
D ++ G T I K+ PG+ +N LLVP
Sbjct: 369 DALNRTHGDTNIRLKNPPGYYINTLLVP 396
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,767,574
Number of Sequences: 28952
Number of extensions: 345743
Number of successful extensions: 807
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 779
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 804
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1712086600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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